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Mesenchymal Stem\/Stromal Cell (MSC) migration towards degenerated IVD may represent a viable strategy to promote tissue repair\/regeneration. Here, human MSCs (hMSCs) were seeded on top of cartilaginous endplates (CEP) of nucleotomized IVDs of bovine origin and cultured <jats:italic>ex vivo<\/jats:italic> up to 3 weeks. hMSCs migrated from CEP towards the lesion area and significantly increased expression of collagen type II and aggrecan in IVD, namely in the nucleus pulposus. Concomitantly, hMSCs stimulated the production of growth factors, promoters of ECM synthesis, such as fibroblast growth factor 6 (FGF-6) and 7 (FGF-7), platelet-derived growth factor receptor (PDGF-R), granulocyte-macrophage colony-stimulating factor (GM-CSF) and insulin-like growth factor 1 receptor (IGF-1sR). Overall, our results demonstrate that CEP can be an alternative route to MSC-based therapies for IVD regeneration through ECM remodeling, thus opening new perspectives on endogenous repair capacity through MSC recruitment.<\/jats:p>","DOI":"10.1038\/srep33836","type":"journal-article","created":{"date-parts":[[2016,9,22]],"date-time":"2016-09-22T09:01:48Z","timestamp":1474534908000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Mesenchymal Stem\/Stromal Cells seeded on cartilaginous endplates promote Intervertebral Disc Regeneration through Extracellular Matrix Remodeling"],"prefix":"10.1038","volume":"6","author":[{"given":"Catarina Leite","family":"Pereira","sequence":"first","affiliation":[]},{"given":"Graciosa Q.","family":"Teixeira","sequence":"additional","affiliation":[]},{"given":"Cl\u00e1udia","family":"Ribeiro-Machado","sequence":"additional","affiliation":[]},{"given":"Joana","family":"Caldeira","sequence":"additional","affiliation":[]},{"given":"Madalena","family":"Costa","sequence":"additional","affiliation":[]},{"given":"Francisco","family":"Figueiredo","sequence":"additional","affiliation":[]},{"given":"Rui","family":"Fernandes","sequence":"additional","affiliation":[]},{"given":"Paulo","family":"Aguiar","sequence":"additional","affiliation":[]},{"given":"Sibylle","family":"Grad","sequence":"additional","affiliation":[]},{"given":"M\u00e1rio A.","family":"Barbosa","sequence":"additional","affiliation":[]},{"given":"Raquel M.","family":"Gon\u00e7alves","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2016,9,22]]},"reference":[{"key":"BFsrep33836_CR1","doi-asserted-by":"publisher","first-page":"480","DOI":"10.1111\/j.1469-7580.2012.01521.x","volume":"221","author":"G Pattappa","year":"2012","unstructured":"Pattappa, G. et al. 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