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Dies1 inhibition in a melanoma-mouse model led to increased tumour-infiltrating T-cells and decreased tumour growth, emphasizing <jats:italic>Dies1<\/jats:italic> relevance in tumour-microenvironment. Dies1 is involved in cell de\/differentiation, inflammation and cancer processes, which mimic those associated with Epithelial-to-Mesenchymal-Transition (EMT). Despite this axis linking <jats:italic>Dies1<\/jats:italic> with EMT and cancer, its expression, modulation and relevance in these contexts is unknown. To address this, we analysed <jats:italic>Dies1<\/jats:italic> expression, its regulation by promoter-methylation and <jats:italic>miR-125a-5p<\/jats:italic> overexpression, and its association with BMP-pathway downstream-effectors, in a TGF\u03b21-induced EMT-model, cancer cell-lines and primary samples. We detected promoter-methylation as a mechanism controlling <jats:italic>Dies1<\/jats:italic> expression in our EMT-model and in several cancer cell-lines. We showed that the relationship between <jats:italic>Dies1<\/jats:italic> expression and BMP-pathway effectors observed in the EMT-model, was not present in all cell-lines, suggesting that <jats:italic>Dies1<\/jats:italic> has other cell-specific effectors, beyond the BMP-pathway. We further demonstrated that: <jats:italic>Dies1<\/jats:italic> expression loss is a recurrent event in GC, caused by promoter methylation and\/or <jats:italic>miR-125a-5p<\/jats:italic> overexpression and; GC-microenvironment myofibroblasts overexpress <jats:italic>Dies1.<\/jats:italic> Our findings highlight <jats:italic>Dies1<\/jats:italic> as a novel player in GC, with distinct roles within tumour cells and in the tumour-microenvironment.<\/jats:p>","DOI":"10.1038\/srep34860","type":"journal-article","created":{"date-parts":[[2016,10,10]],"date-time":"2016-10-10T11:27:02Z","timestamp":1476098822000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Dies1\/VISTA expression loss is a recurrent event in gastric cancer due to epigenetic regulation"],"prefix":"10.1038","volume":"6","author":[{"given":"Patr\u00edcia","family":"Oliveira","sequence":"first","affiliation":[]},{"given":"Joana","family":"Carvalho","sequence":"additional","affiliation":[]},{"given":"Sara","family":"Rocha","sequence":"additional","affiliation":[]},{"given":"Mafalda","family":"Azevedo","sequence":"additional","affiliation":[]},{"given":"In\u00eas","family":"Reis","sequence":"additional","affiliation":[]},{"given":"V\u00e2nia","family":"Camilo","sequence":"additional","affiliation":[]},{"given":"B\u00e1rbara","family":"Sousa","sequence":"additional","affiliation":[]},{"given":"Sofia","family":"Valente","sequence":"additional","affiliation":[]},{"given":"Joana","family":"Paredes","sequence":"additional","affiliation":[]},{"given":"Raquel","family":"Almeida","sequence":"additional","affiliation":[]},{"given":"David","family":"Huntsman","sequence":"additional","affiliation":[]},{"given":"Carla","family":"Oliveira","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2016,10,10]]},"reference":[{"key":"BFsrep34860_CR1","doi-asserted-by":"publisher","first-page":"D662","DOI":"10.1093\/nar\/gku1010","volume":"43","author":"F Cunningham","year":"2015","unstructured":"Cunningham, F. et al. 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