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In this study 13 sulfated polyphenols were synthesized and tested for antifouling potential using the anti-settlement activity of mussel (<jats:italic>Mytilus galloprovincialis<\/jats:italic>) plantigrade post-larvae and bacterial growth inhibition towards four biofilm-forming bacterial strains. Results show that some of these Nature-inspired compounds were bioactive, particularly rutin persulfate (2), 3,6-bis(<jats:italic>\u03b2<\/jats:italic>-D-glucopyranosyl) xanthone persulfate (6), and gallic acid persulfate (12) against the settlement of plantigrades. The chemical precursors of sulfated compounds <jats:bold>2<\/jats:bold> and <jats:bold>12<\/jats:bold> were also tested for anti-settlement activity and it was possible to conclude that bioactivity is associated with sulfation. While compound <jats:bold>12<\/jats:bold> showed the most promising anti-settlement activity (EC<jats:sub>50<\/jats:sub>\u2009=\u20098.95\u2009\u03bcg.mL<jats:sup>\u22121<\/jats:sup>), compound <jats:bold>2<\/jats:bold> also caused the higher level of growth inhibition in bacteria <jats:italic>Vibrio harveyi<\/jats:italic> (EC<jats:sub>20<\/jats:sub>\u2009=\u200912.5\u2009\u03bcg.mL<jats:sup>\u22121<\/jats:sup>). All the three bioactive compounds <jats:bold>2<\/jats:bold>, <jats:bold>6<\/jats:bold>, and <jats:bold>12<\/jats:bold> were also found to be nontoxic to the non target species <jats:italic>Artemia salina<\/jats:italic> (&lt;10% mortality at 250\u2009\u03bcM) and <jats:italic>Vibrio fischeri<\/jats:italic> (LC<jats:sub>50<\/jats:sub>\u2009&gt;\u20091000\u2009\u03bcg.mL<jats:sup>\u22121<\/jats:sup>). This study put forward the relevance of synthesizing non-natural sulfated small molecules to generate new nontoxic antifouling agents.<\/jats:p>","DOI":"10.1038\/srep42424","type":"journal-article","created":{"date-parts":[[2017,2,13]],"date-time":"2017-02-13T11:05:34Z","timestamp":1486983934000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":65,"title":["Antifouling potential of Nature-inspired sulfated compounds"],"prefix":"10.1038","volume":"7","author":[{"given":"Joana R.","family":"Almeida","sequence":"first","affiliation":[]},{"given":"Marta","family":"Correia-da-Silva","sequence":"additional","affiliation":[]},{"given":"Em\u00edlia","family":"Sousa","sequence":"additional","affiliation":[]},{"given":"Jorge","family":"Antunes","sequence":"additional","affiliation":[]},{"given":"Madalena","family":"Pinto","sequence":"additional","affiliation":[]},{"given":"Vitor","family":"Vasconcelos","sequence":"additional","affiliation":[]},{"given":"Isabel","family":"Cunha","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2017,2,13]]},"reference":[{"key":"BFsrep42424_CR1","first-page":"10","volume":"49","author":"ME Callow","year":"2002","unstructured":"Callow, M. 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