{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T02:42:15Z","timestamp":1773801735660,"version":"3.50.1"},"reference-count":70,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2017,2,20]],"date-time":"2017-02-20T00:00:00Z","timestamp":1487548800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2017,2,20]],"date-time":"2017-02-20T00:00:00Z","timestamp":1487548800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Mediterranean Sea ecosystems are considered as hotspots of biological introductions, exposed to possible negative effects of non\u2013indigenous species. In such temperate marine ecosystems, macroalgae may be dominant, with a great percentage of their diversity represented by introduced species. Their interaction with temperate indigenous benthic organisms have been poorly investigated. To provide new insights, we performed an experimental study on the interaction between the introduced proliferative red alga <jats:italic>Asparagopsis taxiformis<\/jats:italic> and the indigenous Mediterranean coral <jats:italic>Astroides calycularis<\/jats:italic>. The biological response measurements included meta\u2013barcoding of the associated microbial communities and metabolomic fingerprinting of both species. Significant changes were detected among both associated microbial communities, the interspecific differences decreasing with stronger host interaction. No short term effects of the macroalga on the coral health, neither on its polyp activity or its metabolism, were detected. In contrast, the contact interaction with the coral induced a change in the macroalgal metabolomic fingerprint with a significant increase of its bioactivity against the marine bacteria <jats:italic>Aliivibrio fischeri<\/jats:italic>. This induction was related to the expression of bioactive metabolites located on the macroalgal surface, a phenomenon which might represent an immediate defensive response of the macroalga or an allelopathic offense against coral.<\/jats:p>","DOI":"10.1038\/srep42625","type":"journal-article","created":{"date-parts":[[2017,2,20]],"date-time":"2017-02-20T10:34:26Z","timestamp":1487586866000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["The interaction between the proliferating macroalga Asparagopsis taxiformis and the coral Astroides calycularis induces changes in microbiome and metabolomic fingerprints"],"prefix":"10.1038","volume":"7","author":[{"given":"St\u00e9phane","family":"Greff","sequence":"first","affiliation":[]},{"given":"T\u00e2nia","family":"Aires","sequence":"additional","affiliation":[]},{"given":"Ester A.","family":"Serr\u00e3o","sequence":"additional","affiliation":[]},{"given":"Aschwin H.","family":"Engelen","sequence":"additional","affiliation":[]},{"given":"Olivier P.","family":"Thomas","sequence":"additional","affiliation":[]},{"given":"Thierry","family":"P\u00e9rez","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2017,2,20]]},"reference":[{"key":"BFsrep42625_CR1","doi-asserted-by":"publisher","first-page":"263","DOI":"10.1016\/j.pocean.2004.02.014","volume":"60","author":"JW McManus","year":"2004","unstructured":"McManus, J. W. & Polsenberg, J. F. Coral\u2013algal phase shifts on coral reefs: ecological and environmental aspects. Prog. Oceanogr. 60, 263\u2013279, doi: 10.1016\/j.pocean.2004.02.014 (2004).","journal-title":"Prog. Oceanogr."},{"key":"BFsrep42625_CR2","doi-asserted-by":"publisher","first-page":"360","DOI":"10.1016\/j.cub.2006.12.049","volume":"17","author":"TP Hughes","year":"2007","unstructured":"Hughes, T. P. et al. Phase shifts, herbivory, and the resilience of coral reefs to climate change. Curr. Biol. 17, 360\u2013365, doi: 10.1016\/j.cub.2006.12.049 (2007).","journal-title":"Curr. Biol."},{"key":"BFsrep42625_CR3","doi-asserted-by":"publisher","first-page":"1737","DOI":"10.1126\/science.1152509","volume":"318","author":"O Hoegh-Guldberg","year":"2007","unstructured":"Hoegh-Guldberg, O. et al. Coral reefs under rapid climate change and ocean acidification. Science 318, 1737\u20131742, doi: 10.1126\/science.1152509 (2007).","journal-title":"Science"},{"key":"BFsrep42625_CR4","doi-asserted-by":"publisher","first-page":"400","DOI":"10.1007\/s003380000129","volume":"19","author":"L McCook","year":"2001","unstructured":"McCook, L., Jompa, J. & Diaz-Pulido, G. Competition between corals and algae on coral reefs: a review of evidence and mechanisms. Coral Reefs 19, 400\u2013417, doi: 10.1007\/s003380000129 (2001).","journal-title":"Coral Reefs"},{"key":"BFsrep42625_CR5","doi-asserted-by":"publisher","first-page":"2647","DOI":"10.1007\/s10530-012-0261-6","volume":"14","author":"E Cebrian","year":"2012","unstructured":"Cebrian, E., Linares, C., Marschal, C. & Garrabou, J. Exploring the effects of invasive algae on the persistence of gorgonian populations. Biol. Invasions 14, 2647\u20132656, doi: 10.1007\/s10530-012-0261-6 (2012).","journal-title":"Biol. Invasions"},{"key":"BFsrep42625_CR6","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1016\/0022-0981(93)90181-M","volume":"167","author":"JA Coyer","year":"1993","unstructured":"Coyer, J. A., Ambrose, R. F., Engle, J. M. & Carroll, J. C. Interactions between corals and algae on a temperate zone rocky reef: mediation by sea urchins. J. Exp. Mar. Biol. Ecol. 167, 21\u201337, doi: 10.1016\/0022-0981(93)90181-M (1993).","journal-title":"J. Exp. Mar. Biol. Ecol."},{"key":"BFsrep42625_CR7","doi-asserted-by":"publisher","first-page":"1599","DOI":"10.1007\/s10530-013-0594-9","volume":"16","author":"D Kersting","year":"2014","unstructured":"Kersting, D., Ballesteros, E., De Caralt, S. & Linares, C. Invasive macrophytes in a marine reserve (Columbretes Islands, NW Mediterranean): spread dynamics and interactions with the endemic scleractinian coral Cladocora caespitosa . Biol. Invasions 16, 1599\u20131610, doi: 10.1007\/s10530-013-0594-9 (2014).","journal-title":"Biol. Invasions"},{"key":"BFsrep42625_CR8","doi-asserted-by":"publisher","first-page":"e11842","DOI":"10.1371\/journal.pone.0011842","volume":"5","author":"M Coll","year":"2010","unstructured":"Coll, M. et al. The biodiversity of the Mediterranean Sea: estimates, patterns, and threats. PLoS One 5, e11842, doi: 10.1371\/journal.pone.0011842 (2010).","journal-title":"PLoS One"},{"key":"BFsrep42625_CR9","doi-asserted-by":"publisher","first-page":"391","DOI":"10.3391\/ai.2014.9.4.01","volume":"9","author":"S Katsanevakis","year":"2014","unstructured":"Katsanevakis, S. et al. Impacts of invasive alien marine species on ecosystem services and biodiversity: a pan-European review. Aquat. Invasions 9, 391\u2013423, doi: 10.3391\/ai.2014.9.4.01 (2014).","journal-title":"Aquat. Invasions"},{"key":"BFsrep42625_CR10","doi-asserted-by":"publisher","first-page":"397","DOI":"10.1515\/bot.2007.044","volume":"50","author":"B Schaffelke","year":"2007","unstructured":"Schaffelke, B. & Hewitt, C. L. Impacts of introduced seaweeds. Bot. Mar. 50, 397\u2013417, doi: 10.1515\/bot.2007.044 (2007).","journal-title":"Bot. Mar"},{"key":"BFsrep42625_CR11","doi-asserted-by":"crossref","first-page":"5","DOI":"10.24310\/abm.v33i0.6963","volume":"33","author":"M Altamirano","year":"2008","unstructured":"Altamirano, M. et al. The invasive species Asparagopsis taxiformis (Bonnemaisoniales, Rhodophyta) on Andalusian coasts (Southern Spain): reproductive stages, new records and invaded communities. Acta Bot. Malacit. 33, 5\u201315 (2008).","journal-title":"Acta Bot. Malacit"},{"key":"BFsrep42625_CR12","doi-asserted-by":"publisher","first-page":"32","DOI":"10.1016\/S0025-326X(01)00150-3","volume":"44","author":"CF Boudouresque","year":"2002","unstructured":"Boudouresque, C. F. & Verlaque, M. Biological pollution in the Mediterranean Sea: invasive versus introduced macrophytes. Mar. Pollut. Bull. 44, 32\u201338, doi: 10.1016\/S0025-326X(01)00150-3 (2002).","journal-title":"Mar. Pollut. Bull."},{"key":"BFsrep42625_CR13","doi-asserted-by":"publisher","first-page":"e103826","DOI":"10.1371\/journal.pone.0103826","volume":"9","author":"L Dijoux","year":"2014","unstructured":"Dijoux, L., Viard, F. & Payri, C. The more we search, the more we find: discovery of a new lineage and a new species complex in the genus Asparagopsis . PLoS One 9, e103826, doi: 10.1371\/journal.pone.0103826 (2014).","journal-title":"PLoS One"},{"key":"BFsrep42625_CR14","doi-asserted-by":"crossref","first-page":"185","DOI":"10.24310\/abm.v24i0.8524","volume":"24","author":"M Altamirano","year":"1999","unstructured":"Altamirano, M. Nuevas citas para la flora marina del archipi\u00e9lago de las Islas Chafarinas. Acta Bot. Malacit. 24, 185\u2013187 (1999).","journal-title":"Acta Bot. Malacit"},{"key":"BFsrep42625_CR15","doi-asserted-by":"crossref","first-page":"165","DOI":"10.24310\/abm.v35i0.2861","volume":"35","author":"M Altamirano","year":"2010","unstructured":"Altamirano, M., de la Rosa, J., Zanolla, M., Souza-Egipsy, V. & Diaz, J. New records for the benthic marine flora of Chafarinas Islands (Alboran Sea, Western Mediterranean). Acta Bot. Malacit. 35, 165\u2013167 (2010).","journal-title":"Acta Bot. Malacit"},{"key":"BFsrep42625_CR16","unstructured":"Convention for the protection of the marine environment and the coastal region of the Mediterranean. (Barcelona, Spain, 1976)."},{"key":"BFsrep42625_CR17","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.dsr.2015.02.006","volume":"100","author":"O Pasqueron de Fommervault","year":"2015","unstructured":"Pasqueron de Fommervault, O., Migon, C., D\u2019Ortenzio, F., Ribera d\u2019Alcal\u00e0, M. & Coppola, L. Temporal variability of nutrient concentrations in the northwestern Mediterranean sea (DYFAMED time-series station). Deep-Sea Res. Part I Oceanogr. Res. Pap. 100, 1\u201312, doi: 10.1016\/j.dsr.2015.02.006 (2015).","journal-title":"Deep-Sea Res. Part I Oceanogr. Res. Pap"},{"key":"BFsrep42625_CR18","unstructured":"Hansen, H. P. & Koroleff, F. In Methods of Seawater Analysis 159\u2013228 (Wiley-VCH Verlag GmbH, 2007)."},{"key":"BFsrep42625_CR19","doi-asserted-by":"publisher","first-page":"205","DOI":"10.1016\/j.phytol.2013.01.005","volume":"6","author":"N Cachet","year":"2013","unstructured":"Cachet, N., Loffredo, L., Vicente Oscar, O. & Thomas, O. P. Chemical diversity in the scleractinian coral Astroides calycularis . Phytochem Lett. 6, 205\u2013208, doi: 10.1016\/j.phytol.2013.01.005 (2013).","journal-title":"Phytochem Lett"},{"key":"BFsrep42625_CR20","doi-asserted-by":"publisher","first-page":"950","DOI":"10.1007\/bf01951683","volume":"43","author":"A Aiello","year":"1987","unstructured":"Aiello, A., Fattorusso, E., Magno, S., Misuraca, G. & Novellino, E. 2-Amino-6-[(1\u2032R,2\u2032S)-1\u2032,2\u2032-dihydroxypropyl]-3-methyl-pterin-4-one, a biologically active metabolite from the anthozoan Astroides calycularis Pallas. Experientia 43, 950\u2013952, doi: 10.1007\/bf01951683 (1987).","journal-title":"Experientia"},{"key":"BFsrep42625_CR21","doi-asserted-by":"publisher","first-page":"924","DOI":"10.1021\/np50042a006","volume":"48","author":"E Fattorusso","year":"1985","unstructured":"Fattorusso, E., Lanzotti, V., Magno, S. & Novellino, E. Tryptophan derivatives from a Mediterranean anthozoan, Astroides calycularis . J. Nat. Prod. 48, 924\u2013927, doi: 10.1021\/np50042a006 (1985).","journal-title":"J. Nat. Prod"},{"key":"BFsrep42625_CR22","unstructured":"Johnson, B. T. In Small-scale Freshwater Toxicity Investigations (eds Christian Blaise & Jean-Fran\u00e7ois F\u00e9rard ) Ch. 2, 69\u2013105 (Springer Netherlands, 2005)."},{"key":"BFsrep42625_CR23","doi-asserted-by":"publisher","first-page":"199","DOI":"10.1134\/s1063074008040019","volume":"34","author":"EA Titlyanov","year":"2008","unstructured":"Titlyanov, E. A. & Titlyanova, T. V. Coral\u2013algal competition on damaged reefs. Russ. J. Mar. Biol. 34, 199\u2013219, doi: 10.1134\/s1063074008040019 (2008).","journal-title":"Russ. J. Mar. Biol."},{"key":"BFsrep42625_CR24","doi-asserted-by":"publisher","first-page":"2085","DOI":"10.1890\/0012-9658(1999)080[2085:macptr]2.0.co;2","volume":"80","author":"JJ Stachowicz","year":"1999","unstructured":"Stachowicz, J. J. & Hay, M. E. Mutualism and coral persistence: the role of herbivore resistance to algal chemical defense. Ecology 80, 2085\u20132101, doi: 10.1890\/0012-9658(1999)080[2085:macptr]2.0.co;2 (1999).","journal-title":"Ecology"},{"key":"BFsrep42625_CR25","doi-asserted-by":"publisher","first-page":"323","DOI":"10.2307\/2963521","volume":"66","author":"MW Miller","year":"1996","unstructured":"Miller, M. W. & Hay, M. E. Coral-seaweed-grazer-nutrient interactions on temperate reefs. Ecol. Monogr. 66, 323\u2013344, doi: 10.2307\/2963521 (1996).","journal-title":"Ecol. Monogr"},{"key":"BFsrep42625_CR26","doi-asserted-by":"publisher","first-page":"371","DOI":"10.1111\/j.1574-6941.2008.00644.x","volume":"67","author":"M Shnit-Orland","year":"2009","unstructured":"Shnit-Orland, M. & Kushmaro, A. Coral mucus-associated bacteria: a possible first line of defense. FEMS Microbiol. Ecol. 67, 371\u2013380, doi: 10.1111\/j.1574-6941.2008.00644.x (2009).","journal-title":"FEMS Microbiol. Ecol"},{"key":"BFsrep42625_CR27","doi-asserted-by":"publisher","first-page":"343","DOI":"10.1046\/j.1462-2920.2000.00107.x","volume":"2","author":"S Egan","year":"2000","unstructured":"Egan, S., Thomas, T., Holmstr\u00f6m, C. & Kjelleberg, S. Phylogenetic relationship and antifouling activity of bacterial epiphytes from the marine alga Ulva lactuca . Environ. Microbiol. 2, 343\u2013347, doi: 10.1046\/j.1462-2920.2000.00107.x (2000).","journal-title":"Environ. Microbiol."},{"key":"BFsrep42625_CR28","doi-asserted-by":"publisher","first-page":"621","DOI":"10.1016\/j.tim.2012.08.004","volume":"20","author":"KL Barott","year":"2012","unstructured":"Barott, K. L. & Rohwer, F. L. Unseen players shape benthic competition on coral reefs. Trends Microbiol. 20, 621\u2013628, doi: 10.1016\/j.tim.2012.08.004 (2012).","journal-title":"Trends Microbiol."},{"key":"BFsrep42625_CR29","doi-asserted-by":"publisher","first-page":"1647","DOI":"10.1126\/science.1155725","volume":"320","author":"RE Ley","year":"2008","unstructured":"Ley, R. E. et al. Evolution of mammals and their gut microbes. Science 320, 1647\u20131651, doi: 10.1126\/science.1155725 (2008).","journal-title":"Science"},{"key":"BFsrep42625_CR30","doi-asserted-by":"publisher","first-page":"480","DOI":"10.1038\/nature07540","volume":"457","author":"PJ Turnbaugh","year":"2009","unstructured":"Turnbaugh, P. J. et al. A core gut microbiome in obese and lean twins. Nature 457, 480\u2013484, doi: 10.1038\/nature07540 (2009).","journal-title":"Nature"},{"key":"BFsrep42625_CR31","doi-asserted-by":"crossref","unstructured":"Aires, T., Serr\u00e3o, E. A. & Engelen, A. H. Host and environmental specificity in bacterial communities associated to two highly invasive marine species (genus Asparagopsis). Front. Microbiol. 7, doi: 10.3389\/fmicb.2016.00559 (2016).","DOI":"10.3389\/fmicb.2016.00559"},{"key":"BFsrep42625_CR32","doi-asserted-by":"publisher","first-page":"1192","DOI":"10.1111\/j.1462-2920.2010.02419.x","volume":"13","author":"KL Barott","year":"2011","unstructured":"Barott, K. L. et al. Microbial diversity associated with four functional groups of benthic reef algae and the reef-building coral Montastraea annularis . Environ. Microbiol. 13, 1192\u20131204, doi: 10.1111\/j.1462-2920.2010.02419.x (2011).","journal-title":"Environ. Microbiol"},{"key":"BFsrep42625_CR33","doi-asserted-by":"publisher","first-page":"181","DOI":"10.3354\/ab00149","volume":"5","author":"T Lachnit","year":"2009","unstructured":"Lachnit, T., Bl\u00fcmel, M., Imhoff, J. F. & Wahl, M. Specific epibacterial communities on macroalgae: phylogeny matters more than habitat. Aquat. Biol. 5, 181\u2013186, doi: 10.3354\/ab00149 (2009).","journal-title":"Aquat. Biol"},{"key":"BFsrep42625_CR34","doi-asserted-by":"publisher","first-page":"e10401","DOI":"10.1371\/journal.pone.0010401","volume":"5","author":"ECE Kvennefors","year":"2010","unstructured":"Kvennefors, E. C. E., Sampayo, E., Ridgway, T., Barnes, A. C. & Hoegh-Guldberg, O. Bacterial communities of two ubiquitous Great Barrier Reef corals reveals both site- and species-specificity of common bacterial associates. PLoS One 5, e10401, doi: 10.1371\/journal.pone.0010401 (2010).","journal-title":"PLoS One"},{"key":"BFsrep42625_CR35","doi-asserted-by":"publisher","first-page":"e69717","DOI":"10.1371\/journal.pone.0069717","volume":"8","author":"MJ Sweet","year":"2013","unstructured":"Sweet, M. J., Bythell, J. C. & Nugues, M. M. Algae as reservoirs for coral pathogens. PLoS One 8, e69717, doi: 10.1371\/journal.pone.0069717 (2013).","journal-title":"PLoS One"},{"key":"BFsrep42625_CR36","doi-asserted-by":"publisher","first-page":"835","DOI":"10.1111\/j.1461-0248.2006.00937.x","volume":"9","author":"JE Smith","year":"2006","unstructured":"Smith, J. E. et al. Indirect effects of algae on coral: algae-mediated, microbe-induced coral mortality. Ecol. Lett. 9, 835\u2013845, doi: 10.1111\/j.1461-0248.2006.00937.x (2006).","journal-title":"Ecol. Lett."},{"key":"BFsrep42625_CR37","doi-asserted-by":"publisher","first-page":"e2393","DOI":"10.1371\/journal.pone.0002393","volume":"3","author":"M Sussman","year":"2008","unstructured":"Sussman, M., Willis, B. L., Victor, S. & Bourne, D. G. Coral pathogens identified for White Syndrome (WS) epizootics in the Indo-Pacific. PLoS One 3, e2393, doi: 10.1371\/journal.pone.0002393 (2008).","journal-title":"PLoS One"},{"key":"BFsrep42625_CR38","doi-asserted-by":"publisher","first-page":"2184","DOI":"10.1111\/j.1462-2920.2012.02746.x","volume":"14","author":"M Sweet","year":"2012","unstructured":"Sweet, M. & Bythell, J. Ciliate and bacterial communities associated with White Syndrome and Brown Band Disease in reef-building corals. Environ. Microbiol. 14, 2184\u20132199, doi: 10.1111\/j.1462-2920.2012.02746.x (2012).","journal-title":"Environ. Microbiol"},{"key":"BFsrep42625_CR39","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1038\/nrmicro732","volume":"1","author":"A Casadevall","year":"2003","unstructured":"Casadevall, A. & Pirofski, L.-A. The damage-response framework of microbial pathogenesis. Nat. Rev. Microbiol. 1, 17\u201324, doi: 10.1038\/nrmicro732 (2003).","journal-title":"Nat. Rev. Microbiol."},{"key":"BFsrep42625_CR40","doi-asserted-by":"publisher","first-page":"662","DOI":"10.1093\/icb\/icq061","volume":"50","author":"ME Mouchka","year":"2010","unstructured":"Mouchka, M. E., Hewson, I. & Harvell, C. D. Coral-associated bacterial assemblages: current knowledge and the potential for climate-driven impacts. Integr. Comp. Biol. 50, 662\u2013674, doi: 10.1093\/icb\/icq061 (2010).","journal-title":"Integr. Comp. Biol."},{"key":"BFsrep42625_CR41","doi-asserted-by":"publisher","first-page":"925","DOI":"10.1111\/1462-2920.12288","volume":"16","author":"S Egan","year":"2014","unstructured":"Egan, S., Fernandes, N. D., Kumar, V., Gardiner, M. & Thomas, T. Bacterial pathogens, virulence mechanism and host defence in marine macroalgae. Environ. Microbiol. 16, 925\u2013938, doi: 10.1111\/1462-2920.12288 (2014).","journal-title":"Environ. Microbiol."},{"key":"BFsrep42625_CR42","doi-asserted-by":"publisher","first-page":"140","DOI":"10.1111\/1365-2745.12028","volume":"101","author":"JR Svensson","year":"2013","unstructured":"Svensson, J. R., Nylund, G. M., Cervin, G., Toth, G. B. & Pavia, H. Novel chemical weapon of an exotic macroalga inhibits recruitment of native competitors in the invaded range. J. Ecol. 101, 140\u2013148, doi: 10.1111\/1365-2745.12028 (2013).","journal-title":"J. Ecol."},{"key":"BFsrep42625_CR43","doi-asserted-by":"publisher","first-page":"161","DOI":"10.3354\/meps09032","volume":"426","author":"VJ Paul","year":"2011","unstructured":"Paul, V. J. et al. Chemically mediated interactions between macroalgae Dictyota spp. and multiple life-history stages of the coral Porites astreoides . Mar. Ecol. Prog. Ser. 426, 161\u2013170, doi: 10.3354\/meps09032 (2011).","journal-title":"Mar. Ecol. Prog. Ser."},{"key":"BFsrep42625_CR44","doi-asserted-by":"publisher","first-page":"73","DOI":"10.3354\/meps282073","volume":"282","author":"R Mart\u00ed","year":"2004","unstructured":"Mart\u00ed, R., Uriz, M. J. & Turon, X. Seasonal and spatial variation of species toxicity in Mediterranean seaweed communities: correlation to biotic and abiotic factors. Mar. Ecol. Prog. Ser. 282, 73\u201385, doi: 10.3354\/meps282073 (2004).","journal-title":"Mar. Ecol. Prog. Ser."},{"key":"BFsrep42625_CR45","doi-asserted-by":"publisher","first-page":"87","DOI":"10.3354\/meps306087","volume":"306","author":"NA Paul","year":"2006","unstructured":"Paul, N. A., de Nys, R. & Steinberg, P. D. Chemical defence against bacteria in the red alga Asparagopsis armata: linking structure with function. Mar. Ecol. Prog. Ser. 306, 87\u2013101, doi: 10.3354\/meps306087 (2006).","journal-title":"Mar. Ecol. Prog. Ser."},{"key":"BFsrep42625_CR46","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3354\/meps323001","volume":"323","author":"NA Paul","year":"2006","unstructured":"Paul, N. A., de Nys, R. & Steinberg, P. D. Seaweed-herbivore interactions at a small scale: direct tests of feeding deterrence by filamentous algae. Mar. Ecol. Prog. Ser. 323, 1\u20139, doi: 10.3354\/meps323001 (2006).","journal-title":"Mar. Ecol. Prog. Ser."},{"key":"BFsrep42625_CR47","doi-asserted-by":"publisher","first-page":"1334","DOI":"10.1890\/07-0248.1","volume":"89","author":"A Verg\u00e9s","year":"2008","unstructured":"Verg\u00e9s, A., Paul, N. A. & Steinberg, P. D. Sex and life-history stage alter herbivore responses to a chemically defended red alga. Ecology 89, 1334\u20131343, doi: 10.1890\/07-0248.1 (2008).","journal-title":"Ecology"},{"key":"BFsrep42625_CR48","doi-asserted-by":"publisher","first-page":"403","DOI":"10.1007\/s00027-007-0889-y","volume":"69","author":"T Wiesemeier","year":"2007","unstructured":"Wiesemeier, T., Hay, M. & Pohnert, G. The potential role of wound-activated volatile release in the chemical defence of the brown alga Dictyota dichotoma: Blend recognition by marine herbivores. Aquat. Sci. 69, 403\u2013412 doi: 10.1007\/s00027-007-0889-y (2007).","journal-title":"Aquat. Sci."},{"key":"BFsrep42625_CR49","first-page":"91","volume":"42","author":"K Bouarab","year":"2001","unstructured":"Bouarab, K., Kloareg, B., Potin, P. & Correa, J. A. Ecological and biochemical aspects in algal infectious diseases. Cah. Biol. Mar. 42, 91\u2013100 (2001).","journal-title":"Cah. Biol. Mar."},{"key":"BFsrep42625_CR50","doi-asserted-by":"crossref","unstructured":"Jormalainen, V. & Honkanen, T. In Algal Chemical Ecology (ed. Charles D. Amsler ) Ch. 3, 57\u201389 (Springer Berlin Heidelberg, 2008).","DOI":"10.1007\/978-3-540-74181-7_3"},{"key":"BFsrep42625_CR51","doi-asserted-by":"publisher","first-page":"308","DOI":"10.1016\/s1369-5266(02)00273-x","volume":"5","author":"P Potin","year":"2002","unstructured":"Potin, P., Bouarab, K., Sala\u00fcn, J. P., Pohnert, G. & Kloareg, B. Biotic interactions of marine algae. Curr. Opin. Plant Biol. 5, 308\u2013317, doi: 10.1016\/s1369-5266(02)00273-x (2002).","journal-title":"Curr. Opin. Plant Biol."},{"key":"BFsrep42625_CR52","doi-asserted-by":"publisher","first-page":"2736","DOI":"10.1890\/12-0143.1","volume":"93","author":"S Enge","year":"2012","unstructured":"Enge, S., Nylund, G. M., Harder, T. & Pavia, H. An exotic chemical weapon explains low herbivore damage in an invasive alga. Ecology 93, 2736\u20132745, doi: 10.1890\/12-0143.1 (2012).","journal-title":"Ecology"},{"key":"BFsrep42625_CR53","unstructured":"Bianchi, C. N. In Biodiversity in Enclosed Seas and Artificial Marine Habitats Vol. 193 Developments in Hydrobiology (eds Relini, G. & Ryland, J. ) Ch. 1, 7\u201321 (Springer Netherlands, 2007)."},{"key":"BFsrep42625_CR54","doi-asserted-by":"publisher","first-page":"2285","DOI":"10.1111\/j.1365-294X.2007.03306.x","volume":"16","author":"N Andreakis","year":"2007","unstructured":"Andreakis, N., Procaccini, G., Maggs, C. & Kooistra, W. H. C. F. Phylogeography of the invasive seaweed Asparagopsis (Bonnemaisoniales, Rhodophyta) reveals cryptic diversity. Mol. Ecol. 16, 2285\u20132299, doi: 10.1111\/j.1365-294X.2007.03306.x (2007).","journal-title":"Mol. Ecol."},{"key":"BFsrep42625_CR55","doi-asserted-by":"publisher","first-page":"141","DOI":"10.1111\/jpy.12373","volume":"52","author":"N Andreakis","year":"2016","unstructured":"Andreakis, N., Costello, P., Zanolla, M., Saunders, G. W. & Mata, L. Endemic or introduced? Phylogeography of Asparagopsis (Florideophyceae) in Australia reveals multiple introductions and a new mitochondrial lineage. Journal of Phycology 52, 141\u2013147, doi: 10.1111\/jpy.12373 (2016).","journal-title":"Journal of Phycology"},{"key":"BFsrep42625_CR56","doi-asserted-by":"publisher","first-page":"79","DOI":"10.3354\/meps162079","volume":"162","author":"R de Nys","year":"1998","unstructured":"de Nys, R., Dworjanyn, S. A. & Steinberg, P. D. A new method for determining surface concentrations of marine natural products on seaweeds. Mar. Ecol. Prog. Ser. 162, 79\u201387, doi: 10.3354\/meps162079 (1998).","journal-title":"Mar. Ecol. Prog. Ser."},{"key":"BFsrep42625_CR57","unstructured":"Lane, D. J. 16S\/23S rRNA sequencing (Wiley, 1991)."},{"key":"BFsrep42625_CR58","doi-asserted-by":"publisher","first-page":"e56329","DOI":"10.1371\/journal.pone.0056329","volume":"8","author":"N Bodenhausen","year":"2013","unstructured":"Bodenhausen, N., Horton, M. W. & Bergelson, J. Bacterial Communities Associated with the Leaves and the Roots of Arabidopsis thaliana . PLoS One 8, e56329, doi: 10.1371\/journal.pone.0056329 (2013).","journal-title":"PLoS One"},{"key":"BFsrep42625_CR59","doi-asserted-by":"publisher","first-page":"335","DOI":"10.1038\/nmeth.f.303","volume":"7","author":"JG Caporaso","year":"2010","unstructured":"Caporaso, J. G. et al. QIIME allows analysis of high-throughput community sequencing data. Nat Methods 7, 335\u2013336, doi: 10.1038\/nmeth.f.303 (2010).","journal-title":"Nat Methods"},{"key":"BFsrep42625_CR60","doi-asserted-by":"publisher","first-page":"1655","DOI":"10.1098\/rspb.2011.2155","volume":"279","author":"KL Barott","year":"2012","unstructured":"Barott, K. L. et al. Microbial to reef scale interactions between the reef-building coral Montastraea annularis and benthic algae. Proc. R. Soc. B-Biol. Sci. 279, 1655\u20131664, doi: 10.1098\/rspb.2011.2155 (2012).","journal-title":"Proc. R. Soc. B-Biol. Sci"},{"key":"BFsrep42625_CR61","doi-asserted-by":"publisher","first-page":"2460","DOI":"10.1093\/bioinformatics\/btq461","volume":"26","author":"RC Edgar","year":"2010","unstructured":"Edgar, R. C. Search and clustering orders of magnitude faster than BLAST. Bioinformatics 26, 2460\u20132461, doi: 10.1093\/bioinformatics\/btq461 (2010).","journal-title":"Bioinformatics"},{"key":"BFsrep42625_CR62","doi-asserted-by":"publisher","first-page":"5261","DOI":"10.1128\/aem.00062-07","volume":"73","author":"Q Wang","year":"2007","unstructured":"Wang, Q., Garrity, G. M., Tiedje, J. M. & Cole, J. R. Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy. Appl Environ Microbiol 73, 5261\u20135267, doi: 10.1128\/aem.00062-07 (2007).","journal-title":"Appl Environ Microbiol"},{"key":"BFsrep42625_CR63","doi-asserted-by":"publisher","first-page":"610","DOI":"10.1038\/ismej.2011.139","volume":"6","author":"D McDonald","year":"2012","unstructured":"McDonald, D. et al. An improved Greengenes taxonomy with explicit ranks for ecological and evolutionary analyses of bacteria and archaea. ISME J. 6, 610\u2013618, doi: 10.1038\/ismej.2011.139 (2012).","journal-title":"ISME J."},{"key":"BFsrep42625_CR64","doi-asserted-by":"publisher","first-page":"1150","DOI":"10.1021\/np401094h","volume":"77","author":"S Greff","year":"2014","unstructured":"Greff, S. et al. Mahorones, highly brominated cyclopentenones from the red alga Asparagopsis taxiformis . J. Nat. Prod. 77, 1150\u20131155, doi: 10.1021\/np401094h (2014).","journal-title":"J. Nat. Prod."},{"key":"BFsrep42625_CR65","doi-asserted-by":"publisher","first-page":"779","DOI":"10.1021\/ac051437y","volume":"78","author":"CA Smith","year":"2006","unstructured":"Smith, C. A., Want, E. J., O\u2019Maille, G., Abagyan, R. & Siuzdak, G. XCMS: processing mass spectrometry data for metabolite profiling using nonlinear peak alignment, matching, and identification. Anal. Chem. 78, 779\u2013787, doi: 10.1021\/ac051437y (2006).","journal-title":"Anal. Chem."},{"key":"BFsrep42625_CR66","doi-asserted-by":"publisher","first-page":"5132","DOI":"10.1021\/pr900499r","volume":"8","author":"FM van der Kloet","year":"2009","unstructured":"van der Kloet, F. M., Bobeldijk, I., Verheij, E. R. & Jellema, R. H. Analytical error reduction using single point calibration for accurate and precise metabolomic phenotyping. J. Proteome Res. 8, 5132\u20135141, doi: 10.1021\/pr900499r (2009).","journal-title":"J. Proteome Res."},{"key":"BFsrep42625_CR67","unstructured":"Lawrence, M. A. ez: Easy analysis and visualization of factorial experiments., &lt;R package version 4.2-1. http:\/\/github.com\/mike-lawrence\/ez (2013)."},{"key":"BFsrep42625_CR68","unstructured":"R_Core_Team. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. http:\/\/www.R-project.org (2013)."},{"key":"BFsrep42625_CR69","doi-asserted-by":"publisher","first-page":"1","DOI":"10.18637\/jss.v022.i04","volume":"22","author":"S Dray","year":"2007","unstructured":"Dray, S. & Dufour, A. B. The ade4 package: implementing the duality diagram for ecologists. J. Stat. Softw. 22, 1\u201320 (2007).","journal-title":"J. Stat. Softw."},{"key":"BFsrep42625_CR70","unstructured":"Oksanen, J. et al. Vegan: community ecology package. R package version 2.3-0. URL http:\/\/CRAN.R-project.org\/package=vegan (2015)."}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/srep42625","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/srep42625.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/srep42625.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,12,23]],"date-time":"2022-12-23T20:12:27Z","timestamp":1671826347000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/srep42625"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,2,20]]},"references-count":70,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2017,5,12]]}},"alternative-id":["BFsrep42625"],"URL":"https:\/\/doi.org\/10.1038\/srep42625","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,2,20]]},"assertion":[{"value":"30 April 2016","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"13 January 2017","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"20 February 2017","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing financial interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"42625"}}