{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T17:47:33Z","timestamp":1782236853851,"version":"3.54.5"},"reference-count":47,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2011,1,14]],"date-time":"2011-01-14T00:00:00Z","timestamp":1294963200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,1,14]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The gastric mucosa is subject to continual bidirectional renewal by differentiation from stem and transit amplifying cells. It was the aim of this study to characterize the self-renewal of the human gastric mucosa and its two major types of glands in the fundus and antrum, respectively. Three characteristic regions (pit, proliferative, and lower neck regions) were isolated from fundic and antral units by the use of laser microdissection, and expression profiles concerning 15 marker genes were generated by RT-PCR analysis. The surface mucous cells (SMCs) of fundic and antral units differed in their expression of at least four secretory genes, i.e., gastric lipase, TFF3, FCGBP, and lysozyme. The maturation of mucous neck cells was shown to occur stepwise, first towards a mucous phenotype followed by a serous differentiation step. Also, a stepwise maturation of both the antral SMCs and antral gland cells was observed. Additionally, the presence of gastric lipase was also demonstrated for the first time in antral gland cells. In conclusion, the different expression profiles of SMCs of the fundic and antral units could be the basis for the different self-renewal rates of fundic and antral SMCs and could influence the spatial organization of the bacterial microbiota within the various parts of the gastric mucosa.<\/jats:p>\n                  <jats:p\/>","DOI":"10.1039\/c0mb00233j","type":"journal-article","created":{"date-parts":[[2011,1,14]],"date-time":"2011-01-14T10:57:57Z","timestamp":1295002677000},"page":"1105-1112","source":"Crossref","is-referenced-by-count":28,"title":["Self-renewal of the human gastric epithelium: new insights from expression profiling using laser microdissection"],"prefix":"10.1093","volume":"7","author":[{"given":"Irina","family":"Kouznetsova","sequence":"first","affiliation":[{"name":"Institute of Molecular Biology and Medicinal Chemistry, Otto-von-Guericke University a , Leipziger Str. 44, D-39120 Magdeburg Germany werner.hoffmann@med.ovgu.de"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Kalinski","sequence":"additional","affiliation":[{"name":"Institute of Pathology, Otto-von-Guericke University b , D-39120 Magdeburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"Meyer","sequence":"additional","affiliation":[{"name":"Department of Surgery, Otto-von-Guericke University c , D-39120 Magdeburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Werner","family":"Hoffmann","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology and Medicinal Chemistry, Otto-von-Guericke University a , Leipziger Str. 44, D-39120 Magdeburg Germany werner.hoffmann@med.ovgu.de"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2011,1,14]]},"reference":[{"key":"2026012913142321900_cit1","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1002\/jemt.1070310304","article-title":"Origin and migratory pathways of the eleven epithelial cell types present in the body of the mouse stomach","volume":"31","author":"Karam","year":"1995","journal-title":"Microsc. Res. Tech."},{"key":"2026012913142321900_cit2","doi-asserted-by":"crossref","first-page":"d286","DOI":"10.2741\/Karam","article-title":"Lineage commitment and maturation of epithelial cells in the gut","volume":"4","author":"Karam","year":"1999","journal-title":"Front. Biosci."},{"key":"2026012913142321900_cit3","doi-asserted-by":"crossref","first-page":"14819","DOI":"10.1073\/pnas.192574799","article-title":"Molecular characterization of mouse gastric epithelial progenitor cells","volume":"99","author":"Mills","year":"2002","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"2026012913142321900_cit4","doi-asserted-by":"crossref","first-page":"46138","DOI":"10.1074\/jbc.M308385200","article-title":"Molecular characterization of mouse gastric zymogenic cells","volume":"278","author":"Mills","year":"2003","journal-title":"J. Biol. Chem."},{"key":"2026012913142321900_cit5","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1634\/stemcells.21-3-322","article-title":"Defining epithelial cell progenitors in the human oxyntic mucosa","volume":"21","author":"Karam","year":"2003","journal-title":"Stem Cells"},{"key":"2026012913142321900_cit6","doi-asserted-by":"crossref","first-page":"3133","DOI":"10.2174\/092986708786848587","article-title":"Regeneration of the gastric mucosa and its glands from stem cells","volume":"15","author":"Hoffmann","year":"2008","journal-title":"Curr. Med. Chem."},{"key":"2026012913142321900_cit7","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.stem.2009.11.013","article-title":"Lgr5+ve stem cells drive self-renewal in the stomach and build long-lived gastric units in vitro","volume":"6","author":"Barker","year":"2010","journal-title":"Cell Stem Cell"},{"key":"2026012913142321900_cit8","doi-asserted-by":"crossref","first-page":"1681","DOI":"10.1053\/j.gastro.2010.03.002","article-title":"Leucine-rich repeat-containing G-protein-coupled receptors as markers of adult stem cells","volume":"138","author":"Barker","year":"2010","journal-title":"Gastroenterology"},{"key":"2026012913142321900_cit9","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1002\/(SICI)1096-9896(199902)187:3<331::AID-PATH241>3.0.CO;2-S","article-title":"The mucous neck cell in the human gastric corpus: a distinctive, functional cell lineage","volume":"187","author":"Hanby","year":"1999","journal-title":"J. Pathol."},{"key":"2026012913142321900_cit10","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1007\/s004180050011","article-title":"Coexistence of gland mucous cell-type mucin and lysozyme in gastric gland mucous cells","volume":"113","author":"Hidaka","year":"2000","journal-title":"Histochem. Cell Biol."},{"key":"2026012913142321900_cit11","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1007\/BF00215838","article-title":"Lysozyme localization in human gastric and duodenal epithelium","volume":"251","author":"Saito","year":"1988","journal-title":"Cell Tissue Res."},{"key":"2026012913142321900_cit12","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1111\/j.1699-0463.1992.tb03969.x","article-title":"Lysozyme localization in normal and diseased human gastric and colonic mucosa","volume":"100","author":"Santini","year":"1992","journal-title":"APMIS"},{"key":"2026012913142321900_cit13","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1016\/0046-8177(95)90219-8","article-title":"Proliferative markers in gastric carcinoma and organoid differentiation","volume":"26","author":"Fujimori","year":"1995","journal-title":"Hum. Pathol."},{"key":"2026012913142321900_cit14","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1016\/0016-5085(87)90005-9","article-title":"Subcellular localization of pepsinogen II in stomach and duodenum by the immunogold technique","volume":"92","author":"Cornaggia","year":"1987","journal-title":"Gastroenterology"},{"key":"2026012913142321900_cit15","first-page":"55","article-title":"Localization of pepsinogen (A and C) and cellular differentiation of pepsinogen-synthesizing cells in the human gastric mucosa","volume":"54","author":"Waalewijn","year":"1991","journal-title":"Eur. J. Cell Biol."},{"key":"2026012913142321900_cit16","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1007\/BF00508647","article-title":"Electron immunocytochemical localization of pepsinogen I (PgI) in chief cells, mucous-neck cells and transitional mucous-neck\/chief cells of the human fundic mucosa","volume":"85","author":"Cornaggia","year":"1986","journal-title":"Histochemistry"},{"key":"2026012913142321900_cit17","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1007\/BF00493829","article-title":"Immunocytolocalization of human gastric lipase in chief cells of the fundic mucosa","volume":"91","author":"Moreau","year":"1989","journal-title":"Histochemistry"},{"key":"2026012913142321900_cit18","doi-asserted-by":"crossref","first-page":"3671","DOI":"10.1074\/jbc.271.7.3671","article-title":"Diphtheria toxin-mediated ablation of parietal cells in the stomach of transgenic mice","volume":"271","author":"Li","year":"1996","journal-title":"J. Biol. Chem."},{"key":"2026012913142321900_cit19","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1590\/S0100-879X1998000200010","article-title":"Cell lineage relationship in the stomach of normal and genetically manipulated mice","volume":"31","author":"Karam","year":"1998","journal-title":"Braz. J. Med. Biol. Res."},{"key":"2026012913142321900_cit20","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1172\/JCI30111","article-title":"Inflammation, atrophy, and gastric cancer","volume":"117","author":"Fox","year":"2007","journal-title":"J. Clin. Invest."},{"key":"2026012913142321900_cit21","doi-asserted-by":"crossref","first-page":"2207","DOI":"10.1053\/j.gastro.2010.04.023","article-title":"Spasmolytic polypeptide-expressing metaplasia and intestinal metaplasia: time for reevaluation of metaplasias and the origins of gastric cancer","volume":"138","author":"Goldenring","year":"2010","journal-title":"Gastroenterology"},{"key":"2026012913142321900_cit22","doi-asserted-by":"crossref","first-page":"1804","DOI":"10.1053\/j.gastro.2007.03.040","article-title":"Potentiation of oxyntic atrophy-induced gastric metaplasia in amphiregulin-deficient mice","volume":"132","author":"Nam","year":"2007","journal-title":"Gastroenterology"},{"key":"2026012913142321900_cit23","doi-asserted-by":"crossref","first-page":"1288","DOI":"10.1053\/j.gastro.2008.12.037","article-title":"Amphiregulin-deficient mice develop spasmolytic polypeptide expressing metaplasia and intestinal metaplasia","volume":"136","author":"Nam","year":"2009","journal-title":"Gastroenterology"},{"key":"2026012913142321900_cit24","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1002\/(SICI)1097-0029(20000301)48:5<303::AID-JEMT7>3.0.CO;2-X","article-title":"Role of apoptosis in gastric epithelial turnover","volume":"48","author":"Herbay","year":"2000","journal-title":"Microsc. Res. Tech."},{"key":"2026012913142321900_cit25","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1007\/s00441-004-0854-1","article-title":"A gradient of TFF3 (trefoil factor family 3) peptide synthesis within the normal human gastric mucosa","volume":"316","author":"Kouznetsova","year":"2004","journal-title":"Cell Tissue Res."},{"key":"2026012913142321900_cit26","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1007\/s00441-006-0350-x","article-title":"Localization of TFF3 peptide in human esophageal submucosal glands and gastric cardia: differentiation of two types of pit cells along the rostro-caudal axis","volume":"328","author":"Kouznetsova","year":"2007","journal-title":"Cell Tissue Res."},{"key":"2026012913142321900_cit27","doi-asserted-by":"crossref","first-page":"899","DOI":"10.1159\/000110450","article-title":"Biosynthesis of gastrokine-2 in the human gastric mucosa: restricted spatial expression along the antral gland axis and differential interaction with TFF1, TFF2 and mucins","volume":"20","author":"Kouznetsova","year":"2007","journal-title":"Cell. Physiol. Biochem."},{"key":"2026012913142321900_cit28","doi-asserted-by":"crossref","first-page":"1126","DOI":"10.1096\/fasebj.14.9.1126","article-title":"Co-localization of TFF3 peptide and oxytocin in the human hypothalamus","volume":"14","author":"Jagla","year":"2000","journal-title":"FASEB J."},{"key":"2026012913142321900_cit29","first-page":"239","article-title":"Immunological characterization of digestive lipases","volume":"108","author":"de Caro","year":"1999","journal-title":"Methods Mol. Biol. (Totowa, N. J.)"},{"key":"2026012913142321900_cit30","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1007\/s004410050975","article-title":"Differential expression of the TFF-peptides xP1 and xP4 in the gastrointestinal tract of Xenopus laevis","volume":"291","author":"Jagla","year":"1998","journal-title":"Cell Tissue Res."},{"issue":"Suppl 101","key":"2026012913142321900_cit31","first-page":"87","article-title":"Rapid repair of injured gastric mucosa","volume":"19","author":"Ito","year":"1984","journal-title":"Scand. J. Gastroenterol."},{"key":"2026012913142321900_cit32","doi-asserted-by":"crossref","first-page":"1446","DOI":"10.1053\/j.gastro.2004.08.054","article-title":"Profiling of microdissected gastric epithelial cells reveals a cell type-specific response to Helicobacter pylori infection","volume":"127","author":"Mueller","year":"2004","journal-title":"Gastroenterology"},{"key":"2026012913142321900_cit33","doi-asserted-by":"crossref","first-page":"732","DOI":"10.1073\/pnas.0506655103","article-title":"Molecular analysis of the bacterial microbiota in the human stomach","volume":"103","author":"Bik","year":"2006","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"2026012913142321900_cit34","doi-asserted-by":"crossref","first-page":"993","DOI":"10.1021\/bi0518803","article-title":"How gastric lipase, an interfacial enzyme with a Ser-His-Asp catalytic triad, acts optimally at acidic pH","volume":"45","author":"Chahinian","year":"2006","journal-title":"Biochemistry"},{"key":"2026012913142321900_cit35","doi-asserted-by":"crossref","first-page":"851","DOI":"10.1007\/s00018-008-7546-z","article-title":"Gastric lipase: an extremophilic interfacial enzyme with medical applications","volume":"65","author":"Aloulou","year":"2008","journal-title":"Cell. Mol. Life Sci."},{"key":"2026012913142321900_cit36","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1146\/annurev.ph.57.030195.003025","article-title":"The hydrophobic barrier properties of gastrointestinal mucus","volume":"57","author":"Lichtenberger","year":"1995","journal-title":"Annu. Rev. Physiol."},{"key":"2026012913142321900_cit37","doi-asserted-by":"crossref","first-page":"1698","DOI":"10.1016\/0016-5085(93)91065-P","article-title":"Demonstration of a phospholipid-rich zone in the human gastric epithelium damaged by Helicobacter pylori","volume":"105","author":"Mauch","year":"1993","journal-title":"Gastroenterology"},{"key":"2026012913142321900_cit38","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1136\/gut.51.2.169","article-title":"Distribution and partial characterisation of IgG Fc binding protein in various mucin producing cells and body fluids","volume":"51","author":"Kobayashi","year":"2002","journal-title":"Gut"},{"key":"2026012913142321900_cit39","doi-asserted-by":"crossref","first-page":"3108","DOI":"10.1021\/pr100020c","article-title":"Human intestinal TFF3 forms disulfide-linked heteromers with the mucus-associated FCGBP protein and is released by hydrogen sulfide","volume":"9","author":"Albert","year":"2010","journal-title":"J. Proteome Res."},{"key":"2026012913142321900_cit40","doi-asserted-by":"crossref","first-page":"3549","DOI":"10.1021\/pr9002504","article-title":"Proteomic analyses of the two mucus layers of the colon barrier reveal that their main component, the Muc2 mucin, is strongly bound to the Fcgbp protein","volume":"8","author":"Johansson","year":"2009","journal-title":"J. Proteome Res."},{"key":"2026012913142321900_cit41","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/j.bbagen.2005.07.008","article-title":"Processing of lysozyme at distinct loops by pepsin: a novel action for generating multiple antimicrobial peptide motifs in the newborn stomach","volume":"1726","author":"Ibrahim","year":"2005","journal-title":"Biochim. Biophys. Acta"},{"key":"2026012913142321900_cit42","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1002\/ar.1092360204","article-title":"Dynamics of epithelial cells in the corpus of the mouse stomach III. Inward migration of neck cells followed by progressive transformation into zymogenic cells","volume":"236","author":"Karam","year":"1993","journal-title":"Anat. Rec."},{"key":"2026012913142321900_cit43","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1007\/BF00266591","article-title":"Glucoconjugate distribution in the human fundic mucosa revealed by lectin- and glycoprotein-gold cytochemistry","volume":"95","author":"Madrid","year":"1990","journal-title":"Histochemistry"},{"key":"2026012913142321900_cit44","doi-asserted-by":"crossref","first-page":"137","DOI":"10.2535\/ofaj1936.71.2-3_137","article-title":"Immunohistochemical examination of the relationship between two types of mucous neck cell and the intermediate cells in the rat fundic gland","volume":"71","author":"Fujita","year":"1994","journal-title":"Okajimas Folia Anat. Jpn."},{"key":"2026012913142321900_cit45","doi-asserted-by":"crossref","first-page":"7967","DOI":"10.1021\/bi047287n","article-title":"Interaction between TFF1, a gastric tumor suppressor trefoil protein, and TFIZ1, a brichos domain-containing protein with homology to SP-C","volume":"44","author":"Westley","year":"2005","journal-title":"Biochemistry"},{"key":"2026012913142321900_cit46","doi-asserted-by":"crossref","first-page":"1460","DOI":"10.1016\/S0016-5085(88)80063-5","article-title":"Gastric lipase: localization in the human stomach","volume":"95","author":"Abrams","year":"1988","journal-title":"Gastroenterology"},{"key":"2026012913142321900_cit47","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/0167-4781(87)90083-2","article-title":"Molecular cloning of a human gastric lipase and expression of the enzyme in yeast","volume":"909","author":"Bodmer","year":"1987","journal-title":"Biochim. Biophys. Acta"}],"container-title":["Molecular BioSystems"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/molecular-omics\/article-pdf\/7\/4\/1105\/66184969\/c0mb00233j.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/molecular-omics\/article-pdf\/7\/4\/1105\/66184969\/c0mb00233j.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T18:21:39Z","timestamp":1769710899000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/molecular-omics\/article\/7\/4\/1105\/8370152"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,1,14]]},"references-count":47,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2011,1,14]]}},"URL":"https:\/\/doi.org\/10.1039\/c0mb00233j","relation":{},"ISSN":["1742-2051","1742-206X"],"issn-type":[{"value":"1742-2051","type":"electronic"},{"value":"1742-206X","type":"print"}],"subject":[],"published-other":{"date-parts":[[2011,4,1]]},"published":{"date-parts":[[2011,1,14]]}}}