{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T11:11:55Z","timestamp":1769771515573,"version":"3.49.0"},"reference-count":26,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2011,3,14]],"date-time":"2011-03-14T00:00:00Z","timestamp":1300060800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,3,14]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Nitronyl nitroxides are capable of preventing cells, tissues, and organs from radical-induced damage through scavenging NO\u02d9, \u02d9O2\u2212 and \u02d9OH. In order to explore the conversions of nitronyl nitroxides in biological systems with and without NO\u02d9, HPLC-MS aided PC12cell systems were developed, and the conversions of 2-(3\u2032-nitrophenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl -3-oxide (3-nitro-PTIO), 1-oxyl-2-(3\u2032-nitrophenyl)-4,4,5,5-tetramethylimidazoline (3-nitro-PTI), and 1-hydroxyl-2-(3\u2032-nitrophenyl)-4,4,5,5-tetramethylimidazoline (3-nitro-PTIH) were quantitatively monitored. In these systems 3-nitro-PTIO and 3-nitro-PTI were time-dependently converted to 3-nitro-PTIH, while no conversion of 3-nitro-PTIH was detected. Free radical NO\u02d9 donors (sodium nitroprusside, SNP) accelerated the conversions, but had no effect upon the conversion product. In the in vitro and in vivo assays the 3-nitro-PTIH treated cells and mice exhibited no toxic response.<\/jats:p>\n                  <jats:p\/>","DOI":"10.1039\/c1mb05037k","type":"journal-article","created":{"date-parts":[[2011,3,14]],"date-time":"2011-03-14T10:00:29Z","timestamp":1300096829000},"page":"1678-1683","source":"Crossref","is-referenced-by-count":0,"title":["HPLC-MS aided PC12cell systems: to quantitatively monitor the conversion of nitronyl nitroxide in biological systems with and without NO"],"prefix":"10.1093","volume":"7","author":[{"given":"Yuji","family":"Wang","sequence":"first","affiliation":[{"name":"College of Pharmaceutical Sciences, Capital Medical University a , Beijing 100069, PR China shiqipeng@163.com sqpeng@bjmu.edu.cn mingzhao@bjmu.edu.cn 86-10-8391-1528 86-10-8391-1535 86-10-8391-1528 86-10-8391-1535"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xueyun","family":"Jiang","sequence":"additional","affiliation":[{"name":"College of Pharmaceutical Sciences, Capital Medical University a , Beijing 100069, PR China shiqipeng@163.com sqpeng@bjmu.edu.cn mingzhao@bjmu.edu.cn 86-10-8391-1528 86-10-8391-1535 86-10-8391-1528 86-10-8391-1535"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiawang","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Pharmaceutical Sciences, Capital Medical University a , Beijing 100069, PR China shiqipeng@163.com sqpeng@bjmu.edu.cn mingzhao@bjmu.edu.cn 86-10-8391-1528 86-10-8391-1535 86-10-8391-1528 86-10-8391-1535"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ming","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Pharmaceutical Sciences, Capital Medical University a , Beijing 100069, PR China shiqipeng@163.com sqpeng@bjmu.edu.cn mingzhao@bjmu.edu.cn 86-10-8391-1528 86-10-8391-1535 86-10-8391-1528 86-10-8391-1535"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guifeng","family":"Kang","sequence":"additional","affiliation":[{"name":"College of Pharmaceutical Sciences, Capital Medical University a , Beijing 100069, PR China shiqipeng@163.com sqpeng@bjmu.edu.cn mingzhao@bjmu.edu.cn 86-10-8391-1528 86-10-8391-1535 86-10-8391-1528 86-10-8391-1535"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianhui","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Pharmaceutical Sciences, Capital Medical University a , Beijing 100069, PR China shiqipeng@163.com sqpeng@bjmu.edu.cn mingzhao@bjmu.edu.cn 86-10-8391-1528 86-10-8391-1535 86-10-8391-1528 86-10-8391-1535"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Peng","sequence":"additional","affiliation":[{"name":"College of Pharmaceutical Sciences, Capital Medical University a , Beijing 100069, PR China shiqipeng@163.com sqpeng@bjmu.edu.cn mingzhao@bjmu.edu.cn 86-10-8391-1528 86-10-8391-1535 86-10-8391-1528 86-10-8391-1535"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shiqi","family":"Peng","sequence":"additional","affiliation":[{"name":"College of Pharmaceutical Sciences, Capital Medical University a , Beijing 100069, PR China shiqipeng@163.com sqpeng@bjmu.edu.cn mingzhao@bjmu.edu.cn 86-10-8391-1528 86-10-8391-1535 86-10-8391-1528 86-10-8391-1535"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2011,3,14]]},"reference":[{"key":"2026012913153641700_cit1","doi-asserted-by":"crossref","first-page":"1264","DOI":"10.1016\/S0891-5849(02)00804-3","volume":"32","author":"Giasson","year":"2002","journal-title":"Free Radical Biol. 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