{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T18:55:12Z","timestamp":1790189712777,"version":"4.1.0"},"reference-count":31,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2012,4,16]],"date-time":"2012-04-16T00:00:00Z","timestamp":1334534400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,4,16]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>A rapid and sensitive gold-nanobioprobe based immunoassay format has been presented for the detection of capsular Vi polysaccharide of Salmonella enterica serovar Typhi (surface antigen) using anti-Vi antibodies. The Vi antigen was extracted from serovar Typhi cells, under the optimised growth conditions for its over-expression. Anti-Vi antibodies were produced and conjugated with gold nanoparticles (GNPs) of definite size (\u223c30 nm), which served as the nano-bioprobe in the detection system. A sandwich immunoassay was developed using nitrocellulose dot blot comb (8\/12 wells) membranes immobilized with anti-Salmonella antibodies at the optimal concentration (43 ng spot\u22121). The Vi antigen in the clinical isolates, spiked samples and also in the standard strain (serovar Typhi Ty2) was detected by measuring the colour intensity of GNPs and correlating it with the concentration of serovar Typhi in samples. Using this developed immunoassay technique Vi positive serovar Typhi strains could be detected with a sensitivity of up to 102 cells mL\u22121 in the clinical isolates as well as in the spiked samples. The developed immunoassay technique could be useful for the detection of typhoid fever and may be important from an epidemiological point of view.<\/jats:p>\n                  <jats:p\/>","DOI":"10.1039\/c2mb25048a","type":"journal-article","created":{"date-parts":[[2012,4,16]],"date-time":"2012-04-16T06:30:33Z","timestamp":1334557833000},"page":"1853-1860","source":"Crossref","is-referenced-by-count":27,"title":["A gold nanoparticles based immuno-bioprobe for detection of Vi capsular polysaccharide of\n                    <i>Salmonella enterica<\/i>\n                    serovar Typhi"],"prefix":"10.1093","volume":"8","author":[{"given":"Satish Kumar","family":"Pandey","sequence":"first","affiliation":[{"name":"Institute of Microbial Technology b , Sector 39 A, Chandigarh 160036 India"},{"name":"Department of Microbiology, Basic Medical Sciences Block, Panjab University a , Sector-14, Chandigarh 160014 India \u00a0 rishipraveen@yahoo.com \u00a0 +91-172-2541770 \u00a0 +91-172-2534146"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. Raman","family":"Suri","sequence":"additional","affiliation":[{"name":"Institute of Microbial Technology b , Sector 39 A, Chandigarh 160036 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Chaudhry","sequence":"additional","affiliation":[{"name":"Venus Remedies Ltd. c , Baddi HP, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R P","family":"Tiwari","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Basic Medical Sciences Block, Panjab University a , Sector-14, Chandigarh 160014 India \u00a0 rishipraveen@yahoo.com \u00a0 +91-172-2541770 \u00a0 +91-172-2534146"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Praveen","family":"Rishi","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Basic Medical Sciences Block, Panjab University a , Sector-14, Chandigarh 160014 India \u00a0 rishipraveen@yahoo.com \u00a0 +91-172-2541770 \u00a0 +91-172-2534146"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2012,4,16]]},"reference":[{"key":"2026012913054612700_cit1","doi-asserted-by":"crossref","first-page":"1770","DOI":"10.1056\/NEJMra020201","volume":"347","author":"Parry","year":"2002","journal-title":"N. Engl. J. Med."},{"key":"2026012913054612700_cit2","doi-asserted-by":"crossref","first-page":"753","DOI":"10.3855\/jidc.606","volume":"3","author":"Ghenghesh","year":"2009","journal-title":"J. Infect. Dev. Countries"},{"key":"2026012913054612700_cit3","doi-asserted-by":"crossref","first-page":"114","DOI":"10.4269\/ajtmh.2006.74.114","volume":"74","author":"Srikantiah","year":"2006","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"2026012913054612700_cit4","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/S0895-3988(08)60019-7","volume":"21","author":"Kumar","year":"2008","journal-title":"Biomed. Environ. Sci."},{"key":"2026012913054612700_cit5","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/j.aca.2004.10.015","volume":"531","author":"Kameswara","year":"2005","journal-title":"Anal. Chim. Acta"},{"key":"2026012913054612700_cit6","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1016\/j.talanta.2008.07.014","volume":"77","author":"Dungchai","year":"2008","journal-title":"Talanta"},{"key":"2026012913054612700_cit7","first-page":"159","volume":"13","author":"Chaicumpa","year":"1995","journal-title":"Asian Pac. J. Allergy Immunol."},{"key":"2026012913054612700_cit8","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/0022-1759(89)90394-3","volume":"119","author":"Stott","year":"1989","journal-title":"J. Immunol. Methods"},{"key":"2026012913054612700_cit9","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1083\/jcb.111.1.1","volume":"111","author":"Feldherr","year":"1990","journal-title":"J. Cell Biol."},{"key":"2026012913054612700_cit10","doi-asserted-by":"crossref","first-page":"4700","DOI":"10.1021\/ja0296935","volume":"125","author":"Tkachenko","year":"2003","journal-title":"J. Am. Chem. Soc."},{"key":"2026012913054612700_cit11","doi-asserted-by":"crossref","first-page":"943","DOI":"10.1007\/s00216-007-1768-z","volume":"391","author":"Baptista","year":"2008","journal-title":"Anal. Bioanal. Chem."},{"key":"2026012913054612700_cit12","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jqsrt.2009.07.012","volume":"111","author":"Khlebtsov","year":"2010","journal-title":"J. Quant. Spectrosc. Radiat. Transfer"},{"key":"2026012913054612700_cit13","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/j.jcis.2008.04.043","volume":"323","author":"Wangoo","year":"2008","journal-title":"J. Colloid Interface Sci."},{"key":"2026012913054612700_cit14","doi-asserted-by":"crossref","first-page":"7238","DOI":"10.1021\/jp057170o","volume":"110","author":"Jain","year":"2006","journal-title":"J. Phys. Chem. B"},{"key":"2026012913054612700_cit15","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.cca.2006.05.026","volume":"373","author":"Huang","year":"2006","journal-title":"Clin. Chim. Acta"},{"key":"2026012913054612700_cit16","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1002\/elan.200704011","volume":"20","author":"Idegami","year":"2008","journal-title":"Electroanalysis"},{"key":"2026012913054612700_cit17","doi-asserted-by":"crossref","first-page":"628","DOI":"10.1128\/am.24.4.628-633.1972","volume":"24","author":"Wong","year":"1972","journal-title":"J. Appl. Microbiol."},{"key":"2026012913054612700_cit18","first-page":"5293","volume":"15","author":"Corcionivoschi","year":"2010","journal-title":"Rom. Biotechnol. Lett."},{"key":"2026012913054612700_cit19","doi-asserted-by":"crossref","first-page":"2125","DOI":"10.1039\/c0an00810a","volume":"136","author":"Boro","year":"2011","journal-title":"Analyst"},{"key":"2026012913054612700_cit20","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1016\/j.bios.2009.07.018","volume":"25","author":"Gandhi","year":"2009","journal-title":"Biosens. Bioelectron."},{"key":"2026012913054612700_cit21","doi-asserted-by":"crossref","first-page":"3084","DOI":"10.1128\/iai.61.7.3084-3089.1993","volume":"61","author":"Tartera","year":"1993","journal-title":"Infect. Immun."},{"key":"2026012913054612700_cit22","doi-asserted-by":"crossref","first-page":"835","DOI":"10.1046\/j.1365-2958.1998.00976.x","volume":"29","author":"Arricau","year":"1998","journal-title":"Mol. Microbiol."},{"key":"2026012913054612700_cit23","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1111\/j.1348-0421.2001.tb01283.x","volume":"45","author":"Zhao","year":"2001","journal-title":"Microbiol. Immunol."},{"key":"2026012913054612700_cit24","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1093\/infdis\/150.3.436","volume":"150","author":"Robbins","year":"1984","journal-title":"J. Infect. Dis."},{"key":"2026012913054612700_cit25","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1111\/j.1365-2958.1996.tb02664.x","volume":"22","author":"Coynault","year":"1996","journal-title":"Mol. Microbiol."},{"key":"2026012913054612700_cit26","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1146\/annurev.mi.48.100194.000413","volume":"48","author":"Lowen","year":"1994","journal-title":"Annu. Rev. Microbiol."},{"key":"2026012913054612700_cit27","doi-asserted-by":"crossref","first-page":"1382","DOI":"10.1128\/IAI.00888-06","volume":"75","author":"Santander","year":"2007","journal-title":"Infect. Immun."},{"key":"2026012913054612700_cit28","doi-asserted-by":"crossref","first-page":"3984","DOI":"10.1128\/iai.62.9.3984-3993.1994","volume":"62","author":"Pickard","year":"1994","journal-title":"Infect. Immun."},{"key":"2026012913054612700_cit29","doi-asserted-by":"crossref","first-page":"8163","DOI":"10.1021\/bi060446d","volume":"45","author":"Zhang","year":"2006","journal-title":"Biochemistry"},{"key":"2026012913054612700_cit30","doi-asserted-by":"crossref","first-page":"872","DOI":"10.1128\/jcm.18.4.872-876.1983","volume":"18","author":"N Taylor","year":"1983","journal-title":"J. Clin. Microbiol."},{"key":"2026012913054612700_cit31","doi-asserted-by":"crossref","first-page":"830","DOI":"10.1128\/IAI.00961-10","volume":"79","author":"Wilson","year":"2011","journal-title":"Infect. Immun."}],"container-title":["Molecular BioSystems"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/molecular-omics\/article-pdf\/8\/7\/1853\/66163931\/c2mb25048a.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/molecular-omics\/article-pdf\/8\/7\/1853\/66163931\/c2mb25048a.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T18:07:59Z","timestamp":1769710079000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/molecular-omics\/article\/8\/7\/1853\/8346495"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,4,16]]},"references-count":31,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2012,4,16]]}},"URL":"https:\/\/doi.org\/10.1039\/c2mb25048a","relation":{},"ISSN":["1742-2051","1742-206X"],"issn-type":[{"value":"1742-2051","type":"electronic"},{"value":"1742-206X","type":"print"}],"subject":[],"published-other":{"date-parts":[[2012,7,1]]},"published":{"date-parts":[[2012,4,16]]}}}