{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T00:13:52Z","timestamp":1789949632697,"version":"4.0.1"},"reference-count":44,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2012,6,1]],"date-time":"2012-06-01T00:00:00Z","timestamp":1338508800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Down syndrome is one of the most frequent chromosomal disorders, with a prevalence of approximately 1\/500 to 1\/800, depending on the maternal age distribution of the pregnant population. However, few reliable protein biomarkers have been used in the diagnosis of this disease. Recent progress in quantitative proteomics has offered opportunities to discover biomarkers for tracking the progression and for understanding the molecular mechanisms of Down syndrome. In the present study, placental samples were analyzed by fluorescence two-dimensional differential gel electrophoresis (2D-DIGE) and differentially expressed proteins were identified by matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). In total, 101 proteins have been firmly identified representing 80 unique gene products. These proteins mainly function in cytoskeleton structure and regulation (such as vimentin and Profilin-1). Additionally, our quantitative proteomics approach has identified numerous previously reported Down syndrome markers, such as myelin protein. Here we present several Down syndrome biomarkers including galectin-1, ataxin-3 and sprouty-related EVH1 domain-containing protein 2 (SPRED2), which have not been reported elsewhere and may be associated with the progression and development of the disease. In summary, we report a comprehensive placenta-based proteomics approach for the identification of potential biomarkers for Down syndrome, in which serum amyloid P-component (APCS) and ataxin-3 have been shown to be up-regulated in the maternal peripheral plasma of Down syndrome cases. The potential of utilizing these markers for the prognosis and screening of Down syndrome warrants further investigation.<\/jats:p>\n                  <jats:p\/>","DOI":"10.1039\/c2mb25081k","type":"journal-article","created":{"date-parts":[[2012,6,2]],"date-time":"2012-06-02T04:42:21Z","timestamp":1338612141000},"page":"2360-2372","source":"Crossref","is-referenced-by-count":11,"title":["Placenta proteome analysis from Down syndrome pregnancies for biomarker discovery"],"prefix":"10.1093","volume":"8","author":[{"given":"Chih-Ping","family":"Chen","sequence":"first","affiliation":[{"name":"School of Chinese Medicine, College of Chinese Medicine, China Medical University d , Taichung, Taiwan"},{"name":"Department of Biotechnology, Asia University c , Taichung, Taiwan"},{"name":"Department of Medical Research, Mackay Memorial Hospital b , Taipei, Taiwan"},{"name":"Department of Obstetrics and Gynecology, Mackay Memorial Hospital a , Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"You-Hsuan","family":"Chen","sequence":"additional","affiliation":[{"name":"Institute of Bioinformatics and Structural Biology & Department of Medical Sciences, National Tsing Hua University e , No.101 Kuang-Fu Rd. Sec.2, Hsinchu, 30013, Taiwan \u00a0 hlchan@life.nthu.edu.tw \u00a0 886-3-5715934 \u00a0 886-3-5742476"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Schu-Rern","family":"Chern","sequence":"additional","affiliation":[{"name":"Department of Medical Research, Mackay Memorial Hospital b , Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shing-Jyh","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Nursing, Yuanpei University g , Hsinchu, Taiwan"},{"name":"Department of Obstetrics and Gynecology, MacKay Memorial Hospital, Hsinchu Branch f , Hsinchu, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Te-Lung","family":"Tsai","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Science and Biotechnology, Yuanpei University j , Hsinchu, Taiwan"},{"name":"Institute of Biochemistry and Biotechnology, Chung Shan Medical University i , Taichung, Taiwan"},{"name":"Department of Pathology & Laboratory Medicine, MacKay Memorial Hospital, Hsinchu Branch h , Hsinchu, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheng-Hsiang","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Medical Research, Mackay Memorial Hospital b , Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hsiu-Chuan","family":"Chou","sequence":"additional","affiliation":[{"name":"Department of Applied Science, National Hsinchu University of Education k , Hsinchu, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Wen","family":"Lo","sequence":"additional","affiliation":[{"name":"Department of Applied Science, National Hsinchu University of Education k , Hsinchu, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping-Chiang","family":"Lyu","sequence":"additional","affiliation":[{"name":"Institute of Bioinformatics and Structural Biology & Department of Medical Sciences, National Tsing Hua University e , No.101 Kuang-Fu Rd. Sec.2, Hsinchu, 30013, Taiwan \u00a0 hlchan@life.nthu.edu.tw \u00a0 886-3-5715934 \u00a0 886-3-5742476"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong-Lin","family":"Chan","sequence":"additional","affiliation":[{"name":"Institute of Bioinformatics and Structural Biology & Department of Medical Sciences, National Tsing Hua University e , No.101 Kuang-Fu Rd. Sec.2, Hsinchu, 30013, Taiwan \u00a0 hlchan@life.nthu.edu.tw \u00a0 886-3-5715934 \u00a0 886-3-5742476"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2012,6,1]]},"reference":[{"key":"2026012913055347100_cit1","doi-asserted-by":"crossref","first-page":"1044","DOI":"10.1016\/j.ajog.2004.06.050","volume":"191","author":"Egan","year":"2004","journal-title":"Am. J. Obstet. 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