{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T09:28:55Z","timestamp":1769765335274,"version":"3.49.0"},"reference-count":22,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2012,6,21]],"date-time":"2012-06-21T00:00:00Z","timestamp":1340236800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,6,21]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The accelerated non-enzymatic modification of proteins by Maillard reaction during prolonged hyperglycemia is a key player in the diabetes associated pathology. In addition, hypoxia has been implicated in the recent past as a modulating factor. Therefore we have examined the interaction of glycation modified human serum albumin (AGE-HSA) and deferoxamine (DFO) mimicked hypoxia on the expression of hypoxia inducible factor 1\u03b1 (HIF-1\u03b1), and the role of RAGE (receptor for AGE) signaling in up-regulation of HIF-1\u03b1. Expression of VEGF (a downstream target of HIF-1\u03b1) and sICAM-1 (inflammatory marker) was also detected. When HUVEC were subjected to hypoxia, highest expression of HIF-1\u03b1 was observed. When treated with AGE-HSA at two concentrations, higher expression was found vis-a-vis control, with 0.2 mg ml\u22121 than 2.0 mg ml\u22121 which was mediated in part by RAGE as determined by RAGE silencing. However, when the cells were exposed to a combination treatment of hypoxia and AGE-HSA, a biphasic effect at the two different concentrations was observed as compared to the individual treatments. VEGF was synergistically up-regulated by hypoxia and AGE-HSA. On the other hand sICAM-1 was up-regulated by AGE-HSA but down-regulated by hypoxia. These results show that AGE-HSA functions as a non-hypoxic factor which modulates the expression of HIF-1\u03b1 in a concentration dependent manner in the range studied. It can be concluded that glycated serum proteins may activate HIF-1\u03b1 independently in diabetes. Further, when both glycated proteins and hypoxic conditions are present, they act in opposition in regulation of HIF-1\u03b1.<\/jats:p>\n                  <jats:p\/>","DOI":"10.1039\/c2mb25156f","type":"journal-article","created":{"date-parts":[[2012,6,22]],"date-time":"2012-06-22T13:21:24Z","timestamp":1340371284000},"page":"2657-2663","source":"Crossref","is-referenced-by-count":14,"title":["Interaction of glycated protein and DFO mimicked hypoxia in cellular responses of HUVECs"],"prefix":"10.1093","volume":"8","author":[{"given":"Kanchan","family":"Bala","sequence":"first","affiliation":[{"name":"Centre for Biomedical Engineering, Indian Institute of Technology a , Hauz Khas, New Delhi 110 016 India nkarm@yahoo.com nkar@cbme.iitd.ernet.in +91 11 2658 2037 +91 11 2659 1151"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nivedita Karmakar","family":"Gohil","sequence":"additional","affiliation":[{"name":"Centre for Biomedical Engineering, Indian Institute of Technology a , Hauz Khas, New Delhi 110 016 India nkarm@yahoo.com nkar@cbme.iitd.ernet.in +91 11 2658 2037 +91 11 2659 1151"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2012,6,21]]},"reference":[{"key":"2026012913061299800_cit1","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1007\/s001250051591","volume":"44","author":"Sing","year":"2001","journal-title":"Diabetologia"},{"key":"2026012913061299800_cit2","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1016\/S0140-6736(98)07019-6","volume":"352","author":"UK Prospective Diabetes Study (UKPDS) Group","year":"1998","journal-title":"Lancet"},{"key":"2026012913061299800_cit3","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1126\/science.151.3707.209","volume":"151","author":"Gabay","year":"1966","journal-title":"Science"},{"key":"2026012913061299800_cit4","doi-asserted-by":"crossref","first-page":"801","DOI":"10.2337\/diab.42.6.801","article-title":"et al.","volume":"42","author":"Williamson","year":"1993","journal-title":"Diabetes"},{"key":"2026012913061299800_cit5","first-page":"2294","volume":"82","author":"Shah","year":"1997","journal-title":"J. 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