{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T07:56:36Z","timestamp":1778054196428,"version":"3.51.4"},"reference-count":45,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2012,6,26]],"date-time":"2012-06-26T00:00:00Z","timestamp":1340668800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,6,26]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Dupuytren's disease (DD) is an ill-defined fibroproliferative disorder affecting the palm of the hand, resulting in progressive and irreversible digital contracture. In view of the abnormal gene dysregulation found in DD, and its potential effect on metabolites at a functional level, we chose to examine the metabolic profile involved in DD. Using Fourier transform infrared (FT-IR) spectroscopy to generate metabolic fingerprints of cultured cells, we compared the profiles of DD cords and nodules (1) against the unaffected transverse palmar fascia (internal control), (2) against carpal ligamentous fascia (external control), and (3) against fibroblasts from fat surrounding the nodule and skin overlying the nodule (environmental control). We also determined the effects of serial passaging of the cells on DD fingerprints. Subsequently, gas chromatography-mass spectrometry (GC-MS) was employed for metabolic profiling in order to identify metabolites characteristic of the DD tissue phenotypes. We developed a robust metabolomic analysis procedure of DD using cultured fibroblasts derived from DD tissues. Our carefully controlled culture conditions, combined with assessment of metabolic phenotypes by FT-IR and GC-MS, enabled us to demonstrate metabolic differences between DD and unaffected transverse palmar fascia and between DD and healthy control tissue. In early passage (0\u20133) the metabolic differences were clear, but cells from subsequent passages (4\u20136) started to lose this distinction between diseased and non-diseased origin. The dysregulated metabolites we identified were leucine, phenylalanine, lysine, cysteine, aspartic acid, glycerol-3-phosphate and the vitamin precursor to coenzyme A. Early passage DD cells exhibit a clear metabolic profile, in which central metabolic pathways appear to be involved. Experimental conditions have been identified in which these DD data are reproducible. The experimental reproducibility will be useful in DD diagnostics and for DD systems biology.<\/jats:p>\n                  <jats:p\/>","DOI":"10.1039\/c2mb25173f","type":"journal-article","created":{"date-parts":[[2012,6,26]],"date-time":"2012-06-26T08:57:50Z","timestamp":1340701070000},"page":"2274-2288","source":"Crossref","is-referenced-by-count":17,"title":["Dupuytren's disease metabolite analyses reveals alterations following initial short-term fibroblast culturing"],"prefix":"10.1093","volume":"8","author":[{"given":"Samrina","family":"Rehman","sequence":"first","affiliation":[{"name":"Manchester Centre for Integrative Systems Biology, Manchester Institute of Biotechnology, University of Manchester b , Manchester, M1 7DN, UK \u00a0 Roy.Goodacre@manchester.ac.uk"},{"name":"Doctoral Training Centre ISBML, The Manchester Centre for Integrative Systems Biology, Manchester Institute of Biotechnology, University of Manchester a , Manchester, M1 7DN, UK \u00a0 Samrina.Rehman@manchester.ac.uk \u00a0 Hans.Westerhoff@manchester.ac.uk"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yun","family":"Xu","sequence":"additional","affiliation":[{"name":"School of Chemistry, Manchester Institute of Biotechnology, University of Manchester d , Manchester, M1 7DN, UK \u00a0 Yun.Xu-2@manchester.ac.uk"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Warwick B","family":"Dunn","sequence":"additional","affiliation":[{"name":"Centre for Advanced Discovery & Experimental Therapeutics (CADET), Central Manchester NHS Foundation Trust and School of Biomedicine, University of Manchester, Manchester Academic Health Sciences Centre f , York Place Oxford Road, Manchester, M13 9WL, UK \u00a0 Warwick.Dunn@manchester.ac.uk"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philip J R","family":"Day","sequence":"additional","affiliation":[{"name":"Quantitative Molecular Medicine Research, CIGMR, Manchester Institute of Biotechnology, University of Manchester c , Manchester, M1 7DN, UK \u00a0 Philip.J.Day@manchester.ac.uk"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans V","family":"Westerhoff","sequence":"additional","affiliation":[{"name":"Synthetic Systems Biology, Swammerdam Institute for Life Sciences, University of Amsterdam h , The Netherlands"},{"name":"Netherlands Institute for Systems Biology, VU University Amsterdam g , NL-1081 HV, The Netherlands"},{"name":"Manchester Centre for Integrative Systems Biology, Manchester Institute of Biotechnology, University of Manchester b , Manchester, M1 7DN, UK \u00a0 Roy.Goodacre@manchester.ac.uk"},{"name":"Doctoral Training Centre ISBML, The Manchester Centre for Integrative Systems Biology, Manchester Institute of Biotechnology, University of Manchester a , Manchester, M1 7DN, UK \u00a0 Samrina.Rehman@manchester.ac.uk \u00a0 Hans.Westerhoff@manchester.ac.uk"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Royston","family":"Goodacre","sequence":"additional","affiliation":[{"name":"School of Chemistry, Manchester Institute of Biotechnology, University of Manchester d , Manchester, M1 7DN, UK \u00a0 Yun.Xu-2@manchester.ac.uk"},{"name":"Manchester Centre for Integrative Systems Biology, Manchester Institute of Biotechnology, University of Manchester b , Manchester, M1 7DN, UK \u00a0 Roy.Goodacre@manchester.ac.uk"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ardeshir","family":"Bayat","sequence":"additional","affiliation":[{"name":"Plastic and Reconstructive Surgery Research, Manchester Institute of Biotechnology, University of Manchester e , Manchester, M1 7DN, UK \u00a0 Ardeshir.Bayat@manchester.ac.uk"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2012,6,26]]},"reference":[{"key":"2026012913060469500_cit1","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1186\/ar3438","article-title":"Dupuytren's: a systems biology disease","volume":"13","author":"Rehman","year":"2011","journal-title":"Arthritis Res. 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Rheumatol."},{"key":"2026012913060469500_cit4","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1097\/01.bth.0000186794.90431.a4","article-title":"A review of common practice in Dupuytren surgery","volume":"9","author":"Au-Yong","year":"2005","journal-title":"Techniques in Hand and Upper Extremity Surgery"},{"key":"2026012913060469500_cit5","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/S0749-0712(21)00436-4","article-title":"Cellular structure and biology of Dupuytren's disease","volume":"15","author":"Tomasek","year":"1999","journal-title":"Hand. Clin."},{"key":"2026012913060469500_cit6","first-page":"131","article-title":"Dupuytren's contracture: fibroblast contraction? An ultrastructural study","volume":"66","author":"Gabbiani","year":"1972","journal-title":"Am. J. 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