{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T04:54:59Z","timestamp":1789448099196,"version":"build-2803163510"},"reference-count":51,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2013,3,27]],"date-time":"2013-03-27T00:00:00Z","timestamp":1364342400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,3,27]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Systems metabolomics, the identification and quantification of cellular metabolites and their integration with genomics and proteomics data, promises valuable functional insights into cellular biology. However, technical constraints, sample complexity issues and the lack of suitable complementary quantitative data sets prevented accomplishing such studies in the past. Here, we present an integrative metabolomics study of the genome-reduced bacterium Mycoplasma pneumoniae. We experimentally analysed its metabolome using a cross-platform approach. We explain intracellular metabolite homeostasis by quantitatively integrating our results with the cellular inventory of proteins, DNA and other macromolecules, as well as with available building blocks from the growth medium. We calculated in vivo catalytic parameters of glycolytic enzymes, making use of measured reaction velocities, as well as enzyme and metabolite pool sizes. A quantitative, inter-species comparison of absolute and relative metabolite abundances indicated that metabolic pathways are regulated as functional units, thereby simplifying adaptive responses. Our analysis demonstrates the potential for new scientific insight by integrating different types of large-scale experimental data from a single biological source.<\/jats:p>\n                  <jats:p\/>","DOI":"10.1039\/c3mb70113a","type":"journal-article","created":{"date-parts":[[2013,3,27]],"date-time":"2013-03-27T11:11:37Z","timestamp":1364382697000},"page":"1743-1755","source":"Crossref","is-referenced-by-count":33,"title":["Large-scale metabolome analysis and quantitative integration with genomics and proteomics data in\n                    <i>Mycoplasma pneumoniae<\/i>"],"prefix":"10.1093","volume":"9","author":[{"given":"Tobias","family":"Maier","sequence":"first","affiliation":[{"name":"Universitat Pompeu Fabra (UPF) b , Barcelona, Spain"},{"name":"EMBL\/CRG Systems Biology Research Unit, Centre for Genomic Regulation (CRG) a , Dr Aiguader 88, 08003 Barcelona Spain tobias.maier@crg.es luis.serrano@crg.es"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josep","family":"Marcos","sequence":"additional","affiliation":[{"name":"Bio-analysis Group, Neuroscience Research Program, IMIM-Parc Salut Mar, Barcelona Biomedical Research Park d , Dr Aiguader 88, 08003 Barcelona Spain"},{"name":"Department of Experimental and Health Sciences, Pompeu Fabra University, Barcelona Biomedical Research Park c , Dr Aiguader 88, 08003 Barcelona Spain rgutierrez@imim.es"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judith A H","family":"Wodke","sequence":"additional","affiliation":[{"name":"Theoretical Biophysics, Humboldt-Universit\u00e4t zu Berlin e , Berlin, Germany"},{"name":"Universitat Pompeu Fabra (UPF) b , Barcelona, Spain"},{"name":"EMBL\/CRG Systems Biology Research Unit, Centre for Genomic Regulation (CRG) a , Dr Aiguader 88, 08003 Barcelona Spain tobias.maier@crg.es luis.serrano@crg.es"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernhard","family":"Paetzold","sequence":"additional","affiliation":[{"name":"Universitat Pompeu Fabra (UPF) b , Barcelona, Spain"},{"name":"EMBL\/CRG Systems Biology Research Unit, Centre for Genomic Regulation (CRG) a , Dr Aiguader 88, 08003 Barcelona Spain tobias.maier@crg.es luis.serrano@crg.es"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuel","family":"Liebeke","sequence":"additional","affiliation":[{"name":"Department of Surgery and Cancer, Imperial College London f , Sir Alexander Fleming Building, London SW7 2AZ UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo","family":"Guti\u00e9rrez-Gallego","sequence":"additional","affiliation":[{"name":"Bio-analysis Group, Neuroscience Research Program, IMIM-Parc Salut Mar, Barcelona Biomedical Research Park d , Dr Aiguader 88, 08003 Barcelona Spain"},{"name":"Department of Experimental and Health Sciences, Pompeu Fabra University, Barcelona Biomedical Research Park c , Dr Aiguader 88, 08003 Barcelona Spain rgutierrez@imim.es"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis","family":"Serrano","sequence":"additional","affiliation":[{"name":"Instituci\u00f3 Catalana de Recerca i Estudis Avan\u00e7ats (ICREA) g , Pg. Lluis Companys 23, 08010 Barcelona Spain"},{"name":"Universitat Pompeu Fabra (UPF) b , Barcelona, Spain"},{"name":"EMBL\/CRG Systems Biology Research Unit, Centre for Genomic Regulation (CRG) a , Dr Aiguader 88, 08003 Barcelona Spain tobias.maier@crg.es luis.serrano@crg.es"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2013,3,27]]},"reference":[{"key":"2026012913124266200_cit1","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1039\/B906712B","volume":"40","author":"Dunn","year":"2011","journal-title":"Chem. Soc. Rev."},{"key":"2026012913124266200_cit2","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1038\/73696","volume":"18","author":"Cornish-Bowden","year":"2000","journal-title":"Nat. Biotechnol."},{"key":"2026012913124266200_cit3","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1023\/A:1013713905833","volume":"48","author":"Fiehn","year":"2002","journal-title":"Plant Mol. 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