{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T20:49:00Z","timestamp":1789159740947,"version":"build-2803163510"},"reference-count":41,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2014,1,24]],"date-time":"2014-01-24T00:00:00Z","timestamp":1390521600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":["rsc.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2014,1,24]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Bacterial antibiotic resistance has become a worldwide challenge with the overuse and misuse of drugs. Several mechanisms for the resistance are revealed, but information regarding the bacterial global response to antibiotics is largely absent. In this study, we characterized the differential proteome of Escherichia coli K12 BW25113 in response to chlortetracycline stress using isobaric tags for relative and absolute quantitation labeling quantitative proteomics technology. A total of 723 proteins including 10\u2009763 peptides were identified with 184 decreasing and 147 increasing in abundance by liquid chromatography matrix assisted laser desorption ionization mass spectrometry. Most interestingly, crucial metabolic pathways such as the tricarboxylic acid cycle, pyruvate metabolism and glycolysis\/gluconeogenesis sharply fluctuated, while the ribosome protein complexes contributing to the translation process were generally elevated in chlortetracycline stress, which is known for a compensative tactic due to the action of chlortetracycline on the ribosome. Further antimicrobial susceptibility assays validated the role of differential proteins in metabolic pathways using genetically modified mutants of gene deletion of these differential proteins. Our study demonstrated that the down-regulation of metabolic pathways was a part of the global response and played an important role in the antibiotics resistance. These results indicate that reverting of these fluctuated pathways may become a novel strategy to combat antibiotic-resistant bacteria.<\/jats:p>\n                  <jats:p\/>","DOI":"10.1039\/c3mb70522f","type":"journal-article","created":{"date-parts":[[2014,1,25]],"date-time":"2014-01-25T04:32:43Z","timestamp":1390624363000},"page":"901-908","update-policy":"https:\/\/doi.org\/10.1039\/rsc_crossmark_policy","source":"Crossref","is-referenced-by-count":76,"title":["Fluctuation of multiple metabolic pathways is required for\n                    <i>Escherichia coli<\/i>\n                    in response to chlortetracycline stress"],"prefix":"10.1093","volume":"10","author":[{"given":"Xiangmin","family":"Lin","sequence":"first","affiliation":[{"name":"Key Laboratory of Crop Ecology and Molecular Physiology (Fujian Agriculture and Forestry University), Fujian Province University c , Fuzhou 35002, People's Republic of China"},{"name":"Center for Proteomics and Metabolomics, State Key Laboratory of Bio-Control, School of Life Sciences, Sun Yat-Sen University, University City b , Guangzhou 510006, People's Republic of China \u00a0 pxuanx@sysu.edu.cn \u00a0 wangpeng@xmu.edu.cn"},{"name":"Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring (School of Life Sciences, Fujian Agriculture and Forestry University) a , Fuzhou 35002, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liqun","family":"Kang","sequence":"additional","affiliation":[{"name":"Center for Proteomics and Metabolomics, State Key Laboratory of Bio-Control, School of Life Sciences, Sun Yat-Sen University, University City b , Guangzhou 510006, People's Republic of China \u00a0 pxuanx@sysu.edu.cn \u00a0 wangpeng@xmu.edu.cn"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Li","sequence":"additional","affiliation":[{"name":"Center for Proteomics and Metabolomics, State Key Laboratory of Bio-Control, School of Life Sciences, Sun Yat-Sen University, University City b , Guangzhou 510006, People's Republic of China \u00a0 pxuanx@sysu.edu.cn \u00a0 wangpeng@xmu.edu.cn"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuanxian","family":"Peng","sequence":"additional","affiliation":[{"name":"Center for Proteomics and Metabolomics, State Key Laboratory of Bio-Control, School of Life Sciences, Sun Yat-Sen University, University City b , Guangzhou 510006, People's Republic of China \u00a0 pxuanx@sysu.edu.cn \u00a0 wangpeng@xmu.edu.cn"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2014,1,24]]},"reference":[{"key":"2026010815075483000_cit1","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1038\/nrmicro2319","volume":"8","author":"Andersson","year":"2010","journal-title":"Nat. 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