{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T09:49:11Z","timestamp":1776764951497,"version":"3.51.2"},"reference-count":55,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2014,1,30]],"date-time":"2014-01-30T00:00:00Z","timestamp":1391040000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,1,30]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Selenium (Se) has received great attention in the last few years, as it is considered to be essential for human health (prevention of viral infections, heart diseases and ageing-related diseases). Se deficiency can be counteracted by the administration of selenium-enriched probiotics that are able to convert inorganic selenium into less toxic and more bio-available organic forms. This study was performed on Lactobacillus reuteri Lb2 BM DSM 16143, a probiotic LAB previously demonstrated to be able to fix Se into selenocysteines. The aim was to assess Se influence on its metabolism, by a 2-DE proteomic approach, on two different cellular districts: envelope-enriched and extracellular proteomes. While in the envelope-enriched fraction 15 differentially expressed proteins were identified, in the extracellular proteome no quantitative difference was detected. However, at a molecular level, we observed the insertion of Se into selenocysteine, exclusively under the stimulated conditions. The obtained results confirmed the possibility to use L. reuteri Lb2 BM DSM 16143 as a carrier of organic Se that can be easily released in the gut becoming available for the human host.<\/jats:p>\n                  <jats:p\/>","DOI":"10.1039\/c3mb70557a","type":"journal-article","created":{"date-parts":[[2014,1,30]],"date-time":"2014-01-30T08:30:11Z","timestamp":1391070611000},"page":"1272-1280","source":"Crossref","is-referenced-by-count":27,"title":["Selenium effects on the metabolism of a Se-metabolizing\n                    <i>Lactobacillus reuteri<\/i>\n                    : analysis of envelope-enriched and extracellular proteomes"],"prefix":"10.1093","volume":"10","author":[{"given":"E","family":"Mangiapane","sequence":"first","affiliation":[{"name":"Department of Life Sciences and Systems Biology, University of Turin a , Via Accademia Albertina 13 10123 Torino Italy \u00a0 erika.mangiapane@unito.it"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C","family":"Lamberti","sequence":"additional","affiliation":[{"name":"CNR ISPA, c\/o Bioindustry Park S. Fumero b , Via Ribes 5 10010 Colleretto Giacosa Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Pessione","sequence":"additional","affiliation":[{"name":"Department of Life Sciences and Systems Biology, University of Turin a , Via Accademia Albertina 13 10123 Torino Italy \u00a0 erika.mangiapane@unito.it"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E","family":"Galano","sequence":"additional","affiliation":[{"name":"Department of Chemical Sciences, University of Naples \u201cFederico II\u201d c , Via Cinthia 4 80125 Napoli Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Amoresano","sequence":"additional","affiliation":[{"name":"Department of Chemical Sciences, University of Naples \u201cFederico II\u201d c , Via Cinthia 4 80125 Napoli Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E","family":"Pessione","sequence":"additional","affiliation":[{"name":"Department of Life Sciences and Systems Biology, University of Turin a , Via Accademia Albertina 13 10123 Torino Italy \u00a0 erika.mangiapane@unito.it"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2014,1,30]]},"reference":[{"key":"2026010815082257300_cit1","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1128\/MMBR.68.2.207-233.2004","volume":"68","author":"Tjalsma","year":"2004","journal-title":"Microbiol. 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