{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,30]],"date-time":"2026-05-30T02:05:32Z","timestamp":1780106732915,"version":"3.54.0"},"reference-count":42,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2016,4,21]],"date-time":"2016-04-21T00:00:00Z","timestamp":1461196800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01GM106016-2"],"award-info":[{"award-number":["R01GM106016-2"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01GM106016EB015722"],"award-info":[{"award-number":["R01GM106016EB015722"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006377","name":"Purdue University","doi-asserted-by":"publisher","award":["206732"],"award-info":[{"award-number":["206732"]}],"id":[{"id":"10.13039\/100006377","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R01GM106016-2"],"award-info":[{"award-number":["R01GM106016-2"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R01GM106016EB015722"],"award-info":[{"award-number":["R01GM106016EB015722"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100006377","name":"Purdue University","doi-asserted-by":"crossref","award":["206732"],"award-info":[{"award-number":["206732"]}],"id":[{"id":"10.13039\/100006377","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["rsc.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2016,4,21]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The zebrafish Danio rerio is a model vertebrate organism for understanding biological mechanisms. Recent studies have explored using zebrafish as a model for lipid-related diseases, for in vivo fish bioassays, and for embryonic toxicity experiments. Mass spectrometry (MS) and MS imaging are established tools for lipid profiling and spatial mapping of biomolecules and offer rapid, sensitive, and simple analytical protocols for zebrafish analysis. When ambient ionization techniques are used, ions are generated in native environmental conditions, requiring neither sample preparation nor separation of molecules prior to MS. We used two direct MS techniques to describe the dynamics of the lipid profile during zebrafish embryonic development from 0 to 96 hours post-fertilization and to explore these analytical approaches as molecular diagnostic assays. Desorption electrospray ionization (DESI) MS imaging followed by nanoelectrospray (nESI) MS and tandem MS (MS\/MS) were used in positive and negative ion modes, allowing the detection of a large variety of phosphatidylglycerols, phosphatidylcholines, phosphatidylinositols, free fatty acids, triacylglycerols, ubiquinone, squalene, and other lipids, and revealed information on the spatial distributions of lipids within the embryo and on lipid molecular structure. Differences were observed in the relative ion abundances of free fatty acids, triacylglycerols, and ubiquinone \u2013 essentially localized to the yolk \u2013 across developmental stages, whereas no relevant differences were found in the distribution of complex membrane glycerophospholipids, indicating conserved lipid constitution. Embryos exposed to trichloroethylene for 72 hours exhibited an altered lipid profile, indicating the potential utility of this technique for testing the effects of environmental contaminants.<\/jats:p>\n                  <jats:p\/>","DOI":"10.1039\/c6mb00168h","type":"journal-article","created":{"date-parts":[[2016,4,21]],"date-time":"2016-04-21T06:05:10Z","timestamp":1461218710000},"page":"2069-2079","update-policy":"https:\/\/doi.org\/10.1039\/rsc_crossmark_policy","source":"Crossref","is-referenced-by-count":49,"title":["Lipid dynamics in zebrafish embryonic development observed by DESI-MS imaging and nanoelectrospray-MS"],"prefix":"10.1093","volume":"12","author":[{"given":"V","family":"Pirro","sequence":"first","affiliation":[{"name":"Department of Chemistry and Center for Analytical Instrumentation Development, Purdue University a , West Lafayette, IN, 47907, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S C","family":"Guffey","sequence":"additional","affiliation":[{"name":"Department of Forestry and Natural Resources, Purdue University b , West Lafayette, IN, USA \u00a0 guffey0@purdue.edu \u00a0 +1-765-496-6266"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M S","family":"Sep\u00falveda","sequence":"additional","affiliation":[{"name":"Department of Forestry and Natural Resources, Purdue University b , West Lafayette, IN, USA \u00a0 guffey0@purdue.edu \u00a0 +1-765-496-6266"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C T","family":"Mahapatra","sequence":"additional","affiliation":[{"name":"Department of Forestry and Natural Resources, Purdue University b , West Lafayette, IN, USA \u00a0 guffey0@purdue.edu \u00a0 +1-765-496-6266"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C R","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Metabolite Profiling Facility, Bindley Bioscience Center, Purdue University c , West Lafayette, IN, USA"},{"name":"Department of Chemistry and Center for Analytical Instrumentation Development, Purdue University a , West Lafayette, IN, 47907, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A K","family":"Jarmusch","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Center for Analytical Instrumentation Development, Purdue University a , West Lafayette, IN, 47907, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R G","family":"Cooks","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Center for Analytical Instrumentation Development, Purdue University a , West Lafayette, IN, 47907, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2016,4,21]]},"reference":[{"key":"2026010816031972000_cit1","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1007\/978-1-60761-849-2_16","volume":"691","author":"Truong","year":"2011","journal-title":"Methods Mol. 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R. Ferreira (ResearcherID)","URL":"http:\/\/www.researcherid.com\/rid\/F-4744-2012","name":"identifier","group":{"name":"author_identifiers","label":"IDENTIFIERS"}},{"value":"The Royal Society of Chemistry has an exclusive publication licence for this journal","name":"copyright_licence","group":{"name":"copyright_licensing","label":"COPYRIGHT AND LICENSING"}},{"value":"Single-blind","order":2,"name":"peer_review_method","group":{"name":"peer_review_method","label":"PEER REVIEW METHOD"}},{"value":"Received 3 March 2016; Accepted 20 April 2016;  Accepted Manuscript published 21 April 2016;  Advance Article published 27 April 2016;  Version of Record published 21 June 2016","order":1,"name":"history","group":{"name":"publication_history","label":"PUBLICATION HISTORY"}}]}}