{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T03:47:38Z","timestamp":1784260058236,"version":"3.55.0"},"reference-count":48,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2016,5,3]],"date-time":"2016-05-03T00:00:00Z","timestamp":1462233600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100004748","name":"Mashhad University of Medical Sciences","doi-asserted-by":"publisher","award":["910070"],"award-info":[{"award-number":["910070"]}],"id":[{"id":"10.13039\/501100004748","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004748","name":"Mashhad University of Medical Sciences","doi-asserted-by":"crossref","award":["910070"],"award-info":[{"award-number":["910070"]}],"id":[{"id":"10.13039\/501100004748","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["rsc.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2016,5,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Tuberculosis (TB) remains a major global health threat despite chemotherapy and Bacilli Calmette\u2013Gu\u00e9rin (BCG) vaccination. Therefore, a safer and more effective vaccine against TB is urgently needed. This study evaluated the immunogenicity of a recombinant fusion protein consisting of early secreted antigenic target protein 6 kDa (ESAT-6), culture filtrate protein 10 kDa (CFP-10) and the Fc-domain of mouse IgG2a as a novel subunit vaccine. The recombinant expression vectors (pPICZ\u03b1A-ESAT-6:CFP-10:Fc\u03b32a and pPICZ\u03b1A-ESAT-6:CFP-10:His) were transferred into Pichia pastoris. After SDS-PAGE and immunoblotting, the immunogenicity of the recombinant proteins was evaluated in mice. When both recombinant proteins (ESAT-6:CFP-10:Fc\u03b32a and ESAT-6:CFP-10:His) were used for vaccination, Th1-type cellular responses were induced producing high levels of IFN-\u03b3 and IL-12. However, the Fc-tagged recombinant protein induced more effective Th1-type cellular responses with a small increase in IL-4 as compared to the BCG and ESAT-6:CFP-10:His groups. Moreover, mice primed with BCG and then supplemented with ESAT-6:CFP-10:Fc\u03b32a produced the highest levels of IFN-\u03b3 and IL-12 in immunized groups. The findings indicate that when Fc\u03b32a is fused to the ESAT-6:CFP-10 complex, as a delivery vehicle, there could be an increase in the immunogenicity of this type of subunit vaccine. Therefore, additional investigations are necessary for the development of appropriate Fc-based tuberculosis vaccines.<\/jats:p>\n                  <jats:p\/>","DOI":"10.1039\/c6mb00174b","type":"journal-article","created":{"date-parts":[[2016,5,3]],"date-time":"2016-05-03T05:30:52Z","timestamp":1462253452000},"page":"2189-2201","update-policy":"https:\/\/doi.org\/10.1039\/rsc_crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Fc-based delivery system enhances immunogenicity of a tuberculosis subunit vaccine candidate consisting of the ESAT-6:CFP-10 complex"],"prefix":"10.1093","volume":"12","author":[{"given":"Hadi","family":"Farsiani","sequence":"first","affiliation":[{"name":"Antimicrobial Resistance Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences a , Mashhad, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arman","family":"Mosavat","sequence":"additional","affiliation":[{"name":"Antimicrobial Resistance Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences a , Mashhad, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saman","family":"Soleimanpour","sequence":"additional","affiliation":[{"name":"Antimicrobial Resistance Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences a , Mashhad, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hamid","family":"Sadeghian","sequence":"additional","affiliation":[{"name":"Department of Laboratory Sciences, School of Paramedical Sciences, Mashhad University of Medical Sciences b , Mashhad, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad Reza","family":"Akbari Eydgahi","sequence":"additional","affiliation":[{"name":"Biotechnology Research Center, Semnan University of Medical Sciences c , Semnan, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kiarash","family":"Ghazvini","sequence":"additional","affiliation":[{"name":"Antimicrobial Resistance Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences a , Mashhad, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mojtaba","family":"Sankian","sequence":"additional","affiliation":[{"name":"Immunobiochemistry Lab, Immunology Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences d , Mashhad, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ehsan","family":"Aryan","sequence":"additional","affiliation":[{"name":"Antimicrobial Resistance Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences a , Mashhad, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saeid Amel","family":"Jamehdar","sequence":"additional","affiliation":[{"name":"Antimicrobial Resistance Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences a , Mashhad, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seyed Abdolrahim","family":"Rezaee","sequence":"additional","affiliation":[{"name":"Inflammation and Inflammatory Diseases Research Center, Medical School, Mashhad University of Medical Sciences e , Azadi-Square Medical Campus, Mashhad Iran \u00a0 RezaeeR@mums.ac.ir \u00a0 +98 (513) 843 6626 \u00a0 +98 (513) 800 2376"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2016,5,3]]},"reference":[{"key":"2026010816024019100_cit1","doi-asserted-by":"crossref","first-page":"656","DOI":"10.1038\/nrmicro1211","volume":"3","author":"Andersen","year":"2005","journal-title":"Nat. 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