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The pathogenic spirochaete, Leptospira interrogans, is characterized by its ability to permeate human host tissues rapidly and colonize multiple organs in the host. In spite of the efforts taken to comprehend the pathophysiology of the pathogen and the heterogeneity posed by L. interrogans, the current knowledge on the mechanism of pathogenesis is modest. In an attempt to contribute towards the same, we demonstrate the use of an established structure-based protocol coupled with information on subcellular localization of proteins and their tissue-specificity, in recognizing a set of 49 biologically feasible interactions potentially mediated by proteins of L. interrogans in humans. We have also presented means to adjudge the physicochemical viability of the predicted host\u2013pathogen interactions, for selected cases, in terms of interaction energies and geometric shape complementarity of the interacting proteins. Comparative analyses of proteins of L. interrogans and the saprophytic spirochaete, Leptospira biflexa, and their predicted involvement in interactions with human hosts, aided in underpinning the functional relevance of leptospiral\u2013host protein\u2013protein interactions specific to L. interrogans as well as those specific to L. biflexa. Our study presents characteristics of the pathogenic L. interrogans that are predicted to facilitate its ability to persist in human hosts.<\/jats:p>\n                  <jats:p\/>","DOI":"10.1039\/c6mb00856a","type":"journal-article","created":{"date-parts":[[2017,3,3]],"date-time":"2017-03-03T05:54:48Z","timestamp":1488520488000},"page":"883-891","update-policy":"https:\/\/doi.org\/10.1039\/rsc_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Comparison of\n                    <i>Leptospira interrogans<\/i>\n                    and\n                    <i>Leptospira biflexa<\/i>\n                    genomes: analysis of potential leptospiral\u2013host interactions"],"prefix":"10.1093","volume":"13","author":[{"given":"Prachi","family":"Mehrotra","sequence":"first","affiliation":[{"name":"Molecular Biophysics Unit, Indian Institute of Science b , Bangalore 560012, India"},{"name":"Indian Institute of Science Mathematics Initiative, Indian Institute of Science a , Bangalore 560012, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8203-2783","authenticated-orcid":false,"given":"Gayatri","family":"Ramakrishnan","sequence":"additional","affiliation":[{"name":"Molecular Biophysics Unit, Indian Institute of Science b , Bangalore 560012, India"},{"name":"Indian Institute of Science Mathematics Initiative, Indian Institute of Science a , Bangalore 560012, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gunasekaran","family":"Dhandapani","sequence":"additional","affiliation":[{"name":"Regional Medical Research Centre (ICMR) c , Port Blair 744 101, Andaman and Nicobar Islands India \u00a0 madananmg@icmr.org.in \u00a0 +91 9434284576"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Narayanaswamy","family":"Srinivasan","sequence":"additional","affiliation":[{"name":"Molecular Biophysics Unit, Indian Institute of Science b , Bangalore 560012, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6566-6239","authenticated-orcid":false,"given":"Madathiparambil G","family":"Madanan","sequence":"additional","affiliation":[{"name":"Regional Medical Research Centre (ICMR) c , Port Blair 744 101, Andaman and Nicobar Islands India \u00a0 madananmg@icmr.org.in \u00a0 +91 9434284576"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2017,3,3]]},"reference":[{"key":"2026010816051588800_cit1","doi-asserted-by":"crossref","first-page":"e0003898","DOI":"10.1371\/journal.pntd.0003898","volume":"9","author":"Costa","year":"2015","journal-title":"PLoS Neglected Trop. 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