{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,5]],"date-time":"2026-07-05T19:35:02Z","timestamp":1783280102095,"version":"3.54.6"},"reference-count":52,"publisher":"Royal Society of Chemistry (RSC)","issue":"20","content-domain":{"domain":["rsc.org"],"crossmark-restriction":true},"short-container-title":["Org. Biomol. Chem."],"abstract":"<p>By using a fluoride-sensitive probe, we harnessed P\u2013F bond cleavage reaction to develop an assay to screen for nucleotide-handling phosphohydrolase inhibitors.<\/p>","DOI":"10.1039\/c6ob00492j","type":"journal-article","created":{"date-parts":[[2016,3,18]],"date-time":"2016-03-18T11:03:00Z","timestamp":1458298980000},"page":"4595-4604","update-policy":"https:\/\/doi.org\/10.1039\/rsc_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["A fluorescent HTS assay for phosphohydrolases based on nucleoside 5\u2032-fluorophosphates: its application in screening for inhibitors of mRNA decapping scavenger and PDE-I"],"prefix":"10.1039","volume":"14","author":[{"given":"M. R.","family":"Baranowski","sequence":"first","affiliation":[{"name":"Division of Biophysics"},{"name":"Institute of Experimental Physics"},{"name":"Faculty of Physics"},{"name":"University of Warsaw"},{"name":"02-089 Warsaw"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Nowicka","sequence":"additional","affiliation":[{"name":"Division of Biophysics"},{"name":"Institute of Experimental Physics"},{"name":"Faculty of Physics"},{"name":"University of Warsaw"},{"name":"02-089 Warsaw"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7633-788X","authenticated-orcid":false,"given":"J.","family":"Jemielity","sequence":"additional","affiliation":[{"name":"Centre of New Technologies"},{"name":"University of Warsaw"},{"name":"02-097 Warsaw"},{"name":"Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9174-7999","authenticated-orcid":false,"given":"J.","family":"Kowalska","sequence":"additional","affiliation":[{"name":"Division of Biophysics"},{"name":"Institute of Experimental Physics"},{"name":"Faculty of Physics"},{"name":"University of Warsaw"},{"name":"02-089 Warsaw"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"292","published-online":{"date-parts":[[2016]]},"reference":[{"key":"C6OB00492J-(cit1)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1038\/nature13085","volume":"509","author":"Idzko","year":"2014","journal-title":"Nature"},{"key":"C6OB00492J-(cit2)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1007\/s11302-012-9309-4","volume":"8","author":"Zimmermann","year":"2012","journal-title":"Purinergic Signalling"},{"key":"C6OB00492J-(cit3)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"2322","DOI":"10.1056\/NEJMra1205750","volume":"367","author":"Eltzschig","year":"2012","journal-title":"N. Engl. J. Med."},{"key":"C6OB00492J-(cit4)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/j.mib.2013.02.005","volume":"16","author":"Penades","year":"2013","journal-title":"Curr. Opin. Microbiol."},{"key":"C6OB00492J-(cit5)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1152\/physrev.00030.2010","volume":"91","author":"Francis","year":"2011","journal-title":"Physiol. Rev."},{"key":"C6OB00492J-(cit6)\/*[position()=1]","first-page":"1","author":"Rampazzo","year":"2015","journal-title":"Cancer Chemother. Pharmacol."},{"key":"C6OB00492J-(cit7)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"1588","DOI":"10.1039\/c3sc21916j","volume":"4","author":"Hacker","year":"2013","journal-title":"Chem. Sci."},{"key":"C6OB00492J-(cit8)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"7262","DOI":"10.1039\/C4CC02422B","volume":"50","author":"Hacker","year":"2014","journal-title":"Chem. Commun."},{"key":"C6OB00492J-(cit9)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"2544","DOI":"10.1021\/acschembio.5b00459","volume":"10","author":"Hacker","year":"2015","journal-title":"ACS Chem. Biol."},{"key":"C6OB00492J-(cit10)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"8298","DOI":"10.1039\/c3ob41751d","volume":"11","author":"Hardt","year":"2013","journal-title":"Org. Biomol. Chem."},{"key":"C6OB00492J-(cit11)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1080\/07328319808005208","volume":"17","author":"Bollmark","year":"1998","journal-title":"Nucleosides Nucleotides"},{"key":"C6OB00492J-(cit12)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"1464","DOI":"10.1016\/j.tetlet.2014.01.051","volume":"55","author":"Aldersley","year":"2014","journal-title":"Tetrahedron Lett."},{"key":"C6OB00492J-(cit13)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"3982","DOI":"10.1021\/acs.joc.5b00337","volume":"80","author":"Baranowski","year":"2015","journal-title":"J. Org. Chem."},{"key":"C6OB00492J-(cit14)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"5272","DOI":"10.1016\/j.tetlet.2015.07.036","volume":"56","author":"Aldersley","year":"2015","journal-title":"Tetrahedron Lett."},{"key":"C6OB00492J-(cit15)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"3152","DOI":"10.1016\/j.febslet.2008.07.060","volume":"582","author":"Guranowski","year":"2008","journal-title":"FEBS Lett."},{"key":"C6OB00492J-(cit16)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"888","DOI":"10.1039\/b9nj00660e","volume":"34","author":"Guranowski","year":"2010","journal-title":"New J. Chem."},{"key":"C6OB00492J-(cit17)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"2115","DOI":"10.1039\/a906659d","author":"Misiura","year":"1999","journal-title":"Chem. Commun."},{"key":"C6OB00492J-(cit18)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/S0003-2670(01)01131-X","volume":"442","author":"Simonian","year":"2001","journal-title":"Anal. Chim. Acta"},{"key":"C6OB00492J-(cit19)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"2809","DOI":"10.1039\/b902069a","author":"Cametti","year":"2009","journal-title":"Chem. Commun."},{"key":"C6OB00492J-(cit20)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1007\/s10895-006-0140-6","volume":"17","author":"Yang","year":"2007","journal-title":"J. Fluoresc."},{"key":"C6OB00492J-(cit21)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"1559","DOI":"10.1002\/cbic.201200256","volume":"13","author":"Rydzik","year":"2012","journal-title":"ChemBioChem"},{"key":"C6OB00492J-(cit22)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"1765","DOI":"10.1039\/C4SC00020J","volume":"5","author":"Rydzik","year":"2014","journal-title":"Chem. Sci."},{"key":"C6OB00492J-(cit23)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"2494","DOI":"10.1039\/C2CC37270C","volume":"49","author":"Ke","year":"2013","journal-title":"Chem. Commun."},{"key":"C6OB00492J-(cit24)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"5768","DOI":"10.1039\/c3tb21041c","volume":"1","author":"Sivaraman","year":"2013","journal-title":"J. Mater. Chem. B"},{"key":"C6OB00492J-(cit25)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"4735","DOI":"10.1039\/b908745a","author":"Kim","year":"2009","journal-title":"Chem. Commun."},{"key":"C6OB00492J-(cit26)\/*[position()=1]","volume-title":"Histidine Triad (HIT) Superfamily","author":"Brenner","year":"2014","unstructured":"C.\n            Brenner\n          , Histidine Triad (HIT) Superfamily, eLS, 2014"},{"key":"C6OB00492J-(cit27)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"4699","DOI":"10.1093\/emboj\/cdf448","volume":"21","author":"Liu","year":"2002","journal-title":"EMBO J."},{"key":"C6OB00492J-(cit28)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"16688","DOI":"10.1038\/srep16688","volume":"5","author":"Meziane","year":"2015","journal-title":"Sci. Rep."},{"key":"C6OB00492J-(cit29)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"1132","DOI":"10.1261\/rna.1008208","volume":"14","author":"Shen","year":"2008","journal-title":"RNA"},{"key":"C6OB00492J-(cit30)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"1306","DOI":"10.1261\/rna.051573.115","volume":"21","author":"Zhou","year":"2015","journal-title":"RNA"},{"key":"C6OB00492J-(cit31)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1021\/cb800120t","volume":"3","author":"Singh","year":"2008","journal-title":"ACS Chem. Biol."},{"key":"C6OB00492J-(cit32)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"4084","DOI":"10.1093\/hmg\/ddt258","volume":"22","author":"Gogliotti","year":"2013","journal-title":"Hum. Mol. Genet."},{"key":"C6OB00492J-(cit33)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"4074","DOI":"10.1093\/hmg\/ddt257","volume":"22","author":"Van Meerbeke","year":"2013","journal-title":"Hum. Mol. Genet."},{"key":"C6OB00492J-(cit34)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"3003","DOI":"10.1111\/j.1742-4658.2010.07709.x","volume":"277","author":"Wypijewska","year":"2010","journal-title":"FEBS J."},{"key":"C6OB00492J-(cit35)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"8003","DOI":"10.1021\/bi300781g","volume":"51","author":"Wypijewska","year":"2012","journal-title":"Biochemistry"},{"key":"C6OB00492J-(cit36)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"1094","DOI":"10.1021\/cb5009475","volume":"10","author":"Hett","year":"2015","journal-title":"ACS Chem. Biol."},{"key":"C6OB00492J-(cit37)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1080\/10826068.2011.575319","volume":"41","author":"Al-Saleh","year":"2011","journal-title":"Prep. Biochem. Biotechnol."},{"key":"C6OB00492J-(cit38)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.snb.2014.03.054","volume":"199","author":"Cheng","year":"2014","journal-title":"Sens. Actuators, A"},{"key":"C6OB00492J-(cit39)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"612","DOI":"10.1039\/C5OB01541C","volume":"14","author":"Yevglevskis","year":"2016","journal-title":"Org. Biomol. Chem."},{"key":"C6OB00492J-(cit40)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1177\/108705719900400206","volume":"4","author":"Zhang","year":"1999","journal-title":"J. Biomol. Screening"},{"key":"C6OB00492J-(cit41)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"10245","DOI":"10.1093\/nar\/gku757","volume":"42","author":"Kowalska","year":"2014","journal-title":"Nucleic Acids Res."},{"key":"C6OB00492J-(cit42)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"1699","DOI":"10.1016\/j.bmc.2012.01.013","volume":"20","author":"Rydzik","year":"2012","journal-title":"Bioorg. Med. Chem."},{"key":"C6OB00492J-(cit43)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"3223","DOI":"10.1016\/j.bmc.2005.12.045","volume":"14","author":"Kalek","year":"2006","journal-title":"Bioorg. Med. Chem."},{"key":"C6OB00492J-(cit44)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"1119","DOI":"10.1261\/rna.990208","volume":"14","author":"Kowalska","year":"2008","journal-title":"RNA"},{"key":"C6OB00492J-(cit45)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"626","DOI":"10.1016\/j.bbabio.2011.02.001","volume":"1807","author":"Martin","year":"2011","journal-title":"Biochim. Biophys. Acta, Bioenerg."},{"key":"C6OB00492J-(cit46)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1007\/s00432-009-0751-9","volume":"136","author":"Hassan","year":"2010","journal-title":"J. Cancer Res. Clin. Oncol."},{"key":"C6OB00492J-(cit47)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"11529","DOI":"10.1021\/bi961415t","volume":"35","author":"Barnes","year":"1996","journal-title":"Biochemistry"},{"key":"C6OB00492J-(cit48)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1038\/nature12824","volume":"506","author":"Tumbale","year":"2014","journal-title":"Nature"},{"key":"C6OB00492J-(cit49)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1038\/nature05164","volume":"443","author":"Ahel","year":"2006","journal-title":"Nature"},{"key":"C6OB00492J-(cit50)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"4915","DOI":"10.1002\/anie.201000790","volume":"49","author":"Hu","year":"2010","journal-title":"Angew. Chem., Int. Ed."},{"key":"C6OB00492J-(cit51)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"3109","DOI":"10.1021\/ol0711873","volume":"9","author":"Kim","year":"2007","journal-title":"Org. Lett."},{"key":"C6OB00492J-(cit52)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"4763","DOI":"10.1039\/b911347a","volume":"7","author":"Rydzik","year":"2009","journal-title":"Org. Biomol. Chem."}],"container-title":["Organic &amp; Biomolecular Chemistry"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/pubs.rsc.org\/en\/content\/articlepdf\/2016\/OB\/C6OB00492J","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,5]],"date-time":"2026-07-05T18:30:03Z","timestamp":1783276203000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.rsc.org\/ob\/article\/14\/20\/4595-4604\/530550"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016]]},"references-count":52,"journal-issue":{"issue":"20","published-print":{"date-parts":[[2016]]}},"URL":"https:\/\/doi.org\/10.1039\/c6ob00492j","relation":{},"ISSN":["1477-0520","1477-0539"],"issn-type":[{"value":"1477-0520","type":"print"},{"value":"1477-0539","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016]]},"assertion":[{"value":"This document is Similarity Check deposited","name":"similarity_check","explanation":{"URL":"https:\/\/www.crossref.org\/services\/similarity-check\/"},"group":{"name":"similarity_check","label":"SIMILARITY CHECK"}},{"value":"Supplementary Information","URL":"https:\/\/doi.org\/10.1039\/C6OB00492J","name":"related_data","group":{"name":"related_data","label":"RELATED DATA"}},{"value":"J. Jemielity (ORCID)","URL":"http:\/\/orcid.org\/0000-0001-7633-788X","name":"identifier","group":{"name":"author_identifiers","label":"IDENTIFIERS"}},{"value":"J. Kowalska (ORCID)","URL":"http:\/\/orcid.org\/0000-0002-9174-7999","name":"identifier","group":{"name":"author_identifiers","label":"IDENTIFIERS"}},{"value":"The Royal Society of Chemistry has an exclusive publication licence for this journal","name":"copyright_licence","group":{"name":"copyright_licensing","label":"COPYRIGHT AND LICENSING"}},{"value":"The accepted version of this article will be made freely available after a 12 month embargo period","name":"copyright_licence","label":"OPEN ACCESS","group":{"name":"copyright_licensing","label":"COPYRIGHT AND LICENSING"}},{"value":"Single-blind","order":2,"name":"peer_review_method","group":{"name":"peer_review_method","label":"PEER REVIEW METHOD"}},{"value":"Received 3 March 2016; Accepted 18 March 2016;  Accepted Manuscript published 18 March 2016;  Advance Article published 31 March 2016;  Version of Record published 18 May 2016","order":1,"name":"history","group":{"name":"publication_history","label":"PUBLICATION HISTORY"}}]}}