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Bioinformatic analysis of the polyketide synthase indicated that extension modules 7 and 8 contain A-type ketoreductase and dehydratase domains. These modules are therefore predicted to form cis double bonds. The deduced stereostructure of the 67\u2013121C macrolactone is identical to that experimentally determined for the partricin subgroup of aromatic heptaenes. Some of these polyenes are N-methylated on the aminoacetophenone moiety. The C. caeruleus AceS protein was shown to methylate 4-aminoacetophenone and esters of 4-aminobenzoate, but not 4-aminobenzoate. This suggests that the substrate specificity of AceS prevents it from interfering with folate biosynthesis. The methyltransferase should be valuable for chemoenzymatic alkylation of compounds that contain aminobenzoyl moieties.<\/jats:p>\n                  <jats:p\/>","DOI":"10.1039\/c7mb00112f","type":"journal-article","created":{"date-parts":[[2017,3,31]],"date-time":"2017-03-31T06:19:01Z","timestamp":1490941141000},"page":"866-873","update-policy":"https:\/\/doi.org\/10.1039\/rsc_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["New insights into polyene macrolide biosynthesis in\n                    <i>Couchioplanes caeruleus<\/i>"],"prefix":"10.1093","volume":"13","author":[{"given":"J","family":"Sheehan","sequence":"first","affiliation":[{"name":"School of Biomolecular and Biomedical Science, University College Dublin a , Belfield, Dublin 4, Ireland \u00a0 patrick.caffrey@ucd.ie"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2137-3338","authenticated-orcid":false,"given":"C D","family":"Murphy","sequence":"additional","affiliation":[{"name":"School of Biomolecular and Biomedical Science, University College Dublin a , Belfield, Dublin 4, Ireland \u00a0 patrick.caffrey@ucd.ie"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0132-2410","authenticated-orcid":false,"given":"P","family":"Caffrey","sequence":"additional","affiliation":[{"name":"School of Biomolecular and Biomedical Science, University College Dublin a , Belfield, Dublin 4, Ireland \u00a0 patrick.caffrey@ucd.ie"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2017,3,31]]},"reference":[{"key":"2026010816065687800_cit1","doi-asserted-by":"crossref","first-page":"3893","DOI":"10.1007\/s00253-016-7474-z","volume":"100","author":"Caffrey","year":"2016","journal-title":"Appl. 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