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Poly-N-acetyl-lactosamine (polyLacNAc) substituted N-oligosaccharides on melanoma cell surface glycoproteins promote lung specific metastasis via galectin-3 by facilitating their arrest and extravasation. This study reports the identification and characterization of galectin-3 interacting proteins using a combination of galectin-3 sepharose affinity and leucoagglutinating phytohemagglutinin (L-PHA) columns. A total of 83 proteins were identified as galectin-3 interacting glycoproteins, of which 35 were constituents of the L-PHA bound fraction, suggesting that these proteins carry polyLacNAc substituted \u03b21,6 branched N-glycans. The identities of some of these proteins, like LAMP-1, LAMP-3, basigin, embigin, and \u03b15 and \u03b21 Integrin, have been confirmed by western blotting, and functional relevance with respect to metastatic properties has been established.<\/jats:p>\n                  <jats:p\/>","DOI":"10.1039\/c7mb00260b","type":"journal-article","created":{"date-parts":[[2017,8,25]],"date-time":"2017-08-25T14:26:54Z","timestamp":1503671214000},"page":"2303-2309","update-policy":"https:\/\/doi.org\/10.1039\/rsc_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Mass spectrometry based identification of galectin-3 interacting proteins potentially involved in lung melanoma metastasis"],"prefix":"10.1093","volume":"13","author":[{"given":"Manohar C","family":"Dange","sequence":"first","affiliation":[{"name":"Advanced Centre for Treatment Research and Education in Cancer (ACTREC), Tata Memorial Centre a , Sector 22, Kharghar, Navi Mumbai, 410210, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hemangi S","family":"Bhonsle","sequence":"additional","affiliation":[{"name":"Proteomics Facility, Division of Biochemical Sciences, CSIR-National Chemical Laboratory b , Pune, 411008, India \u00a0 mj.kulkarni@ncl.res.in"},{"name":"Advanced Centre for Treatment Research and Education in Cancer (ACTREC), Tata Memorial Centre a , Sector 22, Kharghar, Navi Mumbai, 410210, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rashmi K","family":"Godbole","sequence":"additional","affiliation":[{"name":"Proteomics Facility, Division of Biochemical Sciences, CSIR-National Chemical Laboratory b , Pune, 411008, India \u00a0 mj.kulkarni@ncl.res.in"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shyam K","family":"More","sequence":"additional","affiliation":[{"name":"Advanced Centre for Treatment Research and Education in Cancer (ACTREC), Tata Memorial Centre a , Sector 22, Kharghar, Navi Mumbai, 410210, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanjay M","family":"Bane","sequence":"additional","affiliation":[{"name":"Advanced Centre for Treatment Research and Education in Cancer (ACTREC), Tata Memorial Centre a , Sector 22, Kharghar, Navi Mumbai, 410210, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3932-9092","authenticated-orcid":false,"given":"Mahesh J","family":"Kulkarni","sequence":"additional","affiliation":[{"name":"Proteomics Facility, Division of Biochemical Sciences, CSIR-National Chemical Laboratory b , Pune, 411008, India \u00a0 mj.kulkarni@ncl.res.in"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajiv D","family":"Kalraiya","sequence":"additional","affiliation":[{"name":"Advanced Centre for Treatment Research and Education in Cancer (ACTREC), Tata Memorial Centre a , Sector 22, Kharghar, Navi Mumbai, 410210, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2017,8,25]]},"reference":[{"key":"2026010816044905700_cit1","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/j.cell.2011.09.024","volume":"147","author":"Valastyan","year":"2011","journal-title":"Cell"},{"key":"2026010816044905700_cit2","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1038\/nrc1098","volume":"3","author":"Fidler","year":"2003","journal-title":"Nat. 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Kulkarni (ORCID)","URL":"http:\/\/orcid.org\/0000-0003-3932-9092","name":"identifier","group":{"name":"author_identifiers","label":"IDENTIFIERS"}},{"value":"The Royal Society of Chemistry has an exclusive publication licence for this journal","name":"copyright_licence","group":{"name":"copyright_licensing","label":"COPYRIGHT AND LICENSING"}},{"value":"Single-blind","order":2,"name":"peer_review_method","group":{"name":"peer_review_method","label":"PEER REVIEW METHOD"}},{"value":"Received 2 May 2017; Accepted 16 August 2017; Advance Article published 6 September 2017; Version of Record published 24 October 2017","order":1,"name":"history","group":{"name":"publication_history","label":"PUBLICATION HISTORY"}}]}}