{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T13:41:50Z","timestamp":1792676510884,"version":"4.5.0"},"reference-count":40,"publisher":"Royal Society of Chemistry (RSC)","issue":"9","content-domain":{"domain":["rsc.org"],"crossmark-restriction":true},"short-container-title":["Org. Biomol. Chem."],"abstract":"<p>\n                    Chemical optimization of (\n                    <italic>S<\/italic>\n                    )-blebbistatin resulted in the discovery of (\n                    <italic>S<\/italic>\n                    )-3\u2032-hydroxyblebbistatin and (\n                    <italic>S<\/italic>\n                    )-3\u2032-aminoblebbistatin, myosin II inhibitors with superior research tool properties.\n                  <\/p>","DOI":"10.1039\/c7ob00006e","type":"journal-article","created":{"date-parts":[[2017,2,7]],"date-time":"2017-02-07T06:27:20Z","timestamp":1486448840000},"page":"2104-2118","update-policy":"https:\/\/doi.org\/10.1039\/rsc_crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Discovery of (S)-3\u2032-hydroxyblebbistatin and (S)-3\u2032-aminoblebbistatin: polar myosin II inhibitors with superior research tool properties"],"prefix":"10.1039","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2744-8951","authenticated-orcid":false,"given":"Sigrid","family":"Verhasselt","sequence":"first","affiliation":[{"name":"SynBioC Research Group"},{"name":"Department of Sustainable Organic Chemistry and Technology"},{"name":"Ghent University"},{"name":"9000 Ghent"},{"name":"Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9407-6721","authenticated-orcid":false,"given":"Bart I.","family":"Roman","sequence":"additional","affiliation":[{"name":"SynBioC Research Group"},{"name":"Department of Sustainable Organic Chemistry and Technology"},{"name":"Ghent University"},{"name":"9000 Ghent"},{"name":"Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"De Wever","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Cancer Research"},{"name":"Department of Radiation Oncology and Experimental Cancer Research"},{"name":"Ghent University"},{"name":"9000 Ghent"},{"name":"Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2455-8856","authenticated-orcid":false,"given":"Kristof","family":"Van Hecke","sequence":"additional","affiliation":[{"name":"Department of Inorganic and Physical Chemistry"},{"name":"Ghent University"},{"name":"9000 Ghent"},{"name":"Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7091-6864","authenticated-orcid":false,"given":"Rik","family":"Van Deun","sequence":"additional","affiliation":[{"name":"Department of Inorganic and Physical Chemistry"},{"name":"Ghent University"},{"name":"9000 Ghent"},{"name":"Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc E.","family":"Bracke","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Cancer Research"},{"name":"Department of Radiation Oncology and Experimental Cancer Research"},{"name":"Ghent University"},{"name":"9000 Ghent"},{"name":"Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4393-5327","authenticated-orcid":false,"given":"Christian V.","family":"Stevens","sequence":"additional","affiliation":[{"name":"SynBioC Research Group"},{"name":"Department of Sustainable Organic Chemistry and Technology"},{"name":"Ghent University"},{"name":"9000 Ghent"},{"name":"Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"292","published-online":{"date-parts":[[2017]]},"reference":[{"key":"C7OB00006E-(cit1)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1146\/annurev.biophys.050708.133751","volume":"39","author":"Sweeney","year":"2010","journal-title":"Annu. Rev. Biophys."},{"key":"C7OB00006E-(cit2)\/*[position()=1]","doi-asserted-by":"crossref","DOI":"10.1007\/978-1-4020-6519-4","volume-title":"Myosins: A superfamily of molecular motors","author":"Coluccio","year":"2008","unstructured":"L. M. Coluccio , Myosins: A superfamily of molecular motors, Springer, Netherlands, 2008"},{"key":"C7OB00006E-(cit3)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"778","DOI":"10.1038\/nrm2786","volume":"10","author":"Vicente-Manzanares","year":"2009","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"C7OB00006E-(cit4)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"41","DOI":"10.4155\/fmc.12.185","volume":"5","author":"Bond","year":"2012","journal-title":"Future Med. Chem."},{"key":"C7OB00006E-(cit5)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1007\/s10974-004-6060-7","volume":"25","author":"Limouze","year":"2004","journal-title":"J. Muscle Res. Cell Motil."},{"issue":"Supplement","key":"C7OB00006E-(cit6)\/*[position()=1]","first-page":"271A","volume":"12","author":"Cheung","year":"2001","journal-title":"Mol. Biol. Cell"},{"key":"C7OB00006E-(cit7)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"835","DOI":"10.1387\/ijdb.113336ld","volume":"55","author":"Derycke","year":"2011","journal-title":"Int. J. Dev. Biol."},{"key":"C7OB00006E-(cit8)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"992","DOI":"10.1016\/j.bbrc.2003.12.031","volume":"313","author":"Duxbury","year":"2004","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"C7OB00006E-(cit9)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"4725","DOI":"10.1158\/0008-5472.CAN-05-4236","volume":"66","author":"Betapudi","year":"2006","journal-title":"Cancer Res."},{"key":"C7OB00006E-(cit10)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1091\/mbc.E11-01-0039","volume":"23","author":"Ivkovic","year":"2012","journal-title":"Mol. Biol. Cell"},{"key":"C7OB00006E-(cit11)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1083\/jcb.200503059","volume":"170","author":"Lehmann","year":"2005","journal-title":"J. Cell Biol."},{"key":"C7OB00006E-(cit12)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.virol.2014.12.016","volume":"476","author":"Kumakura","year":"2015","journal-title":"Virology"},{"key":"C7OB00006E-(cit13)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"25120","DOI":"10.1038\/srep25120","volume":"6","author":"Gao","year":"2016","journal-title":"Sci. Rep."},{"key":"C7OB00006E-(cit14)\/*[position()=1]","first-page":"174","volume":"72","author":"Lum","year":"2014","journal-title":"Pathog. Dis."},{"key":"C7OB00006E-(cit15)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"4130","DOI":"10.1167\/iovs.05-0164","volume":"46","author":"Zhang","year":"2005","journal-title":"Invest. Ophthalmol. Visual Sci."},{"key":"C7OB00006E-(cit16)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1038\/labinvest.2009.142","volume":"90","author":"Si","year":"2010","journal-title":"Lab. Invest."},{"key":"C7OB00006E-(cit17)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1038\/mp.2015.103","volume":"21","author":"Young","year":"2016","journal-title":"Mol. Psychiatry"},{"key":"C7OB00006E-(cit18)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"1020","DOI":"10.1016\/j.bbrc.2004.06.045","volume":"320","author":"Kolega","year":"2004","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"C7OB00006E-(cit19)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"14832","DOI":"10.1021\/bi0490284","volume":"43","author":"Ramamurthy","year":"2004","journal-title":"Biochemistry"},{"key":"C7OB00006E-(cit20)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"1736","DOI":"10.1002\/ejoc.200500103","author":"Lucas-Lopez","year":"2005","journal-title":"Eur. J. Org. Chem."},{"key":"C7OB00006E-(cit21)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"584","DOI":"10.1021\/bi0483357","volume":"44","author":"Sakamoto","year":"2005","journal-title":"Biochemistry"},{"key":"C7OB00006E-(cit22)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"870","DOI":"10.1016\/j.bbagen.2012.04.003","volume":"1820","author":"Mikulich","year":"2012","journal-title":"Biochim. Biophys. Acta, Gen. Subj."},{"key":"C7OB00006E-(cit23)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"8211","DOI":"10.1002\/anie.201403540","volume":"53","author":"K\u00e9pir\u00f3","year":"2014","journal-title":"Angew. Chem., Int. Ed."},{"key":"C7OB00006E-(cit24)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"9402","DOI":"10.1073\/pnas.1202786109","volume":"109","author":"K\u00e9pir\u00f3","year":"2012","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"C7OB00006E-(cit25)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1007\/s00424-012-1147-2","volume":"464","author":"Swift","year":"2012","journal-title":"Pflugers Arch."},{"key":"C7OB00006E-(cit26)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1016\/j.hrthm.2006.12.047","volume":"4","author":"Fedorov","year":"2007","journal-title":"Heart Rhythm"},{"key":"C7OB00006E-(cit27)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"1141","DOI":"10.1083\/jcb.200706167","volume":"179","author":"Wong","year":"2007","journal-title":"J. Cell Biol."},{"key":"C7OB00006E-(cit28)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1038\/nature11317","volume":"489","author":"Shiba","year":"2012","journal-title":"Nature"},{"key":"C7OB00006E-(cit29)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"1743","DOI":"10.1126\/science.1081412","volume":"299","author":"Straight","year":"2003","journal-title":"Science"},{"key":"C7OB00006E-(cit30)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"26141","DOI":"10.1038\/srep26141","volume":"6","author":"V\u00e1rkuti","year":"2016","journal-title":"Sci. Rep."},{"key":"C7OB00006E-(cit31)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1002\/bscb.19780870310","volume":"87","author":"Pedersen","year":"1978","journal-title":"Bull. Soc. Chim. Belg."},{"key":"C7OB00006E-(cit32)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"C1148","DOI":"10.1152\/ajpcell.00551.2006","volume":"293","author":"Dou","year":"2007","journal-title":"Am. J. Physiol.: Cell Physiol."},{"key":"C7OB00006E-(cit33)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"1146","DOI":"10.1021\/jo026469p","volume":"68","author":"Vutukuri","year":"2003","journal-title":"J. Org. Chem."},{"key":"C7OB00006E-(cit34)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"4005","DOI":"10.1002\/anie.200800400","volume":"47","author":"Belardi","year":"2008","journal-title":"Angew. Chem., Int. Ed."},{"key":"C7OB00006E-(cit35)\/*[position()=1]","first-page":"2005\/113556A1","volume-title":"WO","author":"Fowler","year":"2005","unstructured":"K. W. Fowler , D.Huan, E. A.Kesicki, H. C.Ooi, A. R.Oliver, F.Rua and J.Treiberg, WO2005\/113556A1, 2005"},{"key":"C7OB00006E-(cit36)\/*[position()=1]","unstructured":"Center For Disease Control and Prevention. Diazomethane. http:\/\/www.cdc.gov\/niosh\/ipcsneng\/neng1256.html"},{"key":"C7OB00006E-(cit37)\/*[position()=1]","unstructured":"International Programme on Chemical Safety. Diazomethane. http:\/\/www.inchem.org\/documents\/icsc\/icsc\/eics1256.htm"},{"key":"C7OB00006E-(cit38)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"1465","DOI":"10.1093\/oxfordjournals.jbchem.a135616","volume":"99","author":"Kodama","year":"1986","journal-title":"J. Biochem."},{"key":"C7OB00006E-(cit39)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1038\/ncb734","volume":"4","author":"Cheung","year":"2002","journal-title":"Nat. Cell Biol."},{"key":"C7OB00006E-(cit41)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"1409","DOI":"10.1093\/jnci\/51.5.1409","volume":"51","author":"Soule","year":"1973","journal-title":"J. Natl. Cancer Inst."}],"container-title":["Organic &amp; Biomolecular Chemistry"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/pubs.rsc.org\/en\/content\/articlepdf\/2017\/OB\/C7OB00006E","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T20:37:20Z","timestamp":1782851840000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.rsc.org\/ob\/article\/15\/9\/2104-2118\/574336"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017]]},"references-count":40,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2017]]}},"URL":"https:\/\/doi.org\/10.1039\/c7ob00006e","relation":{},"ISSN":["1477-0520","1477-0539"],"issn-type":[{"value":"1477-0520","type":"print"},{"value":"1477-0539","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017]]},"assertion":[{"value":"This document is Similarity Check deposited","name":"similarity_check","explanation":{"URL":"https:\/\/www.crossref.org\/services\/similarity-check\/"},"group":{"name":"similarity_check","label":"SIMILARITY CHECK"}},{"value":"Supplementary Information","URL":"https:\/\/doi.org\/10.1039\/C7OB00006E","name":"related_data","group":{"name":"related_data","label":"RELATED DATA"}},{"value":"Crystal Structure Data","URL":"http:\/\/xlink.rsc.org?ccdc=true&msid=C7OB00006E","name":"related_data"},{"value":"Sigrid Verhasselt (ORCID)","URL":"http:\/\/orcid.org\/0000-0002-2744-8951","name":"identifier","group":{"name":"author_identifiers","label":"IDENTIFIERS"}},{"value":"Bart I. Roman (ORCID)","URL":"http:\/\/orcid.org\/0000-0002-9407-6721","name":"identifier","group":{"name":"author_identifiers","label":"IDENTIFIERS"}},{"value":"Bart I. Roman (ResearcherID)","URL":"http:\/\/www.researcherid.com\/rid\/C-8284-2012","name":"identifier","group":{"name":"author_identifiers","label":"IDENTIFIERS"}},{"value":"Kristof Van Hecke (ORCID)","URL":"http:\/\/orcid.org\/0000-0002-2455-8856","name":"identifier","group":{"name":"author_identifiers","label":"IDENTIFIERS"}},{"value":"Rik Van Deun (ORCID)","URL":"http:\/\/orcid.org\/0000-0001-7091-6864","name":"identifier","group":{"name":"author_identifiers","label":"IDENTIFIERS"}},{"value":"Christian V. Stevens (ORCID)","URL":"http:\/\/orcid.org\/0000-0003-4393-5327","name":"identifier","group":{"name":"author_identifiers","label":"IDENTIFIERS"}},{"value":"The Royal Society of Chemistry has an exclusive publication licence for this journal","name":"copyright_licence","group":{"name":"copyright_licensing","label":"COPYRIGHT AND LICENSING"}},{"value":"Single-blind","order":2,"name":"peer_review_method","group":{"name":"peer_review_method","label":"PEER REVIEW METHOD"}},{"value":"Received 2 January 2017; Accepted 7 February 2017; Accepted Manuscript published 7 February 2017; Advance Article published 21 February 2017; Version of Record published 1 March 2017","order":1,"name":"history","group":{"name":"publication_history","label":"PUBLICATION HISTORY"}}]}}