{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T03:57:55Z","timestamp":1782878275474,"version":"3.54.5"},"reference-count":28,"publisher":"Royal Society of Chemistry (RSC)","issue":"5","license":[{"start":{"date-parts":[[2019,12,20]],"date-time":"2019-12-20T00:00:00Z","timestamp":1576800000000},"content-version":"am","delay-in-days":353,"URL":"http:\/\/rsc.li\/journals-terms-of-use"}],"funder":[{"DOI":"10.13039\/501100001026","name":"National Breast Cancer Foundation","doi-asserted-by":"crossref","award":["CG-12-07"],"award-info":[{"award-number":["CG-12-07"]}],"id":[{"id":"10.13039\/501100001026","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000923","name":"Australian Research Council","doi-asserted-by":"crossref","award":["DP140104006"],"award-info":[{"award-number":["DP140104006"]}],"id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000923","name":"Australian Research Council","doi-asserted-by":"crossref","award":["DP160102836"],"award-info":[{"award-number":["DP160102836"]}],"id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001794","name":"University of Queensland","doi-asserted-by":"crossref","award":["UQFEL1831057"],"award-info":[{"award-number":["UQFEL1831057"]}],"id":[{"id":"10.13039\/501100001794","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["rsc.org"],"crossmark-restriction":true},"short-container-title":["Lab Chip"],"abstract":"<p>\n                    We report an integrated multi-molecular sensor (IMMS) platform for an entire sample-to-answer protocol encompassing melanoma cell capture in biological fluids, on-chip cell lysis, and combined quantification of intracellular\n                    <italic>BRAF<\/italic>\n                    <sup>V600E<\/sup>\n                    DNA and protein amounts.\n                  <\/p>","DOI":"10.1039\/c8lc00991k","type":"journal-article","created":{"date-parts":[[2018,12,19]],"date-time":"2018-12-19T22:04:17Z","timestamp":1545257057000},"page":"738-748","update-policy":"https:\/\/doi.org\/10.1039\/rsc_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["An integrated multi-molecular sensor for simultaneous\n                    <i>BRAF<\/i>\n                    <sup>V600E<\/sup>\n                    protein and DNA single point mutation detection in circulating tumour cells"],"prefix":"10.1039","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3180-6202","authenticated-orcid":false,"given":"Shuvashis","family":"Dey","sequence":"first","affiliation":[{"name":"Centre for Personalized Nanomedicine"},{"name":"Australian Institute for Bioengineering and Nanotechnology (AIBN)"},{"name":"The University of Queensland"},{"name":"Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2652-1985","authenticated-orcid":false,"given":"Kevin M.","family":"Koo","sequence":"additional","affiliation":[{"name":"Centre for Personalized Nanomedicine"},{"name":"Australian Institute for Bioengineering and Nanotechnology (AIBN)"},{"name":"The University of Queensland"},{"name":"Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaoran","family":"Wang","sequence":"additional","affiliation":[{"name":"Centre for Personalized Nanomedicine"},{"name":"Australian Institute for Bioengineering and Nanotechnology (AIBN)"},{"name":"The University of Queensland"},{"name":"Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8099-3863","authenticated-orcid":false,"given":"Abu A. I.","family":"Sina","sequence":"additional","affiliation":[{"name":"Centre for Personalized Nanomedicine"},{"name":"Australian Institute for Bioengineering and Nanotechnology (AIBN)"},{"name":"The University of Queensland"},{"name":"Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9569-0478","authenticated-orcid":false,"given":"Alain","family":"Wuethrich","sequence":"additional","affiliation":[{"name":"Centre for Personalized Nanomedicine"},{"name":"Australian Institute for Bioengineering and Nanotechnology (AIBN)"},{"name":"The University of Queensland"},{"name":"Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5516-1280","authenticated-orcid":false,"given":"Matt","family":"Trau","sequence":"additional","affiliation":[{"name":"Centre for Personalized Nanomedicine"},{"name":"Australian Institute for Bioengineering and Nanotechnology (AIBN)"},{"name":"The University of Queensland"},{"name":"Australia"},{"name":"School of Chemistry and Molecular Biosciences"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"292","published-online":{"date-parts":[[2019]]},"reference":[{"key":"C8LC00991K-(cit1)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1038\/nrc.2017.7","volume":"17","author":"Wan","year":"2017","journal-title":"Nat. Rev. Cancer"},{"key":"C8LC00991K-(cit2)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"1174","DOI":"10.1039\/C8LC00100F","volume":"18","author":"Gorgannezhad","year":"2018","journal-title":"Lab Chip"},{"key":"C8LC00991K-(cit3)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"224ra24","DOI":"10.1126\/scitranslmed.3007094","volume":"6","author":"Bettegowda","year":"2014","journal-title":"Sci. Transl. Med."},{"key":"C8LC00991K-(cit4)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"346ra92","DOI":"10.1126\/scitranslmed.aaf6219","volume":"8","author":"Tie","year":"2016","journal-title":"Sci. Transl. Med."},{"key":"C8LC00991K-(cit5)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"850","DOI":"10.1016\/j.molonc.2014.12.011","volume":"9","author":"Mohamed Suhaimi","year":"2015","journal-title":"Mol. Oncol."},{"key":"C8LC00991K-(cit6)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"19709","DOI":"10.1038\/srep19709","volume":"6","author":"Tsao","year":"2016","journal-title":"Sci. Rep."},{"key":"C8LC00991K-(cit7)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"949","DOI":"10.1038\/nature00766","volume":"417","author":"Davies","year":"2002","journal-title":"Nature"},{"key":"C8LC00991K-(cit8)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"855","DOI":"10.1016\/S0092-8674(04)00215-6","volume":"116","author":"Wan","year":"2004","journal-title":"Cell"},{"key":"C8LC00991K-(cit9)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"2507","DOI":"10.1056\/NEJMoa1103782","volume":"364","author":"Chapman","year":"2011","journal-title":"N. Engl. J. Med."},{"key":"C8LC00991K-(cit10)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1016\/S0140-6736(12)60868-X","volume":"380","author":"Hauschild","year":"2012","journal-title":"Lancet"},{"key":"C8LC00991K-(cit11)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1186\/1471-2407-14-13","volume":"14","author":"Ihle","year":"2014","journal-title":"BMC Cancer"},{"key":"C8LC00991K-(cit12)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"935","DOI":"10.1136\/jclinpath-2017-204367","volume":"70","author":"Brien","year":"2017","journal-title":"J. Clin. Pathol."},{"key":"C8LC00991K-(cit13)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"e0153576","DOI":"10.1371\/journal.pone.0153576","volume":"11","author":"Harle","year":"2016","journal-title":"PLoS One"},{"key":"C8LC00991K-(cit14)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1097\/PDM.0b013e31823b216f","volume":"21","author":"Halait","year":"2012","journal-title":"Diagn. Mol. Pathol."},{"key":"C8LC00991K-(cit15)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1016\/j.jmoldx.2012.09.001","volume":"15","author":"Colomba","year":"2013","journal-title":"J. Mol. Diagn."},{"key":"C8LC00991K-(cit16)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"3200","DOI":"10.1039\/C7LC00587C","volume":"17","author":"Koo","year":"2017","journal-title":"Lab Chip"},{"key":"C8LC00991K-(cit17)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1021\/acssensors.5b00157","volume":"1","author":"Dey","year":"2016","journal-title":"ACS Sens."},{"key":"C8LC00991K-(cit18)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1007\/s00216-006-0327-3","volume":"385","author":"Fox","year":"2006","journal-title":"Anal. Bioanal. Chem."},{"key":"C8LC00991K-(cit19)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"2585","DOI":"10.1039\/c3lc00014a","volume":"13","author":"Shahini","year":"2013","journal-title":"Lab Chip"},{"key":"C8LC00991K-(cit20)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1016\/j.bios.2016.11.024","volume":"89","author":"Koo","year":"2017","journal-title":"Biosens. Bioelectron."},{"key":"C8LC00991K-(cit21)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"1115","DOI":"10.1371\/journal.pbio.0040204","volume":"4","author":"Piepenburg","year":"2006","journal-title":"PLoS Biol."},{"key":"C8LC00991K-(cit22)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"10884","DOI":"10.1039\/C7NR09496E","volume":"10","author":"Wuethrich","year":"2018","journal-title":"Nanoscale"},{"key":"C8LC00991K-(cit23)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"061501","DOI":"10.1063\/1.4936300","volume":"9","author":"Vaidyanathan","year":"2015","journal-title":"Biomicrofluidics"},{"key":"C8LC00991K-(cit24)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"1704025","DOI":"10.1002\/smll.201704025","volume":"14","author":"Koo","year":"2018","journal-title":"Small"},{"key":"C8LC00991K-(cit25)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/j.microc.2017.10.012","volume":"137","author":"Wuethrich","year":"2018","journal-title":"Microchem. J."},{"key":"C8LC00991K-(cit26)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"1415","DOI":"10.7150\/thno.15250","volume":"6","author":"Koo","year":"2016","journal-title":"Theranostics"},{"key":"C8LC00991K-(cit27)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1021\/acssensors.6b00691","volume":"2","author":"Kelley","year":"2017","journal-title":"ACS Sens."},{"key":"C8LC00991K-(cit28)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1111\/pcmr.12097","volume":"26","author":"Behren","year":"2013","journal-title":"Pigm. Cell Melanoma Res."}],"container-title":["Lab on a Chip"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/pubs.rsc.org\/en\/content\/articlepdf\/2019\/LC\/C8LC00991K","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T03:11:27Z","timestamp":1782875487000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.rsc.org\/lc\/article\/19\/5\/738-748\/607029"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019]]},"references-count":28,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2019,2,26]]}},"URL":"https:\/\/doi.org\/10.1039\/c8lc00991k","relation":{},"ISSN":["1473-0197","1473-0189"],"issn-type":[{"value":"1473-0197","type":"print"},{"value":"1473-0189","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019]]},"assertion":[{"value":"This document is Similarity Check deposited","name":"similarity_check","explanation":{"URL":"https:\/\/www.crossref.org\/services\/similarity-check\/"},"group":{"name":"similarity_check","label":"SIMILARITY CHECK"}},{"value":"Supplementary Information","URL":"https:\/\/doi.org\/10.1039\/C8LC00991K","name":"related_data","group":{"name":"related_data","label":"RELATED DATA"}},{"value":"Shuvashis Dey (ORCID)","URL":"http:\/\/orcid.org\/0000-0003-3180-6202","name":"identifier","group":{"name":"author_identifiers","label":"IDENTIFIERS"}},{"value":"Kevin M. Koo (ORCID)","URL":"http:\/\/orcid.org\/0000-0002-2652-1985","name":"identifier","group":{"name":"author_identifiers","label":"IDENTIFIERS"}},{"value":"Abu A. I. Sina (ORCID)","URL":"http:\/\/orcid.org\/0000-0001-8099-3863","name":"identifier","group":{"name":"author_identifiers","label":"IDENTIFIERS"}},{"value":"Alain Wuethrich (ORCID)","URL":"http:\/\/orcid.org\/0000-0001-9569-0478","name":"identifier","group":{"name":"author_identifiers","label":"IDENTIFIERS"}},{"value":"Matt Trau (ORCID)","URL":"http:\/\/orcid.org\/0000-0001-5516-1280","name":"identifier","group":{"name":"author_identifiers","label":"IDENTIFIERS"}},{"value":"Single-blind","order":2,"name":"peer_review_method","group":{"name":"peer_review_method","label":"PEER REVIEW METHOD"}},{"value":"Received 19 September 2018; Accepted 19 December 2018;  Accepted Manuscript published 20 December 2018;  Advance Article published 9 January 2019;  Version of Record published 26 February 2019","order":1,"name":"history","group":{"name":"publication_history","label":"PUBLICATION HISTORY"}}]}}