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The blood-feeding habit is essential for these therapies, yet the molecular mechanisms underlying the process are poorly understood. In the present study, the transcriptome of Poecilobdella javanica from five time points (days 0, 1, 10, 20, and 30 separately) of blood feeding was sequenced with short paired-end reads. After stringent quality control, \u223c380 million high-quality reads were assembled using SOAPdenovo-Trans with optimal parameters into a non-redundant set of 48\u2009784 transcripts (\u2265100 base pairs), representing about 38 Mb of unique transcriptome sequence. The average length of the transcripts was 570 bp with N50 lengths of 5751 to 7413 bp among different time points. We have assessed the effect of sequence quality and various assembly parameters on the final assembly output. Functional categorization revealed the conservation of genes involved in various biological processes, such as basal transcription factors and ribosome biogenesis in eukaryotes. In addition, we found that DNA\/RNA related pathways were predominantly expressed in the starving state while fatty acid metabolism, the anticoagulant pathway, and amino acid biosynthesis were activated during blood feeding. The leech transcriptome provides a resource for gene discovery and development of functional molecular markers during clinical applications.<\/jats:p>\n                  <jats:p\/>","DOI":"10.1039\/c8mo00098k","type":"journal-article","created":{"date-parts":[[2018,9,13]],"date-time":"2018-09-13T22:04:23Z","timestamp":1536876263000},"page":"352-361","update-policy":"https:\/\/doi.org\/10.1039\/rsc_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["<i>De novo<\/i>\n                    assembly and comparative transcriptome characterization of\n                    <i>Poecilobdella javanica<\/i>\n                    provide insight into blood feeding of medicinal leeches"],"prefix":"10.1093","volume":"14","author":[{"given":"Zichao","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Life Science & Technology, Kunming University, Kunming Key Laboratory of Hydroecology Restoration of Dianchi Lake, Key Laboratory of Special Biological Resource Development & Utilization of Universities in Yunnan Province a , Kunming, 650214, China \u00a0 abclzc@aliyun.com \u00a0 wangdebin1202@sina.com"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanjie","family":"Wang","sequence":"additional","affiliation":[{"name":"Jules Stein Eye Institute, Department of Ophthalmology, University of California b , Los Angeles, CA 90095, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiangrong","family":"Tong","sequence":"additional","affiliation":[{"name":"Department of Life Science & Technology, Kunming University, Kunming Key Laboratory of Hydroecology Restoration of Dianchi Lake, Key Laboratory of Special Biological Resource Development & Utilization of Universities in Yunnan Province a , Kunming, 650214, China \u00a0 abclzc@aliyun.com \u00a0 wangdebin1202@sina.com"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuan","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Life Science & Technology, Kunming University, Kunming Key Laboratory of Hydroecology Restoration of Dianchi Lake, Key Laboratory of Special Biological Resource Development & Utilization of Universities in Yunnan Province a , Kunming, 650214, China \u00a0 abclzc@aliyun.com \u00a0 wangdebin1202@sina.com"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lijiang","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Life Science & Technology, Kunming University, Kunming Key Laboratory of Hydroecology Restoration of Dianchi Lake, Key Laboratory of Special Biological Resource Development & Utilization of Universities in Yunnan Province a , Kunming, 650214, China \u00a0 abclzc@aliyun.com \u00a0 wangdebin1202@sina.com"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Debin","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Life Science & Technology, Kunming University, Kunming Key Laboratory of Hydroecology Restoration of Dianchi Lake, Key Laboratory of Special Biological Resource Development & Utilization of Universities in Yunnan Province a , Kunming, 650214, China \u00a0 abclzc@aliyun.com \u00a0 wangdebin1202@sina.com"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4256-4512","authenticated-orcid":false,"given":"Yuqi","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Integrative Biology and Physiology, University of California, Los Angeles c , Los Angeles, CA 90095, USA \u00a0 zhaoyuqi616@gmail.com"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2018,9,14]]},"reference":[{"key":"2026010815041691300_cit1","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1186\/1471-2148-9-246","article-title":"Poly-paraphyly of Hirudinidae: many lineages of medicinal leeches","volume":"9","author":"Phillips","year":"2009","journal-title":"BMC Evol. 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