{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T02:08:54Z","timestamp":1790388534192,"version":"4.1.0"},"reference-count":52,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2019,4,16]],"date-time":"2019-04-16T00:00:00Z","timestamp":1555372800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"},{"start":{"date-parts":[[2020,4,16]],"date-time":"2020-04-16T00:00:00Z","timestamp":1586995200000},"content-version":"am","delay-in-days":366,"URL":"http:\/\/rsc.li\/journals-terms-of-use"}],"funder":[{"DOI":"10.13039\/501100007229","name":"Bijzonder Onderzoeksfonds","doi-asserted-by":"publisher","award":["IOP"],"award-info":[{"award-number":["IOP"]}],"id":[{"id":"10.13039\/501100007229","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003130","name":"Fonds Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["Unassigned"],"award-info":[{"award-number":["Unassigned"]}],"id":[{"id":"10.13039\/501100003130","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004385","name":"Universiteit Gent","doi-asserted-by":"publisher","award":["Unassigned"],"award-info":[{"award-number":["Unassigned"]}],"id":[{"id":"10.13039\/501100004385","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011899","name":"Vlaamse Vereniging voor Gastro-enterologie","doi-asserted-by":"publisher","award":["Unassigned"],"award-info":[{"award-number":["Unassigned"]}],"id":[{"id":"10.13039\/501100011899","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007229","name":"Bijzonder Onderzoeksfonds","doi-asserted-by":"crossref","award":["IOP"],"award-info":[{"award-number":["IOP"]}],"id":[{"id":"10.13039\/501100007229","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003130","name":"Fonds Wetenschappelijk Onderzoek","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003130","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004385","name":"Universiteit Gent","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004385","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100011899","name":"Vlaamse Vereniging voor Gastro-enterologie","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100011899","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["rsc.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2019,6,19]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Patients with chronic liver disease from different aetiologies show a light serum Cu isotopic composition compared to the reference population, with the enrichment in the 63Cu isotope correlating with the severity of the disease. However, the mechanisms underlying Cu isotope fractionation at the onset and during progression of the disease are still unclear. In this work, a common bile duct ligation (CBDL) murine model was used to investigate the effect of cholestasis-induced liver disease on the Cu isotopic composition. Wild type male and female mice underwent surgical ligation of the common bile duct and were sacrificed 2, 4 and 6 weeks, and 4, 6 and 8 weeks after the surgical intervention, respectively. The age- and gender-matched control mice underwent sham surgery. Disease progression was evaluated using serum bilirubin levels, hepatic pro-inflammatory chemokine levels and Metavir fibrosis score. CBDL-operated mice show an overall body enrichment in the light isotope 63Cu. The Cu isotopic composition of organs, bone and serum becomes gradually lighter compared to the sham-operated mice with increasing severity of the disease. The light Cu isotopic composition of the CBDL-operated mice might result from an altered Cu intake and\/or excretion. As the intestinal uptake of dietary Cu is largely mediated by transporters of Cu(i), mRNA and protein expression levels of two major metal transporters (CTR1 and DMT1) and Cu reductases (STEAP proteins and duodenal cytochrome B) were examined in the duodenal tissues as potential factors inducing Cu isotope fractionation. However, no significant differences in protein expression levels were observed between the CBDL- and sham-operated mice.<\/jats:p>","DOI":"10.1039\/c8mt00362a","type":"journal-article","created":{"date-parts":[[2019,4,16]],"date-time":"2019-04-16T09:34:43Z","timestamp":1555407283000},"page":"1093-1103","update-policy":"https:\/\/doi.org\/10.1039\/rsc_crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["Body distribution of stable copper isotopes during the progression of cholestatic liver disease induced by common bile duct ligation in mice"],"prefix":"10.1093","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8159-9961","authenticated-orcid":false,"given":"Marta","family":"Costas-Rodr\u00edguez","sequence":"first","affiliation":[{"name":"Department of Chemistry, Ghent University, Atomic & Mass Spectrometry \u2013 A&MS Research Unit, Campus Sterre, Krijgslaan 281-S12, BE-9000 Ghent, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9362-7475","authenticated-orcid":false,"given":"Sanne","family":"Van Campenhout","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine and Pediatrics, Ghent University, Corneel Heymanslaan 10-UZ-Entrance 12-Floor 01, BE-9000 Ghent, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7243-3161","authenticated-orcid":false,"given":"Agustina A M B","family":"Hastuti","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Ghent University, Atomic & Mass Spectrometry \u2013 A&MS Research Unit, Campus Sterre, Krijgslaan 281-S12, BE-9000 Ghent, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4862-9580","authenticated-orcid":false,"given":"Lindsey","family":"Devisscher","sequence":"additional","affiliation":[{"name":"Department of Basic and Applied Medical Sciences, Ghent University, Corneel Heymanslaan 10-UZ-Entrance 36-Floor 03, BE-9000 Ghent, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0647-9238","authenticated-orcid":false,"given":"Hans","family":"Van Vlierberghe","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine and Pediatrics, Ghent University, Corneel Heymanslaan 10-UZ-Entrance 12-Floor 01, BE-9000 Ghent, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1884-3853","authenticated-orcid":false,"given":"Frank","family":"Vanhaecke","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Ghent University, Atomic & Mass Spectrometry \u2013 A&MS Research Unit, Campus Sterre, Krijgslaan 281-S12, BE-9000 Ghent, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2019,4,16]]},"reference":[{"key":"2021121516075062800_cit1","doi-asserted-by":"crossref","first-page":"846S","DOI":"10.1093\/ajcn\/88.3.846S","volume":"88","author":"L\u00f6nnerdal","year":"2008","journal-title":"Am. J. Clin. Nutr."},{"key":"2021121516075062800_cit2","doi-asserted-by":"crossref","first-page":"826S","DOI":"10.1093\/ajcn\/88.3.826S","volume":"88","author":"Prohaska","year":"2008","journal-title":"Am. J. Clin. Nutr."},{"key":"2021121516075062800_cit3","doi-asserted-by":"crossref","first-page":"6836","DOI":"10.1073\/pnas.111057298","volume":"98","author":"Kuo","year":"2001","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"2021121516075062800_cit4","doi-asserted-by":"crossref","first-page":"6842","DOI":"10.1073\/pnas.111058698","volume":"98","author":"Lee","year":"2001","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"2021121516075062800_cit5","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1016\/j.mam.2012.07.011","volume":"34","author":"Kim","year":"2013","journal-title":"Mol. Aspects Med."},{"key":"2021121516075062800_cit6","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1016\/j.cmet.2006.08.009","volume":"4","author":"Nose","year":"2006","journal-title":"Cell Metab."},{"key":"2021121516075062800_cit7","doi-asserted-by":"crossref","first-page":"E4279","DOI":"10.1073\/pnas.1311749110","volume":"110","author":"\u00d6hrvik","year":"2013","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"2021121516075062800_cit8","doi-asserted-by":"crossref","first-page":"C1525","DOI":"10.1152\/ajpcell.00480.2002","volume":"284","author":"Arredondo","year":"2003","journal-title":"Am. J. Physiol.: Cell Physiol."},{"key":"2021121516075062800_cit9","doi-asserted-by":"crossref","first-page":"79","DOI":"10.4067\/S0716-97602006000100009","volume":"39","author":"Garrick","year":"2006","journal-title":"Biol. Res."},{"key":"2021121516075062800_cit10","doi-asserted-by":"crossref","first-page":"830S","DOI":"10.1093\/ajcn\/88.3.830S","volume":"88","author":"Gonz\u00e1lez","year":"2008","journal-title":"Am. J. Clin. Nutr."},{"key":"2021121516075062800_cit11","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1016\/j.bbrc.2005.03.023","volume":"330","author":"Kn\u00f6pfel","year":"2005","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"2021121516075062800_cit12","doi-asserted-by":"crossref","first-page":"1011","DOI":"10.1152\/physrev.00004.2006","volume":"87","author":"Lutsenko","year":"2007","journal-title":"Physiol. Rev."},{"key":"2021121516075062800_cit13","doi-asserted-by":"crossref","first-page":"1039","DOI":"10.1016\/0006-2952(90)90282-P","volume":"39","author":"Houwen","year":"1990","journal-title":"Biochem. Pharmacol."},{"key":"2021121516075062800_cit14","doi-asserted-by":"crossref","DOI":"10.1007\/978-1-4757-9432-8","volume-title":"Biochemistry of Copper","author":"Linder","year":"1991"},{"key":"2021121516075062800_cit15","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1039\/C4MT00319E","volume":"7","author":"Costas-Rodr\u00edguez","year":"2015","journal-title":"Metallomics"},{"key":"2021121516075062800_cit16","doi-asserted-by":"crossref","first-page":"30683","DOI":"10.1038\/srep30683","volume":"6","author":"Lauwens","year":"2016","journal-title":"Sci. Rep."},{"key":"2021121516075062800_cit17","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1016\/j.talanta.2018.07.011","volume":"189","author":"Lauwens","year":"2018","journal-title":"Talanta"},{"key":"2021121516075062800_cit18","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1039\/c3ja30349g","volume":"28","author":"Aramend\u00eda","year":"2013","journal-title":"J. Anal. At. Spectrom."},{"key":"2021121516075062800_cit19","doi-asserted-by":"crossref","first-page":"982","DOI":"10.1073\/pnas.1415151112","volume":"112","author":"Balter","year":"2015","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"2021121516075062800_cit20","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1039\/C4MT00269E","volume":"7","author":"T\u00e9louk","year":"2015","journal-title":"Metallomics"},{"key":"2021121516075062800_cit21","doi-asserted-by":"crossref","first-page":"1177","DOI":"10.1039\/C6MT00102E","volume":"8","author":"Bondanese","year":"2016","journal-title":"Metallomics"},{"key":"2021121516075062800_cit22","doi-asserted-by":"crossref","first-page":"44533","DOI":"10.1038\/srep44533","volume":"7","author":"Cadiou","year":"2017","journal-title":"Sci. Rep."},{"key":"2021121516075062800_cit23","doi-asserted-by":"crossref","first-page":"2385","DOI":"10.1007\/s00216-018-0909-x","volume":"410","author":"Fl\u00f3rez","year":"2018","journal-title":"Anal. Bioanal. Chem."},{"key":"2021121516075062800_cit24","doi-asserted-by":"crossref","first-page":"1921","DOI":"10.1023\/A:1005615306596","volume":"45","author":"Ljubuncic","year":"2000","journal-title":"Dig. Dis. Sci."},{"key":"2021121516075062800_cit25","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/S0925-4439(02)00225-9","volume":"1637","author":"Aboutwerat","year":"2003","journal-title":"Biochim. Biophys. Acta"},{"key":"2021121516075062800_cit26","doi-asserted-by":"crossref","first-page":"2233","DOI":"10.2741\/e539","volume":"4","author":"Arduini","year":"2012","journal-title":"Front. Biosci."},{"key":"2021121516075062800_cit27","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1038\/s41598-017-18958-8","volume":"8","author":"Koh","year":"2018","journal-title":"Sci. Rep."},{"key":"2021121516075062800_cit28","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1016\/j.jhep.2009.05.012","volume":"51","author":"Wagner","year":"2009","journal-title":"J. Hepatol."},{"key":"2021121516075062800_cit29","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1055\/s-0030-1253227","volume":"30","author":"Kosters","year":"2010","journal-title":"Semin. Liver Dis."},{"key":"2021121516075062800_cit30","doi-asserted-by":"crossref","first-page":"951","DOI":"10.1002\/hep.28373","volume":"63","author":"Vartak","year":"2016","journal-title":"Hepatology"},{"key":"2021121516075062800_cit31","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1186\/s12918-015-0229-0","volume":"9","author":"Abshagen","year":"2015","journal-title":"BMC Syst. Biol."},{"key":"2021121516075062800_cit32","doi-asserted-by":"crossref","first-page":"598","DOI":"10.1016\/j.jhep.2007.07.006","volume":"47","author":"Goodman","year":"2007","journal-title":"J. Hepatol."},{"key":"2021121516075062800_cit33","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1039\/C6JA00433D","volume":"32","author":"Lauwens","year":"2017","journal-title":"J. Anal. At. Spectrom."},{"key":"2021121516075062800_cit34","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"2021121516075062800_cit35","doi-asserted-by":"crossref","first-page":"1470","DOI":"10.1039\/c3mt00151b","volume":"5","author":"Balter","year":"2013","journal-title":"Metallomics"},{"key":"2021121516075062800_cit36","doi-asserted-by":"crossref","first-page":"2015","DOI":"10.1039\/C6JA00195E","volume":"31","author":"Miller","year":"2016","journal-title":"J. Anal. At. Spectrom."},{"key":"2021121516075062800_cit37","doi-asserted-by":"crossref","first-page":"960","DOI":"10.1016\/j.biocel.2013.01.018","volume":"45","author":"Wee","year":"2013","journal-title":"Int. J. Biochem. Cell Biol."},{"key":"2021121516075062800_cit38","doi-asserted-by":"crossref","first-page":"1388","DOI":"10.1182\/blood-2006-02-003681","volume":"108","author":"Ohgami","year":"2006","journal-title":"Blood"},{"key":"2021121516075062800_cit39","doi-asserted-by":"crossref","first-page":"1901","DOI":"10.1016\/j.febslet.2008.05.010","volume":"582","author":"Wyman","year":"2008","journal-title":"FEBS Lett."},{"key":"2021121516075062800_cit40","doi-asserted-by":"crossref","first-page":"883","DOI":"10.3748\/wjg.v8.i5.883","volume":"8","author":"Xu","year":"2002","journal-title":"World J. Gastroenterol."},{"key":"2021121516075062800_cit41","doi-asserted-by":"crossref","first-page":"14851","DOI":"10.1074\/jbc.M111.314922","volume":"287","author":"Zhang","year":"2012","journal-title":"J. Biol. Chem."},{"key":"2021121516075062800_cit42","doi-asserted-by":"crossref","first-page":"1203","DOI":"10.1152\/ajplegacy.1964.207.6.1203","volume":"207","author":"Owen","year":"1964","journal-title":"Am. J. Physiol."},{"key":"2021121516075062800_cit43","doi-asserted-by":"crossref","first-page":"e38327","DOI":"10.1371\/journal.pone.0038327","volume":"7","author":"Gray","year":"2012","journal-title":"PLoS One"},{"key":"2021121516075062800_cit44","doi-asserted-by":"crossref","first-page":"1805","DOI":"10.1039\/C7JA00134G","volume":"32","author":"Costas-Rodr\u00edguez","year":"2017","journal-title":"J. Anal. At. Spectrom."},{"key":"2021121516075062800_cit45","doi-asserted-by":"crossref","first-page":"960S","DOI":"10.1093\/ajcn\/67.5.960S","volume":"67","author":"Turnlund","year":"1998","journal-title":"Am. J. Clin. Nutr."},{"key":"2021121516075062800_cit46","volume-title":"Advanced nutrition and human metabolism","author":"Gropper","year":"2013"},{"key":"2021121516075062800_cit47","doi-asserted-by":"crossref","first-page":"e00073","DOI":"10.1002\/phy2.73","volume":"1","author":"Alaish","year":"2013","journal-title":"Physiol. Rep."},{"key":"2021121516075062800_cit48","doi-asserted-by":"crossref","first-page":"5955","DOI":"10.1073\/pnas.1814047116","volume":"13","author":"Miller","year":"2019","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"2021121516075062800_cit49","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/S0168-8278(96)80194-2","volume":"24","author":"Dijkstra","year":"1996","journal-title":"J. Hepatol."},{"key":"2021121516075062800_cit50","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1002\/(SICI)1099-0461(2000)14:4<210::AID-JBT5>3.0.CO;2-G","volume":"14","author":"Schilsky","year":"2000","journal-title":"J. Biochem. Mol. Toxicol."},{"key":"2021121516075062800_cit51","doi-asserted-by":"crossref","first-page":"1809","DOI":"10.1039\/C7MT00248C","volume":"9","author":"Tennant","year":"2017","journal-title":"Metallomics"},{"key":"2021121516075062800_cit52","author":"Poyo"}],"container-title":["Metallomics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/metallomics\/article-pdf\/11\/6\/1093\/41775798\/c8mt00362a.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/metallomics\/article-pdf\/11\/6\/1093\/41775798\/c8mt00362a.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,12,15]],"date-time":"2021-12-15T11:32:37Z","timestamp":1639567957000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/metallomics\/article\/11\/6\/1093\/5952398"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,4,16]]},"references-count":52,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2019,6,19]]}},"URL":"https:\/\/doi.org\/10.1039\/c8mt00362a","relation":{},"ISSN":["1756-5901","1756-591X"],"issn-type":[{"value":"1756-5901","type":"print"},{"value":"1756-591X","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2019,6]]},"published":{"date-parts":[[2019,4,16]]},"assertion":[{"value":"This document is Similarity Check deposited","name":"similarity_check","explanation":{"URL":"https:\/\/www.crossref.org\/services\/similarity-check\/"},"group":{"name":"similarity_check","label":"SIMILARITY CHECK"}},{"value":"Supplementary Information","URL":"https:\/\/doi.org\/10.1039\/C8MT00362A","name":"related_data","group":{"name":"related_data","label":"RELATED DATA"}},{"value":"Marta Costas-Rodr\u00edguez (ORCID)","URL":"http:\/\/orcid.org\/0000-0002-8159-9961","name":"identifier","group":{"name":"author_identifiers","label":"IDENTIFIERS"}},{"value":"Marta Costas-Rodr\u00edguez (ResearcherID)","URL":"http:\/\/www.researcherid.com\/rid\/K-3862-2012","name":"identifier","group":{"name":"author_identifiers","label":"IDENTIFIERS"}},{"value":"Sanne Van Campenhout (ORCID)","URL":"http:\/\/orcid.org\/0000-0001-9362-7475","name":"identifier","group":{"name":"author_identifiers","label":"IDENTIFIERS"}},{"value":"Agustina A. M. B. Hastuti (ORCID)","URL":"http:\/\/orcid.org\/0000-0001-7243-3161","name":"identifier","group":{"name":"author_identifiers","label":"IDENTIFIERS"}},{"value":"Lindsey Devisscher (ORCID)","URL":"http:\/\/orcid.org\/0000-0003-4862-9580","name":"identifier","group":{"name":"author_identifiers","label":"IDENTIFIERS"}},{"value":"Hans Van Vlierberghe (ORCID)","URL":"http:\/\/orcid.org\/0000-0003-0647-9238","name":"identifier","group":{"name":"author_identifiers","label":"IDENTIFIERS"}},{"value":"Frank Vanhaecke (ORCID)","URL":"http:\/\/orcid.org\/0000-0002-1884-3853","name":"identifier","group":{"name":"author_identifiers","label":"IDENTIFIERS"}},{"value":"Single-blind","order":2,"name":"peer_review_method","group":{"name":"peer_review_method","label":"PEER REVIEW METHOD"}},{"value":"Received 17 December 2018; Accepted 16 April 2019;  Accepted Manuscript published 16 April 2019;  Advance Article published 25 April 2019;  Version of Record published 19 June 2019","order":1,"name":"history","group":{"name":"publication_history","label":"PUBLICATION HISTORY"}}]}}