{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T12:25:42Z","timestamp":1790252742701,"version":"4.1.0"},"reference-count":34,"publisher":"Springer Science and Business Media LLC","issue":"4","license":[{"start":{"date-parts":[[2018,4,1]],"date-time":"2018-04-01T00:00:00Z","timestamp":1522540800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2018,4,1]],"date-time":"2018-04-01T00:00:00Z","timestamp":1522540800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["31100704"],"award-info":[{"award-number":["31100704"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"crossref","award":["2017M612462"],"award-info":[{"award-number":["2017M612462"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003819","name":"Natural Science Foundation of Hubei Province","doi-asserted-by":"crossref","award":["2015CFC892"],"award-info":[{"award-number":["2015CFC892"]}],"id":[{"id":"10.13039\/501100003819","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003819","name":"Natural Science Foundation of Hubei Province","doi-asserted-by":"crossref","award":["2015CFB696"],"award-info":[{"award-number":["2015CFB696"]}],"id":[{"id":"10.13039\/501100003819","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003819","name":"Natural Science Foundation of Hubei Province","doi-asserted-by":"crossref","award":["2017CFB732"],"award-info":[{"award-number":["2017CFB732"]}],"id":[{"id":"10.13039\/501100003819","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Photochem Photobiol Sci"],"published-print":{"date-parts":[[2018,4]]},"DOI":"10.1039\/c8pp00071a","type":"journal-article","created":{"date-parts":[[2018,3,7]],"date-time":"2018-03-07T06:33:48Z","timestamp":1520404428000},"page":"474-481","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Facile synthesis of a two-photon fluorescent probe based on pyrimidine 2-isothiocyanate and its application in bioimaging"],"prefix":"10.1007","volume":"17","author":[{"given":"Jie","family":"Yang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huirong","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanna","family":"Hou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Tu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5450-5528","authenticated-orcid":false,"given":"Bo","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,10,27]]},"reference":[{"key":"17040474_CR1","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1002\/jemt.10418","volume":"63","author":"B R Master","year":"2004","unstructured":"B. R. Master and P. T. C. So, Antecedents of two-photon excitation laser scanning microscopy, Microsc. Res. Tech., 2004, 63, 3\u201311.","journal-title":"Microsc. Res. Tech."},{"key":"17040474_CR2","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1126\/science.2321027","volume":"248","author":"W Denk","year":"1990","unstructured":"W. Denk, J. H. Strickler and W. W. Webb, Two-photon laser scanning fluorescence microscopy, Science, 1990, 248, 73\u201376.","journal-title":"Science"},{"key":"17040474_CR3","doi-asserted-by":"publisher","first-page":"1869","DOI":"10.1126\/science.1070051","volume":"296","author":"M J Miller","year":"2002","unstructured":"M. J. Miller, S. H. Wei, I. Parker and M. D. Cahalan, Two-photon imaging of lymphocyte motility and antigen response in intact lymph node, Science, 2002, 296, 1869\u20131873.","journal-title":"Science"},{"key":"17040474_CR4","doi-asserted-by":"publisher","first-page":"805","DOI":"10.1038\/nmeth.1243","volume":"5","author":"M Mank","year":"2008","unstructured":"M. Mank, A. F. Santos, S. Direnberger, T. D. Mrsic-Flogel, S. B. Hofer, V. Stein, T. Hendel, D. F. Reiff, C. Levelt, A. Borst, T. Bonhoeffer, M. H\u00fcbener and O. Griesbeck, A genetically encoded calcium indicator for chronic in vivo two-photon imaging, Nat. Methods, 2008, 5, 805\u2013811.","journal-title":"Nat. Methods"},{"key":"17040474_CR5","doi-asserted-by":"publisher","first-page":"11377","DOI":"10.1073\/pnas.1514209112","volume":"112","author":"C Tischbirek","year":"2015","unstructured":"C. Tischbirek, A. Birkner, H. Jia, B. Sakmann and A. Konnerth, Deep two-photon brain imaging with a red-shifted fluorometric Ca2+ indicator, Proc. Natl. Acad. Sci. U. S. A., 2015, 112, 11377\u201311382.","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"17040474_CR6","doi-asserted-by":"publisher","first-page":"1302","DOI":"10.1039\/C4CS00173G","volume":"44","author":"Y Park","year":"2015","unstructured":"Y. Park, K. T. Lee, Y. D. Suh and T. Hyeon, Upconverting nanoparticles: a versatile platform for wide-field two-photon microscopy and multi-modal in vivo imaging, Chem. Soc. Rev., 2015, 44, 1302\u20131317.","journal-title":"Chem. Soc. Rev."},{"key":"17040474_CR7","doi-asserted-by":"publisher","first-page":"31","DOI":"10.3389\/fnana.2016.00031","volume":"10","author":"S P Amato","year":"2016","unstructured":"S. P. Amato, F. Pan, J. Schwartz and T. M. Ragan, Whole brain imaging with serial two-photon tomography, Front. Neuroanat., 2016, 10, 31.","journal-title":"Front. Neuroanat."},{"key":"17040474_CR8","doi-asserted-by":"publisher","first-page":"2857","DOI":"10.1002\/adfm.201500010","volume":"25","author":"Y Gao","year":"2015","unstructured":"Y. Gao, G. Feng, T. Jiang, C. Goh, L. Ng, B. Liu, L. Yang, J. Hua and H. Tian, Biocompatible nanoparticles based on diketo-pyrrolo-pyrrole (DPP) with aggregation-induced red\/ NIR emission for in vivo two-photon fluorescence imaging, Adv. Funct. Mater., 2015, 25, 2857\u20132866.","journal-title":"Adv. Funct. Mater."},{"key":"17040474_CR9","doi-asserted-by":"publisher","first-page":"169","DOI":"10.1002\/anie.201407537","volume":"54","author":"J Schmitt","year":"2015","unstructured":"J. Schmitt, V. Heitz, A. Sour, F. Bolze, H. Ftouni, J. Nicoud, L. Flamigni and B. Ventura, Diketopyrrolopyrrole-porphyrin conjugates with high two-photon absorption and singlet oxygen generation for two-photon photodynamic therapy, Angew. Chem., Int. Ed., 2015, 54, 169\u2013173.","journal-title":"Angew. Chem., Int. Ed."},{"key":"17040474_CR10","first-page":"12819","volume":"6","author":"D Cvejn","year":"2016","unstructured":"D. Cvejn, E. Michail, K. Seintis, M. Klikar, O. Pytela, T. Mikysek, N. Almonasy, M. Ludwig, V. Giannetas, M. Fakis and F. Bures, Solvent and branching effect on the two-photon absorption properties of push-pull triphenylamine derivatives, RSCAdv., 2016, 6, 12819\u201312828.","journal-title":"RSCAdv."},{"key":"17040474_CR11","doi-asserted-by":"publisher","first-page":"5199","DOI":"10.1039\/C7TC01266G","volume":"5","author":"D Chen","year":"2017","unstructured":"D. Chen, C. Zhong, Y. Zhao, L. Nan, Y. Liu and J. Qin, A two-dimensional molecule with a large conjugation degree: synthesis, two-photon absorption and charge transport ability, J. Mater. Chem. C, 2017, 5, 5199\u20135206.","journal-title":"J. Mater. Chem. C"},{"key":"17040474_CR12","doi-asserted-by":"publisher","first-page":"174","DOI":"10.1016\/j.dyepig.2016.10.015","volume":"137","author":"R Liu","year":"2017","unstructured":"R. Liu, M. Shu, J. Hu, S. Zhu, H. Shi and H. Zhu, Starshaped D-n-A compounds with a 1,3,5-triazine core and N-aryl chromophore substituted fluorene arms: synthesis, aggregation induced emission and two-photon absorption, Dyes Pigm., 2017, 137, 174\u2013181.","journal-title":"Dyes Pigm."},{"key":"17040474_CR13","doi-asserted-by":"publisher","first-page":"1736","DOI":"10.1002\/asia.201700159","volume":"12","author":"L G Lukasiewicz","year":"2017","unstructured":"L. G. Lukasiewicz, H. G. Ryu, A. Mikhaylov, C. Azarias, M. Banasiewicz, B. Kozankiewicz, K. H. Ahn, D. Jacquemin, A. Rebane and D. T. Gryko, Symmetry breaking in pyrrolo [3, 2-b] pyrroles: synthesis, solvatofluorochromism and two-photon absorption, Chem. - Asian J., 2017, 12, 1736\u20131748.","journal-title":"Chem. - Asian J."},{"key":"17040474_CR14","doi-asserted-by":"publisher","first-page":"3295","DOI":"10.1021\/acs.chemmater.7b00676","volume":"29","author":"S Wang","year":"2017","unstructured":"S. Wang, Z. Li, X. Liu, S. Phan, F. Lv, K. D. Belfield, S. Wang and K. S. Schanze, Two-photon absorption of cationic conjugated polyelectrolytes: effects of aggregation and application to 2-photon-sensitized fluorescence from green fluorescent protein, Chem. Mater., 2017, 29, 3295\u20133303.","journal-title":"Chem. Mater."},{"key":"17040474_CR15","doi-asserted-by":"publisher","first-page":"4685","DOI":"10.1039\/b707909e","volume":"17","author":"Z Liu","year":"2007","unstructured":"Z. Liu, T. Chen, B. Liu, Z. Huang, T. Huang, S. Li, Y. Xu and J. Qin, Two-photon absorption of a series of V-shape molecules: the influence of acceptor\u2019s strength on two-photon absorption in a noncentrosymmetric D-n-A-n-D system, J. Mater. Chem., 2007, 17, 4685\u20134689.","journal-title":"J. Mater. Chem."},{"key":"17040474_CR16","doi-asserted-by":"publisher","first-page":"1245","DOI":"10.1021\/cr050054x","volume":"108","author":"G S He","year":"2008","unstructured":"G. S. He, L. Tan, Q. Zheng and P. N. Prasad, Multiphoton absorbing materials: molecular designs, characterizations, and applications, Chem. Rev., 2008, 108, 1245\u20131330.","journal-title":"Chem. Rev."},{"key":"17040474_CR17","first-page":"2260","volume-title":"Chem. Commun.","author":"Z Liu","year":"2008","unstructured":"Z. Liu, P. Shao, Z. Huang, B. Liu, T. Chen and J. Qin, Two-photon absorption enhancement induced by aggregation due to intermolecular hydrogen bonding in V-shaped 2-hydroxypyrimidine derivatives, Chem. Commun., 2008, 2260\u20132262."},{"key":"17040474_CR18","doi-asserted-by":"publisher","first-page":"2921","DOI":"10.1039\/b702004j","volume":"17","author":"B Liu","year":"2007","unstructured":"B. Liu, H. Zhang, J. Liu, Y. Zhao, Q. Luo and Z. Huang, Novel pyrimidine-based amphiphilic molecules: synthesis, spectroscopic properties and applications in two-photon fluorescence microscopic imaging, J. Mater. Chem., 2007, 17, 2921\u20132929.","journal-title":"J. Mater. Chem."},{"key":"17040474_CR19","doi-asserted-by":"publisher","first-page":"668","DOI":"10.1002\/asia.200800402","volume":"4","author":"L Li","year":"2009","unstructured":"L. Li, Y. Tian, J. Yang, P. Sun, J. Wu, H. Zhou, S. Zhang, B. Jin, X. Xing, C. Wang, M. Li, G. Cheng, H. Tang, W. Huang, X. Tao and M. Jiang, Facile synthesis and sys tematic investigations of a series of novel bent-shaped two-photon absorption chromophores based on pyrimidine, Chem. - Asian J., 2009, 4, 668\u2013680.","journal-title":"Chem. - Asian J."},{"key":"17040474_CR20","doi-asserted-by":"publisher","first-page":"7869","DOI":"10.1039\/C4TC00841C","volume":"2","author":"J Malval","year":"2014","unstructured":"J. Malval, S. Achelle, L. Bodiou, A. Spangenberg, L. C. Gomez, O. Soppera and F. R. Guen, Two-photon absorption in a conformationally twisted D-n-A oligomer: a synergic photosensitizing approach for multiphoton lithography, J. Mater. Chem. C, 2014, 2, 7869\u20137880.","journal-title":"J. Mater. Chem. C"},{"key":"17040474_CR21","doi-asserted-by":"publisher","first-page":"3036","DOI":"10.1021\/cr500271a","volume":"115","author":"M E Cinar","year":"2015","unstructured":"M. E. Cinar and T. Ozturk, Thienothiophenes, dithie-nothiophenes, and thienoacenes: syntheses, oligomers, polymers, and properties, Chem. Rev., 2015, 115, 3036\u20133140.","journal-title":"Chem. Rev."},{"key":"17040474_CR22","first-page":"20068","volume":"7","author":"Q Zhang","year":"2017","unstructured":"Q. Zhang, R. Guan, X. Tian, L. Luo, H. Zhou, S. Li, J. Wu and Y. Tian, Small water-soluble pyrimidine hexafluorophosphate derivatives with high two-photon absorption activities in the near-IR region and their biological applications, RSCAdv., 2017, 7, 20068\u201320075.","journal-title":"RSCAdv."},{"key":"17040474_CR23","doi-asserted-by":"publisher","first-page":"163","DOI":"10.2174\/157017912799829067","volume":"9","author":"S Achelle","year":"2012","unstructured":"S. Achelle and N. Pl\u00e9, Pyrimidine ring as building block for the synthesis of functionalized n-conjugated materials, Curr. Org. Synth., 2012, 9, 163\u2013187.","journal-title":"Curr. Org. Synth."},{"key":"17040474_CR24","doi-asserted-by":"publisher","first-page":"4343","DOI":"10.1039\/c2jm14766a","volume":"22","author":"D Chen","year":"2012","unstructured":"D. Chen, C. Zhong, X. Dong, Z. Liu and J. Qin, A new building block, bis (thiophene vinyl)-pyrimidine, for constructing excellent two-photon absorption materials: synthesis, crystal structure and properties, J. Mater. Chem., 2012, 22, 4343\u20134348.","journal-title":"J. Mater. Chem."},{"key":"17040474_CR25","doi-asserted-by":"publisher","first-page":"263","DOI":"10.1016\/j.dyepig.2013.11.013","volume":"102","author":"H Wang","year":"2014","unstructured":"H. Wang, Q. Zhang, J. Zhang, L. Li, Q. Zhang, S. Li, S. Zhang, J. Wu and Y. Tian, Synthesis, two-photon absorption properties and bioimaging applications of mono-, di-and hexa-branched pyrimidine derivatives, Dyes Pigm., 2014, 102, 263\u2013272.","journal-title":"Dyes Pigm."},{"key":"17040474_CR26","doi-asserted-by":"publisher","first-page":"5958","DOI":"10.1016\/j.tetlet.2007.06.122","volume":"48","author":"B Liu","year":"2007","unstructured":"B. Liu, X. L. Hu, J. Liu, Y. D. Zhao and Z. L. Huang, Synthesis and photophysical properties of novel pyrimidine-based two-photon absorption chromophores, Tetrahedron Lett., 2007, 48, 5958\u20135962.","journal-title":"Tetrahedron Lett."},{"key":"17040474_CR27","doi-asserted-by":"publisher","first-page":"6355","DOI":"10.1021\/ja952591c","volume":"118","author":"K Ohkata","year":"1996","unstructured":"K. Ohkata, M. Ohsugi, K. Yamamoto, M. Ohsawa and K. Akiba, Structural features of tetraazathiapentalenes fused with pyrimidine and\/or pyridine rings. experimental evaluation of the nature of hypervalent N-S-N bond by restricted internal rotation of the pyrimidine ring, J. Am. Chem. Soc., 1996, 118, 6355\u20136369.","journal-title":"J. Am. Chem. Soc."},{"key":"17040474_CR28","doi-asserted-by":"publisher","first-page":"294","DOI":"10.1021\/j100248a024","volume":"89","author":"G Jones","year":"1985","unstructured":"G. Jones, W. R. Jackson, C. Y. Choi and W. R. Bergmark, Solvent effects on emission yield and lifetime for coumarin laser dyes. Requirements for a rotatory decay mechanism, J. Phys. Chem., 1985, 89, 294\u2013300.","journal-title":"J. Phys. Chem."},{"key":"17040474_CR29","doi-asserted-by":"publisher","first-page":"39218","DOI":"10.1039\/C5RA05736A","volume":"5","author":"S Achelle","year":"2015","unstructured":"S. Achelle, A. Barsella, B. Caro and F. R. Guen, Donorlinker-acceptor (D-n-A) diazine chromophores with extended n-conjugated cores: synthesis, photophysical and second order nonlinear optical properties, RSC Adv., 2015, 5, 39218\u201339227.","journal-title":"RSC Adv."},{"key":"17040474_CR30","doi-asserted-by":"publisher","first-page":"991","DOI":"10.1021\/j100678a001","volume":"75","author":"J N Demas","year":"1971","unstructured":"J. N. Demas and G. A. Crosby, Measurement of photoluminescence quantum yields. review, J. Phys. Chem., 1971, 75, 991\u20131024.","journal-title":"J. Phys. Chem."},{"key":"17040474_CR31","doi-asserted-by":"publisher","first-page":"7352","DOI":"10.1364\/AO.37.007352","volume":"37","author":"M Albota","year":"1998","unstructured":"M. Albota, C. Xu and W. W. Webb, Two-photon fluorescence excitation cross sections of biomolecular probes from 690 to 960 nm, Appl. Opt., 1998, 37, 7352\u20137356.","journal-title":"Appl. Opt."},{"key":"17040474_CR32","doi-asserted-by":"publisher","first-page":"235","DOI":"10.1016\/S0030-4018(01)01121-X","volume":"191","author":"D A Oulianov","year":"2001","unstructured":"D. A. Oulianov, I. V. Tomov, A. S. Dvornikov and P. M. Rentzepis, Observations on the measurement of two-photon absorption cross-section, Opt. Commun., 2001, 191, 235\u2013243.","journal-title":"Opt. Commun."},{"key":"17040474_CR33","doi-asserted-by":"publisher","first-page":"82","DOI":"10.1016\/j.molstruc.2006.09.007","volume":"833","author":"B Liu","year":"2007","unstructured":"B. Liu, J. Liu, H. Q. Wang, Y. D. Zhao and Z. L. Huang, Synthesis and spectroscopic properties of symmetrically substituted two-photon absorbing molecules with rigid elongated n-conjugation, J. Mol. Struct., 2007, 833, 82\u201387.","journal-title":"J. Mol. Struct."},{"key":"17040474_CR34","doi-asserted-by":"publisher","first-page":"481","DOI":"10.1364\/JOSAB.13.000481","volume":"13","author":"C Xu","year":"1996","unstructured":"C. Xu and W. W. Webb, Measurement of two-photon excitation cross sections of molecular fluorophores with data from 690 to 1050 nm, J. Opt. Soc. Am. B, 1996, 13, 481\u2013491.","journal-title":"J. Opt. Soc. Am. B"}],"container-title":["Photochemical &amp; Photobiological Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1039\/c8pp00071a.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1039\/c8pp00071a\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/pubs.rsc.org\/en\/content\/articlepdf\/2018\/PP\/C8PP00071A","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1039\/c8pp00071a.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,11,2]],"date-time":"2022-11-02T15:24:23Z","timestamp":1667402663000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1039\/c8pp00071a"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,4]]},"references-count":34,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2018,4]]}},"alternative-id":["17040474"],"URL":"https:\/\/doi.org\/10.1039\/c8pp00071a","relation":{},"ISSN":["1474-905X","1474-9092"],"issn-type":[{"value":"1474-905X","type":"print"},{"value":"1474-9092","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,4]]},"assertion":[{"value":"16 February 2018","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"6 March 2018","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"27 October 2020","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}]}}