{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,5]],"date-time":"2026-07-05T18:17:34Z","timestamp":1783275454248,"version":"3.54.6"},"reference-count":47,"publisher":"Royal Society of Chemistry (RSC)","issue":"19","license":[{"start":{"date-parts":[[2020,8,8]],"date-time":"2020-08-08T00:00:00Z","timestamp":1596844800000},"content-version":"am","delay-in-days":585,"URL":"http:\/\/rsc.li\/journals-terms-of-use"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Basic Research Program of China","doi-asserted-by":"crossref","award":["2016YFA0201204"],"award-info":[{"award-number":["2016YFA0201204"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["61871403"],"award-info":[{"award-number":["61871403"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81673390"],"award-info":[{"award-number":["81673390"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81603219"],"award-info":[{"award-number":["81603219"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100013058","name":"Jiangsu Provincial Key Research and Development Program","doi-asserted-by":"crossref","award":["BE2016745"],"award-info":[{"award-number":["BE2016745"]}],"id":[{"id":"10.13039\/501100013058","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100010014","name":"Six Talent Peaks Project in Jiangsu Province","doi-asserted-by":"crossref","award":["2015-WSN-085"],"award-info":[{"award-number":["2015-WSN-085"]}],"id":[{"id":"10.13039\/501100010014","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100013059","name":"Jiangsu Provincial Medical Youth Talent","doi-asserted-by":"crossref","award":["QNRC2016889"],"award-info":[{"award-number":["QNRC2016889"]}],"id":[{"id":"10.13039\/501100013059","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Double First Class University Plan","award":["CPU2018GY05"],"award-info":[{"award-number":["CPU2018GY05"]}]},{"name":"Double First Class University Plan","award":["CPU2018GY34"],"award-info":[{"award-number":["CPU2018GY34"]}]}],"content-domain":{"domain":["rsc.org"],"crossmark-restriction":true},"short-container-title":["Analyst"],"abstract":"<p>Absolute quantification of HBV-DNA by sequence-encoded Quantitative Invader assay in a single tube without using calibration curves.<\/p>","DOI":"10.1039\/c9an00970a","type":"journal-article","created":{"date-parts":[[2019,8,8]],"date-time":"2019-08-08T02:15:45Z","timestamp":1565230545000},"page":"5775-5784","update-policy":"https:\/\/doi.org\/10.1039\/rsc_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Sequence-encoded quantitative invader assay enables highly sensitive hepatitis B virus DNA quantification in a single tube without the use of a calibration curve"],"prefix":"10.1039","volume":"144","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6891-8438","authenticated-orcid":false,"given":"Nan","family":"Sheng","sequence":"first","affiliation":[{"name":"School of Life Science and Technology"},{"name":"China Pharmaceutical University"},{"name":"Nanjing 210009"},{"name":"China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1056-9156","authenticated-orcid":false,"given":"Bingjie","family":"Zou","sequence":"additional","affiliation":[{"name":"Department of Pharmacology"},{"name":"Jinling Hospital"},{"name":"Medical School of Nanjing University"},{"name":"Nanjing 210002"},{"name":"China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huan","family":"Tong","sequence":"additional","affiliation":[{"name":"Department of Pharmacology"},{"name":"Jinling Hospital"},{"name":"Medical School of Nanjing University"},{"name":"Nanjing 210002"},{"name":"China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Pharmacology"},{"name":"Jinling Hospital"},{"name":"Medical School of Nanjing University"},{"name":"Nanjing 210002"},{"name":"China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sixi","family":"Xing","sequence":"additional","affiliation":[{"name":"Key Laboratory of Drug Quality Control and Pharmacovigilance"},{"name":"Ministry of Education"},{"name":"School of Pharmacy"},{"name":"China Pharmaceutical University"},{"name":"Nanjing 210009"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3452-6396","authenticated-orcid":false,"given":"Qinxin","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Pharmacology"},{"name":"Jinling Hospital"},{"name":"Medical School of Nanjing University"},{"name":"Nanjing 210002"},{"name":"China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6675-4452","authenticated-orcid":false,"given":"Guohua","family":"Zhou","sequence":"additional","affiliation":[{"name":"School of Life Science and Technology"},{"name":"China Pharmaceutical University"},{"name":"Nanjing 210009"},{"name":"China"},{"name":"Department of Pharmacology"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"292","published-online":{"date-parts":[[2019]]},"reference":[{"key":"C9AN00970A-(cit1)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"1221","DOI":"10.1126\/science.1243462","volume":"343","author":"Lucifora","year":"2014","journal-title":"Science"},{"key":"C9AN00970A-(cit2)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"e10","DOI":"10.1016\/S1473-3099(15)00436-3","volume":"16","author":"Brahmania","year":"2016","journal-title":"Lancet Infect. Dis."},{"key":"C9AN00970A-(cit3)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1016\/j.tim.2018.01.009","volume":"26","author":"Shih","year":"2018","journal-title":"Trends Microbiol."},{"key":"C9AN00970A-(cit4)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"1810","DOI":"10.1136\/gutjnl-2014-308211","volume":"64","author":"Giles","year":"2015","journal-title":"Gut"},{"key":"C9AN00970A-(cit5)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1053\/j.gastro.2014.10.004","volume":"148","author":"Phillips","year":"2015","journal-title":"Gastroenterology"},{"issue":"Suppl 4","key":"C9AN00970A-(cit6)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"S298","DOI":"10.1093\/cid\/ciw024","volume":"62","author":"Wong","year":"2016","journal-title":"Clin. Infect. Dis."},{"key":"C9AN00970A-(cit7)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"1802","DOI":"10.1001\/jama.2018.3795","volume":"319","author":"Tang","year":"2018","journal-title":"J. Am. Med. Assoc."},{"key":"C9AN00970A-(cit8)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"S166","DOI":"10.1002\/hep.22899","volume":"49","author":"Andersson","year":"2009","journal-title":"Hepatology"},{"key":"C9AN00970A-(cit9)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1002\/hep.26686","volume":"59","author":"Kitrinos","year":"2014","journal-title":"Hepatology"},{"key":"C9AN00970A-(cit10)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"652","DOI":"10.1053\/j.gastro.2017.10.017","volume":"154","author":"Klumpp","year":"2018","journal-title":"Gastroenterology"},{"key":"C9AN00970A-(cit11)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"2313","DOI":"10.1016\/S0140-6736(18)31865-8","volume":"392","author":"Seto","year":"2018","journal-title":"Lancet"},{"key":"C9AN00970A-(cit12)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1038\/nmeth713","volume":"1","author":"Shi","year":"2004","journal-title":"Nat. Methods"},{"key":"C9AN00970A-(cit13)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"449","DOI":"10.3233\/JAD-2011-111337","volume":"29","author":"Tharp","year":"2012","journal-title":"J. Alzheimer's Dis."},{"key":"C9AN00970A-(cit14)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"817","DOI":"10.1093\/cid\/civ1214","volume":"62","author":"Gadsby","year":"2016","journal-title":"Clin. Infect. Dis."},{"key":"C9AN00970A-(cit15)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"3325","DOI":"10.1128\/JCM.00024-06","volume":"44","author":"Welzel","year":"2006","journal-title":"J. Clin. Microbiol."},{"key":"C9AN00970A-(cit16)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/j.jviromet.2018.03.001","volume":"256","author":"Portilho","year":"2018","journal-title":"J. Virol. Methods"},{"key":"C9AN00970A-(cit17)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1016\/j.cca.2014.07.021","volume":"437","author":"Wang","year":"2014","journal-title":"Clin. Chim. Acta"},{"key":"C9AN00970A-(cit18)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"e126","DOI":"10.1093\/nar\/gku603","volume":"42","author":"Mojtahedi","year":"2014","journal-title":"Nucleic Acids Res."},{"key":"C9AN00970A-(cit19)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"9557","DOI":"10.1002\/anie.201602763","volume":"55","author":"Schoepp","year":"2016","journal-title":"Angew. Chem., Int. Ed."},{"key":"C9AN00970A-(cit20)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"2132","DOI":"10.1021\/acs.analchem.5b03692","volume":"88","author":"Kline","year":"2016","journal-title":"Anal. Chem."},{"key":"C9AN00970A-(cit21)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1002\/1878-0261.12037","volume":"11","author":"van Dessel","year":"2017","journal-title":"Mol. Oncol."},{"key":"C9AN00970A-(cit22)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"4437","DOI":"10.1158\/1078-0432.CCR-18-0143","volume":"24","author":"Hu","year":"2018","journal-title":"Clin. Cancer Res."},{"key":"C9AN00970A-(cit23)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"6474","DOI":"10.1021\/ac103230c","volume":"83","author":"Sanders","year":"2011","journal-title":"Anal. Chem."},{"key":"C9AN00970A-(cit24)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"724","DOI":"10.1373\/clinchem.2014.236208","volume":"61","author":"Zhou","year":"2015","journal-title":"Clin. Chem."},{"key":"C9AN00970A-(cit25)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1016\/j.bios.2016.02.039","volume":"80","author":"Xu","year":"2016","journal-title":"Biosens. Bioelectron."},{"key":"C9AN00970A-(cit26)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1186\/s13024-016-0069-4","volume":"11","author":"Zhong","year":"2016","journal-title":"Mol. Neurodegener."},{"key":"C9AN00970A-(cit27)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"2297","DOI":"10.1159\/000484380","volume":"43","author":"Scantamburlo","year":"2017","journal-title":"Cell. Physiol. Biochem."},{"key":"C9AN00970A-(cit28)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1016\/j.tibtech.2016.09.003","volume":"35","author":"Kuang","year":"2017","journal-title":"Trends Biotechnol."},{"key":"C9AN00970A-(cit29)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.aca.2018.07.064","volume":"1040","author":"Qian","year":"2018","journal-title":"Anal. Chim. Acta"},{"key":"C9AN00970A-(cit30)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1038\/7044","volume":"17","author":"Lyamichev","year":"1999","journal-title":"Nat. Biotechnol."},{"key":"C9AN00970A-(cit31)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1002\/hep.20353","volume":"40","author":"Wong","year":"2004","journal-title":"Hepatology"},{"key":"C9AN00970A-(cit32)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"13722","DOI":"10.1039\/C4CC06079B","volume":"50","author":"Zou","year":"2014","journal-title":"Chem. Commun."},{"key":"C9AN00970A-(cit33)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1016\/j.jhep.2006.05.014","volume":"45","author":"Wong","year":"2006","journal-title":"J. Hepatol."},{"key":"C9AN00970A-(cit34)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"3443","DOI":"10.1128\/JCM.01175-06","volume":"44","author":"Allawi","year":"2006","journal-title":"J. Clin. Microbiol."},{"key":"C9AN00970A-(cit35)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.jviromet.2006.07.014","volume":"138","author":"Tadokoro","year":"2006","journal-title":"J. Virol. Methods"},{"key":"C9AN00970A-(cit36)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.jviromet.2010.10.001","volume":"171","author":"Tadokoro","year":"2011","journal-title":"J. Virol. Methods"},{"key":"C9AN00970A-(cit37)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.micres.2008.06.001","volume":"165","author":"Tadokoro","year":"2010","journal-title":"Microbiol. Res."},{"key":"C9AN00970A-(cit38)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/j.jviromet.2012.10.011","volume":"189","author":"Tadokoro","year":"2013","journal-title":"J. Virol. Methods"},{"key":"C9AN00970A-(cit39)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"852","DOI":"10.1373\/clinchem.2016.263996","volume":"63","author":"Wang","year":"2017","journal-title":"Clin. Chem."},{"key":"C9AN00970A-(cit40)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"1666","DOI":"10.1039\/C7SC04210H","volume":"9","author":"Liu","year":"2018","journal-title":"Chem. Sci."},{"key":"C9AN00970A-(cit41)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1002\/rmv.400","volume":"14","author":"Bartholomeusz","year":"2004","journal-title":"Rev. Med. Virol."},{"key":"C9AN00970A-(cit42)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"S4","DOI":"10.1016\/j.jhep.2016.01.027","volume":"64","author":"Tong","year":"2016","journal-title":"J. Hepatol."},{"key":"C9AN00970A-(cit43)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.virol.2016.01.029","volume":"492","author":"Prakash","year":"2016","journal-title":"Virology"},{"key":"C9AN00970A-(cit44)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1002\/hep.24363","volume":"54","author":"Hsu","year":"2011","journal-title":"Hepatology"},{"key":"C9AN00970A-(cit45)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"3641","DOI":"10.1128\/JCM.01306-10","volume":"48","author":"Chevaliez","year":"2010","journal-title":"J. Clin. Microbiol."},{"key":"C9AN00970A-(cit46)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.jcv.2018.01.012","volume":"101","author":"Shin","year":"2018","journal-title":"J. Clin. Virol."},{"key":"C9AN00970A-(cit47)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"7395","DOI":"10.1002\/anie.201001529","volume":"50","author":"Zou","year":"2011","journal-title":"Angew. Chem., Int. Ed."}],"container-title":["The Analyst"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/pubs.rsc.org\/en\/content\/articlepdf\/2019\/AN\/C9AN00970A","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,5]],"date-time":"2026-07-05T18:10:24Z","timestamp":1783275024000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.rsc.org\/an\/article\/144\/19\/5775-5784\/631563"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019]]},"references-count":47,"journal-issue":{"issue":"19","published-print":{"date-parts":[[2019,9,23]]}},"URL":"https:\/\/doi.org\/10.1039\/c9an00970a","relation":{},"ISSN":["0003-2654","1364-5528"],"issn-type":[{"value":"0003-2654","type":"print"},{"value":"1364-5528","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019]]},"assertion":[{"value":"This document is Similarity Check deposited","name":"similarity_check","explanation":{"URL":"https:\/\/www.crossref.org\/services\/similarity-check\/"},"group":{"name":"similarity_check","label":"SIMILARITY CHECK"}},{"value":"Supplementary Information","URL":"https:\/\/doi.org\/10.1039\/C9AN00970A","name":"related_data","group":{"name":"related_data","label":"RELATED DATA"}},{"value":"Nan Sheng (ORCID)","URL":"http:\/\/orcid.org\/0000-0002-6891-8438","name":"identifier","group":{"name":"author_identifiers","label":"IDENTIFIERS"}},{"value":"Bingjie Zou (ORCID)","URL":"http:\/\/orcid.org\/0000-0003-1056-9156","name":"identifier","group":{"name":"author_identifiers","label":"IDENTIFIERS"}},{"value":"Qinxin Song (ORCID)","URL":"http:\/\/orcid.org\/0000-0003-3452-6396","name":"identifier","group":{"name":"author_identifiers","label":"IDENTIFIERS"}},{"value":"Guohua Zhou (ORCID)","URL":"http:\/\/orcid.org\/0000-0001-6675-4452","name":"identifier","group":{"name":"author_identifiers","label":"IDENTIFIERS"}},{"value":"Single-blind","order":2,"name":"peer_review_method","group":{"name":"peer_review_method","label":"PEER REVIEW METHOD"}},{"value":"Received 27 May 2019; Accepted 6 August 2019;  Accepted Manuscript published 8 August 2019;  Advance Article published 28 August 2019;  Version of Record published 23 September 2019","order":1,"name":"history","group":{"name":"publication_history","label":"PUBLICATION HISTORY"}}]}}