{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T15:02:56Z","timestamp":1790521376555,"version":"4.1.0"},"reference-count":38,"publisher":"Royal Society of Chemistry (RSC)","issue":"19","license":[{"start":{"date-parts":[[2021,4,7]],"date-time":"2021-04-07T00:00:00Z","timestamp":1617753600000},"content-version":"am","delay-in-days":462,"URL":"http:\/\/rsc.li\/journals-terms-of-use"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["21607150"],"award-info":[{"award-number":["21607150"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["21607152"],"award-info":[{"award-number":["21607152"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["21876171"],"award-info":[{"award-number":["21876171"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100012166","name":"National Basic Research Program of China","doi-asserted-by":"crossref","award":["2017YFC1600200"],"award-info":[{"award-number":["2017YFC1600200"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["rsc.org"],"crossmark-restriction":true},"short-container-title":["Anal. Methods"],"abstract":"<p>We compare the efficiencies of different cell harvesting methods and metabolite extraction methods in sample preparation procedures and provide a cell sample processing protocol which focuses on maximizing metabolite recovery ranging from polar to lipidic ones.<\/p>","DOI":"10.1039\/c9ay02753j","type":"journal-article","created":{"date-parts":[[2020,4,6]],"date-time":"2020-04-06T20:43:01Z","timestamp":1586205781000},"page":"2491-2498","update-policy":"https:\/\/doi.org\/10.1039\/rsc_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Effects of harvesting and extraction methods on metabolite recovery from adherently growing mammalian cells"],"prefix":"10.1039","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4416-5566","authenticated-orcid":false,"given":"Yun","family":"Luo","sequence":"first","affiliation":[{"name":"CAS Key Laboratory of Separation Sciences for Analytical Chemistry"},{"name":"Dalian Institute of Chemical Physics"},{"name":"Chinese Academy of Sciences"},{"name":"Dalian"},{"name":"China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ningbo","family":"Geng","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Separation Sciences for Analytical Chemistry"},{"name":"Dalian Institute of Chemical Physics"},{"name":"Chinese Academy of Sciences"},{"name":"Dalian"},{"name":"China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0790-5219","authenticated-orcid":false,"given":"Baoqin","family":"Zhang","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Separation Sciences for Analytical Chemistry"},{"name":"Dalian Institute of Chemical Physics"},{"name":"Chinese Academy of Sciences"},{"name":"Dalian"},{"name":"China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiping","family":"Chen","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Separation Sciences for Analytical Chemistry"},{"name":"Dalian Institute of Chemical Physics"},{"name":"Chinese Academy of Sciences"},{"name":"Dalian"},{"name":"China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haijun","family":"Zhang","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Separation Sciences for Analytical Chemistry"},{"name":"Dalian Institute of Chemical Physics"},{"name":"Chinese Academy of Sciences"},{"name":"Dalian"},{"name":"China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"292","published-online":{"date-parts":[[2020]]},"reference":[{"key":"C9AY02753J-(cit1)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"2762","DOI":"10.1002\/elps.201200605","volume":"34","author":"Leon","year":"2013","journal-title":"Electrophoresis"},{"key":"C9AY02753J-(cit2)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.ab.2016.11.009","volume":"517","author":"Zhang","year":"2017","journal-title":"Anal. Biochem."},{"key":"C9AY02753J-(cit3)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.ab.2010.04.013","volume":"404","author":"Danielsson","year":"2010","journal-title":"Anal. Biochem."},{"key":"C9AY02753J-(cit4)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"8864","DOI":"10.1038\/s41598-017-08433-9","volume":"7","author":"Kim","year":"2017","journal-title":"Sci. Rep."},{"key":"C9AY02753J-(cit5)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1080\/14737159.2019.1607729","volume":"19","author":"Lucarelli","year":"2019","journal-title":"Expert Rev. Mol. Diagn."},{"key":"C9AY02753J-(cit6)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1039\/C1AN15605E","volume":"137","author":"Zhang","year":"2012","journal-title":"Analyst"},{"key":"C9AY02753J-(cit7)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"30","DOI":"10.3389\/fmolb.2016.00030","volume":"3","author":"Grissa","year":"2016","journal-title":"Front. Mol. Biosci."},{"key":"C9AY02753J-(cit8)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"10720","DOI":"10.1021\/es203068f","volume":"45","author":"Lai","year":"2011","journal-title":"Environ. Sci. Technol."},{"key":"C9AY02753J-(cit9)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.aca.2017.03.004","volume":"966","author":"Wang","year":"2017","journal-title":"Anal. Chim. Acta"},{"key":"C9AY02753J-(cit10)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"6362","DOI":"10.1039\/C6AN01753C","volume":"141","author":"Zhou","year":"2016","journal-title":"Analyst"},{"key":"C9AY02753J-(cit11)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.aca.2016.05.008","volume":"927","author":"Wang","year":"2016","journal-title":"Anal. Chim. Acta"},{"key":"C9AY02753J-(cit12)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1016\/j.trac.2015.08.003","volume":"75","author":"Madji Hounoum","year":"2016","journal-title":"TrAC, Trends Anal. Chem."},{"key":"C9AY02753J-(cit13)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"1217","DOI":"10.1007\/s00216-015-9227-8","volume":"408","author":"Garc\u00eda-Ca\u00f1averas","year":"2016","journal-title":"Anal. Bioanal. Chem."},{"key":"C9AY02753J-(cit14)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.ab.2015.01.003","volume":"475","author":"Ser","year":"2015","journal-title":"Anal. Biochem."},{"key":"C9AY02753J-(cit15)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"8861","DOI":"10.1007\/s00216-015-9047-x","volume":"407","author":"Hounoum","year":"2015","journal-title":"Anal. Bioanal. Chem."},{"key":"C9AY02753J-(cit16)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"1127","DOI":"10.1007\/s00216-010-4425-x","volume":"399","author":"Dettmer","year":"2011","journal-title":"Anal. Bioanal. Chem."},{"key":"C9AY02753J-(cit17)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"3406","DOI":"10.1021\/ac103313x","volume":"83","author":"Lorenz","year":"2011","journal-title":"Anal. Chem."},{"key":"C9AY02753J-(cit18)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"4421","DOI":"10.1007\/s00018-017-2582-1","volume":"74","author":"Hayton","year":"2017","journal-title":"Cell. Mol. Life Sci."},{"key":"C9AY02753J-(cit19)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1007\/s11306-007-0080-4","volume":"3","author":"van der Werf","year":"2007","journal-title":"Metabolomics"},{"key":"C9AY02753J-(cit20)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"3014","DOI":"10.1039\/C9AY00792J","volume":"11","author":"Xu","year":"2019","journal-title":"Anal. Methods"},{"key":"C9AY02753J-(cit21)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1016\/j.ab.2007.10.037","volume":"373","author":"Ritter","year":"2008","journal-title":"Anal. Biochem."},{"key":"C9AY02753J-(cit22)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"3678","DOI":"10.1039\/C9AY00326F","volume":"11","author":"Zhang","year":"2019","journal-title":"Anal. Methods"},{"key":"C9AY02753J-(cit23)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"1953","DOI":"10.1039\/c2ay25046b","volume":"4","author":"Hutschenreuther","year":"2012","journal-title":"Anal. Methods"},{"key":"C9AY02753J-(cit24)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1007\/s11306-008-0137-z","volume":"5","author":"Teng","year":"2008","journal-title":"Metabolomics"},{"key":"C9AY02753J-(cit25)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"5279","DOI":"10.1007\/s00216-013-6927-9","volume":"405","author":"Bi","year":"2013","journal-title":"Anal. Bioanal. Chem."},{"key":"C9AY02753J-(cit26)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.jpba.2015.03.001","volume":"110","author":"Ibanez","year":"2015","journal-title":"J. Pharm. Biomed. Anal."},{"key":"C9AY02753J-(cit27)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/j.trac.2014.06.003","volume":"61","author":"Raterink","year":"2014","journal-title":"TrAC, Trends Anal. Chem."},{"key":"C9AY02753J-(cit28)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1021\/acs.analchem.5b04491","volume":"88","author":"Cajka","year":"2016","journal-title":"Anal. Chem."},{"key":"C9AY02753J-(cit29)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"7766","DOI":"10.1021\/ac901269h","volume":"84","author":"Myint","year":"2009","journal-title":"Anal. Chem."},{"key":"C9AY02753J-(cit30)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"5992","DOI":"10.1021\/ac300716u","volume":"84","author":"Whiley","year":"2012","journal-title":"Anal. Chem."},{"key":"C9AY02753J-(cit31)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.chroma.2013.05.019","volume":"1298","author":"Chen","year":"2013","journal-title":"J. Chromatogr. A"},{"key":"C9AY02753J-(cit32)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.chroma.2013.03.043","volume":"1300","author":"Yang","year":"2013","journal-title":"J. Chromatogr. A"},{"key":"C9AY02753J-(cit33)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"1197","DOI":"10.1007\/s11306-014-0658-6","volume":"10","author":"Chang","year":"2014","journal-title":"Metabolomics"},{"key":"C9AY02753J-(cit34)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"1137","DOI":"10.1194\/jlr.D700041-JLR200","volume":"49","author":"Matyash","year":"2008","journal-title":"J. Lipid Res."},{"key":"C9AY02753J-(cit35)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"2038","DOI":"10.1039\/C7AN00207F","volume":"14","author":"Kapoore","year":"2017","journal-title":"Analyst"},{"key":"C9AY02753J-(cit36)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"3145","DOI":"10.1021\/acs.est.5b03678","volume":"50","author":"Wang","year":"2016","journal-title":"Environ. Sci. Technol."},{"key":"C9AY02753J-(cit37)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s11306-016-1104-8","volume":"12","author":"Muschet","year":"2016","journal-title":"Metabolomics"},{"key":"C9AY02753J-(cit38)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"7379","DOI":"10.1021\/ac900999t","volume":"81","author":"Canelas","year":"2009","journal-title":"Anal. Chem."}],"container-title":["Analytical Methods"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/pubs.rsc.org\/en\/content\/articlepdf\/2020\/AY\/C9AY02753J","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T18:05:19Z","timestamp":1783015519000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.rsc.org\/ay\/article\/12\/19\/2491-2498\/632845"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020]]},"references-count":38,"journal-issue":{"issue":"19","published-print":{"date-parts":[[2020,5,21]]}},"URL":"https:\/\/doi.org\/10.1039\/c9ay02753j","relation":{},"ISSN":["1759-9660","1759-9679"],"issn-type":[{"value":"1759-9660","type":"print"},{"value":"1759-9679","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020]]},"assertion":[{"value":"This document is Similarity Check deposited","name":"similarity_check","explanation":{"URL":"https:\/\/www.crossref.org\/services\/similarity-check\/"},"group":{"name":"similarity_check","label":"SIMILARITY CHECK"}},{"value":"Supplementary Information","URL":"https:\/\/doi.org\/10.1039\/C9AY02753J","name":"related_data","group":{"name":"related_data","label":"RELATED DATA"}},{"value":"Yun Luo (ORCID)","URL":"http:\/\/orcid.org\/0000-0002-4416-5566","name":"identifier","group":{"name":"author_identifiers","label":"IDENTIFIERS"}},{"value":"Baoqin Zhang (ORCID)","URL":"http:\/\/orcid.org\/0000-0003-0790-5219","name":"identifier","group":{"name":"author_identifiers","label":"IDENTIFIERS"}},{"value":"Single-blind","order":2,"name":"peer_review_method","group":{"name":"peer_review_method","label":"PEER REVIEW METHOD"}},{"value":"Received 24 December 2019; Accepted 3 April 2020; Accepted Manuscript published 7 April 2020; Advance Article published 6 May 2020; Version of Record published 21 May 2020","order":1,"name":"history","group":{"name":"publication_history","label":"PUBLICATION HISTORY"}}]}}