{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T01:45:21Z","timestamp":1776217521336,"version":"3.50.1"},"reference-count":44,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2020,12,7]],"date-time":"2020-12-07T00:00:00Z","timestamp":1607299200000},"content-version":"vor","delay-in-days":463,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"},{"start":{"date-parts":[[2020,9,4]],"date-time":"2020-09-04T00:00:00Z","timestamp":1599177600000},"content-version":"am","delay-in-days":369,"URL":"http:\/\/rsc.li\/journals-terms-of-use"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2013\/50218-2"],"award-info":[{"award-number":["2013\/50218-2"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","award":["2013\/50218-2"],"award-info":[{"award-number":["2013\/50218-2"]}]}],"content-domain":{"domain":["rsc.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2019,9,18]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Proteomic analysis of the fungus Aspergillus niger showed that its capacity to absorb metals was boosted by physiological modification under metal stress conditions. To investigate the proteome elicited by copper stress, the mine-isolated strain A. niger IOC 4687 was cultured in the absence (control) or presence of copper ions (50 mg L\u22121) for 72 h. Protein extract from each treatment was analyzed by nano-liquid chromatography-mass spectrometry and proteins were identified using PEAKS Studio 8.5 software. Grouping proteins by functional category showed that antioxidant enzymes, such as catalase, superoxide dismutase, and cytochrome c peroxidase, were present in both treatments. However, heat shock proteins (Hsp60 and Hsp70) and some metalloproteins (LMBR1 domain protein and A. niger contig An09c0040) were only observed after copper treatment. These proteins were the cellular response to the stress conditions. In conclusion, significant changes in the proteome of A. niger were observed due to the presence of copper.<\/jats:p>","DOI":"10.1039\/c9mt00137a","type":"journal-article","created":{"date-parts":[[2019,9,4]],"date-time":"2019-09-04T08:58:53Z","timestamp":1567587533000},"page":"1558-1566","update-policy":"https:\/\/doi.org\/10.1039\/rsc_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Response mechanism of mine-isolated fungus\n                    <i>Aspergillus niger<\/i>\n                    IOC 4687 to copper stress determined by proteomics"],"prefix":"10.1093","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2600-4582","authenticated-orcid":false,"given":"Meriellen","family":"Dias","sequence":"first","affiliation":[{"name":"Dempster MS Lab \u2013 Chemical Engineering Department of Polytechnic School of University of S\u00e3o Paulo , Rua do Lago, 250, Bloco B 3 andar, S\u00e3o Paulo-SP, 05508-080,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Thalles Jocelino","family":"Gomes de Lacerda","sequence":"additional","affiliation":[{"name":"Federal University of S\u00e3o Paulo , Rua Tres de Maio, 100, 2 andar, S\u00e3o Paulo-SP, 05508-080,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5038-4597","authenticated-orcid":false,"given":"Silas","family":"Perdig\u00e3o Cota de Almeida","sequence":"additional","affiliation":[{"name":"Dempster MS Lab \u2013 Chemical Engineering Department of Polytechnic School of University of S\u00e3o Paulo , Rua do Lago, 250, Bloco B 3 andar, S\u00e3o Paulo-SP, 05508-080,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4356-7077","authenticated-orcid":false,"given":"Lidiane Maria","family":"de Andrade","sequence":"additional","affiliation":[{"name":"Dempster MS Lab \u2013 Chemical Engineering Department of Polytechnic School of University of S\u00e3o Paulo , Rua do Lago, 250, Bloco B 3 andar, S\u00e3o Paulo-SP, 05508-080,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudio Augusto","family":"Oller do Nascimento","sequence":"additional","affiliation":[{"name":"Dempster MS Lab \u2013 Chemical Engineering Department of Polytechnic School of University of S\u00e3o Paulo , Rua do Lago, 250, Bloco B 3 andar, S\u00e3o Paulo-SP, 05508-080,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4840-8315","authenticated-orcid":false,"given":"Enrique Eduardo","family":"Rozas","sequence":"additional","affiliation":[{"name":"Dempster MS Lab \u2013 Chemical Engineering Department of Polytechnic School of University of S\u00e3o Paulo , Rua do Lago, 250, Bloco B 3 andar, S\u00e3o Paulo-SP, 05508-080,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2078-9286","authenticated-orcid":false,"given":"Maria Anita","family":"Mendes","sequence":"additional","affiliation":[{"name":"Dempster MS Lab \u2013 Chemical Engineering Department of Polytechnic School of University of S\u00e3o Paulo , Rua do Lago, 250, Bloco B 3 andar, S\u00e3o Paulo-SP, 05508-080,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2019,9,4]]},"reference":[{"key":"2026041420234979200_cit1","doi-asserted-by":"crossref","first-page":"953","DOI":"10.1016\/S0032-9592(02)00235-2","article-title":"Standardization of heavy metal biosorption tests: equilibrium and modeling study","volume":"38","author":"Veglio","year":"2003","journal-title":"Process Biochem."},{"key":"2026041420234979200_cit2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jhazmat.2007.09.101","article-title":"Novel biofiltration methods for the treatment of heavy metals from industrial wastewater","volume":"151","author":"Srivastava","year":"2008","journal-title":"J. Hazard. Mater."},{"key":"2026041420234979200_cit3","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1016\/j.jcis.2006.05.032","article-title":"Adsorbent based on silkworm chrysalides for removal of heavy metals from wastewaters","volume":"301","author":"Paulino","year":"2006","journal-title":"J. Colloid Interface Sci."},{"key":"2026041420234979200_cit4","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1016\/j.chemosphere.2014.08.057","article-title":"Microfungi in highly copper-contaminated soils from an abandoned Fe\u2013Cu sulphide mine: growth responses, tolerance and bioaccumulation","volume":"117","author":"Zotti","year":"2014","journal-title":"Chemosphere"},{"key":"2026041420234979200_cit5","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1038\/nrmicro2519","article-title":"Untapped potential: exploiting fungi in bioremediation of hazardous chemicals","volume":"9","author":"Harms","year":"2011","journal-title":"Nat. Rev. Microbiol."},{"key":"2026041420234979200_cit6","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.biortech.2013.12.102","article-title":"Biosorption: current perspectives on concept, definition, and application","volume":"160","author":"Gadd","year":"2014","journal-title":"Bioresour. Technol."},{"key":"2026041420234979200_cit7","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/S0960-8524(99)00144-3","article-title":"Review: Factors limiting bioremediation technologies","volume":"74","author":"Boopathy","year":"2000","journal-title":"Bioresour. Technol."},{"key":"2026041420234979200_cit8","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1641\/0006-3568(2005)055[0273:TYACBI]2.0.CO;2","article-title":"Thirty years and counting: Bioremediation in its prime","volume":"55","author":"Litchfield","year":"2005","journal-title":"Bioscience"},{"key":"2026041420234979200_cit9","doi-asserted-by":"crossref","first-page":"2766","DOI":"10.1016\/j.biortech.2007.06.036","article-title":"Batch and column studies of biosorption of heavy metals by Caulerpa lentillifera","volume":"99","author":"Apiratikul","year":"2008","journal-title":"Bioresour. Technol."},{"key":"2026041420234979200_cit10","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1016\/j.jenvman.2010.11.011","article-title":"Removal of heavy metal ions from wastewater: A Review","volume":"92","author":"Fu","year":"2011","journal-title":"J. Environ. Manage."},{"key":"2026041420234979200_cit11","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1016\/j.copbio.2016.01.013","article-title":"Microbial communities, processes, and functions in acid mine drainage ecosystems","volume":"38","author":"Chen","year":"2016","journal-title":"Curr. Opin. Biotechnol."},{"key":"2026041420234979200_cit12","doi-asserted-by":"crossref","first-page":"883","DOI":"10.1016\/j.watres.2008.11.046","article-title":"Occurrence and role of algae and fungi in acid mine drainage environment with special reference to metals and sulfate immobilization","volume":"43","author":"Das","year":"2009","journal-title":"Water Res."},{"key":"2026041420234979200_cit13","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1016\/j.jhazmat.2006.11.011","article-title":"Chromium (VI) biosorption by dried Rhizopus arrhizus: effect of salt (NaCl) concentration on equilibrium and kinetic parameters","volume":"145","author":"Aksu","year":"2007","journal-title":"J. Hazard. Mater."},{"key":"2026041420234979200_cit14","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.colsurfa.2008.11.053","article-title":"Statistical modeling and optimization of the cadmium biosorption process in an aqueous solution using Aspergillus niger","volume":"337","author":"Amini","year":"2009","journal-title":"Colloids Surf."},{"key":"2026041420234979200_cit15","doi-asserted-by":"crossref","first-page":"1727","DOI":"10.1016\/j.biortech.2009.10.012","article-title":"Biosorption of copper (II) and cadmium (II) from aqueous solutions by free and immobilized biomass of Aspergillus niger","volume":"101","author":"Tsekova","year":"2010","journal-title":"Bioresour. Technol."},{"key":"2026041420234979200_cit16","doi-asserted-by":"crossref","first-page":"3829","DOI":"10.1016\/j.biortech.2007.07.032","article-title":"Removal of copper ions by the filamentous fungus, Rhizopus oryzae from aqueous solution","volume":"99","author":"Bhainsa","year":"2008","journal-title":"Bioresour. Technol."},{"key":"2026041420234979200_cit17","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1016\/j.ejbt.2015.09.006","article-title":"Copper-induced adaptation, oxidative stress and its tolerance in Aspergillus niger UCP1261","volume":"18","author":"Luna","year":"2015","journal-title":"Electron. J. Biotechnol."},{"key":"2026041420234979200_cit18","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1016\/j.jbiotec.2009.12.018","article-title":"Biotechnological applications of microbial proteomes","volume":"145","author":"Han","year":"2010","journal-title":"J. Biotechnol."},{"key":"2026041420234979200_cit19","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1590\/S0036-46652013000200009","article-title":"The copper interference with the melanogenesis of Cryptococcus neoformans","volume":"55","author":"Mauch","year":"2013","journal-title":"Rev. Inst. Med. Trop. Sao Paulo"},{"key":"2026041420234979200_cit20","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.fgb.2014.09.008","article-title":"Identification of high-affinity copper transporters in Aspergillus fumigates","volume":"73","author":"Park","year":"2014","journal-title":"Fungal Genet. Biol."},{"key":"2026041420234979200_cit21","doi-asserted-by":"crossref","first-page":"1228","DOI":"10.1002\/rcm.7555","article-title":"Identification of microorganisms in biofluids of individuals with periodontitis and chronic kidney disease using matrix-assisted laser desorption\/ionization time-of-flight mass spectrometry","volume":"20","author":"Alves","year":"2016","journal-title":"Rapid Commun. Mass Spectrom."},{"key":"2026041420234979200_cit22","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1016\/S0167-7799(97)01136-0","article-title":"On-line and in situ monitoring of biomass in submerged cultivations","volume":"15","author":"Olsson","year":"1977","journal-title":"Trends Biotechnol."},{"key":"2026041420234979200_cit23","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1111\/1567-1364.12073","article-title":"Evolutionary engineering and transcriptomic analysis of nickel-resistance Saccharomyces cerevisae","volume":"13","author":"K\u00fc\u00e7\u00fckg\u00f6ze","year":"2013","journal-title":"FEMS Yeast Res."},{"key":"2026041420234979200_cit24","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1074\/mcp.M111.010587","article-title":"PEAKS DB: De Novo sequencing assisted database to search for sensitive and accurate peptide identification","volume":"11","author":"Zhang","year":"2012","journal-title":"Mol. Cell. Proteomics"},{"key":"2026041420234979200_cit25","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1039\/B603084J","article-title":"Natural products of filamentous fungi: enzymes, genes, and their regulation","volume":"24","author":"Hoffmeister","year":"2007","journal-title":"Nat. Prod. Rep."},{"key":"2026041420234979200_cit26"},{"key":"2026041420234979200_cit27","doi-asserted-by":"crossref","first-page":"2522","DOI":"10.1016\/j.jprot.2011.04.014","article-title":"Copper exposure effects on yeast mitochondrial proteome","volume":"74","author":"Banci","year":"2011","journal-title":"J. Proteomics"},{"key":"2026041420234979200_cit28","first-page":"657","article-title":"Cellular response to Cu and Zn induced oxidative stress in Aspergillus fumigatus isolated from polluted soils in Bulgaria","volume":"44","author":"Krumova","year":"2016","journal-title":"Clean: Soil, Air, Water"},{"key":"2026041420234979200_cit29","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1007\/s11802-008-0060-y","article-title":"Heavy metal bioaccumulation and toxicity with special reference to microalgae","volume":"7","author":"Urunakumara","year":"2008","journal-title":"J. Ocean Univ. China"},{"key":"2026041420234979200_cit30","doi-asserted-by":"crossref","first-page":"1460","DOI":"10.1007\/s00284-011-9881-9","article-title":"Effects of copper exposure on expression of glutathione-related genes in Acidithiobacillus ferrooxidans","volume":"62","author":"Xia","year":"2011","journal-title":"Curr. Microbiol."},{"key":"2026041420234979200_cit31","doi-asserted-by":"crossref","first-page":"e00485","DOI":"10.1002\/mbo3.485","article-title":"A proteomic based investigation of potential copper responsive biomarkers: Proteins, conceptual networks, and metabolic pathways featuring Penicillium janthinellum from a heavy metal polluted ecological niche","volume":"6","author":"Feng","year":"2017","journal-title":"MicrobiologyOpen"},{"key":"2026041420234979200_cit32","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/S1367-5931(02)00298-3","article-title":"Molecular Mechanisms of copper uptake and distribution","volume":"6","author":"Puig","year":"2002","journal-title":"Curr. Opin. Chem. Biol."},{"key":"2026041420234979200_cit33","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1111\/mmi.12653","article-title":"Iron and copper as virulence modulators in human fungal pathogens","volume":"93","author":"Ding","year":"2014","journal-title":"Mol. Microbiol."},{"key":"2026041420234979200_cit34","doi-asserted-by":"crossref","first-page":"18954","DOI":"10.1074\/jbc.R115.647099","article-title":"The role of copper and zinc toxicity in immune defense against bacterial pathogens","volume":"290","author":"Djoko","year":"2015","journal-title":"J. Biol. Chem."},{"key":"2026041420234979200_cit35","doi-asserted-by":"crossref","first-page":"36252","DOI":"10.1074\/jbc.274.51.36252","article-title":"A copper-sensing transcription factor regulates iron uptake genes in Schizosaccharomyces pombe","volume":"274","author":"Labb\u00e9","year":"1999","journal-title":"J. Biol. Chem."},{"key":"2026041420234979200_cit36","doi-asserted-by":"crossref","first-page":"22950","DOI":"10.1074\/jbc.M202682200","article-title":"Fep1, an iron sensor regulating iron transporter gene expression in Schizosaccharomyces pombe","volume":"277","author":"Pelletier","year":"2002","journal-title":"J. Biol. Chem."},{"key":"2026041420234979200_cit37","doi-asserted-by":"crossref","first-page":"2174","DOI":"10.1016\/j.celrep.2017.05.075","article-title":"Aspergillus fumigatus copper export machinery and reactive oxygen intermediate defense counter host copper-mediated oxidative antimicrobial offense","volume":"19","author":"Wiemann","year":"2017","journal-title":"Cell Rep."},{"key":"2026041420234979200_cit38","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1038\/nature08300","article-title":"Metalloproteins and metal sensing","volume":"460","author":"Waldron","year":"2009","journal-title":"Nature"},{"key":"2026041420234979200_cit39","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1007\/s10534-012-9531-0","article-title":"Proteomic study of the yeast Rhodotorula mucilaginosa RCL-11 under copper stress","volume":"25","author":"Irazusta","year":"2012","journal-title":"Biometals"},{"key":"2026041420234979200_cit40","first-page":"394","article-title":"Molecular detection and characterization of fungal heat shock protein 60","volume":"54","author":"Raggam","year":"2010","journal-title":"Mycoses"},{"key":"2026041420234979200_cit41","doi-asserted-by":"crossref","first-page":"1511","DOI":"10.1002\/(SICI)1097-0061(199812)14:16<1511::AID-YEA356>3.0.CO;2-S","article-title":"Oxidative stress responses of the yeast Saccharomyces cerevisiae","volume":"14","author":"Jamieson","year":"1998","journal-title":"Yeast"},{"key":"2026041420234979200_cit42","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1016\/j.freeradbiomed.2009.11.010","article-title":"Yeast frataxin mutants display decreased superoxide dismutase activity crucial to promote protein oxidative damage","volume":"48","author":"Irazusta","year":"2010","journal-title":"Free Radical Biol. Med."},{"key":"2026041420234979200_cit43","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1038\/nrmicro2519","article-title":"Applied and industrial microbiology untapped potential: exploiting fungi in bioremediation of hazardous chemicals","volume":"9","author":"Harms","year":"2011","journal-title":"Nat. Rev. Microbiol."},{"key":"2026041420234979200_cit44","doi-asserted-by":"crossref","first-page":"1043","DOI":"10.1039\/c3mt00107e","article-title":"Mechanisms other than activation of the iron regulon account for the hyper-resistance to cobalt of a Saccharomyces cerevisae strain obtained by evolutionary engineering","volume":"5","author":"Alkim","year":"2013","journal-title":"Metallomics"}],"container-title":["Metallomics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/metallomics\/article-pdf\/11\/9\/1558\/41774421\/c9mt00137a.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/metallomics\/article-pdf\/11\/9\/1558\/41774421\/c9mt00137a.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T00:46:49Z","timestamp":1776214009000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/metallomics\/article\/11\/9\/1558\/5961725"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,9]]},"references-count":44,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2019,9,18]]}},"URL":"https:\/\/doi.org\/10.1039\/c9mt00137a","relation":{},"ISSN":["1756-5901","1756-591X"],"issn-type":[{"value":"1756-5901","type":"print"},{"value":"1756-591X","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2019,9]]},"published":{"date-parts":[[2019,9]]},"assertion":[{"value":"This document is Similarity Check deposited","name":"similarity_check","explanation":{"URL":"https:\/\/www.crossref.org\/services\/similarity-check\/"},"group":{"name":"similarity_check","label":"SIMILARITY CHECK"}},{"value":"Supplementary Information","URL":"https:\/\/doi.org\/10.1039\/C9MT00137A","name":"related_data","group":{"name":"related_data","label":"RELATED DATA"}},{"value":"Meriellen Dias (ORCID)","URL":"http:\/\/orcid.org\/0000-0002-2600-4582","name":"identifier","group":{"name":"author_identifiers","label":"IDENTIFIERS"}},{"value":"Silas Perdig\u00e3o Cota de Almeida (ORCID)","URL":"http:\/\/orcid.org\/0000-0002-5038-4597","name":"identifier","group":{"name":"author_identifiers","label":"IDENTIFIERS"}},{"value":"Lidiane Maria de Andrade (ORCID)","URL":"http:\/\/orcid.org\/0000-0003-4356-7077","name":"identifier","group":{"name":"author_identifiers","label":"IDENTIFIERS"}},{"value":"Enrique Eduardo Rozas (ORCID)","URL":"http:\/\/orcid.org\/0000-0003-4840-8315","name":"identifier","group":{"name":"author_identifiers","label":"IDENTIFIERS"}},{"value":"Maria Anita Mendes (ORCID)","URL":"http:\/\/orcid.org\/0000-0002-2078-9286","name":"identifier","group":{"name":"author_identifiers","label":"IDENTIFIERS"}},{"value":"Single-blind","order":2,"name":"peer_review_method","group":{"name":"peer_review_method","label":"PEER REVIEW METHOD"}},{"value":"Received 24 May 2019; Accepted 29 August 2019;  Advance Article published 4 September 2019;  Version of Record published 18 September 2019","order":1,"name":"history","group":{"name":"publication_history","label":"PUBLICATION HISTORY"}}]}}