{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T09:19:19Z","timestamp":1791278359424,"version":"4.1.0"},"reference-count":93,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2020,4,7]],"date-time":"2020-04-07T00:00:00Z","timestamp":1586217600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"},{"start":{"date-parts":[[2021,4,7]],"date-time":"2021-04-07T00:00:00Z","timestamp":1617753600000},"content-version":"am","delay-in-days":365,"URL":"http:\/\/rsc.li\/journals-terms-of-use"}],"funder":[{"DOI":"10.13039\/501100000972","name":"Australian Agency for International Development","doi-asserted-by":"publisher","award":["International Mining for Development Centre"],"award-info":[{"award-number":["International Mining for Development Centre"]}],"id":[{"id":"10.13039\/501100000972","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR-10-LABX-21, LABEX RESSOURCES21"],"award-info":[{"award-number":["ANR-10-LABX-21, LABEX RESSOURCES21"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000923","name":"Australian Research Council","doi-asserted-by":"publisher","award":["Discovery Early Career Researcher Award (DE1601004"],"award-info":[{"award-number":["Discovery Early Career Researcher Award (DE1601004"]}],"id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000923","name":"Australian Research Council","doi-asserted-by":"publisher","award":["Discovery Project (DP140100176)"],"award-info":[{"award-number":["Discovery Project (DP140100176)"]}],"id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000972","name":"Australian Agency for International Development","doi-asserted-by":"crossref","award":["International Mining for Development Centre"],"award-info":[{"award-number":["International Mining for Development Centre"]}],"id":[{"id":"10.13039\/501100000972","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"crossref","award":["ANR-10-LABX-21, LABEX RESSOURCES21"],"award-info":[{"award-number":["ANR-10-LABX-21, LABEX RESSOURCES21"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000923","name":"Australian Research Council","doi-asserted-by":"crossref","award":["Discovery Early Career Researcher Award (DE1601004"],"award-info":[{"award-number":["Discovery Early Career Researcher Award (DE1601004"]}],"id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000923","name":"Australian Research Council","doi-asserted-by":"crossref","award":["Discovery Project (DP140100176)"],"award-info":[{"award-number":["Discovery Project (DP140100176)"]}],"id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["rsc.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2020,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Three metallophyte species, Persicaria capitata, P. puncata (Polygonaceae), Conyza cordata (Asteraceae) from mineral wastes in the Zambian copper\u2013cobalt belt were studied. This study focused on the elemental distribution in the roots, stems and leaves, using a range of techniques: micro-PIXE, SEM-EDS synchrotron XFM and XAS. The species differed in their responses to growing on Co\u2013Cu-enriched soils: Persicaria puncata is a Co hyperaccumulator (up to 1060 \u03bcg g\u22121 in leaves), while Persicaria capitata and Conyza cordata are Co-excluders. All three species are Cu-accumulators. The highest concentrations of Cu\u2013Co are in the epidermal cells, whereas in Persicaria puncata Co was also enriched in the phloem. The Co coordination chemistry shows that an aqueous Co(ii)\u2013tartrate complex was the predominant component identified in all plants and tissues, along with a minor component of a Co(iii) compound with oxygen donor ligands. For Cu, there was considerable variation in the Cu speciation in the various tissues and across the three species. In contrast to hyperaccumulator plants, excluder and accumulator type plants have received far less attention. This study highlights the different biopathways of transition elements (Cu, Co) in hyper-tolerant plant species showing different responses to metalliferous environments.<\/jats:p>","DOI":"10.1039\/c9mt00263d","type":"journal-article","created":{"date-parts":[[2020,4,7]],"date-time":"2020-04-07T15:16:00Z","timestamp":1586272560000},"page":"682-701","update-policy":"https:\/\/doi.org\/10.1039\/rsc_crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Elemental distribution and chemical speciation of copper and cobalt in three metallophytes from the copper\u2013cobalt belt in Northern Zambia"],"prefix":"10.1093","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0922-5065","authenticated-orcid":false,"given":"Antony","family":"van der Ent","sequence":"first","affiliation":[{"name":"Centre for Mined Land Rehabilitation, Sustainable Minerals Institute, The University of Queensland, Queensland, Australia"},{"name":"Universit\u00e9 de Lorraine \u2013 INRA, Laboratoire Sols et Environnement, UMR 1120, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Royd","family":"Vinya","sequence":"additional","affiliation":[{"name":"School of Natural Resources, Plant and Environmental Sciences Department, The Copperbelt University, Zambia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8298-2866","authenticated-orcid":false,"given":"Peter D","family":"Erskine","sequence":"additional","affiliation":[{"name":"Centre for Mined Land Rehabilitation, Sustainable Minerals Institute, The University of Queensland, Queensland, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7ois","family":"Malaisse","sequence":"additional","affiliation":[{"name":"Botanic Garden Meise, Belgium"},{"name":"Biodiversity and Landscape Unit, Gembloux Agro-Bio Tech, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1016-1206","authenticated-orcid":false,"given":"Wojciech J","family":"Przyby\u0142owicz","sequence":"additional","affiliation":[{"name":"AGH University of Science and Technology, Faculty of Physics & Applied Computer Science, 30-059 Krak\u00f3w, Poland"},{"name":"Department of Botany and Zoology, Stellenbosch University, Private Bag X1, Matieland 7602, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alban D","family":"Barnabas","sequence":"additional","affiliation":[{"name":"Materials Research Department, iThemba LABS National Research Foundation, PO Box 722, Somerset West 7129, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3472-8628","authenticated-orcid":false,"given":"Hugh H","family":"Harris","sequence":"additional","affiliation":[{"name":"Department of Chemistry, The University of Adelaide, South Australia, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2652-8674","authenticated-orcid":false,"given":"Jolanta","family":"Mesjasz-Przyby\u0142owicz","sequence":"additional","affiliation":[{"name":"Department of Botany and Zoology, Stellenbosch University, Private Bag X1, Matieland 7602, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,4,7]]},"reference":[{"key":"2021121004465770600_cit1","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1111\/nph.14175","article-title":"Copper and cobalt accumulation in plants: a critical assessment of the current state of knowledge","volume":"213","author":"Lange","year":"2016","journal-title":"New Phytol."},{"key":"2021121004465770600_cit2","article-title":"Heavy Metal Vegetation of the World (in German)","volume-title":"Geobotanica Selecta","author":"Ernst","year":"1974"},{"key":"2021121004465770600_cit3","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1017\/CBO9780511805561.003","article-title":"Metallophytes: the unique biological resource, its ecology and conservational status in Europe, central Africa and Latin America","volume-title":"Ecology of industrial pollution","author":"Baker","year":"2010"},{"key":"2021121004465770600_cit4","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1080\/01904168109362867","article-title":"Accumulation and excluders \u2013 strategies in the response of plants to heavy metals","volume":"3","author":"Baker","year":"1981","journal-title":"J. Plant Nutr."},{"key":"2021121004465770600_cit5","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1111\/j.1469-8137.1987.tb04685.x","article-title":"Metal tolerance","volume":"106","author":"Baker","year":"1987","journal-title":"New Phytol."},{"key":"2021121004465770600_cit6","first-page":"81","article-title":"Terrestrial higher plants which hyper accumulate metallic elements","volume":"1","author":"Baker","year":"1989","journal-title":"Biorecovery"},{"issue":"11","key":"2021121004465770600_cit7","doi-asserted-by":"crossref","first-page":"1707","DOI":"10.1016\/j.biochi.2006.07.003","article-title":"Toxic metal accumulation, responses to exposure and mechanisms of tolerance in plants","volume":"88","author":"Clemens","year":"2006","journal-title":"Biochimie"},{"key":"2021121004465770600_cit8","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1007\/BF02140724","article-title":"Copper and cobalt uptake by metallophytes from Zaire","volume":"53","author":"Morrison","year":"1979","journal-title":"Plant Soil"},{"key":"2021121004465770600_cit9","volume-title":"The heavy metal tolerant flora of Southcentral Africa: a multidisciplinary approach","author":"Brooks","year":"1985"},{"key":"2021121004465770600_cit10","first-page":"166","article-title":"Hyperaccumulation of Copper and Cobalt \u2013 A Review","volume":"113","author":"Brooks","year":"1980","journal-title":"Bull. Soc. R. Bot. Belg."},{"key":"2021121004465770600_cit11","first-page":"179","article-title":"The elemental content of metallophytes from the copper\/cobalt deposits of Central Africa","volume":"119","author":"Brooks","year":"1986","journal-title":"Bull. Soc. R. Bot. Belg."},{"key":"2021121004465770600_cit12","doi-asserted-by":"crossref","first-page":"5","DOI":"10.5091\/plecevo.2010.411","article-title":"Copper endemism in the Congolese flora: a database of copper affinity and conservational value of cuprophytes","volume":"143","author":"Faucon","year":"2010","journal-title":"Plant Ecol. Evol."},{"key":"2021121004465770600_cit13","doi-asserted-by":"crossref","first-page":"472","DOI":"10.2307\/3544336","article-title":"Copper and Cobalt in Vegetation of Fungurume, Shaba Province, Zai\u00a8re","volume":"33","author":"Malaisse","year":"1979","journal-title":"Oikos"},{"key":"2021121004465770600_cit14","volume-title":"Copper\/cobalt Flora of Upper Katanga Copperbelt","author":"Malaisse","year":"2006"},{"key":"2021121004465770600_cit15","first-page":"214","article-title":"Functions and homeostasis of zinc, copper, and nickel in plants","volume-title":"Molecular biology of metal homeostasis and detoxification from microbes to man","author":"Kr\u00e4mer","year":"2006"},{"key":"2021121004465770600_cit16","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1071\/FP08288","article-title":"Copper in plants: acquisition, transport and interactions","volume":"36","author":"Yruela","year":"2009","journal-title":"Funct. Plant Biol."},{"key":"2021121004465770600_cit17","doi-asserted-by":"crossref","first-page":"374","DOI":"10.3389\/fpls.2013.00374","article-title":"Compartmentation and complexation of metals in hyperaccumulator plants","volume":"4","author":"Leitenmaier","year":"2013","journal-title":"Front. Plant Sci."},{"key":"2021121004465770600_cit18","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1007\/s11104-007-9463-6","article-title":"An X-ray absorption spectroscopy investigation of speciation and biotransformation of copper in Elsholtzia splendens","volume":"302","author":"Shi","year":"2007","journal-title":"Plant Soil"},{"key":"2021121004465770600_cit19","first-page":"1","volume-title":"Microspectroscopic study of cobalt speciation and localization in hyperaccumulator Alyssum murale","author":"Tappero","year":"2009"},{"key":"2021121004465770600_cit20","volume-title":"Cycles of soil-carbon, nitrogen, phosphorus, sulfur, micronutrients","author":"Stevenson","year":"1986"},{"issue":"Special Issue 2","key":"2021121004465770600_cit21","first-page":"31","article-title":"Toxicities and Tolerance of Mineral Elements. Boron, Cobalt, Molybdenum and Nickel in Crop Plants","volume":"4","author":"Singh","year":"2010","journal-title":"Plant Stress"},{"key":"2021121004465770600_cit22","first-page":"287","volume-title":"Aspects of the accumulation of cobalt, copper and nickel by plants","author":"Morrison","year":"1980"},{"key":"2021121004465770600_cit23","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1007\/s11104-009-0105-z","article-title":"Copper tolerance in the cuprophyte Haumaniastrum katangense (SMoore) P. A. Duvign & Plancke","volume":"328","author":"Chipeng","year":"2009","journal-title":"Plant Soil"},{"key":"2021121004465770600_cit24","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.envexpbot.2011.12.015","article-title":"Differences in copper accumulation and copper stress between eight populations of Haumaniastrum katangense","volume":"79","author":"Peng","year":"2012","journal-title":"Environ. Exp. Bot."},{"key":"2021121004465770600_cit25","first-page":"177","article-title":"Copper tolerance in some Californian populations of the monkey flower, Mimulus guttatus","volume":"177","author":"Allen","year":"1971","journal-title":"Proc. R. Soc. B"},{"key":"2021121004465770600_cit26","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1038\/hdy.1983.30","article-title":"The genetic control of copper tolerance in the yellow monkey flower, Mimulus guttatus","volume":"50","author":"Macnair","year":"1983","journal-title":"Heredity"},{"key":"2021121004465770600_cit27","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1007\/s11104-008-9801-3","article-title":"Soil influence on Cu and Co uptake and plant size in the cuprophytes Crepidorhopalon perennis and C. tenuis (Scrophulariaceae) in SC Africa","volume":"317","author":"Faucon","year":"2008","journal-title":"Plant Soil"},{"key":"2021121004465770600_cit28","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.envexpbot.2012.04.012","article-title":"Copper tolerance and accumulation in two cuprophytes of South Central Africa: Crepidorhopalon perennis and C. tenuis (Linderniaceae)","volume":"84","author":"Faucon","year":"2012","journal-title":"Environ. Exp. Bot."},{"key":"2021121004465770600_cit29","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.envexpbot.2018.04.009","article-title":"Variation in copper and cobalt tolerance and accumulation among six populations of the facultative metallophyte Anisopappus chinensis (Asteraceae)","volume":"153","author":"Lange","year":"2018","journal-title":"Environ. Exp. Bot."},{"key":"2021121004465770600_cit30","doi-asserted-by":"crossref","first-page":"1179","DOI":"10.1016\/j.chemosphere.2004.01.026","article-title":"Growth response and phytoextraction of copper at different levels in soils by Elsholtzia splendens","volume":"55","author":"Jiang","year":"2004","journal-title":"Chemosphere"},{"key":"2021121004465770600_cit31","doi-asserted-by":"crossref","first-page":"880","DOI":"10.1016\/j.envint.2005.05.032","article-title":"Copper uptake by four Elsholtzia ecotypes supplied with varying levels of copper in solution culture","volume":"31","author":"Weng","year":"2005","journal-title":"Environ. Int."},{"key":"2021121004465770600_cit32","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1007\/BF02184314","article-title":"Studies on copper and cobalt tolerance in three closely related taxa within the genus Silene L (Caryophyllaceae) from Za\u0131\u00a8re","volume":"73","author":"Baker","year":"1983","journal-title":"Plant Soil"},{"key":"2021121004465770600_cit33","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1080\/15226510008500041","article-title":"Phytoextraction of Soil Cobalt Using Hyperaccumulator Plants","volume":"2","author":"Malik","year":"2000","journal-title":"Int. J. Phytorem."},{"key":"2021121004465770600_cit34","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1656\/045.016.0511","article-title":"Uptake and Accumulation of Cobalt by Alyssum bracteatum, an Endemic Iranian Ni Hyperaccumulator","volume":"16","author":"Ghaderian","year":"2009","journal-title":"Northeast Nat."},{"key":"2021121004465770600_cit35","doi-asserted-by":"crossref","first-page":"21378","DOI":"10.3390\/ijms160921378","article-title":"Can Clethra barbinervis distinguish nickel and cobalt in uptake and translocation?","volume":"16","author":"Yamaguchi","year":"2015","journal-title":"Int. J. Mol. Sci."},{"key":"2021121004465770600_cit36","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1080\/713779223","article-title":"Nickel and cobalt phytoextraction by the hyperaccumulator Berkheya coddii: implications for polymetallic phytomining and phytoremediation","volume":"5","author":"Keeling","year":"2003","journal-title":"Int. J. Phytorem."},{"key":"2021121004465770600_cit37","volume-title":"The biological chemistry of the elements: the inorganic chemistry of life","author":"Frausto da Silva","year":"1991"},{"key":"2021121004465770600_cit38","doi-asserted-by":"crossref","first-page":"1081","DOI":"10.1111\/j.1469-8137.1969.tb06509.x","article-title":"The uptake and accumulation of copper by Becium homblei (De Wild.) Duvig & Plancke","volume":"68","author":"Reilly","year":"1969","journal-title":"New Phytol."},{"key":"2021121004465770600_cit39","doi-asserted-by":"crossref","first-page":"993","DOI":"10.1111\/j.1469-8137.1970.tb02478.x","article-title":"The accumulation and binding of copper in leaf tissue of Becium homblei (De Wild.) Duvign & Plancke","volume":"69","author":"Reilly","year":"1970","journal-title":"New Phytol."},{"key":"2021121004465770600_cit40","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1016\/S0031-9422(00)84165-5","article-title":"The diverse chemical forms of heavy metals in tissue extracts of some metallophytes from Shaba province, Zaire","volume":"20","author":"Morrison","year":"1981","journal-title":"Phytochemistry"},{"key":"2021121004465770600_cit41","doi-asserted-by":"crossref","first-page":"2257","DOI":"10.1016\/j.chemosphere.2006.12.006","article-title":"Accumulation, speciation and cellular localization of copper in Sesbania drummondii","volume":"67","author":"Sahi","year":"2007","journal-title":"Chemosphere"},{"key":"2021121004465770600_cit42","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1016\/j.nimb.2015.09.005","article-title":"Quantitative mapping of elemental distribution in leaves of the metallophytes Helichrysum candolleanum, Blepharis aspera, and Blepharis diversispina from Selkirk Cu-Ni mine, Botswana","volume":"363","author":"Koosaletse-Mswela","year":"2015","journal-title":"Nucl. Instrum. Methods Phys. Res., Sect. B"},{"key":"2021121004465770600_cit43","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1111\/nph.14810","article-title":"X-ray elemental mapping techniques for elucidating the ecophysiology of hyperaccumulator plants","volume":"218","author":"van der Ent","year":"2018","journal-title":"New Phytol."},{"key":"2021121004465770600_cit44","first-page":"585","article-title":"Disponibilite\u00b4 du nickel le long d\u2019une topose\u00b4quence de sols de\u00b4veloppe\u00b4s sur roches ultramafiques de Nouvelle-Cale\u00b4donie","volume":"321","author":"Becquer","year":"1995","journal-title":"C. R. Seances Acad. Sci. Ser. 2"},{"key":"2021121004465770600_cit45","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/S0022-5320(69)90033-1","article-title":"A low-viscosity epoxy resin embedding medium for electron microscopy","volume":"26","author":"Spurr","year":"1969","journal-title":"J. Ultrastruct. Res."},{"key":"2021121004465770600_cit46","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1063\/1.3625343","article-title":"The X-ray fluorescence microscopy beamline at the Australian synchrotron","volume":"1365","author":"Paterson","year":"2011","journal-title":"AIP Conf. Proc."},{"key":"2021121004465770600_cit47","first-page":"1234","article-title":"The Maia Spectroscopy Detector System: Engineering for Integrated Pulse Capture, Low-Latency Scanning and Real-Time Processing","volume-title":"AIP Conf. Proc.","author":"Kirkham","year":"2010"},{"key":"2021121004465770600_cit48","first-page":"012001","article-title":"Maia X-ray Microprobe Detector Array System","volume":"499","author":"Siddons","year":"2014","journal-title":"J. Phys.: Conf. Ser."},{"key":"2021121004465770600_cit49","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.nima.2009.11.035","article-title":"Elemental X-ray imaging using the Maia detector array: The benefits and challenges of large solid-angle","volume":"619","author":"Ryan","year":"2010","journal-title":"Nucl. Instrum. Methods Phys. Res., Sect. A"},{"key":"2021121004465770600_cit50","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/0168-583X(95)00581-1","volume":"104","author":"Prozesky","year":"1995","journal-title":"Nucl. Instrum. Methods Phys. Res., Sect. B"},{"key":"2021121004465770600_cit51","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1002\/(SICI)1097-4539(199907\/08)28:4<237::AID-XRS342>3.0.CO;2-8","article-title":"Biological applications of the NAC nuclear microprobe","volume":"28","author":"Przybylowicz","year":"1999","journal-title":"X-Ray Spectrom."},{"key":"2021121004465770600_cit52","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1111\/nph.12276","article-title":"Copper speciation and isotopic fractionation in plants: uptake and translocation mechanisms","volume":"199","author":"Ryan","year":"2013","journal-title":"New Phytol."},{"key":"2021121004465770600_cit53","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/0168-583X(90)90259-W","article-title":"Quantitative analysis of PIXE spectra in geoscience applications","volume":"49","author":"Ryan","year":"1990","journal-title":"Nucl. Instrum. Methods Phys. Res., Sect. B"},{"key":"2021121004465770600_cit54","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1002\/ima.1007","article-title":"Quantitative trace element imaging using PIXE and the nuclear microprobe","volume":"11","author":"Ryan","year":"2000","journal-title":"Int. J. Imag. Syst. Technol."},{"key":"2021121004465770600_cit55","doi-asserted-by":"crossref","first-page":"586","DOI":"10.1021\/ac60259a007","article-title":"Limits for qualitative detection and quantitative determination: Application to radiochemistry","volume":"40","author":"Currie","year":"1968","journal-title":"Anal. Chem."},{"key":"2021121004465770600_cit56","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/0168-583X(93)95545-G","article-title":"Dynamic analysis: on-line quantitative PIXE microanalysis and its use in overlap- resolved elemental mapping","volume":"77","author":"Ryan","year":"1993","journal-title":"Nucl. Instrum. Methods Phys. Res., Sect. B"},{"key":"2021121004465770600_cit57","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/0168-583X(95)00404-1","article-title":"A new method for on-line true-elemental imaging using PIXE and the proton microprobe","volume":"104","author":"Ryan","year":"1995","journal-title":"Nucl. Instrum. Methods Phys. Res., Sect. B"},{"key":"2021121004465770600_cit58","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/j.nimb.2005.01.054","article-title":"Nuclear Microprobe \u2013 Synchrotron Synergy: Towards Integrated Quantitative Real-time Elemental Imaging using PIXE and SXRF","volume":"231","author":"Ryan","year":"2005","journal-title":"Nucl. Instrum. Methods Phys. Res., Sect. B"},{"key":"2021121004465770600_cit59","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/0168-583X(86)90291-0","article-title":"A semiautomatic algorithm for Rutherford backscattering analysis","volume":"15","author":"Doolittle","year":"1986","journal-title":"Nucl. Instrum. Methods Phys. Res., Sect. B"},{"key":"2021121004465770600_cit60","doi-asserted-by":"crossref","first-page":"9683","DOI":"10.1038\/s41598-018-26891-7","article-title":"Simultaneous hyperaccumulation of nickel and cobalt in Glochidion cf. sericeum (Phyllanthaceae): elemental distribution and speciation","volume":"8","author":"van der Ent","year":"2018","journal-title":"Sci. Rep."},{"key":"2021121004465770600_cit61","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1111\/j.1558-5646.1977.tb01008.x","article-title":"The evolutionary effects of metalliferous and other anomalous soils in south central Africa","volume":"31","author":"Wild","year":"1977","journal-title":"Evolution"},{"key":"2021121004465770600_cit62","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1016\/0169-5347(87)90135-2","article-title":"Heavy metal tolerance in plants: a model evolutionary system","volume":"2","author":"Macnair","year":"1987","journal-title":"Trends Ecol. Evol."},{"key":"2021121004465770600_cit63","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1080\/0735-260291044359","article-title":"The genetic basis of metal hyperaccumulation in plants","volume":"21","author":"Pollard","year":"2002","journal-title":"Crit. Rev. Plant Sci."},{"key":"2021121004465770600_cit64","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1126\/science.199.4331.887","article-title":"Aeolanthus biformifolius De Wild.: A Hyperaccumulator of Copper from Zaire","volume":"199","author":"Malaisse","year":"1978","journal-title":"Science"},{"key":"2021121004465770600_cit65","first-page":"193","article-title":"Metal accumulating plants","volume-title":"Phytoremediation of Toxic Metals: Using Plants to Clean Up the Environment","author":"Reeves","year":"2000"},{"key":"2021121004465770600_cit66","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1007\/1-4020-4688-X_2","article-title":"Hyperaccumulation of trace elements by plants","volume-title":"Phytoremediation of metal-contaminated soils","author":"Reeves","year":"2006"},{"key":"2021121004465770600_cit67","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1016\/0168-583X(94)95174-8","article-title":"Proton microprobe and X-ray fluorescence investigations of nickel distribution in serpentine flora from South Africa","volume":"89","author":"Mesjasz-Przyby\u0142owicz","year":"1994","journal-title":"Nucl. Instrum. Methods Phys. Res., Sect. B"},{"key":"2021121004465770600_cit68","first-page":"593","article-title":"Elemental distribution in Senecio anomalochrous, a Ni hyperaccumulator from South Africa","volume":"97","author":"Mesjasz-Przybylowicz","year":"2001","journal-title":"S. Afr. J. Sci."},{"key":"2021121004465770600_cit69","doi-asserted-by":"crossref","first-page":"2291","DOI":"10.1093\/jexbot\/52.365.2291","article-title":"Cellular compartmentation of nickel in the hyperaccumulators Alyssum lesbiacum, Alyssum bertolonii and Thlaspi goesingense","volume":"52","author":"K\u00fcpper","year":"2001","journal-title":"J. Exp. Bot."},{"key":"2021121004465770600_cit70","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1111\/j.0016-8025.2003.01170.x","article-title":"Sub-cellular localization of Ni in the hyperaccumulator, Hybanthus floribundus (Lindley) F Muell","volume":"27","author":"Bidwell","year":"2004","journal-title":"Plant, Cell Environ."},{"key":"2021121004465770600_cit71","doi-asserted-by":"crossref","first-page":"1061","DOI":"10.1071\/FP03192","article-title":"Studies on spatial distribution of nickel in leaves and stems of the metal hyperaccumulator Stackhousia tryonii Bailey using nuclear microprobe (micro-PIXE) and EDXS techniques","volume":"31","author":"Bhatia","year":"2004","journal-title":"Funct. Plant Biol."},{"key":"2021121004465770600_cit72","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.nimb.2007.02.017","article-title":"In-vacuum micro-PIXE analysis of biological specimens in frozen-hydrated state","volume":"260","author":"Tylko","year":"2007","journal-title":"Nucl. Instrum. MethodsPhys. Res., Sect. B"},{"key":"2021121004465770600_cit73","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1016\/j.nimb.2007.11.053","article-title":"Quantitative elemental localisation in leaves and stems of nickel hyperaccumulating shrub Hybanthus floribundus subsp. floribundus using micro-PIXE spectroscopy","volume":"266","author":"Kachenko","year":"2008","journal-title":"Nucl. Instrum. Methods Phys. Res., Sect. B"},{"key":"2021121004465770600_cit74","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1016\/S0168-583X(97)00228-0","article-title":"Quantitative micro-PIXE comparison of elemental distribution in Ni-hyperaccumulating and nonaccumulating genotypes of Senecio coronatus","volume":"130","author":"Mesjasz-Przyby\u0142owicz","year":"1997","journal-title":"Nucl. Instrum. Methods Phys. Res., Sect. B"},{"key":"2021121004465770600_cit75","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1007\/s11104-005-0974-8","article-title":"Nickel localization and response to increasing Ni soil levels in leaves of the Ni hyperaccumulator Alyssum murale","volume":"265","author":"Broadhurst","year":"2004","journal-title":"Plant Soil"},{"key":"2021121004465770600_cit76","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1002\/xrs.1304","article-title":"PIXE and metal hyperaccumulation: from soil to plants and insects","volume":"40","author":"Mesjasz-Przyby\u0142owicz","year":"2011","journal-title":"X-Ray Spectrom."},{"key":"2021121004465770600_cit77","doi-asserted-by":"crossref","first-page":"1513","DOI":"10.1111\/nph.13712","article-title":"Extreme nickel hyperaccumulation in the vascular tracts of the tree Phyllanthus balgooyi from Borneo","volume":"209","author":"Mesjasz-Przybylowicz","year":"2016","journal-title":"New Phytol."},{"key":"2021121004465770600_cit78","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1007\/s11104-016-2825-1","article-title":"Nickel translocation via the phloem in the hyperaccumulator Noccaea caerulescens (Brassicaceae)","volume":"404","author":"Deng","year":"2016","journal-title":"Plant Soil"},{"key":"2021121004465770600_cit79","doi-asserted-by":"crossref","first-page":"41861","DOI":"10.1038\/srep41861","article-title":"Nickel biopathways in tropical nickel hyperaccumulating trees from Sabah (Malaysia)","volume":"7","author":"van der Ent","year":"2017","journal-title":"Sci. Rep."},{"key":"2021121004465770600_cit80","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1007\/BF02187261","article-title":"Copper and cobalt uptake by Haumaniastrum species","volume":"48","author":"Brooks","year":"1977","journal-title":"Plant Soil"},{"key":"2021121004465770600_cit81","first-page":"253","article-title":"Hyperaccumulation of nickel by serpentine plants","volume-title":"The Vegetation of Ultramafic (Serpentine) Soils","author":"Reeves","year":"1992"},{"key":"2021121004465770600_cit82","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1007\/s11104-012-1287-3","article-title":"Hyperaccumulators of metal and metalloid trace elements: Facts and fiction","volume":"362","author":"van der, Ent","year":"2013","journal-title":"Plant Soil"},{"key":"2021121004465770600_cit83","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1146\/annurev-arplant-042809-112156","article-title":"Metal hyperaccumulation in plants","volume":"61","author":"Kr\u00e4mer","year":"2010","journal-title":"Annu. Rev. Plant Biol."},{"key":"2021121004465770600_cit84","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1007\/BF02107201","article-title":"Copper and cobalt in African species of Aeolanthus Mart (Plectranthinae, Labiatae)","volume":"50","author":"Brooks","year":"1978","journal-title":"Plant Soil"},{"key":"2021121004465770600_cit85","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1111\/j.1469-8137.1981.tb01749.x","article-title":"The uptake of copper by plants of Mimulus guttatus differing in genotype primarily at a single major copper tolerance locus","volume":"88","author":"Macnair","year":"1981","journal-title":"New Phytol."},{"key":"2021121004465770600_cit86","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1038\/hdy.1993.23","article-title":"general model for the genetic control of copper tolerance in Silene vulgaris: evidence from crosses between plants from different tolerant populations","volume":"70","author":"Schat","year":"1993","journal-title":"Heredity"},{"key":"2021121004465770600_cit87","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/j.envpol.2003.09.019","article-title":"Copper uptake by Elsholtzia splendens and Silene vulgaris and assessment of copper phytoavailability in contaminated soils","volume":"128","author":"Song","year":"2004","journal-title":"Environ. Pollut."},{"key":"2021121004465770600_cit88","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1038\/215667a0","article-title":"Accumulation of Copper by Some Zambian Plants","volume":"215","author":"Reilly","year":"1967","journal-title":"Nature"},{"key":"2021121004465770600_cit89","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/0375-6742(95)00048-8","article-title":"A re-evaluation of Haumaniastrum species as geobotanical indicators of copper and cobalt","volume":"56","author":"Paton","year":"1996","journal-title":"J. Geochem. Explor."},{"key":"2021121004465770600_cit90","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1007\/s11104-007-9405-3","article-title":"Revisiting copper and cobalt concentrations in supposed hyperaccumulators from SC Africa: influence of washing and metal concentrations in soil","volume":"301","author":"Faucon","year":"2007","journal-title":"Plant Soil"},{"key":"2021121004465770600_cit91","doi-asserted-by":"crossref","first-page":"586","DOI":"10.1039\/C8MT00300A","article-title":"Abnormal concentrations of Cu-Co in Haumaniastrum katangense, Haumaniastrum robertii and Aeolanthus biformifolius: contamination or hyperaccumulation?","volume":"11","author":"van der Ent","year":"2019","journal-title":"Metallomics"},{"key":"2021121004465770600_cit92","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1111\/j.1061-2971.2004.00367.x","article-title":"Research Priorities for Conservation of Metallophyte Biodiversity and their Potential for Restoration and Site Remediation","volume":"12","author":"Whiting","year":"2004","journal-title":"Restor. Ecol."},{"key":"2021121004465770600_cit93","article-title":"The potential of Zambian copper-cobalt metallophytes for phytoremediation of minerals wastes","volume-title":"Africa\u2019s Mineral Fortune. The Science and Politics of Mining and Sustainable Development","author":"van der Ent","year":"2018"}],"container-title":["Metallomics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/metallomics\/article-pdf\/12\/5\/682\/41703055\/c9mt00263d.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/metallomics\/article-pdf\/12\/5\/682\/41703055\/c9mt00263d.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,12,10]],"date-time":"2021-12-10T05:11:10Z","timestamp":1639113070000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/metallomics\/article\/12\/5\/682\/5961810"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,4,7]]},"references-count":93,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2020,5,1]]}},"URL":"https:\/\/doi.org\/10.1039\/c9mt00263d","relation":{},"ISSN":["1756-5901","1756-591X"],"issn-type":[{"value":"1756-5901","type":"print"},{"value":"1756-591X","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2020,5]]},"published":{"date-parts":[[2020,4,7]]},"assertion":[{"value":"This document is Similarity Check deposited","name":"similarity_check","explanation":{"URL":"https:\/\/www.crossref.org\/services\/similarity-check\/"},"group":{"name":"similarity_check","label":"SIMILARITY CHECK"}},{"value":"Supplementary Information","URL":"https:\/\/doi.org\/10.1039\/C9MT00263D","name":"related_data","group":{"name":"related_data","label":"RELATED DATA"}},{"value":"Antony van der Ent (ORCID)","URL":"http:\/\/orcid.org\/0000-0003-0922-5065","name":"identifier","group":{"name":"author_identifiers","label":"IDENTIFIERS"}},{"value":"Antony van der Ent (ResearcherID)","URL":"http:\/\/www.researcherid.com\/rid\/A-7486-2015","name":"identifier","group":{"name":"author_identifiers","label":"IDENTIFIERS"}},{"value":"Royd Vinya (ResearcherID)","URL":"http:\/\/www.researcherid.com\/rid\/G-5413-2011","name":"identifier","group":{"name":"author_identifiers","label":"IDENTIFIERS"}},{"value":"Peter D. Erskine (ORCID)","URL":"http:\/\/orcid.org\/0000-0002-8298-2866","name":"identifier","group":{"name":"author_identifiers","label":"IDENTIFIERS"}},{"value":"Peter D. Erskine (ResearcherID)","URL":"http:\/\/www.researcherid.com\/rid\/U-3136-2018","name":"identifier","group":{"name":"author_identifiers","label":"IDENTIFIERS"}},{"value":"Wojciech J. Przyby\u0142owicz (ORCID)","URL":"http:\/\/orcid.org\/0000-0003-1016-1206","name":"identifier","group":{"name":"author_identifiers","label":"IDENTIFIERS"}},{"value":"Hugh H. Harris (ORCID)","URL":"http:\/\/orcid.org\/0000-0002-3472-8628","name":"identifier","group":{"name":"author_identifiers","label":"IDENTIFIERS"}},{"value":"Jolanta Mesjasz-Przyby\u0142owicz (ORCID)","URL":"http:\/\/orcid.org\/0000-0003-2652-8674","name":"identifier","group":{"name":"author_identifiers","label":"IDENTIFIERS"}},{"value":"Single-blind","order":2,"name":"peer_review_method","group":{"name":"peer_review_method","label":"PEER REVIEW METHOD"}},{"value":"Received 30 October 2019; Accepted 16 March 2020;  Advance Article published 7 April 2020;  Version of Record published 27 May 2020","order":1,"name":"history","group":{"name":"publication_history","label":"PUBLICATION HISTORY"}}]}}