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A complete gene expression profile was therefore constructed to illustrate the anti-tumourigenesis effects of maslinic acid in Raji cells across five time-points. Microarray analysis was used to identify genes that were differentially expressed in maslinic acid treated Raji cells at 0, 4, 8, 12, 24 and 48 h. Extracted RNA was hybridized using the AffymetrixGeneChip to obtain expression profiles. A total of 109 genes were found to be significantly expressed over a period of 48 hours. By 12 hours, maslinic acid regulates the majority of genes involved in the cell cycle, p53 and NF-\u03baB signaling pathways. At the same time, XAF1, APAF1, SESN3, and TP53BP2 were evidently up-regulated, while oncogenes, FAIM, CD27, and RRM2B, were down-regulated by at least 2-fold. In conclusion, maslinic acid shows an hourly progression of gene expression in Raji cells.<\/jats:p>\n                  <jats:p\/>","DOI":"10.1039\/d0mo00168f","type":"journal-article","created":{"date-parts":[[2020,12,9]],"date-time":"2020-12-09T22:24:34Z","timestamp":1607552674000},"page":"252-259","update-policy":"https:\/\/doi.org\/10.1039\/rsc_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Temporal gene expression profiling of maslinic acid-treated Raji cells"],"prefix":"10.1093","volume":"17","author":[{"given":"Wai Meng","family":"Lau","sequence":"first","affiliation":[{"name":"Centre for Cancer Research, Faculty of Medicine and Health Sciences, Universiti Tunku Abdul Rahman, PT21144, Jalan Sungai Long a , Bandar Sungai Long, 43000 Kajang, Selangor, Malaysia \u00a0 ymlim@utar.edu.my \u00a0 +603 90191959 \u00a0 +603 90194722 etxn 178"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3388-9534","authenticated-orcid":false,"given":"Menaga","family":"Subramaniam","sequence":"additional","affiliation":[{"name":"Centre for Cancer Research, Faculty of Medicine and Health Sciences, Universiti Tunku Abdul Rahman, PT21144, Jalan Sungai Long a , Bandar Sungai Long, 43000 Kajang, Selangor, Malaysia \u00a0 ymlim@utar.edu.my \u00a0 +603 90191959 \u00a0 +603 90194722 etxn 178"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8508-9977","authenticated-orcid":false,"given":"Hoe Han","family":"Goh","sequence":"additional","affiliation":[{"name":"Institute of Systems Biology, Universiti Kebangsaan Malaysia, UKM Bangi b , Bangi, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1377-7655","authenticated-orcid":false,"given":"Yang Mooi","family":"Lim","sequence":"additional","affiliation":[{"name":"Department of Pre-Clinical Science, Faculty of Medicine and Health Sciences, Universiti Tunku Abdul Rahman c , Lot PT21144, Jalan Sungai Long, Bandar Sungai Long, 43000 Kajang, Selangor, Malaysia"},{"name":"Centre for Cancer Research, Faculty of Medicine and Health Sciences, Universiti Tunku Abdul Rahman, PT21144, Jalan Sungai Long a , Bandar Sungai Long, 43000 Kajang, Selangor, Malaysia \u00a0 ymlim@utar.edu.my \u00a0 +603 90191959 \u00a0 +603 90194722 etxn 178"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2020,12,8]]},"reference":[{"key":"2026010813083150800_cit1","doi-asserted-by":"crossref","first-page":"1267","DOI":"10.1002\/cbdv.200900193","article-title":"Chemopreventive properties of phytosterols and maslinic acid extracted from Coleus tuberosus in inhibiting the expression of EBV early-antigen in Raji cells","volume":"7","author":"Mooi","year":"2010","journal-title":"Chem. 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