{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T12:56:01Z","timestamp":1762520161305,"version":"3.37.3"},"reference-count":48,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2020,9,10]],"date-time":"2020-09-10T00:00:00Z","timestamp":1599696000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"},{"start":{"date-parts":[[2021,8,31]],"date-time":"2021-08-31T00:00:00Z","timestamp":1630368000000},"content-version":"am","delay-in-days":355,"URL":"http:\/\/rsc.li\/journals-terms-of-use"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31872437"],"award-info":[{"award-number":["31872437"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["31872437"],"award-info":[{"award-number":["31872437"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["rsc.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2020,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Dietary selenium (Se) deficiency can induce multifarious immune injury in tissues, accompanied by inflammation and a decreased expression of selenoproteins. The results of previous studies indicated that these issues are associated with Se-mediated microRNAs involved in immune regulation, although the specific mechanisms associated with these interactions have not been reported in the trachea of chickens. To explore the effects of Se deficiency in the trachea of chickens and the role of miR-196-5p, we established correlational models of tracheal injury in chickens. One hundred broilers were divided into four groups, including a control group (C group), a Se deficient group (L group), a lipopolysaccharide (LPS)-induced control group (C + LPS group) and a LPS-induced Se deficient group (L + LPS group). Light microscopy observations indicated that the infiltration of inflammatory cells was the major histopathological change caused by Se deficiency. Furthermore, ultrastructural observation of the tracheal epithelium and ciliary showed typical inflammatory signs owing to Se deficiency. We determined the targeting relationship between miR-196-5p and NF\u03baBIA by bioinformatics analysis. In the case of Se deficiency, the changes were detected as follows: 19 selenoproteins showed different degrees of decrease (p &amp;lt; 0.05). Significant inhibition of both antimicrobial peptides and immunoglobulin production were observed (p &amp;lt; 0.05). I\u03baB-\u03b1 (NF\u03baBIA) expression degraded with the increasing miR-196-5p (p &amp;lt; 0.05), and the NF-\u03baB pathway was activated. Thereafter, we can see a significant increase in the mRNA levels of inflammatory cytokines-related genes (tumor necrosis factor (TNF)-\u03b1, inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2, prostaglandin E (PTGE), interleukin (IL)-1\u03b2, IL-6) and protein expression of NF-\u03baB\/iNOS pathway-related genes (NF-\u03baB, iNOS, TNF-\u03b1, COX-2) (p &amp;lt; 0.05). The release of IL-2, interferon (IFN)-\u03b3 inhibited (p &amp;lt; 0.05) and the secretion of IL-4, IL-6 increased, suggesting the imbalance of Th1\/Th2 (Th, helper T cell) cytokines. Compared to the control, the mRNA and protein expression levels of the anti-inflammatory system components with antioxidant activity (PPAR-\u03b3\/HO-1) were in an inhibitory state (p &amp;lt; 0.05). Antioxidases (SOD, CAT, GSH-Px) activities were suppressed. The activities of the peroxide markers (MDA, H2O2) were enhanced (p &amp;lt; 0.05). In addition, Se deficiency had a positive effect on the pathological changes of inflammation and the exceptional immunity in LPS-treated groups (p &amp;lt; 0.05). The results confirmed the relationship between miR-196-5p and NF\u03baBIA in chickens, revealing that Se deficiency causes respiratory mucosal immune dysfunction via the miR-196-5p-NF\u03baBIA axis, oxidative stress and inflammation. Moreover, Se deficiency exacerbates the inflammatory damage stimulated by LPS. Our work provides a theoretical basis for the prevention of tracheal injury owing to Se deficiency and can be used as a reference for comparative medicine. Furthermore, the targeted regulation of miR-196-5p and NF\u03baBIA may contribute to the protection of the tracheal mucosa in chickens.<\/jats:p>","DOI":"10.1039\/d0mt00164c","type":"journal-article","created":{"date-parts":[[2020,8,31]],"date-time":"2020-08-31T02:03:42Z","timestamp":1598839422000},"page":"1679-1692","update-policy":"https:\/\/doi.org\/10.1039\/rsc_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["MiR-196-5p involvement in selenium deficiency-induced immune damage <i>via<\/i> targeting of NF\u03baBIA in the chicken trachea"],"prefix":"10.1093","volume":"12","author":[{"given":"Linqian","family":"Qin","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, P. R. China. Tel: +86 451 55190083"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yiming","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, P. R. China. Tel: +86 451 55190083"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chunyan","family":"Wan","sequence":"additional","affiliation":[{"name":"National Selenium-rich Product Quality Supervision and Inspection Center, Enshi 445000, P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhu","family":"Wang","sequence":"additional","affiliation":[{"name":"National Selenium-rich Product Quality Supervision and Inspection Center, Enshi 445000, P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yimei","family":"Cong","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, P. R. China. Tel: +86 451 55190083"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0183-5459","authenticated-orcid":false,"given":"Shu","family":"Li","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, P. R. China. Tel: +86 451 55190083"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2020,9,10]]},"reference":[{"key":"2021121004363733400_cit1","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1007\/s12011-016-0651-1","article-title":"Selenium Deficiency-Induced Inflammation and Increased Expression of Regulating Inflammatory Cytokines in the Chicken Gastrointestinal Tract","volume":"173","author":"Gao","year":"2016","journal-title":"Biol. Trace Elem. Res."},{"key":"2021121004363733400_cit2","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1039\/C9MT00006B","article-title":"The regulatory effects of miR-138-5p on selenium deficiency-induced chondrocyte apoptosis are mediated by targeting SelM","volume":"11","author":"Chi","year":"2019","journal-title":"Metallomics"},{"key":"2021121004363733400_cit3","doi-asserted-by":"crossref","first-page":"13693","DOI":"10.1002\/jcp.28050","article-title":"microRNA-33-3p involved in selenium deficiency-induced apoptosis via targeting ADAM10 in the chicken kidney","volume":"234","author":"Wan","year":"2019","journal-title":"J. Cell. Physiol."},{"key":"2021121004363733400_cit4","doi-asserted-by":"crossref","first-page":"e0182079","DOI":"10.1371\/journal.pone.0182079","article-title":"Pancreatic atrophy caused by dietary selenium deficiency induces hypoinsulinemic hyperglycemia via global down-regulation of selenoprotein encoding genes in broilers","volume":"12","author":"Xu","year":"2017","journal-title":"PLoS One"},{"key":"2021121004363733400_cit5","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.redox.2017.11.025","article-title":"miR-200a-5p regulates myocardial necroptosis induced by Se deficiency via targeting RNF11","volume":"15","author":"Yang","year":"2018","journal-title":"Redox Biol."},{"key":"2021121004363733400_cit6","doi-asserted-by":"crossref","first-page":"1203","DOI":"10.3390\/nu10091203","article-title":"Selenium, Selenoproteins, and Immunity","volume":"10","author":"Avery","year":"2018","journal-title":"Nutrients"},{"key":"2021121004363733400_cit7","doi-asserted-by":"crossref","first-page":"660","DOI":"10.1007\/s12011-019-02017-6","article-title":"Selenium Deficiency in Chickens Induces Intestinal Mucosal Injury by Affecting the Mucosa Morphology, SIgA Secretion, and GSH-Px Activity","volume":"197","author":"He","year":"2020","journal-title":"Biol. Trace Elem. Res."},{"key":"2021121004363733400_cit8","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.dci.2017.07.018","article-title":"Selenium accelerates chicken dendritic cells differentiation and affects selenoproteins expression","volume":"77","author":"Sun","year":"2017","journal-title":"Dev. Comp. Immunol."},{"key":"2021121004363733400_cit9","doi-asserted-by":"crossref","first-page":"7198","DOI":"10.1002\/jcp.27473","article-title":"Pre-cold acclimation improves the immune function of trachea and resistance to cold stress in broilers","volume":"234","author":"Su","year":"2019","journal-title":"J. Cell. 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Sci."},{"key":"2021121004363733400_cit19","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1016\/j.chemosphere.2018.05.152","article-title":"HS induces Th1\/Th2 imbalance with triggered NF-kB pathway to exacerbate LPS-induce chicken pneumonia response","volume":"208","author":"Wang","year":"2018","journal-title":"Chemosphere"},{"key":"2021121004363733400_cit20","doi-asserted-by":"crossref","first-page":"3928","DOI":"10.1111\/bph.14465","article-title":"Haem oxygenase-1 up-regulation by rosiglitazone via ROS-dependent Nrf2-antioxidant response elements axis or PPARg attenuates LPS-mediated lung inflammation","volume":"175","author":"Cho","year":"2018","journal-title":"Br. J. 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Sci."},{"key":"2021121004363733400_cit24","doi-asserted-by":"crossref","first-page":"778","DOI":"10.1159\/000495332","article-title":"miR-2954 Inhibits PI3K Signaling and Induces Autophagy and Apoptosis in Myocardium Selenium Deficiency","volume":"51","author":"Liu","year":"2018","journal-title":"Cell. Physiol. Biochem."},{"key":"2021121004363733400_cit25","doi-asserted-by":"crossref","first-page":"1037","DOI":"10.5713\/ajas.16.0685","article-title":"Distribution and differential expression of microRNAs in the intestinal mucosal layer of necrotic enteritis induced Fayoumi chickens","volume":"30","author":"Rengaraj","year":"2017","journal-title":"Asian-Australas. J. Anim. Sci."},{"key":"2021121004363733400_cit26","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1111\/j.1582-4934.2010.01219.x","article-title":"MicroRNA-196: critical roles and clinical applications in development and cancer","volume":"15","author":"Chen","year":"2011","journal-title":"J. Cell. Mol. 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Total Environ."},{"key":"2021121004363733400_cit39","doi-asserted-by":"crossref","first-page":"110993","DOI":"10.1016\/j.ecoenv.2020.110993","article-title":"As or\/and Cu exposure triggers oxidative stress imbalance, induces inflammatory response and apoptosis in chicken brain","volume":"203","author":"Nie","year":"2020","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"2021121004363733400_cit40","doi-asserted-by":"crossref","first-page":"110715","DOI":"10.1016\/j.ecoenv.2020.110715","article-title":"Copper induces oxidative stress with triggered NF-kB pathway leading to inflammatory responses in immune organs of chicken","volume":"200","author":"Yang","year":"2020","journal-title":"Ecotoxicol. Environ. 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Technol."},{"key":"2021121004363733400_cit43","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1186\/s13567-019-0703-6","article-title":"Baicalin mitigated Mycoplasma gallisepticuminduced structural damage and attenuated oxidative stress and apoptosis in chicken thymus through the Nrf2\/HO-1 defence pathway","volume":"50","author":"Li","year":"2019","journal-title":"Vet. Res."},{"key":"2021121004363733400_cit44","doi-asserted-by":"crossref","first-page":"139017","DOI":"10.1016\/j.scitotenv.2020.139017","article-title":"NF-kB pathway took part in the development of apoptosis mediated by miR-15a and oxidative stress via mitochondrial pathway in ammonia-treated chicken splenic lymphocytes","volume":"729","author":"Chen","year":"2020","journal-title":"Sci. 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Res."},{"key":"2021121004363733400_cit47","doi-asserted-by":"crossref","first-page":"4781528","DOI":"10.1155\/2019\/4781528","article-title":"iNOS Inhibition Reduces Lung Mechanical Alterations and Remodeling Induced by Particulate Matter in Mice","volume":"2019","author":"Prado","year":"2019","journal-title":"Pulm. Med."},{"key":"2021121004363733400_cit48","doi-asserted-by":"crossref","first-page":"e01366","DOI":"10.1016\/j.heliyon.2019.e01366","article-title":"Naturally occurring sesquiterpene lactones and their semi-synthetic derivativesmodulate PGE2 levels by decreasing COX2 activity and expression","volume":"5","author":"Perri","year":"2019","journal-title":"Heliyon"}],"container-title":["Metallomics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/metallomics\/article-pdf\/12\/11\/1679\/41701877\/d0mt00164c.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/metallomics\/article-pdf\/12\/11\/1679\/41701877\/d0mt00164c.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,12,10]],"date-time":"2021-12-10T04:49:08Z","timestamp":1639111748000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/metallomics\/article\/12\/11\/1679\/6071916"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,9,10]]},"references-count":48,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2020,11,1]]}},"URL":"https:\/\/doi.org\/10.1039\/d0mt00164c","relation":{},"ISSN":["1756-5901","1756-591X"],"issn-type":[{"type":"print","value":"1756-5901"},{"type":"electronic","value":"1756-591X"}],"subject":[],"published-other":{"date-parts":[[2020,11]]},"published":{"date-parts":[[2020,9,10]]},"assertion":[{"value":"This document is Similarity Check deposited","name":"similarity_check","explanation":{"URL":"https:\/\/www.crossref.org\/services\/similarity-check\/"},"group":{"name":"similarity_check","label":"SIMILARITY CHECK"}},{"value":"Supplementary Information","URL":"https:\/\/doi.org\/10.1039\/D0MT00164C","name":"related_data","group":{"name":"related_data","label":"RELATED DATA"}},{"value":"Shu Li (ORCID)","URL":"http:\/\/orcid.org\/0000-0003-0183-5459","name":"identifier","group":{"name":"author_identifiers","label":"IDENTIFIERS"}},{"value":"Single-blind","order":2,"name":"peer_review_method","group":{"name":"peer_review_method","label":"PEER REVIEW METHOD"}},{"value":"Received 13 July 2020; Accepted 28 August 2020;  Accepted Manuscript published 31 August 2020;  Advance Article published 10 September 2020;  Version of Record published 18 November 2020","order":1,"name":"history","group":{"name":"publication_history","label":"PUBLICATION HISTORY"}}]}}