{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T11:48:57Z","timestamp":1790682537172,"version":"4.1.0"},"reference-count":55,"publisher":"Royal Society of Chemistry (RSC)","issue":"27","license":[{"start":{"date-parts":[[2021,5,4]],"date-time":"2021-05-04T00:00:00Z","timestamp":1620086400000},"content-version":"vor","delay-in-days":123,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100002347","name":"Bundesministerium f\u00fcr Bildung und Forschung","doi-asserted-by":"crossref","award":["German Ministry of Education and Research, BMBF No. 031B0078A"],"award-info":[{"award-number":["German Ministry of Education and Research, BMBF No. 031B0078A"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["rsc.org"],"crossmark-restriction":true},"short-container-title":["RSC Adv."],"abstract":"<p>Carboxylesterase, the key enzyme to handle ester-based dyes, is synthesized in microsomes using eukaryotic cell-free protein synthesis platform and established as a viable calcium imaging tool to analyze native and cell-free synthesized ion channels.<\/p>","DOI":"10.1039\/d0ra08397f","type":"journal-article","created":{"date-parts":[[2021,5,4]],"date-time":"2021-05-04T06:53:46Z","timestamp":1620111226000},"page":"16285-16296","update-policy":"https:\/\/doi.org\/10.1039\/rsc_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Targeted esterase-induced dye (TED) loading supports direct calcium imaging in eukaryotic cell-free systems"],"prefix":"10.1039","volume":"11","author":[{"given":"Priyavathi","family":"Dhandapani","sequence":"first","affiliation":[{"name":"Fraunhofer Institute of Cell Therapy and Immunology"},{"name":"Branch of Bioanalytics and Bioprocesses (IZI-BB)"},{"name":"Potsdam-Golm"},{"name":"Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Srujan Kumar","family":"Dondapati","sequence":"additional","affiliation":[{"name":"Fraunhofer Institute of Cell Therapy and Immunology"},{"name":"Branch of Bioanalytics and Bioprocesses (IZI-BB)"},{"name":"Potsdam-Golm"},{"name":"Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Zemella","sequence":"additional","affiliation":[{"name":"Fraunhofer Institute of Cell Therapy and Immunology"},{"name":"Branch of Bioanalytics and Bioprocesses (IZI-BB)"},{"name":"Potsdam-Golm"},{"name":"Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dennis","family":"Br\u00e4uer","sequence":"additional","affiliation":[{"name":"Fraunhofer Institute of Cell Therapy and Immunology"},{"name":"Branch of Bioanalytics and Bioprocesses (IZI-BB)"},{"name":"Potsdam-Golm"},{"name":"Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Doreen Anja","family":"W\u00fcstenhagen","sequence":"additional","affiliation":[{"name":"Fraunhofer Institute of Cell Therapy and Immunology"},{"name":"Branch of Bioanalytics and Bioprocesses (IZI-BB)"},{"name":"Potsdam-Golm"},{"name":"Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Mergler","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology"},{"name":"Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin"},{"name":"Berlin"},{"name":"Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7238-4117","authenticated-orcid":false,"given":"Stefan","family":"Kubick","sequence":"additional","affiliation":[{"name":"Fraunhofer Institute of Cell Therapy and Immunology"},{"name":"Branch of Bioanalytics and Bioprocesses (IZI-BB)"},{"name":"Potsdam-Golm"},{"name":"Germany"},{"name":"Faculty of Health Sciences"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"292","published-online":{"date-parts":[[2021]]},"reference":[{"issue":"1","key":"D0RA08397F-(cit1)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1038\/nrd.2016.230","volume":"16","author":"Santos","year":"2017","journal-title":"Nat. Rev. Drug Discovery"},{"issue":"17","key":"D0RA08397F-(cit2)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"2420","DOI":"10.1002\/cbic.201500340","volume":"16","author":"Zemella","year":"2015","journal-title":"ChemBioChem"},{"issue":"17","key":"D0RA08397F-(cit3)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"2774","DOI":"10.1016\/j.febslet.2014.06.007","volume":"588","author":"Sachse","year":"2014","journal-title":"FEBS Lett."},{"issue":"3","key":"D0RA08397F-(cit4)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1007\/s40259-020-00417-y","volume":"34","author":"Dondapati","year":"2020","journal-title":"BioDrugs"},{"issue":"3\u20134","key":"D0RA08397F-(cit5)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1016\/j.ceca.2005.06.010","volume":"38","author":"Burdakov","year":"2005","journal-title":"Cell Calcium"},{"issue":"12","key":"D0RA08397F-(cit6)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"1035","DOI":"10.1016\/j.tibs.2016.09.001","volume":"41","author":"Raffaello","year":"2016","journal-title":"Trends Biochem. Sci."},{"issue":"5","key":"D0RA08397F-(cit7)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"e44617","DOI":"10.15252\/embr.201744617","volume":"19","author":"Betzer","year":"2018","journal-title":"EMBO Rep."},{"issue":"1","key":"D0RA08397F-(cit8)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1186\/1480-9222-14-4","volume":"14","author":"McMullen","year":"2012","journal-title":"Biol. Proced. Online"},{"issue":"15","key":"D0RA08397F-(cit9)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"8693","DOI":"10.1073\/pnas.140217897","volume":"97","author":"Navazio","year":"2000","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"1","key":"D0RA08397F-(cit10)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1177\/153537020222700107","volume":"227","author":"Yusufi","year":"2002","journal-title":"Exp. Biol. Med."},{"issue":"1","key":"D0RA08397F-(cit11)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.abb.2007.03.039","volume":"462","author":"Giunti","year":"2007","journal-title":"Arch. Biochem. Biophys."},{"key":"D0RA08397F-(cit12)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1016\/j.neuro.2018.07.015","volume":"69","author":"Dusza","year":"2018","journal-title":"Neurotoxicology"},{"issue":"75","key":"D0RA08397F-(cit13)\/*[position()=1]","first-page":"e50317","author":"Samtleben","year":"2013","journal-title":"J. Visualized Exp."},{"issue":"4","key":"D0RA08397F-(cit14)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1016\/j.ceca.2008.02.002","volume":"44","author":"Rehberg","year":"2008","journal-title":"Cell Calcium"},{"key":"D0RA08397F-(cit15)\/*[position()=1]","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-60761-476-0_8","volume-title":"Calcium Measurement Methods [Internet]","author":"Blum","year":"2010","unstructured":"R. Blum  ,  O. H.Petersen  and  A.Verkhratsky ,  Ca2+ Imaging of Intracellular Organelles: Endoplasmic Reticulum , in  Calcium Measurement Methods [Internet] , ed.  A. Verkhratsky  and  O. H. Petersen ,  Humana Press ,  Totowa, NJ ,  2010 ,  pp. 147\u201367 , (Neuromethods),  10.1007\/978-1-60761-476-0_8"},{"key":"D0RA08397F-(cit16)\/*[position()=1]","first-page":"67","volume":"137","author":"Stech","year":"2013","journal-title":"Adv. Biochem. Eng.\/Biotechnol."},{"issue":"1","key":"D0RA08397F-(cit17)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"3740","DOI":"10.1038\/s41598-017-03955-8","volume":"7","author":"Zemella","year":"2017","journal-title":"Sci. Rep."},{"issue":"12","key":"D0RA08397F-(cit18)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"e82234","DOI":"10.1371\/journal.pone.0082234","volume":"8","author":"Br\u00f6del","year":"2013","journal-title":"PLoS One"},{"issue":"5","key":"D0RA08397F-(cit19)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"e96635","DOI":"10.1371\/journal.pone.0096635","volume":"9","author":"Stech","year":"2014","journal-title":"PLoS One"},{"key":"D0RA08397F-(cit20)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"30399","DOI":"10.1038\/srep30399","volume":"6","author":"Quast","year":"2016","journal-title":"Sci. Rep."},{"issue":"2","key":"D0RA08397F-(cit21)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.ymeth.2004.11.003","volume":"36","author":"Gilham","year":"2005","journal-title":"Methods"},{"issue":"9","key":"D0RA08397F-(cit22)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1016\/j.micres.2013.04.004","volume":"168","author":"Zhu","year":"2013","journal-title":"Microbiol. Res."},{"key":"D0RA08397F-(cit23)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"985","DOI":"10.1186\/s12864-015-2183-z","volume":"16","author":"do Nascimento","year":"2015","journal-title":"BMC Genomics"},{"issue":"1","key":"D0RA08397F-(cit24)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1111\/imb.12140","volume":"24","author":"Giraudo","year":"2015","journal-title":"Insect Mol. Biol."},{"issue":"3","key":"D0RA08397F-(cit25)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/j.ymeth.2008.09.025","volume":"46","author":"Paredes","year":"2008","journal-title":"Methods"},{"issue":"16","key":"D0RA08397F-(cit26)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"10221","DOI":"10.1074\/jbc.R700048200","volume":"283","author":"Caramelo","year":"2008","journal-title":"J. Biol. Chem."},{"issue":"1","key":"D0RA08397F-(cit27)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1161\/01.RES.0000079967.11815.19","volume":"93","author":"Shannon","year":"2003","journal-title":"Circ. Res."},{"issue":"4","key":"D0RA08397F-(cit28)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"2116","DOI":"10.1016\/S0006-3495(00)76758-0","volume":"78","author":"Oliver","year":"2000","journal-title":"Biophys. J."},{"issue":"25","key":"D0RA08397F-(cit29)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"15872","DOI":"10.1074\/jbc.272.25.15872","volume":"272","author":"Autry","year":"1997","journal-title":"J. Biol. Chem."},{"issue":"14","key":"D0RA08397F-(cit30)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"4246","DOI":"10.1021\/bi702089j","volume":"47","author":"Winters","year":"2008","journal-title":"Biochemistry"},{"issue":"1","key":"D0RA08397F-(cit31)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/S0968-0004(97)01143-2","volume":"23","author":"Meldolesi","year":"1998","journal-title":"Trends Biochem. Sci."},{"issue":"3","key":"D0RA08397F-(cit32)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"a004069","DOI":"10.1101\/cshperspect.a004069","volume":"3","author":"Prins","year":"2011","journal-title":"Cold Spring Harbor Perspect. Biol."},{"issue":"5\u20136","key":"D0RA08397F-(cit33)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/S0143416002001884","volume":"32","author":"Michalak","year":"2002","journal-title":"Cell Calcium"},{"issue":"2","key":"D0RA08397F-(cit34)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1085\/jgp.201611624","volume":"149","author":"Uehara","year":"2017","journal-title":"J. Gen. Physiol."},{"key":"D0RA08397F-(cit35)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"101","DOI":"10.3389\/fphar.2014.00101","volume":"5","author":"Camors","year":"2014","journal-title":"Front. Pharmacol."},{"issue":"4","key":"D0RA08397F-(cit36)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"893","DOI":"10.1152\/physrev.00013.2002","volume":"82","author":"Fill","year":"2002","journal-title":"Physiol. Rev."},{"issue":"Pt 12","key":"D0RA08397F-(cit37)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"2483","DOI":"10.1242\/jcs.00455","volume":"116","author":"V\u00e1zquez-Mart\u00ednez","year":"2003","journal-title":"J. Cell Sci."},{"issue":"Pt 24","key":"D0RA08397F-(cit38)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"5899","DOI":"10.1113\/jphysiol.2011.222554","volume":"589","author":"Kunitomo","year":"2011","journal-title":"J. Physiol."},{"key":"D0RA08397F-(cit39)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1186\/1744-8069-2-22","volume":"2","author":"Vetter","year":"2006","journal-title":"Mol. Pain"},{"issue":"1","key":"D0RA08397F-(cit40)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1124\/jpet.300.1.9","volume":"300","author":"Grant","year":"2002","journal-title":"J. Pharmacol. Exp. Ther."},{"issue":"2","key":"D0RA08397F-(cit41)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1111\/j.1471-4159.2010.06626.x","volume":"113","author":"Dong","year":"2010","journal-title":"J. Neurochem."},{"issue":"Pt 2","key":"D0RA08397F-(cit42)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1042\/bj20021574","volume":"372","author":"Wisnoskey","year":"2003","journal-title":"Biochem. J."},{"issue":"2","key":"D0RA08397F-(cit43)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"317","DOI":"10.3390\/cells9020317","volume":"9","author":"Haustrate","year":"2020","journal-title":"Cells"},{"issue":"3","key":"D0RA08397F-(cit44)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"830","DOI":"10.1124\/jpet.107.119412","volume":"321","author":"Thomas","year":"2007","journal-title":"J. Pharmacol. Exp. Ther."},{"issue":"16","key":"D0RA08397F-(cit45)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"10999","DOI":"10.1074\/jbc.M114.553180","volume":"289","author":"Senning","year":"2014","journal-title":"J. Biol. Chem."},{"issue":"24","key":"D0RA08397F-(cit46)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"10246","DOI":"10.1073\/pnas.0703420104","volume":"104","author":"Brauchi","year":"2007","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"2\u20133","key":"D0RA08397F-(cit47)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1007\/s12035-008-8027-y","volume":"37","author":"Rohacs","year":"2008","journal-title":"Mol. Neurobiol."},{"issue":"12","key":"D0RA08397F-(cit48)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"1681","DOI":"10.1042\/BCJ20160069","volume":"473","author":"Kolay","year":"2016","journal-title":"Biochem. J."},{"issue":"1","key":"D0RA08397F-(cit49)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1042\/BJ20090428","volume":"422","author":"Hammond","year":"2009","journal-title":"Biochem. J."},{"key":"D0RA08397F-(cit50)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1007\/978-94-007-3012-0_2","volume":"58","author":"Schramp","year":"2012","journal-title":"Subcell. Biochem."},{"issue":"7","key":"D0RA08397F-(cit51)\/*[position()=1]","first-page":"737","volume":"48","author":"Pentyala","year":"2010","journal-title":"Indian J. Exp. Biol."},{"issue":"1","key":"D0RA08397F-(cit52)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/S0378-4274(99)00009-0","volume":"106","author":"Stoyanovsky","year":"1999","journal-title":"Toxicol. Lett."},{"issue":"2","key":"D0RA08397F-(cit53)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1007\/s11064-007-9430-x","volume":"33","author":"Coburn","year":"2008","journal-title":"Neurochem. Res."},{"issue":"2","key":"D0RA08397F-(cit54)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"952","DOI":"10.1093\/toxsci\/kfi147","volume":"85","author":"Kodavanti","year":"2005","journal-title":"Toxicol. Sci."},{"key":"D0RA08397F-(cit55)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"1347","DOI":"10.1517\/17460441.2015.1091814","volume":"10","author":"Martinez","year":"2015","journal-title":"Expert Opin. Drug Discovery"}],"container-title":["RSC Advances"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/pubs.rsc.org\/en\/content\/articlepdf\/2021\/RA\/D0RA08397F","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T06:11:41Z","timestamp":1782886301000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.rsc.org\/ra\/article\/11\/27\/16285-16296\/703785"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021]]},"references-count":55,"journal-issue":{"issue":"27","published-print":{"date-parts":[[2021,5,4]]}},"URL":"https:\/\/doi.org\/10.1039\/d0ra08397f","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2020.03.05.978247","asserted-by":"object"}]},"ISSN":["2046-2069"],"issn-type":[{"value":"2046-2069","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021]]},"assertion":[{"value":"This document is Similarity Check deposited","name":"similarity_check","explanation":{"URL":"https:\/\/www.crossref.org\/services\/similarity-check\/"},"group":{"name":"similarity_check","label":"SIMILARITY CHECK"}},{"value":"Supplementary Information","URL":"https:\/\/doi.org\/10.1039\/D0RA08397F","name":"related_data","group":{"name":"related_data","label":"RELATED DATA"}},{"value":"Stefan Kubick (ORCID)","URL":"http:\/\/orcid.org\/0000-0002-7238-4117","name":"identifier","group":{"name":"author_identifiers","label":"IDENTIFIERS"}},{"value":"Single-blind","order":2,"name":"peer_review_method","group":{"name":"peer_review_method","label":"PEER REVIEW METHOD"}},{"value":"Received 1 October 2020; Accepted 25 March 2021;  Version of Record published 4 May 2021","order":1,"name":"history","group":{"name":"publication_history","label":"PUBLICATION HISTORY"}}]}}