{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T08:29:25Z","timestamp":1792657765992,"version":"4.5.0"},"reference-count":50,"publisher":"Royal Society of Chemistry (RSC)","issue":"13","license":[{"start":{"date-parts":[[2022,4,12]],"date-time":"2022-04-12T00:00:00Z","timestamp":1649721600000},"content-version":"am","delay-in-days":466,"URL":"http:\/\/rsc.li\/journals-terms-of-use"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["DP2GM140919-01"],"award-info":[{"award-number":["DP2GM140919-01"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100002429","name":"Amgen","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100002429","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100006961","name":"California Institute of Technology","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100006961","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["rsc.org"],"crossmark-restriction":true},"short-container-title":["Analyst"],"abstract":"<p>\n                    High spatial-resolution label-free imaging of\n                    <italic>in vivo<\/italic>\n                    protein aggregates by VISTA for studying the details of amyloid-\u03b2 plaque morphologies was achieved. Highly multiplex volumetric imaging was achieved by coupling VISTA imaging with U-net feature prediction.\n                  <\/p>","DOI":"10.1039\/d1an00060h","type":"journal-article","created":{"date-parts":[[2021,4,12]],"date-time":"2021-04-12T17:04:06Z","timestamp":1618247046000},"page":"4135-4145","update-policy":"https:\/\/doi.org\/10.1039\/rsc_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["High spatial-resolution imaging of label-free\n                    <i>in vivo<\/i>\n                    protein aggregates by VISTA"],"prefix":"10.1039","volume":"146","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3086-6991","authenticated-orcid":false,"given":"Li-En","family":"Lin","sequence":"first","affiliation":[{"name":"Division of Chemistry and Chemical Engineering"},{"name":"California Institute of Technology"},{"name":"Pasadena"},{"name":"USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kun","family":"Miao","sequence":"additional","affiliation":[{"name":"Division of Chemistry and Chemical Engineering"},{"name":"California Institute of Technology"},{"name":"Pasadena"},{"name":"USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4815-5565","authenticated-orcid":false,"given":"Chenxi","family":"Qian","sequence":"additional","affiliation":[{"name":"Division of Chemistry and Chemical Engineering"},{"name":"California Institute of Technology"},{"name":"Pasadena"},{"name":"USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9170-2283","authenticated-orcid":false,"given":"Lu","family":"Wei","sequence":"additional","affiliation":[{"name":"Division of Chemistry and Chemical Engineering"},{"name":"California Institute of Technology"},{"name":"Pasadena"},{"name":"USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"292","published-online":{"date-parts":[[2021]]},"reference":[{"issue":"7","key":"D1AN00060H-(cit1)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"S10","DOI":"10.1038\/nm1066","volume":"10","author":"Ross","year":"2004","journal-title":"Nat. Med."},{"issue":"3","key":"D1AN00060H-(cit2)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1016\/S0092-8674(00)80513-9","volume":"90","author":"Davies","year":"1997","journal-title":"Cell"},{"issue":"5580","key":"D1AN00060H-(cit3)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1126\/science.1072994","volume":"297","author":"Hardy","year":"2002","journal-title":"Science"},{"issue":"6","key":"D1AN00060H-(cit4)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1038\/nchembio.1818","volume":"11","author":"Tanaka","year":"2015","journal-title":"Nat. Chem. Biol."},{"issue":"19 Pt A","key":"D1AN00060H-(cit5)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"2640","DOI":"10.1016\/j.febslet.2015.07.013","volume":"589","author":"Breydo","year":"2015","journal-title":"FEBS Lett."},{"key":"D1AN00060H-(cit6)\/*[position()=1]","volume-title":"Real-time imaging of Huntingtin aggregates diverting target search and gene transcription","volume":"5","author":"Li","year":"2016","unstructured":"L. Li , H.Liu , P.Dong , D.Li , W. R.Legant and J. B.Grimm , et al. , Real-time imaging of Huntingtin aggregates diverting target search and gene transcription , ed. S. C. Kowalczykowski, eLife , 2016 , vol. 5 , pp. e17056"},{"issue":"4","key":"D1AN00060H-(cit7)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"796","DOI":"10.1523\/JNEUROSCI.3501-06.2007","volume":"27","author":"Lacor","year":"2007","journal-title":"J. Neurosci."},{"issue":"1","key":"D1AN00060H-(cit8)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1523\/JNEUROSCI.1698-16.2016","volume":"37","author":"Yang","year":"2017","journal-title":"J. Neurosci."},{"issue":"Suppl 1","key":"D1AN00060H-(cit9)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"iii","DOI":"10.1111\/j.1471-4159.2011.07603.x","volume":"120","author":"Checler","year":"2012","journal-title":"J. Neurochem."},{"issue":"12","key":"D1AN00060H-(cit10)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"951","DOI":"10.1016\/j.tcb.2020.08.007","volume":"30","author":"B\u00e4uerlein","year":"2020","journal-title":"Trends Cell Biol."},{"issue":"8","key":"D1AN00060H-(cit11)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1038\/s41592-018-0053-8","volume":"15","author":"Mlodzianoski","year":"2018","journal-title":"Nat. Methods"},{"issue":"1","key":"D1AN00060H-(cit12)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"5181","DOI":"10.1038\/s41598-019-41443-3","volume":"9","author":"Querol-Vilaseca","year":"2019","journal-title":"Sci. Rep."},{"issue":"1","key":"D1AN00060H-(cit13)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/j.cell.2017.08.009","volume":"171","author":"B\u00e4uerlein","year":"2017","journal-title":"Cell"},{"issue":"4","key":"D1AN00060H-(cit14)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"770","DOI":"10.1016\/j.bpj.2012.12.053","volume":"104","author":"Takasaki","year":"2013","journal-title":"Biophys. J."},{"issue":"12","key":"D1AN00060H-(cit15)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"2767","DOI":"10.1021\/cb500335w","volume":"9","author":"Duim","year":"2014","journal-title":"ACS Chem. Biol."},{"issue":"5","key":"D1AN00060H-(cit16)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"1478","DOI":"10.1074\/jbc.RA118.004511","volume":"294","author":"Lu","year":"2019","journal-title":"J. Biol. Chem."},{"issue":"33","key":"D1AN00060H-(cit17)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"12902","DOI":"10.1021\/ja201651w","volume":"133","author":"Kaminski Schierle","year":"2011","journal-title":"J. Am. Chem. Soc."},{"issue":"42","key":"D1AN00060H-(cit18)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"17828","DOI":"10.1021\/jacs.0c08178","volume":"142","author":"M\u00f6ckl","year":"2020","journal-title":"J. Am. Chem. Soc."},{"issue":"6","key":"D1AN00060H-(cit19)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"E1289","DOI":"10.1073\/pnas.1710329115","volume":"115","author":"Bennett","year":"2018","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"17","key":"D1AN00060H-(cit20)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"10032","DOI":"10.1073\/pnas.1630428100","volume":"100","author":"Gamblin","year":"2003","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"4","key":"D1AN00060H-(cit21)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1136\/jnnp.71.4.441","volume":"71","author":"Du","year":"2001","journal-title":"J. Neurol., Neurosurg. Psychiatry"},{"issue":"6512","key":"D1AN00060H-(cit22)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1126\/science.abb8587","volume":"370","author":"Bartels","year":"2020","journal-title":"Science"},{"issue":"6","key":"D1AN00060H-(cit23)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1038\/s41593-020-0624-8","volume":"23","author":"Habib","year":"2020","journal-title":"Nat. Neurosci."},{"issue":"4","key":"D1AN00060H-(cit24)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"1138","DOI":"10.1016\/j.celrep.2016.06.060","volume":"16","author":"Liebmann","year":"2016","journal-title":"Cell Rep."},{"issue":"10","key":"D1AN00060H-(cit25)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"1518","DOI":"10.1038\/nn.4107","volume":"18","author":"Hama","year":"2015","journal-title":"Nat. Neurosci."},{"issue":"3","key":"D1AN00060H-(cit26)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1007\/s00401-014-1322-y","volume":"128","author":"Ando","year":"2014","journal-title":"Acta Neuropathol."},{"issue":"6","key":"D1AN00060H-(cit27)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"1500","DOI":"10.1016\/j.cell.2015.11.025","volume":"163","author":"Murray","year":"2015","journal-title":"Cell"},{"issue":"1","key":"D1AN00060H-(cit28)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"a006189","DOI":"10.1101\/cshperspect.a006189","volume":"1","author":"Serrano-Pozo","year":"2011","journal-title":"Cold Spring Harbor Perspect. Med."},{"issue":"6815","key":"D1AN00060H-(cit29)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"975","DOI":"10.1038\/35050103","volume":"408","author":"Chen","year":"2000","journal-title":"Nature"},{"issue":"22","key":"D1AN00060H-(cit30)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"13990","DOI":"10.1073\/pnas.222433299","volume":"99","author":"Urbanc","year":"2002","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"15","key":"D1AN00060H-(cit31)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"4967","DOI":"10.1039\/C5AN00342C","volume":"140","author":"Kurouski","year":"2015","journal-title":"Analyst"},{"issue":"1","key":"D1AN00060H-(cit32)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1146\/annurev-biophys-052118-115500","volume":"48","author":"Shen","year":"2019","journal-title":"Annu. Rev. Biophys."},{"issue":"6009","key":"D1AN00060H-(cit33)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"1368","DOI":"10.1126\/science.1197236","volume":"330","author":"Saar","year":"2010","journal-title":"Science"},{"issue":"6264","key":"D1AN00060H-(cit34)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"aaa8870","DOI":"10.1126\/science.aaa8870","volume":"350","author":"Cheng","year":"2015","journal-title":"Science"},{"issue":"11","key":"D1AN00060H-(cit35)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"eaat7715","DOI":"10.1126\/sciadv.aat7715","volume":"4","author":"Ji","year":"2018","journal-title":"Sci. Adv."},{"key":"D1AN00060H-(cit36)\/*[position()=1]","doi-asserted-by":"publisher","DOI":"10.1101\/2021.01.08.425961","author":"Qian","year":"2021","journal-title":"bioRxiv"},{"issue":"1","key":"D1AN00060H-(cit37)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1038\/s41592-018-0219-4","volume":"16","author":"Wassie","year":"2019","journal-title":"Nat. Methods"},{"issue":"11","key":"D1AN00060H-(cit38)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1038\/s41592-018-0111-2","volume":"15","author":"Ounkomol","year":"2018","journal-title":"Nat. Methods"},{"issue":"40","key":"D1AN00060H-(cit39)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"10129","DOI":"10.1523\/JNEUROSCI.1202-06.2006","volume":"26","author":"Oakley","year":"2006","journal-title":"J. Neurosci."},{"key":"D1AN00060H-(cit40)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"13489","DOI":"10.1038\/srep13489","volume":"5","author":"Kiskis","year":"2015","journal-title":"Sci. Rep."},{"issue":"9","key":"D1AN00060H-(cit41)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"973","DOI":"10.1038\/nbt.3641","volume":"34","author":"Ku","year":"2016","journal-title":"Nat. Biotechnol."},{"key":"D1AN00060H-(cit42)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"114","DOI":"10.3389\/fnagi.2018.00114","volume":"10","author":"Perez-Nievas","year":"2018","journal-title":"Front. Aging Neurosci."},{"issue":"2","key":"D1AN00060H-(cit43)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1006\/exnr.1997.6738","volume":"149","author":"DeWitt","year":"1998","journal-title":"Exp. Neurol."},{"issue":"1","key":"D1AN00060H-(cit44)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1186\/s13195-015-0104-7","volume":"7","author":"Chui","year":"2015","journal-title":"Alzheimer's Res. Ther."},{"issue":"1","key":"D1AN00060H-(cit45)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/978-3-031-01821-3","volume":"8","author":"Khan","year":"2018","journal-title":"Synth. Lect. Comput. Vis."},{"key":"D1AN00060H-(cit46)\/*[position()=1]","author":"Ronneberger","unstructured":"O. Ronneberger , P.Fischer and T.Brox , U-Net: Convolutional Networks for Biomedical Image Segmentation. 2015 May 18 [cited 2020 Sep 25]; available from: https:\/\/arxiv.org\/abs\/1505.04597v1"},{"issue":"8","key":"D1AN00060H-(cit47)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"3860","DOI":"10.1364\/BOE.10.003860","volume":"10","author":"Manifold","year":"2019","journal-title":"Biomed. Opt. Express"},{"issue":"7638","key":"D1AN00060H-(cit48)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1038\/nature21029","volume":"541","author":"Liddelow","year":"2017","journal-title":"Nature"},{"issue":"6","key":"D1AN00060H-(cit49)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1038\/nrn3880","volume":"16","author":"Heppner","year":"2015","journal-title":"Nat. Rev. Neurosci."},{"issue":"7","key":"D1AN00060H-(cit50)\/*[position()=1]","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1038\/nm1058","volume":"10","author":"Koistinaho","year":"2004","journal-title":"Nat. Med."}],"container-title":["The Analyst"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/pubs.rsc.org\/en\/content\/articlepdf\/2021\/AN\/D1AN00060H","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T05:10:31Z","timestamp":1782882631000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.rsc.org\/an\/article\/146\/13\/4135-4145\/714321"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021]]},"references-count":50,"journal-issue":{"issue":"13","published-print":{"date-parts":[[2021,6,28]]}},"URL":"https:\/\/doi.org\/10.1039\/d1an00060h","relation":{},"ISSN":["0003-2654","1364-5528"],"issn-type":[{"value":"0003-2654","type":"print"},{"value":"1364-5528","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021]]},"assertion":[{"value":"This document is Similarity Check deposited","name":"similarity_check","explanation":{"URL":"https:\/\/www.crossref.org\/services\/similarity-check\/"},"group":{"name":"similarity_check","label":"SIMILARITY CHECK"}},{"value":"Supplementary Information","URL":"https:\/\/doi.org\/10.1039\/D1AN00060H","name":"related_data","group":{"name":"related_data","label":"RELATED DATA"}},{"value":"Li-En Lin (ORCID)","URL":"http:\/\/orcid.org\/0000-0003-3086-6991","name":"identifier","group":{"name":"author_identifiers","label":"IDENTIFIERS"}},{"value":"Chenxi Qian (ORCID)","URL":"http:\/\/orcid.org\/0000-0003-4815-5565","name":"identifier","group":{"name":"author_identifiers","label":"IDENTIFIERS"}},{"value":"Lu Wei (ORCID)","URL":"http:\/\/orcid.org\/0000-0001-9170-2283","name":"identifier","group":{"name":"author_identifiers","label":"IDENTIFIERS"}},{"value":"Single-blind","order":2,"name":"peer_review_method","group":{"name":"peer_review_method","label":"PEER REVIEW METHOD"}},{"value":"Received 11 January 2021; Accepted 7 April 2021; Accepted Manuscript published 12 April 2021; Advance Article published 5 May 2021; Version of Record published 28 June 2021","order":1,"name":"history","group":{"name":"publication_history","label":"PUBLICATION HISTORY"}}]}}