{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T15:51:57Z","timestamp":1787673117654,"version":"build-2736575974"},"reference-count":23,"publisher":"Royal Society of Chemistry (RSC)","issue":"4","license":[{"start":{"date-parts":[[2022,12,28]],"date-time":"2022-12-28T00:00:00Z","timestamp":1672185600000},"content-version":"am","delay-in-days":361,"URL":"http:\/\/rsc.li\/journals-terms-of-use"}],"funder":[{"DOI":"10.13039\/501100003399","name":"Science and Technology Commission of Shanghai Municipality","doi-asserted-by":"publisher","award":["18441900400"],"award-info":[{"award-number":["18441900400"]}],"id":[{"id":"10.13039\/501100003399","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003399","name":"Science and Technology Commission of Shanghai Municipality","doi-asserted-by":"publisher","award":["19ZR1477500"],"award-info":[{"award-number":["19ZR1477500"]}],"id":[{"id":"10.13039\/501100003399","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008050","name":"University of Shanghai for Science and Technology","doi-asserted-by":"publisher","award":["2017KJFZ049"],"award-info":[{"award-number":["2017KJFZ049"]}],"id":[{"id":"10.13039\/501100008050","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["rsc.org"],"crossmark-restriction":true},"short-container-title":["Lab Chip"],"abstract":"<jats:p>The concept of time to place conversion makes using a continuous flow polymerase chain reaction microfluidic chip an ideal way to reduce the time required for amplification of target genes; however, it also brings about low throughput amplicons.<\/jats:p>","DOI":"10.1039\/d1lc00814e","type":"journal-article","created":{"date-parts":[[2021,12,28]],"date-time":"2021-12-28T07:41:03Z","timestamp":1640677263000},"page":"733-737","update-policy":"https:\/\/doi.org\/10.1039\/rsc_crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["A continuous flow PCR array microfluidic chip applied for simultaneous amplification of target genes of periodontal pathogens"],"prefix":"10.1039","volume":"22","author":[{"given":"Bo","family":"Yang","sequence":"first","affiliation":[{"name":"Engineering Research Center of Optical Instrument and System, Key Lab of Optical Instruments and Equipment for Medical Engineering, Ministry of Education, Shanghai Key Lab of Modern Optical System, University of Shanghai for Science and Technology, Shanghai 200093, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, 1665 Kongjiang Road, Shanghai 200092, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9060-7305","authenticated-orcid":false,"given":"Zhenqing","family":"Li","sequence":"additional","affiliation":[{"name":"Engineering Research Center of Optical Instrument and System, Key Lab of Optical Instruments and Equipment for Medical Engineering, Ministry of Education, Shanghai Key Lab of Modern Optical System, University of Shanghai for Science and Technology, Shanghai 200093, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunxian","family":"Tao","sequence":"additional","affiliation":[{"name":"Engineering Research Center of Optical Instrument and System, Key Lab of Optical Instruments and Equipment for Medical Engineering, Ministry of Education, Shanghai Key Lab of Modern Optical System, University of Shanghai for Science and Technology, Shanghai 200093, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingxiang","family":"You","sequence":"additional","affiliation":[{"name":"Engineering Research Center of Optical Instrument and System, Key Lab of Optical Instruments and Equipment for Medical Engineering, Ministry of Education, Shanghai Key Lab of Modern Optical System, University of Shanghai for Science and Technology, Shanghai 200093, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4160-5578","authenticated-orcid":false,"given":"Shinichi","family":"Sekine","sequence":"additional","affiliation":[{"name":"Department of Preventive Dentistry, Graduate School of Dentistry, Osaka University, Osaka 565-0871, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Songlin","family":"Zhuang","sequence":"additional","affiliation":[{"name":"Engineering Research Center of Optical Instrument and System, Key Lab of Optical Instruments and Equipment for Medical Engineering, Ministry of Education, Shanghai Key Lab of Modern Optical System, University of Shanghai for Science and Technology, Shanghai 200093, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0841-7826","authenticated-orcid":false,"given":"Dawei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Engineering Research Center of Optical Instrument and System, Key Lab of Optical Instruments and Equipment for Medical Engineering, Ministry of Education, Shanghai Key Lab of Modern Optical System, University of Shanghai for Science and Technology, Shanghai 200093, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshinori","family":"Yamaguchi","sequence":"additional","affiliation":[{"name":"Oono Joint Research laboratory, Graduate School of Engineering, Osaka University, Osaka 565-0871, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"292","published-online":{"date-parts":[[2022]]},"reference":[{"key":"D1LC00814E\/cit1\/1","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1038\/sj.ebd.6400925","volume":"14","author":"Richards","year":"2013","journal-title":"Evid. Based Dent."},{"key":"D1LC00814E\/cit2\/1","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.bj.2018.12.001","volume":"42","author":"Bui","year":"2019","journal-title":"Biomed. J."},{"key":"D1LC00814E\/cit3\/1","doi-asserted-by":"crossref","first-page":"683","DOI":"10.3892\/mco.2017.1220","volume":"6","author":"Maruyama","year":"2017","journal-title":"Mol. Clin. Oncol."},{"key":"D1LC00814E\/cit4\/1","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1111\/omi.12201","volume":"33","author":"Gualtero","year":"2018","journal-title":"Mol. Oral Microbiol."},{"key":"D1LC00814E\/cit5\/1","doi-asserted-by":"crossref","first-page":"e1003933","DOI":"10.1371\/journal.ppat.1003933","volume":"10","author":"Whitmore","year":"2014","journal-title":"PLoS Pathog."},{"key":"D1LC00814E\/cit6\/1","doi-asserted-by":"crossref","first-page":"111","DOI":"10.47102\/annals-acadmedsg.V34N1p111","volume":"34","author":"Yeo","year":"2005","journal-title":"Ann. Acad. Med. Singap."},{"key":"D1LC00814E\/cit7\/1","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1007\/s10266-006-0060-6","volume":"94","author":"Kim","year":"2006","journal-title":"Odontology"},{"key":"D1LC00814E\/cit8\/1","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1111\/j.1600-0757.2005.00107.x","volume":"38","author":"Socransky","year":"2005","journal-title":"Periodontology 2000"},{"key":"D1LC00814E\/cit9\/1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.anaerobe.2017.10.011","volume":"49","author":"Gita","year":"2018","journal-title":"Anaerobe"},{"key":"D1LC00814E\/cit10\/1","first-page":"70","volume":"10","author":"Su","year":"2019","journal-title":"Biomed. Rep."},{"key":"D1LC00814E\/cit11\/1","doi-asserted-by":"crossref","first-page":"261","DOI":"10.5051\/jpis.2018.48.4.261","volume":"48","author":"Choi","year":"2018","journal-title":"J. Periodontal Implant Sci."},{"key":"D1LC00814E\/cit12\/1","doi-asserted-by":"crossref","first-page":"127857","DOI":"10.1016\/j.foodchem.2020.127857","volume":"339","author":"Zhao","year":"2021","journal-title":"Food Chem."},{"key":"D1LC00814E\/cit13\/1","doi-asserted-by":"crossref","first-page":"1046","DOI":"10.1126\/science.280.5366.1046","volume":"280","author":"Kopp","year":"1998","journal-title":"Science"},{"key":"D1LC00814E\/cit14\/1","doi-asserted-by":"crossref","first-page":"544","DOI":"10.1016\/j.talanta.2017.07.085","volume":"176","author":"Trinh","year":"2018","journal-title":"Talanta"},{"key":"D1LC00814E\/cit15\/1","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1016\/j.snb.2014.09.004","volume":"206","author":"Tachibana","year":"2015","journal-title":"Sens. Actuators, B"},{"key":"D1LC00814E\/cit16\/1","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1007\/s10544-019-0457-y","volume":"22","author":"Li","year":"2020","journal-title":"Biomed. Microdevices"},{"key":"D1LC00814E\/cit17\/1","doi-asserted-by":"crossref","first-page":"2663","DOI":"10.1039\/C9LC00305C","volume":"19","author":"Li","year":"2019","journal-title":"Lab Chip"},{"key":"D1LC00814E\/cit18\/1","doi-asserted-by":"crossref","first-page":"3159","DOI":"10.1039\/D1LC00457C","volume":"21","author":"Li","year":"2021","journal-title":"Lab Chip"},{"key":"D1LC00814E\/cit19\/1","doi-asserted-by":"crossref","first-page":"6923","DOI":"10.1007\/s00216-015-8703-5","volume":"407","author":"Durney","year":"2015","journal-title":"Anal. Bioanal. Chem."},{"key":"D1LC00814E\/cit20\/1","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1111\/jcpe.12087","volume":"40","author":"Bizzarro","year":"2013","journal-title":"J. Clin. Periodontol."},{"key":"D1LC00814E\/cit21\/1","doi-asserted-by":"crossref","first-page":"3334","DOI":"10.1128\/JCM.40.9.3334-3340.2002","volume":"40","author":"Asai","year":"2002","journal-title":"J. Clin. Microbiol."},{"key":"D1LC00814E\/cit22\/1","doi-asserted-by":"crossref","first-page":"109","DOI":"10.2209\/tdcpublication.41.109","volume":"41","author":"Kasuga","year":"2000","journal-title":"Bull. Tokyo Dent. Coll."},{"key":"D1LC00814E\/cit23\/1","first-page":"65","volume":"1099","author":"Waters","year":"2014","journal-title":"J. Cereal Sci."}],"container-title":["Lab on a Chip"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/pubs.rsc.org\/en\/content\/articlepdf\/2022\/LC\/D1LC00814E","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T06:26:42Z","timestamp":1782887202000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.rsc.org\/lc\/article\/22\/4\/733-737\/730773"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022]]},"references-count":23,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2022,2,15]]}},"URL":"https:\/\/doi.org\/10.1039\/d1lc00814e","relation":{},"ISSN":["1473-0197","1473-0189"],"issn-type":[{"value":"1473-0197","type":"print"},{"value":"1473-0189","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022]]}}}