{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T23:20:00Z","timestamp":1767914400340,"version":"3.49.0"},"reference-count":46,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2021,6,8]],"date-time":"2021-06-08T00:00:00Z","timestamp":1623110400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"},{"start":{"date-parts":[[2022,6,8]],"date-time":"2022-06-08T00:00:00Z","timestamp":1654646400000},"content-version":"am","delay-in-days":365,"URL":"http:\/\/rsc.li\/journals-terms-of-use"}],"funder":[{"DOI":"10.13039\/501100013066","name":"Key Scientific Research Project of Colleges and Universities in Henan Province","doi-asserted-by":"publisher","award":["20A230006"],"award-info":[{"award-number":["20A230006"]}],"id":[{"id":"10.13039\/501100013066","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013066","name":"Key Scientific Research Project of Colleges and Universities in Henan Province","doi-asserted-by":"publisher","award":["20A230006"],"award-info":[{"award-number":["20A230006"]}],"id":[{"id":"10.13039\/501100013066","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["rsc.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2021,6,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Valgus\u2013varus Deformity (VVD) leg disease often affects chickens raised in modern large-scale breeding operations. Losses due to VVD are costly to farmers, and the condition also causes significant suffering in affected birds. In this study, we profiled RNAs from the spleens of VVD (BS) and healthy (JS) broilers using high-throughput sequencing to identify miRNAs that might be involved in the development of the disease. Fifty differentially expressed miRNAs (DEMs) were found, of which 30 were up-regulated and 20 were down-regulated in VVD-affected birds (|log\u20092 Fold Change| \u2265 1 and q-value &amp;lt; 0.05). DEMs were matched with putative target genes and 864 target genes were found. Gene Ontology (GO) analyses of these target genes showed that they were significantly enriched in the \u201ccytoplasm\u201d term (q-value &amp;lt; 0.05), and most of the target genes were enriched in \u201ccellular component\u201d. Kyoto encyclopedia of genes and genomes (KEGG) analysis showed that they were significantly enriched in 11 signaling pathways (P-value &amp;lt; 0.05), including metabolic pathways, 2-oxocarboxylic acid metabolism, regulation of actin cytoskeleton, purine metabolism, endocytosis and so on. And we found that they were enriched in immune-related pathways in which MAPK, Notch, JAK-Stat, Toll-like receptor, p53 and other single pathways were involved in the development of skeletal diseases. Differentially expressed mRNAs obtained from our previous study were used to construct an interaction network consisting of 16 DEMs and 21 differentially expressed mRNAs (|log\u20092 Fold Change| \u2265 1 and q-value &amp;lt; 0.05). We found that miR-12247-5p, miR-15c-5p, miR-15b-5p, FKBP5 and HSP90AB1 were at the center of network interaction. This study provides a foundation for further investigations of the pathogenesis and genetic mechanisms underlying VVD.<\/jats:p>\n                  <jats:p\/>","DOI":"10.1039\/d1mo00011j","type":"journal-article","created":{"date-parts":[[2021,6,7]],"date-time":"2021-06-07T22:23:41Z","timestamp":1623104621000},"page":"752-759","update-policy":"https:\/\/doi.org\/10.1039\/rsc_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Identification of key miRNAs affecting broilers with valgus\u2013varus deformity by RNA sequencing and analysis of miRNA\u2013mRNA interactions"],"prefix":"10.1093","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9212-0485","authenticated-orcid":false,"given":"Zhenzhen","family":"Zhang","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology, Henan Agricultural University a , Zhengzhou, China \u00a0 rlhan@126.com \u00a0 xtkang2001@263.net"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hehe","family":"Tang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Henan Agricultural University a , Zhengzhou, China \u00a0 rlhan@126.com \u00a0 xtkang2001@263.net"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanchao","family":"Ma","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Henan Agricultural University a , Zhengzhou, China \u00a0 rlhan@126.com \u00a0 xtkang2001@263.net"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianzeng","family":"Li","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Henan Agricultural University a , Zhengzhou, China \u00a0 rlhan@126.com \u00a0 xtkang2001@263.net"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhuanjian","family":"Li","sequence":"additional","affiliation":[{"name":"Henan Innovative Engineering Research Center of Poultry Germplasm Resource b , Zhengzhou, China"},{"name":"College of Animal Science and Technology, Henan Agricultural University a , Zhengzhou, China \u00a0 rlhan@126.com \u00a0 xtkang2001@263.net"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanhua","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Henan Agricultural University a , Zhengzhou, China \u00a0 rlhan@126.com \u00a0 xtkang2001@263.net"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuanfang","family":"Li","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Henan Agricultural University a , Zhengzhou, China \u00a0 rlhan@126.com \u00a0 xtkang2001@263.net"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiangtao","family":"Kang","sequence":"additional","affiliation":[{"name":"Henan Innovative Engineering Research Center of Poultry Germplasm Resource b , Zhengzhou, China"},{"name":"College of Animal Science and Technology, Henan Agricultural University a , Zhengzhou, China \u00a0 rlhan@126.com \u00a0 xtkang2001@263.net"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3695-1647","authenticated-orcid":false,"given":"Ruili","family":"Han","sequence":"additional","affiliation":[{"name":"Henan Innovative Engineering Research Center of Poultry Germplasm Resource b , Zhengzhou, China"},{"name":"College of Animal Science and Technology, Henan Agricultural University a , Zhengzhou, China \u00a0 rlhan@126.com \u00a0 xtkang2001@263.net"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2021,6,8]]},"reference":[{"key":"2026010813083457400_cit1","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1080\/03079459208418861","volume":"21","author":"Leterrier","year":"1992","journal-title":"Avian Pathol."},{"key":"2026010813083457400_cit2","doi-asserted-by":"crossref","first-page":"45","DOI":"10.3184\/147020602783698421","volume":"13","author":"Bradshaw","year":"2002","journal-title":"Avian Poultry Biol. 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Rep."},{"key":"2026010813083457400_cit24","first-page":"57","author":"Chowdhury","year":"2008","journal-title":"Inflammation Res."},{"key":"2026010813083457400_cit25","doi-asserted-by":"crossref","first-page":"1198","DOI":"10.1242\/dev.070649","volume":"139","author":"Kohn","year":"2012","journal-title":"Development"},{"key":"2026010813083457400_cit26","first-page":"74","author":"Park","year":"2015","journal-title":"Ann. Rheum. Dis."},{"key":"2026010813083457400_cit27","doi-asserted-by":"crossref","first-page":"4073","DOI":"10.1128\/MCB.22.12.4073-4085.2002","volume":"22","author":"Buchsbaum","year":"2002","journal-title":"Mol. Cell. Biol."},{"key":"2026010813083457400_cit28","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1016\/j.coph.2011.03.013","volume":"11","author":"Cioffi","year":"2011","journal-title":"Curr. Opin. Pharmacol."},{"key":"2026010813083457400_cit29","first-page":"35","volume":"48","author":"Hehe","year":"2021","journal-title":"China Anim. Husb. Vet. 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Pathol."},{"key":"2026010813083457400_cit35","doi-asserted-by":"crossref","first-page":"4433","DOI":"10.3382\/ps\/pez269","volume":"98","author":"Guo","year":"2019","journal-title":"Poult. Sci."},{"key":"2026010813083457400_cit36","doi-asserted-by":"crossref","first-page":"1619","DOI":"10.3382\/ps.2012-02207","volume":"91","author":"Genin","year":"2012","journal-title":"Poult. Sci."},{"key":"2026010813083457400_cit37","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1007\/s10456-013-9345-x","volume":"16","author":"Miwa","year":"2013","journal-title":"Angiogenesis"},{"key":"2026010813083457400_cit38","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1007\/s10585-006-9038-6","volume":"23","author":"Menu","year":"2006","journal-title":"Clin. Exp. Metastasis"},{"key":"2026010813083457400_cit39","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1002\/ajmg.c.31747","volume":"181","author":"Zenker","year":"2019","journal-title":"Am. J. Med. 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Physiol."},{"key":"2026010813083457400_cit44","first-page":"28","author":"Bihan-Duval","year":"1996","journal-title":"Genet., Sel., Evol."},{"key":"2026010813083457400_cit45","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1111\/j.1439-0388.1997.tb00510.x","volume":"114","author":"Le Bihan-Duval","year":"1997","journal-title":"J. Anim. Breed. 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