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Metabolic phenotypes in macrophages have been associated with functional phenotypes, where pro-inflammatory macrophages have an increased rate of glycolysis and anti-inflammatory macrophages primarily use oxidative phosphorylation. \u03b2-adrenoceptor (\u03b2AR) signalling in macrophages has been implicated in disease states such as cancer, atherosclerosis and rheumatoid arthritis. The impact of \u03b2AR signalling on macrophage metabolism has not been defined. Using metabolomics and proteomics, we describe the impact of \u03b2AR signalling on macrophages treated with isoprenaline. We found that \u03b2AR signalling alters proteins involved in cytoskeletal rearrangement and redox homeostasis of the cell. We showed that \u03b2AR signalling in macrophages shifts glucose metabolism from glycolysis towards the tricarboxylic acid cycle and pentose phosphate pathways. We also show that \u03b2AR signalling perturbs purine metabolism by accumulating adenylate and guanylate pools. Taken together, these results indicate that \u03b2AR signalling shifts metabolism to support redox processes and upregulates proteins involved in cytoskeletal changes, which may contribute to \u03b2AR effects on macrophage function.<\/jats:p>\n                  <jats:p\/>","DOI":"10.1039\/d1mo00057h","type":"journal-article","created":{"date-parts":[[2021,6,3]],"date-time":"2021-06-03T21:44:00Z","timestamp":1622756640000},"page":"583-595","update-policy":"https:\/\/doi.org\/10.1039\/rsc_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["\u03b2-Adrenoceptor regulation of metabolism in U937 derived macrophages"],"prefix":"10.1093","volume":"17","author":[{"given":"Amanda L","family":"Peterson","sequence":"first","affiliation":[{"name":"Drug Delivery, Disposition and Dynamics Theme, Monash Institute of Pharmaceutical Science, Monash University a , Parkville, Victoria 3052, Australia \u00a0 darren.creek@monash.edu \u00a0 +61-3-9903-9249"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ghizal","family":"Siddiqui","sequence":"additional","affiliation":[{"name":"Drug Delivery, Disposition and Dynamics Theme, Monash Institute of Pharmaceutical Science, Monash University a , Parkville, Victoria 3052, Australia \u00a0 darren.creek@monash.edu \u00a0 +61-3-9903-9249"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erica K","family":"Sloan","sequence":"additional","affiliation":[{"name":"Cousins Center for PNI, Semel Institute, UCLA Jonsson Comprehensive Cancer Center, and the UCLA AIDS Institute, University of California Los Angeles d , Los Angeles, CA, 90095, USA"},{"name":"Division of Cancer Surgery, Peter MacCallum Cancer Centre c , Melbourne, Victoria 3000, Australia"},{"name":"Drug Discovery Biology Theme, Monash Institute of Pharmaceutical Science, Monash University b , Parkville, Victoria 3052, Australia \u00a0 erica.sloan@monash.edu"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7497-7082","authenticated-orcid":false,"given":"Darren J","family":"Creek","sequence":"additional","affiliation":[{"name":"Drug Delivery, Disposition and Dynamics Theme, Monash Institute of Pharmaceutical Science, Monash University a , Parkville, Victoria 3052, Australia \u00a0 darren.creek@monash.edu \u00a0 +61-3-9903-9249"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2021,6,4]]},"reference":[{"key":"2026010813085284100_cit1","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1016\/j.immuni.2015.02.005","volume":"42","author":"Jha","year":"2015","journal-title":"Immunity"},{"key":"2026010813085284100_cit2","doi-asserted-by":"crossref","first-page":"1462","DOI":"10.3389\/fimmu.2019.01462","volume":"10","author":"Viola","year":"2019","journal-title":"Front. 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