{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T22:06:26Z","timestamp":1792101986986,"version":"4.4.0"},"reference-count":26,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2021,11,24]],"date-time":"2021-11-24T00:00:00Z","timestamp":1637712000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"},{"start":{"date-parts":[[2022,11,24]],"date-time":"2022-11-24T00:00:00Z","timestamp":1669248000000},"content-version":"am","delay-in-days":365,"URL":"http:\/\/rsc.li\/journals-terms-of-use"}],"funder":[{"DOI":"10.13039\/501100010877","name":"Science, Technology and Innovation Commission of Shenzhen Municipality","doi-asserted-by":"publisher","award":["KQJSCX20180327124222443"],"award-info":[{"award-number":["KQJSCX20180327124222443"]}],"id":[{"id":"10.13039\/501100010877","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2018A030313669"],"award-info":[{"award-number":["2018A030313669"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010877","name":"Science, Technology and Innovation Commission of Shenzhen Municipality","doi-asserted-by":"publisher","award":["KQJSCX20180327124222443"],"award-info":[{"award-number":["KQJSCX20180327124222443"]}],"id":[{"id":"10.13039\/501100010877","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2018A030313669"],"award-info":[{"award-number":["2018A030313669"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["rsc.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2021,11,24]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Asthenozoospermia (AZS), diagnosed by reduced sperm motility, is one of the major causes of male infertility. However, AZS has no effective therapeutic treatment and the underlying molecular mechanism remains largely unclear. In this study, state-of-the-art 4D-quantitative proteomics analysis was used to compare the protein profiling between 7 normozoospermic and 11 asthenozoospermic sperm samples. Overall, 4718 proteins were identified and 1430 differential abundant proteins were found in the two groups. The differentially expressed proteins were analyzed by GO and KEGG. The core deregulated proteins and pathways associated sperm motility dysfunction included energy metabolism and the sperm structure. Integrative analysis further identified extracellular matrix protein 1 (ECM1) as a novel biomarker related to AZS. Our study could provide new insights into the molecular basis of low sperm motility. The mass spectrometric data are available via ProteomeXchange with identifier PXD027637.<\/jats:p>\n                  <jats:p\/>","DOI":"10.1039\/d1mo00257k","type":"journal-article","created":{"date-parts":[[2021,11,24]],"date-time":"2021-11-24T06:40:15Z","timestamp":1637736015000},"page":"83-91","update-policy":"https:\/\/doi.org\/10.1039\/rsc_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["4D-quantitative proteomics signature of asthenozoospermia and identification of extracellular matrix protein 1 as a novel biomarker for sperm motility"],"prefix":"10.1093","volume":"18","author":[{"given":"Jie","family":"Yang","sequence":"first","affiliation":[{"name":"Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Institute of Urology, Peking University Shenzhen Hospital, Shenzhen PKU-HKUST Medical Center a , Shenzhen, 518036, P. R. China \u00a0 yangbo@sphmc.org \u00a0 +86 755 83923333-3320 \u00a0 +86 755 83923333-3419"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qunlong","family":"Liu","sequence":"additional","affiliation":[{"name":"Reproductive Medicine Center, Peking University Shenzhen Hospital b , P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bohai","family":"Yu","sequence":"additional","affiliation":[{"name":"Medical Laboratory Department, Shenzhen Hospital (Futian) of Guangzhou University of Chinese Medicine c , P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baoquan","family":"Han","sequence":"additional","affiliation":[{"name":"Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Institute of Urology, Peking University Shenzhen Hospital, Shenzhen PKU-HKUST Medical Center a , Shenzhen, 518036, P. R. China \u00a0 yangbo@sphmc.org \u00a0 +86 755 83923333-3320 \u00a0 +86 755 83923333-3419"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6819-0339","authenticated-orcid":false,"given":"Bo","family":"Yang","sequence":"additional","affiliation":[{"name":"Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Institute of Urology, Peking University Shenzhen Hospital, Shenzhen PKU-HKUST Medical Center a , Shenzhen, 518036, P. R. China \u00a0 yangbo@sphmc.org \u00a0 +86 755 83923333-3320 \u00a0 +86 755 83923333-3419"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2021,11,24]]},"reference":[{"issue":"5","key":"2026010813065811900_cit1","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1080\/01485010390219656","article-title":". Asthenozoospermia: analysis of a large population","volume":"49","author":"Curi","year":"2003","journal-title":"Arch. 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