{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T15:44:14Z","timestamp":1785512654598,"version":"3.56.0"},"reference-count":41,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2021,12,16]],"date-time":"2021-12-16T00:00:00Z","timestamp":1639612800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"},{"start":{"date-parts":[[2022,12,16]],"date-time":"2022-12-16T00:00:00Z","timestamp":1671148800000},"content-version":"am","delay-in-days":365,"URL":"http:\/\/rsc.li\/journals-terms-of-use"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81673117"],"award-info":[{"award-number":["81673117"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81673117"],"award-info":[{"award-number":["81673117"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["rsc.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2021,12,16]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Kashin-Beck disease (KBD) is a chronic, endemic and deforming osteochondropathy, whose basic pathological alterations include apoptosis and necrosis of chondrocytes in articular cartilage and growth plates and imbalanced extracellular matrix metabolism. Numerous studies have reported that long noncoding RNAs (lncRNAs) and microRNA (miRNAs) are aberrantly expressed in KBD. Our study was comprised of 5 KBD patients and 5 healthy individuals and we compared the expression profiles of mRNAs, lncRNAs and miRNAs through RNA-sequencing (RNA-seq). Bioinformatic analysis of GO and KEGG was employed to conduct functional annotation and pathway enriched analysis. In total, 3194 mRNAs, 4103 lncRNAs and 1550 miRNAs were detected to be differentially expressed by RNA-seq (P &amp;lt; 0.05; |log2FC| \u22651). The lysosome pathway, Wnt signaling pathway, TNF signaling pathway, endocytosis and mTOR signaling pathway were identified to be involved in the KBD development according to the result of the KEGG analysis. In addition, a ceRNA network based on lncRNA\u2013miRNA\u2013mRNA was constructed to probe the intricate regulatory mechanism and interaction between transcripts, which was visualized using the Cytoscape software. The ce-lncRNAs of four aberrantly expressed genes, FOSB, EGR3, BCAM and SOX6, were determined through the network. Among the identified DElncRNAs, we selected 8 differentially expressed lncRNAs to confirm the reliability of RNA-seq data by qRT-PCR in 11 KBD patients and 11 healthy individuals. We aimed to provide a comprehensive understanding ofmRNA, lncRNA and miRNA alterations between KBD patients and healthy individuals, and meanwhile reveal several potential causative molecular and signaling pathways involved in KBD.<\/jats:p>\n                  <jats:p\/>","DOI":"10.1039\/d1mo00370d","type":"journal-article","created":{"date-parts":[[2021,12,16]],"date-time":"2021-12-16T07:04:20Z","timestamp":1639638260000},"page":"154-166","update-policy":"https:\/\/doi.org\/10.1039\/rsc_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Comprehensive expression profiles of mRNAs, lncRNAs and miRNAs in Kashin-Beck disease identified by RNA-sequencing"],"prefix":"10.1093","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5529-3179","authenticated-orcid":false,"given":"Yu","family":"Dai","sequence":"first","affiliation":[{"name":"School of Public Health, Xi\u2032an Jiaotong University Health Science Center a , No. 76 Yanta West Road, Xi\u2032an, Shaanxi 710061, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Can","family":"Jian","sequence":"additional","affiliation":[{"name":"School of Public Health, Xi\u2032an Jiaotong University Health Science Center a , No. 76 Yanta West Road, Xi\u2032an, Shaanxi 710061, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofeng","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Public Health, Xi\u2032an Jiaotong University Health Science Center a , No. 76 Yanta West Road, Xi\u2032an, Shaanxi 710061, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6307-0300","authenticated-orcid":false,"given":"Xiaoxia","family":"Dai","sequence":"additional","affiliation":[{"name":"Key Laboratory of Trace Elements and Endemic Diseases, National Health Commission of the People's Republic of China b , No. 76 Yanta West Road, Xi\u2032an, Shaanxi 710061, China \u00a0 xxiadai@mail.xjtu.edu.cn"},{"name":"School of Public Health, Xi\u2032an Jiaotong University Health Science Center a , No. 76 Yanta West Road, Xi\u2032an, Shaanxi 710061, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2021,12,16]]},"reference":[{"key":"2026010813074077000_cit1"},{"issue":"5","key":"2026010813074077000_cit2","first-page":"351","article-title":"Increased Chondrocyte Apoptosis in Kashin-Beck Disease and Rats Induced by T-2 Toxin and Selenium Deficiency","volume":"30","author":"Yang","year":"2017","journal-title":"Biomed. Environ. Sci."},{"issue":"2","key":"2026010813074077000_cit3","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/j.joca.2016.09.019","article-title":"Genetic association, mRNA and protein expression analysis identify ATG4C as a susceptibility gene for Kashin\u2013Beck disease","volume":"25","author":"Wu","year":"2017","journal-title":"Osteoarthr. Cartil."},{"key":"2026010813074077000_cit4","first-page":"151","article-title":"Clinical manifestations of Kashin-Beck disease in Nyemo Valley, Tibet","author":"Mathieu","year":"1997"},{"issue":"9","key":"2026010813074077000_cit5","doi-asserted-by":"crossref","first-page":"578","DOI":"10.1302\/2046-3758.99.BJR-2019-0247.R1","article-title":"The molecular mechanism study of COMP involved in the articular cartilage damage of Kashin-Beck disease","volume":"9","author":"Ma","year":"2020","journal-title":"Bone Jt. Res."},{"key":"2026010813074077000_cit6","first-page":"481","article-title":"Progression and prospect of etiology and pathogenesis of Kashin-Beck disease","author":"Guo","year":"2008","journal-title":"J. Xi'an Jiaotong Univ."},{"issue":"1","key":"2026010813074077000_cit7","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41598-017-17875-0","article-title":"Long noncoding RNA expression profile reveals lncRNAs signature associated with extracellular matrix degradation in Kashin-beck disease","volume":"7","author":"Wu","year":"2017","journal-title":"Sci. Rep."},{"issue":"1","key":"2026010813074077000_cit8","first-page":"1","article-title":"Comparison of microRNA expression profiles of Kashin-Beck disease, osteoarthritis and rheumatoid arthritis","volume":"7","author":"Wu","year":"2017","journal-title":"Sci. Rep."},{"key":"2026010813074077000_cit9","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fcell.2016.00128","article-title":"A lncRNA Perspective into (Re)building the heart","volume":"4","author":"Frank","year":"2016","journal-title":"Front. Cell Dev. Biol."},{"issue":"6","key":"2026010813074077000_cit10","doi-asserted-by":"crossref","first-page":"904","DOI":"10.1016\/j.molcel.2011.08.018","article-title":"Molecular Mechanisms of Long Noncoding RNAs","volume":"43","author":"Wang","year":"2011","journal-title":"Mol. 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Res."},{"key":"2026010813074077000_cit17","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.mce.2016.11.018","article-title":"Small non-coding RNAs-based bone regulation and targeting therapeutic strategies","volume":"456","author":"Yang","year":"2017","journal-title":"Mol. Cell. Endocrinol."},{"issue":"12","key":"2026010813074077000_cit18","doi-asserted-by":"crossref","first-page":"3347","DOI":"10.1111\/jcmm.13237","article-title":"MiR-29b-3p promotes chondrocyte apoptosis and facilitates the occurrence and development of osteoarthritis by targeting PGRN","volume":"21","author":"Chen","year":"2017","journal-title":"J. Cell. Mol. Med."},{"issue":"12","key":"2026010813074077000_cit19","doi-asserted-by":"crossref","first-page":"2881","DOI":"10.1016\/j.bbamcr.2016.09.010","article-title":"MicroRNA-455-3p modulates cartilage development and degeneration through modification of histone H3 acetylation","volume":"1863","author":"Chen","year":"2016","journal-title":"Biochim. Biophys. Acta \u2013 Mol. Cell Res."},{"issue":"9","key":"2026010813074077000_cit20","doi-asserted-by":"crossref","first-page":"1686","DOI":"10.1111\/1756-185X.13358","article-title":"RNA-seq analysis reveals different gene ontologies and pathways in rheumatoid arthritis and Kashin\u2013Beck disease","volume":"21","author":"Yu","year":"2018","journal-title":"Int. J. Rheum. Dis."},{"issue":"4","key":"2026010813074077000_cit21","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nmeth.3317","article-title":"HISAT: a fast spliced aligner with low memory requirements Daehwan HHS Public Access","volume":"12","author":"Kim","year":"2015","journal-title":"Nat. Methods"},{"issue":"5","key":"2026010813074077000_cit22","doi-asserted-by":"crossref","first-page":"806","DOI":"10.1093\/bib\/bbu048","article-title":"LncTar: A tool for predicting the RNA targets of long noncoding RNAs","volume":"16","author":"Li","year":"2014","journal-title":"Brief. 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