{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T20:56:57Z","timestamp":1788555417150,"version":"build-2803163510"},"reference-count":39,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2022,4,11]],"date-time":"2022-04-11T00:00:00Z","timestamp":1649635200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"},{"start":{"date-parts":[[2023,4,11]],"date-time":"2023-04-11T00:00:00Z","timestamp":1681171200000},"content-version":"am","delay-in-days":365,"URL":"http:\/\/rsc.li\/journals-terms-of-use"}],"funder":[{"DOI":"10.13039\/100001818","name":"Research to Prevent Blindness","doi-asserted-by":"publisher","award":["Unrestricted grant to University of Miami"],"award-info":[{"award-number":["Unrestricted grant to University of Miami"]}],"id":[{"id":"10.13039\/100001818","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000053","name":"National Eye Institute","doi-asserted-by":"publisher","award":["EY14801, EY031292"],"award-info":[{"award-number":["EY14801, EY031292"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100001818","name":"Research to Prevent Blindness","doi-asserted-by":"publisher","award":["Unrestricted grant to University of Miami"],"award-info":[{"award-number":["Unrestricted grant to University of Miami"]}],"id":[{"id":"10.13039\/100001818","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000053","name":"National Eye Institute","doi-asserted-by":"publisher","award":["EY14801, EY031292"],"award-info":[{"award-number":["EY14801, EY031292"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["rsc.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2022,4,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Pseudoexfoliation syndrome (PEX) is a systemic disorder that manifests as a fluffy, proteinaceous fibrillar material throughout the body. In the eye, such deposits result in glaucoma (PEXG), due to impeding aqueous humor outflow. Serum lipid alterations and increased lipid peroxidation have been reported in PEX. We report the first ever comprehensive lipid profiling of the aqueous humor (AH) of PEXG. Our untargeted lipidomic analysis of 23 control, 19 primary open angle glaucoma (POAG), 9 PEX, and 14 PEXG AH patients resulted in the identification of 489 lipid species within 26 lipid classes across PEX, PEXG, POAG, and control AH samples. Multiple cholesterol esters (ChEs), phosphatidylcholines (PCs), triglycerides (TGs), and ceramides (Cers) were present in higher concentrations in the PEXG AH than in all other groups. CerG2GNAc1(d34\u2009:\u20091) was enriched in control samples and depleted in both the PEX and PEXG samples. Machine learning prediction with three supervised logistic regression binary classification tasks showed (1) POAG vs. control, with an 86% accuracy, (2) PEXG vs. control, with a 71% accuracy and (3) PEX vs. control, with an 86% accuracy. In conclusion, the analysis showed that the control (mean peak area 13.54 \u00b1 6) had, on average, a higher total lipid content than the PEX, PEXG, and POAG AH samples. Elevations in Apolipoprotein A-I (APOA1) correlated with an increased abundance of PC lipid species in the AH of patients with PEXG. PC (18\u2009:\u20090\/18\u2009:\u20092), PC (36\u2009:\u20092), and PC (34\u2009:\u20091e) are in low concentrations for PEX but are highly concentrated in PEXG, despite both having similar material deposits, suggesting that they are fundamentally different in composition.<\/jats:p>\n                  <jats:p\/>","DOI":"10.1039\/d1mo00495f","type":"journal-article","created":{"date-parts":[[2022,4,11]],"date-time":"2022-04-11T17:01:25Z","timestamp":1649696485000},"page":"387-396","update-policy":"https:\/\/doi.org\/10.1039\/rsc_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Analyses of pseudoexfoliation aqueous humor lipidome"],"prefix":"10.1093","volume":"18","author":[{"given":"Vanessa","family":"Collao","sequence":"first","affiliation":[{"name":"Vision Science and Investigative Ophthalmology Graduate Program, University of Miami c , Miami, Florida, USA"},{"name":"Miami Integrative Metabolomics Research Center, University of Miami b , Miami, USA"},{"name":"Bascom Palmer Eye Institute, University of Miami a , Miami, Florida, USA \u00a0 sbhattacharya@med.miami.edu"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jada","family":"Morris","sequence":"additional","affiliation":[{"name":"Vision Science and Investigative Ophthalmology Graduate Program, University of Miami c , Miami, Florida, USA"},{"name":"Miami Integrative Metabolomics Research Center, University of Miami b , Miami, USA"},{"name":"Bascom Palmer Eye Institute, University of Miami a , Miami, Florida, USA \u00a0 sbhattacharya@med.miami.edu"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Zain","family":"Chauhan","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Jones Eye Institute, University of Arkansas for Medical Sciences Little Rock d , Arkansas, USA"},{"name":"Vision Science and Investigative Ophthalmology Graduate Program, University of Miami c , Miami, Florida, USA"},{"name":"Miami Integrative Metabolomics Research Center, University of Miami b , Miami, USA"},{"name":"Bascom Palmer Eye Institute, University of Miami a , Miami, Florida, USA \u00a0 sbhattacharya@med.miami.edu"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leila","family":"Abdelrahman","sequence":"additional","affiliation":[{"name":"Vision Science and Investigative Ophthalmology Graduate Program, University of Miami c , Miami, Florida, USA"},{"name":"Miami Integrative Metabolomics Research Center, University of Miami b , Miami, USA"},{"name":"Bascom Palmer Eye Institute, University of Miami a , Miami, Florida, USA \u00a0 sbhattacharya@med.miami.edu"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose Mar\u00eda","family":"Mart\u00ednez-de-la-Casa","sequence":"additional","affiliation":[{"name":"Departamento de Inmunolog\u00eda, Oftalmolog\u00eda y ORL, Facultad de Medicina, Universidad Complutense de Madrid f , Madrid, Spain"},{"name":"Servicio de Oftalmolog\u00eda, Hospital Cl\u00ednico San Carlos; IdISSC e , Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beatriz","family":"Vidal-Villegas","sequence":"additional","affiliation":[{"name":"Servicio de Oftalmolog\u00eda, Hospital Cl\u00ednico San Carlos; IdISSC e , Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2178-6164","authenticated-orcid":false,"given":"Barbara","family":"Burgos-Blasco","sequence":"additional","affiliation":[{"name":"Servicio de Oftalmolog\u00eda, Hospital Cl\u00ednico San Carlos; IdISSC e , Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3759-647X","authenticated-orcid":false,"given":"Sanjoy K","family":"Bhattacharya","sequence":"additional","affiliation":[{"name":"Vision Science and Investigative Ophthalmology Graduate Program, University of Miami c , Miami, Florida, USA"},{"name":"Miami Integrative Metabolomics Research Center, University of Miami b , Miami, USA"},{"name":"Bascom Palmer Eye Institute, University of Miami a , Miami, Florida, USA \u00a0 sbhattacharya@med.miami.edu"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,4,11]]},"reference":[{"key":"2026010813074637600_cit1","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1186\/1479-7364-6-22","article-title":"Pseudoexfoliation syndrome, a systemic disorder with ocular manifestations","volume":"6","author":"Eman Elhawy","year":"2012","journal-title":"Human Genomics"},{"key":"2026010813074637600_cit2","doi-asserted-by":"crossref","first-page":"5679","DOI":"10.1167\/iovs.11-7274","article-title":"Anterior segment optical coherence tomography analysis of clinically unilateral pseudoexfoliation syndrome: evidence of bilateral involvement and morphologic factors related to asymmetry","volume":"52","author":"Zheng","year":"2011","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"2026010813074637600_cit3","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1097\/ICU.0b013e3282f49cda","article-title":"The molecular pathophysiology of pseudoexfoliation glaucoma","volume":"19","author":"Lee","year":"2008","journal-title":"Curr. Opin. Ophthalmol."},{"key":"2026010813074637600_cit4","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.preteyeres.2016.12.004","article-title":"Aqueous outflow \u2013 A continuum from trabecular meshwork to episcleral veins","volume":"57","author":"Carreon","year":"2017","journal-title":"Prog. Retin. Eye Res."},{"key":"2026010813074637600_cit5","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1080\/09273948.2018.1545913","article-title":"Monocyte\/HDL Ratio and Lymphocyte\/Monocyte Ratio in Patients with Pseudoexfoliation Syndrome","volume":"28","author":"Mirza","year":"2020","journal-title":"Ocul. Immunol. Inflamm."},{"key":"2026010813074637600_cit6","doi-asserted-by":"crossref","first-page":"1911","DOI":"10.1038\/s41433-019-0506-z","article-title":"Atherogenic indices in pseudoexfoliation syndrome","volume":"33","author":"Mirza","year":"2019","journal-title":"Eye"},{"key":"2026010813074637600_cit7","doi-asserted-by":"crossref","first-page":"5231095","DOI":"10.1155\/2017\/5231095","article-title":"Chosen Vascular Risk Markers in Pseudoexfoliation Syndrome: An Age-Related Disorder","volume":"2017","author":"Lesiewska","year":"2017","journal-title":"J. Ophthalmol."},{"key":"2026010813074637600_cit8","doi-asserted-by":"crossref","first-page":"8","DOI":"10.2298\/VSP150320058J","article-title":"The importance of developing atherosclerosis in pseudoexfoliation glaucoma","volume":"74","author":"Janicijevic","year":"2017","journal-title":"Vojnosanit. Pregl."},{"key":"2026010813074637600_cit9","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1097\/IJG.0000000000000603","article-title":"Changes in the Lipidomic Profile of Aqueous Humor in Open-Angle Glaucoma","volume":"26","author":"Cabrerizo","year":"2017","journal-title":"J. Glaucoma."},{"key":"2026010813074637600_cit10","doi-asserted-by":"crossref","first-page":"3856","DOI":"10.1111\/jcmm.14975","article-title":"Endogenous ocular lipids as potential modulators of intraocular pressure","volume":"24","author":"Edwards","year":"2020","journal-title":"J. Cell. Mol. Med."},{"key":"2026010813074637600_cit11","doi-asserted-by":"crossref","first-page":"954","DOI":"10.1016\/j.tibs.2016.08.010","article-title":"Lipidomics: Techniques, Applications, and Outcomes Related to Biomedical Sciences","volume":"41","author":"Yang","year":"2016","journal-title":"Trends Biochem. Sci."},{"key":"2026010813074637600_cit12","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1080\/02713680590968367","article-title":"Glutathione and lipid peroxide changes in pseudoexfoliation syndrome","volume":"30","author":"Gartaganis","year":"2005","journal-title":"Curr. Eye Res."},{"key":"2026010813074637600_cit13","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1038\/ejhg.2010.144","article-title":"Genome-wide association study with DNA pooling identifies variants at CNTNAP2 associated with pseudoexfoliation syndrome","volume":"19","author":"Krumbiegel","year":"2011","journal-title":"Eur. J. Hum. Genet."},{"key":"2026010813074637600_cit14","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/j.exer.2017.12.006","article-title":"GWAS links variants in neuronal development and actin remodeling related loci with pseudoexfoliation syndrome without glaucoma","volume":"168","author":"Zagajewska","year":"2018","journal-title":"Exp. Eye Res."},{"key":"2026010813074637600_cit15","doi-asserted-by":"crossref","first-page":"4238","DOI":"10.1167\/iovs.14-13991","article-title":"Association of LOXL1 polymorphisms with pseudoexfoliation, glaucoma, intraocular pressure, and systemic diseases in a Greek population. The Thessaloniki eye study","volume":"55","author":"Anastasopoulos","year":"2014","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"2026010813074637600_cit16","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.jprot.2013.12.006","article-title":"Comparative proteomic study in serum of patients with primary open-angle glaucoma and pseudoexfoliation glaucoma","volume":"98","author":"Gonzalez-Iglesias","year":"2014","journal-title":"J. Proteomics"},{"key":"2026010813074637600_cit17","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1039\/C9MO00192A","article-title":"Aqueous humor metabolite profile of pseudoexfoliation glaucoma is distinctive","volume":"16","author":"Myer","year":"2020","journal-title":"Mol. Omics."},{"key":"2026010813074637600_cit18","doi-asserted-by":"crossref","first-page":"108024","DOI":"10.1016\/j.exer.2020.108024","article-title":"Differentiation of soluble aqueous humor metabolites in primary open angle glaucoma and controls","volume":"194","author":"Myer","year":"2020","journal-title":"Exp. 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Eye Res."},{"key":"2026010813074637600_cit22","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1016\/j.biochi.2014.01.020","article-title":"Phospholipid profiles of control and glaucomatous human aqueous humor","volume":"101","author":"Edwards","year":"2014","journal-title":"Biochimie"},{"key":"2026010813074637600_cit23","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1007\/978-1-4939-7407-8_10","article-title":"Shotgun Sphingolipid Analysis of Human Aqueous Humor","volume":"1695","author":"Trzeciecka","year":"2018","journal-title":"Methods Mol. 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Rep."},{"key":"2026010813074637600_cit29","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1001\/jama.2021.0507","article-title":"Association of Rare CYP39A1 Variants With Exfoliation Syndrome Involving the Anterior Chamber of the Eye","volume":"325","author":"Genetics of Exfoliation Syndrome","year":"2021","journal-title":"JAMA"},{"key":"2026010813074637600_cit30","doi-asserted-by":"crossref","first-page":"108","DOI":"10.3109\/13816810.2016.1164193","article-title":"Prostaglandins in the eye: Function, expression, and roles in glaucoma","volume":"38","author":"Doucette","year":"2017","journal-title":"Ophthalmic. Genet."},{"key":"2026010813074637600_cit31","doi-asserted-by":"crossref","first-page":"840","DOI":"10.1016\/j.exer.2009.07.008","article-title":"Red blood cell plasmalogens and docosahexaenoic acid are independently reduced in primary open-angle glaucoma","volume":"89","author":"Acar","year":"2009","journal-title":"Exp. 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Ophthalmol."},{"key":"2026010813074637600_cit38","first-page":"299","article-title":"Protein content in aqueous humor from patients with pseudoexfoliation (PEX) investigated by capillary LC MALDI-TOF\/TOF MS","volume":"3","author":"Hardenborg","year":"2009","journal-title":"Proteomics: Clin. Appl."},{"key":"2026010813074637600_cit39","first-page":"2825","article-title":"Scikit-learn: Machine Learning in Python","volume":"12","author":"Pedregosa","year":"2011","journal-title":"J. Mach. Learn. Res."}],"container-title":["Molecular Omics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/molecular-omics\/article-pdf\/18\/5\/387\/65032550\/d1mo00495f.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/molecular-omics\/article-pdf\/18\/5\/387\/65032550\/d1mo00495f.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T18:10:29Z","timestamp":1767895829000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/molecular-omics\/article\/18\/5\/387\/8286536"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,4,11]]},"references-count":39,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2022,4,11]]}},"URL":"https:\/\/doi.org\/10.1039\/d1mo00495f","relation":{},"ISSN":["2515-4184"],"issn-type":[{"value":"2515-4184","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2022,6,1]]},"published":{"date-parts":[[2022,4,11]]}}}