{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T21:46:45Z","timestamp":1787262405449,"version":"build-2736575974"},"reference-count":47,"publisher":"Royal Society of Chemistry (RSC)","issue":"27","license":[{"start":{"date-parts":[[2021,5,4]],"date-time":"2021-05-04T00:00:00Z","timestamp":1620086400000},"content-version":"vor","delay-in-days":123,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/3.0\/"}],"funder":[{"name":"Technology Agency of the Czech Republic","award":["TJ01000077"],"award-info":[{"award-number":["TJ01000077"]}]},{"name":"Technology Agency of the Czech Republic","award":["UJEP-IGA-TC-2019-53-01-2"],"award-info":[{"award-number":["UJEP-IGA-TC-2019-53-01-2"]}]},{"name":"Technology Agency of the Czech Republic","award":["UJEP-SGS-2018-53-005-3"],"award-info":[{"award-number":["UJEP-SGS-2018-53-005-3"]}]},{"name":"Technology Agency of the Czech Republic","award":["UJEP-SGS-2020-53-006-3"],"award-info":[{"award-number":["UJEP-SGS-2020-53-006-3"]}]},{"DOI":"10.13039\/501100001823","name":"Ministerstvo \u0160kolstv\u00ed, Ml\u00e1de\u017ee a T\u011blov\u00fdchovy","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001823","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"crossref","award":["CZ.02.1.01\/0.0\/0.0\/17_048\/0007411"],"award-info":[{"award-number":["CZ.02.1.01\/0.0\/0.0\/17_048\/0007411"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["rsc.org"],"crossmark-restriction":true},"short-container-title":["RSC Adv."],"abstract":"<p>E-Shell 300 3D-printed material demonstrated a considerable negative impact on cell proliferation and severe developmental toxicity due to release of surfactant residues. Post-treatment with ethanol improved the biocompatibility of the material.<\/p>","DOI":"10.1039\/d1ra00305d","type":"journal-article","created":{"date-parts":[[2021,5,4]],"date-time":"2021-05-04T03:44:22Z","timestamp":1620099862000},"page":"16252-16267","update-policy":"https:\/\/doi.org\/10.1039\/rsc_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Class II biocompatible E-Shell 300 3D printing material causes severe developmental toxicity in\n                    <i>Danio rerio<\/i>\n                    embryos and reduced cell proliferation\n                    <i>in vitro<\/i>\n                    \u2013 implications for 3D printed microfluidics"],"prefix":"10.1039","volume":"11","author":[{"given":"Zuzana","family":"Nejedl\u00e1","sequence":"first","affiliation":[{"name":"Department of Biology"},{"name":"Faculty of Science"},{"name":"Jan Evangelista Purkinje University"},{"name":"Usti nad Labem"},{"name":"Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Poustka","sequence":"additional","affiliation":[{"name":"Department of Biology"},{"name":"Faculty of Science"},{"name":"Jan Evangelista Purkinje University"},{"name":"Usti nad Labem"},{"name":"Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Regina","family":"Herma","sequence":"additional","affiliation":[{"name":"Department of Biology"},{"name":"Faculty of Science"},{"name":"Jan Evangelista Purkinje University"},{"name":"Usti nad Labem"},{"name":"Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michaela","family":"Liegertov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Biology"},{"name":"Faculty of Science"},{"name":"Jan Evangelista Purkinje University"},{"name":"Usti nad Labem"},{"name":"Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3903-6054","authenticated-orcid":false,"given":"Marcel","family":"\u0160tofik","sequence":"additional","affiliation":[{"name":"Department of Biology"},{"name":"Faculty of Science"},{"name":"Jan Evangelista Purkinje University"},{"name":"Usti nad Labem"},{"name":"Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji\u0159\u00ed","family":"Smejkal","sequence":"additional","affiliation":[{"name":"Department of Biology"},{"name":"Faculty of Science"},{"name":"Jan Evangelista Purkinje University"},{"name":"Usti nad Labem"},{"name":"Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V\u00e1clav","family":"\u0160\u00edcha","sequence":"additional","affiliation":[{"name":"Department of Biology"},{"name":"Faculty of Science"},{"name":"Jan Evangelista Purkinje University"},{"name":"Usti nad Labem"},{"name":"Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pavel","family":"Kaule","sequence":"additional","affiliation":[{"name":"Department of Biology"},{"name":"Faculty of Science"},{"name":"Jan Evangelista Purkinje University"},{"name":"Usti nad Labem"},{"name":"Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7022-2157","authenticated-orcid":false,"given":"Jan","family":"Mal\u00fd","sequence":"additional","affiliation":[{"name":"Department of Biology"},{"name":"Faculty of Science"},{"name":"Jan Evangelista Purkinje University"},{"name":"Usti nad Labem"},{"name":"Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"292","published-online":{"date-parts":[[2021]]},"reference":[{"key":"D1RA00305D-(cit1)\/*[position()=1]","doi-asserted-by":"publisher","first-page":"395","DOI":"10.1002\/jbm.10295","volume":"62","author":"Matsuda","year":"2002","journal-title":"J. Biomed. Mater. Res."},{"key":"D1RA00305D-(cit2)\/*[position()=1]","doi-asserted-by":"publisher","first-page":"758","DOI":"10.3357\/ASEM.3898.2014","volume":"85","author":"Wong","year":"2014","journal-title":"Aviat., Space Environ. Med."},{"key":"D1RA00305D-(cit3)\/*[position()=1]","first-page":"203","author":"Boyd","year":"2000","journal-title":"J. Clin. Orthod."},{"key":"D1RA00305D-(cit4)\/*[position()=1]","doi-asserted-by":"publisher","first-page":"230","DOI":"10.1016\/S0735-1097(99)00498-2","volume":"35","author":"Binder","year":"2000","journal-title":"J. Am. Coll. Cardiol."},{"key":"D1RA00305D-(cit6)\/*[position()=1]","doi-asserted-by":"publisher","first-page":"1720","DOI":"10.1039\/C6LC00163G","volume":"16","author":"Bhattacharjee","year":"2016","journal-title":"Lab Chip"},{"key":"D1RA00305D-(cit7)\/*[position()=1]","doi-asserted-by":"publisher","first-page":"22","DOI":"10.1016\/j.mattod.2017.07.001","volume":"21","author":"Tofail","year":"2018","journal-title":"Mater. Today"},{"key":"D1RA00305D-(cit8)\/*[position()=1]","doi-asserted-by":"publisher","first-page":"596","DOI":"10.1016\/j.memsci.2016.10.006","volume":"523","author":"Low","year":"2017","journal-title":"J. Membr. Sci."},{"key":"D1RA00305D-(cit9)\/*[position()=1]","volume-title":"EY's Glob. 3D Print. Rep. 2016","author":"Muller","year":"2016","unstructured":"A.Muller  and  S.Karevska ,  How 3D Printing Technology Could Change World Trade , in  EY's Glob. 3D Print. Rep. 2016 ,  2016 ,  https:\/\/www.ey.com\/Publication\/vwLUAssets\/ey-global-3d-printing-report-2016-fullreport\/$FILE\/ey-global-3d-printing-report-2016-full-report.pdf"},{"key":"D1RA00305D-(cit10)\/*[position()=1]","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1038\/17989","volume":"398","author":"Cumpston","year":"1999","journal-title":"Nature"},{"key":"D1RA00305D-(cit11)\/*[position()=1]","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1089\/zeb.2017.1488","volume":"15","author":"Monstad-Rios","year":"2018","journal-title":"Zebrafish"},{"key":"D1RA00305D-(cit12)\/*[position()=1]","doi-asserted-by":"publisher","first-page":"36","DOI":"10.1186\/1472-6750-14-36","volume":"14","author":"Wittbrodt","year":"2014","journal-title":"BMC Biotechnol."},{"key":"D1RA00305D-(cit13)\/*[position()=1]","doi-asserted-by":"publisher","first-page":"3281","DOI":"10.1002\/jbm.a.36188","volume":"105","author":"Tan","year":"2017","journal-title":"J. Biomed. Mater. Res., Part A"},{"key":"D1RA00305D-(cit14)\/*[position()=1]","doi-asserted-by":"publisher","first-page":"045005","DOI":"10.1088\/1758-5090\/aa8858","volume":"9","author":"Ong","year":"2017","journal-title":"Biofabrication"},{"key":"D1RA00305D-(cit15)\/*[position()=1]","doi-asserted-by":"publisher","first-page":"291","DOI":"10.1039\/C5LC01374G","volume":"16","author":"Macdonald","year":"2016","journal-title":"Lab Chip"},{"key":"D1RA00305D-(cit16)\/*[position()=1]","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1021\/acs.estlett.5b00249","volume":"3","author":"Oskui","year":"2016","journal-title":"Environ. Sci. Technol. Lett."},{"key":"D1RA00305D-(cit17)\/*[position()=1]","doi-asserted-by":"publisher","first-page":"061103","DOI":"10.1063\/1.4939031","volume":"9","author":"Zhu","year":"2015","journal-title":"Biomicrofluidics"},{"key":"D1RA00305D-(cit18)\/*[position()=1]","doi-asserted-by":"publisher","first-page":"153","DOI":"10.1007\/s40846-016-0118-z","volume":"36","author":"Schmelzer","year":"2016","journal-title":"J. Med. Biol. Eng."},{"key":"D1RA00305D-(cit19)\/*[position()=1]","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1023\/B:JMSM.0000011812.08185.2a","volume":"15","author":"Popov","year":"2004","journal-title":"J. Mater. Sci.: Mater. Med."},{"key":"D1RA00305D-(cit20)\/*[position()=1]","doi-asserted-by":"publisher","first-page":"200","DOI":"10.1054\/bjps.1999.3268","volume":"53","author":"D'Urso","year":"2000","journal-title":"Br. J. Plast. Surg."},{"key":"D1RA00305D-(cit21)\/*[position()=1]","doi-asserted-by":"publisher","first-page":"91","DOI":"10.3390\/mi9020091","volume":"9","author":"Carve","year":"2018","journal-title":"Micromachines"},{"key":"D1RA00305D-(cit22)\/*[position()=1]","doi-asserted-by":"publisher","first-page":"046502","DOI":"10.1063\/1.4927379","volume":"9","author":"Zhu","year":"2015","journal-title":"Biomicrofluidics"},{"key":"D1RA00305D-(cit23)\/*[position()=1]","doi-asserted-by":"publisher","first-page":"3309","DOI":"10.1016\/j.scitotenv.2011.04.038","volume":"409","author":"Lithner","year":"2011","journal-title":"Sci. Total Environ."},{"key":"D1RA00305D-(cit24)\/*[position()=1]","doi-asserted-by":"publisher","first-page":"333","DOI":"10.1177\/10454411000110030401","volume":"11","author":"Geurtsen","year":"2000","journal-title":"Crit. Rev. Oral Biol. Med."},{"key":"D1RA00305D-(cit25)\/*[position()=1]","doi-asserted-by":"publisher","first-page":"290","DOI":"10.1016\/0109-5641(92)90102-I","volume":"8","author":"Ferracane","year":"1992","journal-title":"Dent. Mater."},{"key":"D1RA00305D-(cit26)\/*[position()=1]","doi-asserted-by":"publisher","first-page":"80","DOI":"10.1021\/acs.estlett.7b00495","volume":"5","author":"de Almeida Monteiro Melo Ferraz","year":"2018","journal-title":"Environ. Sci. Technol. Lett."},{"key":"D1RA00305D-(cit27)\/*[position()=1]","doi-asserted-by":"publisher","first-page":"115","DOI":"10.1016\/j.procir.2013.01.023","volume":"5","author":"Inoue","year":"2013","journal-title":"Procedia CIRP"},{"key":"D1RA00305D-(cit30)\/*[position()=1]","volume-title":"Biocompatibility of dental materials","author":"Schmalz","year":"2009","unstructured":"G.Schmalz  and  D.Arenholt-Bindslev ,  Biocompatibility of dental materials ,  Springer ,  Berlin ,  2009"},{"key":"D1RA00305D-(cit31)\/*[position()=1]","doi-asserted-by":"publisher","first-page":"71","DOI":"10.3390\/mi9020071","volume":"9","author":"van den Driesche","year":"2018","journal-title":"Micromachines"},{"key":"D1RA00305D-(cit33)\/*[position()=1]","doi-asserted-by":"publisher","DOI":"10.1787\/9789264203709-en","volume-title":"Test No. 236: Fish Embryo Acute Toxicity\n(FET) Test","author":"OECD","year":"2013","unstructured":"OECD ,  Test No. 236: Fish Embryo Acute Toxicity\n(FET) Test ,  OECD ,  2013 ,  10.1787\/9789264203709-en"},{"issue":"suppl 1","key":"D1RA00305D-(cit34)\/*[position()=1]","first-page":"38","volume":"19","author":"Nagel","year":"2002","journal-title":"ALTEX"},{"key":"D1RA00305D-(cit35)\/*[position()=1]","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-813602-7.00037-5","volume-title":"Introd. Interdiscip. Toxicol.","author":"Padilla","year":"2020","unstructured":"S.Padilla  and  S.Glaberman ,  The zebrafish (Danio rerio) model in toxicity testing , in  Introd. Interdiscip. Toxicol. ,  Elsevier ,  2020 ,  pp. 525\u2013532 ,  10.1016\/B978-0-12-813602-7.00037-5"},{"key":"D1RA00305D-(cit36)\/*[position()=1]","doi-asserted-by":"publisher","first-page":"677","DOI":"10.1016\/j.chemosphere.2017.08.047","volume":"186","author":"Dang","year":"2017","journal-title":"Chemosphere"},{"issue":"9","key":"D1RA00305D-(cit37)\/*[position()=1]","doi-asserted-by":"publisher","DOI":"10.1002\/0471141755.ph0904s44","volume":"44","author":"Entzeroth","year":"2009","journal-title":"Curr. Protoc. Pharmacol."},{"issue":"10","key":"D1RA00305D-(cit38)\/*[position()=1]","doi-asserted-by":"publisher","DOI":"10.1101\/pdb.rec066449","volume":"2011","author":"Sever","year":"2011","journal-title":"Cold Spring Harb. Protoc."},{"issue":"1","key":"D1RA00305D-(cit40)\/*[position()=1]","doi-asserted-by":"publisher","first-page":"175","DOI":"10.1210\/endo.135.1.8013351","volume":"135","author":"White","year":"1994","journal-title":"Endocrinology"},{"key":"D1RA00305D-(cit42)\/*[position()=1]","doi-asserted-by":"publisher","first-page":"431d","DOI":"10.1136\/oem.57.6.431d","volume":"57","author":"Dayan","year":"2000","journal-title":"Occup. Environ. Med."},{"key":"D1RA00305D-(cit43)\/*[position()=1]","doi-asserted-by":"publisher","first-page":"1763","DOI":"10.1007\/s11356-011-0663-5","volume":"19","author":"Lithner","year":"2012","journal-title":"Environ. Sci. Pollut. Res."},{"key":"D1RA00305D-(cit44)\/*[position()=1]","doi-asserted-by":"publisher","first-page":"114","DOI":"10.1016\/j.chemosphere.2015.03.010","volume":"132","author":"Bejgarn","year":"2015","journal-title":"Chemosphere"},{"key":"D1RA00305D-(cit45)\/*[position()=1]","volume-title":"Med. Device Diagn. Ind.","author":"Michaels","year":"2014","unstructured":"B.Michaels ,  A New Biocompatibility Test: Zebrafish Embryo Toxicity Testing ,  Med. Device Diagn. Ind. ,  2014 ,  https:\/\/www.mddionline.com\/new-biocompatibility-test-zebrafish-embryo-toxicity-testing"},{"key":"D1RA00305D-(cit46)\/*[position()=1]","doi-asserted-by":"publisher","first-page":"82","DOI":"10.1016\/j.reprotox.2015.04.001","volume":"53","author":"Lutte","year":"2015","journal-title":"Reprod. Toxicol."},{"key":"D1RA00305D-(cit47)\/*[position()=1]","doi-asserted-by":"publisher","first-page":"769","DOI":"10.1016\/j.ntt.2004.06.012","volume":"26","author":"Reimers","year":"2004","journal-title":"Neurotoxicol. Teratol."},{"issue":"31","key":"D1RA00305D-(cit48)\/*[position()=1]","first-page":"1","volume":"2007","author":"Toyokawa","year":"2007","journal-title":"Kyushu Kyoritsu Daigaku Kogakubu Kenkyu Hokoku"},{"issue":"9","key":"D1RA00305D-(cit49)\/*[position()=1]","doi-asserted-by":"publisher","first-page":"11224","DOI":"10.1007\/s11356-020-11362-2","volume":"28","author":"Jho","year":"2021","journal-title":"Environ. Sci. Pollut. Res."},{"issue":"1","key":"D1RA00305D-(cit50)\/*[position()=1]","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1016\/j.aca.2008.04.043","volume":"627","author":"Keller","year":"2008","journal-title":"Anal. Chim. Acta"},{"key":"D1RA00305D-(cit52)\/*[position()=1]","volume-title":"Biological evaluation of medical devices \u2014 Part 18: Chemical characterization of medical device materials within a risk management process","author":"International Organization for Standardization","year":"2020","unstructured":"International Organization for Standardization ,  Biological evaluation of medical devices \u2014 Part 18: Chemical characterization of medical device materials within a risk management process ,  2020 ,  https:\/\/www.iso.org\/standard\/64750.html"},{"key":"D1RA00305D-(cit54)\/*[position()=1]","doi-asserted-by":"publisher","first-page":"035007","DOI":"10.1088\/1748-605X\/ab09c4","volume":"14","author":"Ngan","year":"2019","journal-title":"Biomed. Mater."},{"key":"D1RA00305D-(cit55)\/*[position()=1]","doi-asserted-by":"publisher","first-page":"52","DOI":"10.3390\/inventions3030052","volume":"3","author":"Alifui-Segbaya","year":"2018","journal-title":"Inventions"}],"container-title":["RSC Advances"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/pubs.rsc.org\/en\/content\/articlepdf\/2021\/RA\/D1RA00305D","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T18:25:40Z","timestamp":1782930340000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.rsc.org\/ra\/article\/11\/27\/16252-16267\/741827"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021]]},"references-count":47,"journal-issue":{"issue":"27","published-print":{"date-parts":[[2021,5,4]]}},"URL":"https:\/\/doi.org\/10.1039\/d1ra00305d","relation":{},"ISSN":["2046-2069"],"issn-type":[{"value":"2046-2069","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021]]},"assertion":[{"value":"This document is Similarity Check deposited","name":"similarity_check","explanation":{"URL":"https:\/\/www.crossref.org\/services\/similarity-check\/"},"group":{"name":"similarity_check","label":"SIMILARITY CHECK"}},{"value":"Marcel \u0160tofik (ORCID)","URL":"http:\/\/orcid.org\/0000-0003-3903-6054","name":"identifier","group":{"name":"author_identifiers","label":"IDENTIFIERS"}},{"value":"Jan Mal\u00fd (ORCID)","URL":"http:\/\/orcid.org\/0000-0002-7022-2157","name":"identifier","group":{"name":"author_identifiers","label":"IDENTIFIERS"}},{"value":"Jan Mal\u00fd (ResearcherID)","URL":"http:\/\/www.researcherid.com\/rid\/B-6434-2011","name":"identifier","group":{"name":"author_identifiers","label":"IDENTIFIERS"}},{"value":"Single-blind","order":2,"name":"peer_review_method","group":{"name":"peer_review_method","label":"PEER REVIEW METHOD"}},{"value":"Received 13 January 2021; Accepted 8 April 2021;  Version of Record published 4 May 2021","order":1,"name":"history","group":{"name":"publication_history","label":"PUBLICATION HISTORY"}}]}}