{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T16:14:00Z","timestamp":1790093640749,"version":"4.0.1"},"reference-count":39,"publisher":"Royal Society of Chemistry (RSC)","issue":"8","license":[{"start":{"date-parts":[[2022,2,10]],"date-time":"2022-02-10T00:00:00Z","timestamp":1644451200000},"content-version":"vor","delay-in-days":40,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/3.0\/"}],"funder":[{"DOI":"10.13039\/100010661","name":"Horizon 2020 Framework Programme","doi-asserted-by":"publisher","award":["675671; 861194"],"award-info":[{"award-number":["675671; 861194"]}],"id":[{"id":"10.13039\/100010661","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["rsc.org"],"crossmark-restriction":true},"short-container-title":["Chem. Sci."],"abstract":"<jats:p>The introduction of glycoconjugate vaccines marks an important point in the fight against various infectious diseases.<\/jats:p>","DOI":"10.1039\/d1sc01928g","type":"journal-article","created":{"date-parts":[[2022,2,10]],"date-time":"2022-02-10T04:22:25Z","timestamp":1644466945000},"page":"2440-2449","update-policy":"https:\/\/doi.org\/10.1039\/rsc_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Retaining the structural integrity of disulfide bonds in diphtheria toxoid carrier protein is crucial for the effectiveness of glycoconjugate vaccine candidates"],"prefix":"10.1039","volume":"13","author":[{"given":"Filippo","family":"Carboni","sequence":"first","affiliation":[{"name":"GSK, Via Fiorentina 10, 53100 Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8001-5928","authenticated-orcid":false,"given":"Annabel","family":"Kitowski","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Molecular, Faculdade de Medicina da Universidade de Lisboa, Lisboa, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1999-6007","authenticated-orcid":false,"given":"Charlotte","family":"Sorieul","sequence":"additional","affiliation":[{"name":"GSK, Via Fiorentina 10, 53100 Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniele","family":"Veggi","sequence":"additional","affiliation":[{"name":"GSK, Via Fiorentina 10, 53100 Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta C.","family":"Marques","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Molecular, Faculdade de Medicina da Universidade de Lisboa, Lisboa, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Davide","family":"Oldrini","sequence":"additional","affiliation":[{"name":"GSK, Via Fiorentina 10, 53100 Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evita","family":"Balducci","sequence":"additional","affiliation":[{"name":"GSK, Via Fiorentina 10, 53100 Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Brogioni","sequence":"additional","affiliation":[{"name":"GSK, Via Fiorentina 10, 53100 Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda","family":"Del Bino","sequence":"additional","affiliation":[{"name":"GSK, Via Fiorentina 10, 53100 Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessio","family":"Corrado","sequence":"additional","affiliation":[{"name":"GSK, Via Fiorentina 10, 53100 Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Angiolini","sequence":"additional","affiliation":[{"name":"GSK, Via Fiorentina 10, 53100 Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucia","family":"Dello Iacono","sequence":"additional","affiliation":[{"name":"GSK, Via Fiorentina 10, 53100 Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Immaculada","family":"Margarit","sequence":"additional","affiliation":[{"name":"GSK, Via Fiorentina 10, 53100 Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Rosaria","family":"Romano","sequence":"additional","affiliation":[{"name":"GSK, Via Fiorentina 10, 53100 Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6594-8917","authenticated-orcid":false,"given":"Gon\u00e7alo J. L.","family":"Bernardes","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Molecular, Faculdade de Medicina da Universidade de Lisboa, Lisboa, Portugal"},{"name":"Yusuf Hamied Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5228-6088","authenticated-orcid":false,"given":"Roberto","family":"Adamo","sequence":"additional","affiliation":[{"name":"GSK, Via Fiorentina 10, 53100 Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"292","published-online":{"date-parts":[[2022]]},"reference":[{"key":"D1SC01928G\/cit1\/1","doi-asserted-by":"crossref","first-page":"1048","DOI":"10.3389\/fimmu.2020.01048","volume":"11","author":"Rosini","year":"2020","journal-title":"Front. Immunol."},{"key":"D1SC01928G\/cit2\/1","doi-asserted-by":"crossref","first-page":"eaat4615","DOI":"10.1126\/scitranslmed.aat4615","volume":"9","author":"Rappuoli","year":"2018","journal-title":"Sci. Transl. Med."},{"key":"D1SC01928G\/cit3\/1","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1073\/pnas.1819612116","volume":"116","author":"Rappuoli","year":"2019","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"D1SC01928G\/cit4\/1","doi-asserted-by":"crossref","first-page":"9015","DOI":"10.1039\/C8CS00495A","volume":"47","author":"Berti","year":"2018","journal-title":"Chem. Soc. Rev."},{"key":"D1SC01928G\/cit5\/1","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1038\/nchem.2415","volume":"8","author":"Chudasama","year":"2016","journal-title":"Nat. Chem."},{"key":"D1SC01928G\/cit6\/1","doi-asserted-by":"crossref","first-page":"1270","DOI":"10.1021\/acs.accounts.7b00106","volume":"50","author":"Adamo","year":"2017","journal-title":"Acc. Chem. Res."},{"key":"D1SC01928G\/cit7\/1","doi-asserted-by":"crossref","first-page":"2174","DOI":"10.1021\/cr500399p","volume":"115","author":"Boutureira","year":"2015","journal-title":"Chem. Rev."},{"key":"D1SC01928G\/cit8\/1","first-page":"13198","volume":"54","author":"Bernardes","year":"2006","journal-title":"Angew. Chem., Int. Ed. Engl."},{"issue":"726","key":"D1SC01928G\/cit9\/1","doi-asserted-by":"publisher","DOI":"10.3389\/fchem.2019.00726","volume":"7","author":"Pillot","year":"2019","journal-title":"Front. Chem."},{"key":"D1SC01928G\/cit10\/1","doi-asserted-by":"crossref","first-page":"13198","DOI":"10.1002\/anie.201506112","volume":"54","author":"Stefanetti","year":"2015","journal-title":"Angew. Chem., Int. Ed. Engl."},{"key":"D1SC01928G\/cit11\/1","doi-asserted-by":"crossref","first-page":"6483","DOI":"10.1002\/chem.201700745","volume":"23","author":"Boutureira","year":"2017","journal-title":"Chem. Eur. J."},{"key":"D1SC01928G\/cit12\/1","doi-asserted-by":"crossref","first-page":"2105","DOI":"10.1021\/bc500438h","volume":"25","author":"Nilo","year":"2014","journal-title":"Bioconjugate Chem."},{"key":"D1SC01928G\/cit13\/1","doi-asserted-by":"crossref","first-page":"1839","DOI":"10.1021\/acs.bioconjchem.5b00365","volume":"26","author":"Nilo","year":"2015","journal-title":"Bioconjugate Chem."},{"key":"D1SC01928G\/cit14\/1","doi-asserted-by":"crossref","first-page":"3827","DOI":"10.1039\/c3sc51694f","volume":"4","author":"Hu","year":"2013","journal-title":"Chem. Sci."},{"key":"D1SC01928G\/cit15\/1","doi-asserted-by":"crossref","first-page":"2995","DOI":"10.1039\/c3sc50862e","volume":"4","author":"Adamo","year":"2013","journal-title":"Chem. Sci."},{"key":"D1SC01928G\/cit16\/1","doi-asserted-by":"crossref","first-page":"4302","DOI":"10.1039\/C4SC01361A","volume":"5","author":"Adamo","year":"2014","journal-title":"Chem. Sci."},{"key":"D1SC01928G\/cit17\/1","doi-asserted-by":"crossref","first-page":"1737","DOI":"10.1021\/acschembio.5b00247","volume":"10","author":"Nilo","year":"2015","journal-title":"ACS Chem. Biol."},{"issue":"44","key":"D1SC01928G\/cit18\/1","doi-asserted-by":"crossref","first-page":"9018","DOI":"10.1039\/D0OB01912G","volume":"18","author":"Szijj","year":"2020","journal-title":"Org. Biomol. Chem."},{"key":"D1SC01928G\/cit19\/1","doi-asserted-by":"crossref","first-page":"3435","DOI":"10.1016\/j.vaccine.2012.03.023","volume":"30","author":"Garufi","year":"2012","journal-title":"Vaccine"},{"key":"D1SC01928G\/cit20\/1","doi-asserted-by":"crossref","first-page":"1567","DOI":"10.1021\/ja906611x","volume":"132","author":"Wu","year":"2010","journal-title":"J. Am. Chem. Soc."},{"key":"D1SC01928G\/cit21\/1","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1021\/acs.biochem.7b01099","volume":"57","author":"Kapoor","year":"2018","journal-title":"Biochemistry"},{"key":"D1SC01928G\/cit22\/1","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1038\/s41570-019-0079-1","volume":"3","author":"Hoyt","year":"2019","journal-title":"Nat. Rev. Chem."},{"key":"D1SC01928G\/cit23\/1","doi-asserted-by":"crossref","first-page":"1666","DOI":"10.1039\/c1sc00185j","volume":"2","author":"Chalker","year":"2011","journal-title":"Chem. Sci."},{"key":"D1SC01928G\/cit24\/1","doi-asserted-by":"crossref","first-page":"630","DOI":"10.1002\/asia.200800427","volume":"4","author":"Chalker","year":"2009","journal-title":"Chem.\u2013Asian J."},{"key":"D1SC01928G\/cit25\/1","first-page":"941","volume":"51","author":"Bernardes","year":"2012","journal-title":"Chem. Sci."},{"key":"D1SC01928G\/cit26\/1","first-page":"123","volume-title":"Methods in Enzymology","volume":"502","author":"Lyon","year":"2012","unstructured":"R. P.Lyon ,  D. L.Meyer ,  J. R.Setter  and  P. D.Senter , in  Methods in Enzymology , ed.  K. D. Wittrup  and  G. L. Verdine ,  Academic Press ,  2012 ,  vol. 502 ,  pp. 123\u2013138"},{"key":"D1SC01928G\/cit27\/1","doi-asserted-by":"crossref","first-page":"5229","DOI":"10.1073\/pnas.1201964109","volume":"109","author":"Malito","year":"2012","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"D1SC01928G\/cit28\/1","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/j.biologicals.2011.05.004","volume":"39","author":"Br\u00f6ker","year":"2011","journal-title":"Biologicals"},{"key":"D1SC01928G\/cit29\/1","doi-asserted-by":"crossref","first-page":"2505","DOI":"10.4161\/hv.26109","volume":"9","author":"Pichichero","year":"2013","journal-title":"Hum. Vaccines Immunother."},{"issue":"47","key":"D1SC01928G\/cit30\/1","doi-asserted-by":"crossref","first-page":"14963","DOI":"10.1002\/anie.201708847","volume":"56","author":"Mart\u00ednez-S\u00e1ez","year":"2017","journal-title":"Angew. Chem., Int. Ed."},{"key":"D1SC01928G\/cit31\/1","doi-asserted-by":"crossref","first-page":"W315","DOI":"10.1093\/nar\/gky497","volume":"46","author":"Micsonai","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"D1SC01928G\/cit32\/1","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1038\/357216a0","volume":"357","author":"Choe","year":"1992","journal-title":"Nature"},{"key":"D1SC01928G\/cit33\/1","doi-asserted-by":"crossref","first-page":"5017","DOI":"10.1073\/pnas.1701885114","volume":"114","author":"Carboni","year":"2017","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"D1SC01928G\/cit34\/1","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/S1473-3099(20)30478-3","volume":"21","author":"Absalon","year":"2020","journal-title":"Lancet Infect. Dis."},{"key":"D1SC01928G\/cit35\/1","doi-asserted-by":"crossref","first-page":"923","DOI":"10.1016\/S1473-3099(16)00152-3","volume":"16","author":"Madhi","year":"2016","journal-title":"Lancet Infect. Dis."},{"key":"D1SC01928G\/cit36\/1","doi-asserted-by":"crossref","first-page":"836","DOI":"10.1002\/cbic.201300785","volume":"15","author":"Crotti","year":"2014","journal-title":"ChemBioChem"},{"key":"D1SC01928G\/cit37\/1","doi-asserted-by":"crossref","first-page":"e010776","DOI":"10.1136\/bmjopen-2015-010776","volume":"6","author":"Delgleize","year":"2016","journal-title":"BMJ Open"},{"issue":"2","key":"D1SC01928G\/cit38\/1","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1016\/j.vaccine.2014.11.026","volume":"33","author":"Pecetta","year":"2015","journal-title":"Vaccine"},{"issue":"20","key":"D1SC01928G\/cit39\/1","doi-asserted-by":"crossref","first-page":"2334","DOI":"10.1016\/j.vaccine.2016.03.055","volume":"34","author":"Pecetta","year":"2016","journal-title":"Vaccine"}],"container-title":["Chemical Science"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/pubs.rsc.org\/en\/content\/articlepdf\/2022\/SC\/D1SC01928G","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T02:59:15Z","timestamp":1782874755000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.rsc.org\/sc\/article\/13\/8\/2440-2449\/746148"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022]]},"references-count":39,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2022,2,23]]}},"URL":"https:\/\/doi.org\/10.1039\/d1sc01928g","relation":{},"ISSN":["2041-6520","2041-6539"],"issn-type":[{"value":"2041-6520","type":"print"},{"value":"2041-6539","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022]]}}}