{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T23:21:53Z","timestamp":1767914513564,"version":"3.49.0"},"reference-count":34,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2023,4,26]],"date-time":"2023-04-26T00:00:00Z","timestamp":1682467200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"},{"start":{"date-parts":[[2024,4,26]],"date-time":"2024-04-26T00:00:00Z","timestamp":1714089600000},"content-version":"am","delay-in-days":366,"URL":"http:\/\/rsc.li\/journals-terms-of-use"}],"funder":[{"DOI":"10.13039\/501100017596","name":"Natural Science Basic Research Program of Shaanxi Province","doi-asserted-by":"publisher","award":["2021JM-037"],"award-info":[{"award-number":["2021JM-037"]}],"id":[{"id":"10.13039\/501100017596","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81620108026"],"award-info":[{"award-number":["81620108026"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017596","name":"Natural Science Basic Research Program of Shaanxi Province","doi-asserted-by":"publisher","award":["2021JM-037"],"award-info":[{"award-number":["2021JM-037"]}],"id":[{"id":"10.13039\/501100017596","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81620108026"],"award-info":[{"award-number":["81620108026"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["rsc.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2023,4,26]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>We aimed to compare N-glycosylation proteins in Kashin\u2013Beck disease (KBD) chondrocytes and normal chondrocytes derived from induced pluripotent stem cells (iPSCs). KBD and normal iPSCs were reprogrammed from human KBD and normal dermal fibroblasts, respectively. Subsequently, chondrocytes were differentiated from KBD and normal iPSCs separately. Immunofluorescence was utilized to assay the protein markers of iPSCs and chondrocytes. Differential N-glycosylation proteins were screened using label-free strategies with LC-MS\/MS. Bioinformatics analyses were utilized to interpret the functions of differential N-glycosylation proteins. Immunofluorescence staining revealed that both KBD-iPSCs and normal-iPSCs strongly expressed pluripotency markers OCT4 and NANOG. Meanwhile, chondrocyte markers collagen II and SOX9 are presented in KBD-iPSC-chondrocytes and normal-iPSC-chondrocytes. We obtained 87 differential N-glycosylation sites which corresponded to 68 differential proteins, which were constructed into 1 cluster. We obtained collagen type I trimer and 9 other biological processes; polysaccharide binding and 9 other molecular functions; regulation of transcription by RNA polymerase II and 9 other cellular components from GO; the Pl3K-Akt signaling pathway and 9 other KEGG pathways; peroxisome and 7 other subcellular locations; and integrin alpha chain, C-terminal cytoplasmic region, conserved site and 9 other classifications of domain annotations, and 2 networks. FGFR3 and LRP1 are expressed at higher levels in KBD-iPSC-chondrocytes, while the expressions of COL2A1, TIMP1, UNC5B, NOG, LEPR, and ITGA1 were down-regulated in KBD-iPSC-chondrocytes. The differential expressions of these N-glycosylation proteins may lead to the abnormal function of KBD chondrocytes.<\/jats:p>\n                  <jats:p\/>","DOI":"10.1039\/d3mo00018d","type":"journal-article","created":{"date-parts":[[2023,4,26]],"date-time":"2023-04-26T02:48:17Z","timestamp":1682477297000},"page":"454-463","update-policy":"https:\/\/doi.org\/10.1039\/rsc_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Analysis of\n                    <i>N<\/i>\n                    -glycosylation protein of Kashin\u2013Beck disease chondrocytes derived from induced pluripotent stem cells based on label-free strategies with LC-MS\/MS"],"prefix":"10.1093","volume":"19","author":[{"given":"Sen","family":"Wang","sequence":"first","affiliation":[{"name":"School of Public Health, Health Science Center, Xi'an Jiaotong University, Key Laboratory of Trace Elements and Endemic Diseases, National Health Commission a , Xi'an, Shaanxi, China \u00a0 guox@xjtu.edu.cn"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peilin","family":"Meng","sequence":"additional","affiliation":[{"name":"School of Public Health, Health Science Center, Xi'an Jiaotong University, Key Laboratory of Trace Elements and Endemic Diseases, National Health Commission a , Xi'an, Shaanxi, China \u00a0 guox@xjtu.edu.cn"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linlin","family":"Yuan","sequence":"additional","affiliation":[{"name":"School of Public Health, Health Science Center, Xi'an Jiaotong University, Key Laboratory of Trace Elements and Endemic Diseases, National Health Commission a , Xi'an, Shaanxi, China \u00a0 guox@xjtu.edu.cn"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8429-8235","authenticated-orcid":false,"given":"Xiong","family":"Guo","sequence":"additional","affiliation":[{"name":"School of Public Health, Health Science Center, Xi'an Jiaotong University, Key Laboratory of Trace Elements and Endemic Diseases, National Health Commission a , Xi'an, Shaanxi, China \u00a0 guox@xjtu.edu.cn"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2023,4,26]]},"reference":[{"issue":"3","key":"2026010813052254000_cit1","first-page":"563","article-title":"Geological environment characteristics and etiology research on Kaschin-Beck disease in China","volume":"37","author":"An","year":"2010","journal-title":"China Geol."},{"issue":"2","key":"2026010813052254000_cit2","first-page":"150","article-title":"Evaluation of the Sensitivity and Specificity of the New Clinical Diagnostic and Classification Criteria for Kashin-Beck Disease, an Endemic Osteoarthritis, in China","volume":"30","author":"Yu","year":"2017","journal-title":"Biomed. Environ. Sci."},{"issue":"1","key":"2026010813052254000_cit3","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1111\/febs.16143","article-title":"The first human induced pluripotent stem cell line of Kashin-Beck disease reveals involvement of heparan sulfate proteoglycan biosynthesis and PPAR pathway","volume":"289","author":"Liu","year":"2022","journal-title":"FEBS J."},{"issue":"5512","key":"2026010813052254000_cit4","doi-asserted-by":"crossref","first-page":"2364","DOI":"10.1126\/science.291.5512.2364","article-title":"Intracellular functions of N-linked glycans","volume":"291","author":"Helenius","year":"2001","journal-title":"Science"},{"issue":"5","key":"2026010813052254000_cit5","doi-asserted-by":"crossref","first-page":"855","DOI":"10.1016\/j.cell.2006.08.019","article-title":"Glycosylation in cellular mechanisms of health and disease","volume":"126","author":"Ohtsubo","year":"2006","journal-title":"Cell"},{"issue":"3","key":"2026010813052254000_cit6","doi-asserted-by":"crossref","first-page":"e12765","DOI":"10.1111\/jpi.12765","article-title":"Melatonin reduces proliferation and promotes apoptosis of bladder cancer cells by suppressing O-GlcNAcylation of cyclin-dependent-like kinase 5","volume":"71","author":"Wu","year":"2021","journal-title":"J. Pineal Res."},{"issue":"5","key":"2026010813052254000_cit7","doi-asserted-by":"crossref","first-page":"855","DOI":"10.1016\/j.cell.2006.08.019","article-title":"Glycosylation in cellular mechanisms of health and disease","volume":"126","author":"Ohtsubo","year":"2006","journal-title":"Cell"},{"issue":"6","key":"2026010813052254000_cit8","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1007\/s10719-020-09958-4","article-title":"Analysis of serum glycome by lectin microarrays for prostate cancer patients - a search for aberrant glycoforms","volume":"37","author":"Bertok","year":"2020","journal-title":"Glycoconjugate J."},{"issue":"1","key":"2026010813052254000_cit9","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1002\/mas.20221","article-title":"Glycoproteomics in neurodegenerative diseases","volume":"29","author":"Hwang","year":"2010","journal-title":"Mass Spectrom. Rev."},{"issue":"4","key":"2026010813052254000_cit10","doi-asserted-by":"crossref","first-page":"1028","DOI":"10.3906\/sag-1903-137","article-title":"Roles of glycoprotein glycosylation in the pathogenesis of an endemic osteoarthritis, Kashin-Beck disease, and effectiveness evaluation of sodium hyaluronate treatment","volume":"50","author":"Wang","year":"2020","journal-title":"Turk. J. Med. Sci."},{"issue":"1","key":"2026010813052254000_cit11","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1111\/febs.16143","article-title":"The first human induced pluripotent stem cell line of Kashin-Beck disease reveals involvement of heparan sulfate proteoglycan biosynthesis and PPAR pathway","volume":"289","author":"Liu","year":"2022","journal-title":"FEBS J."},{"issue":"12","key":"2026010813052254000_cit12","doi-asserted-by":"crossref","first-page":"934","DOI":"10.1007\/s13238-012-2107-5","article-title":"Compound screening platform using human induced pluripotent stem cells to identify small molecules that promote chondrogenesis","volume":"3","author":"Yang","year":"2012","journal-title":"Protein Cell"},{"issue":"6","key":"2026010813052254000_cit13","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1038\/nprot.2015.052","article-title":"NetworkAnalyst for statistical, visual and network-based meta-analysis of gene expression data","volume":"10","author":"Xia","year":"2015","journal-title":"Nat. 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Genet."},{"issue":"16","key":"2026010813052254000_cit16","doi-asserted-by":"crossref","first-page":"7111","DOI":"10.7150\/thno.45286","article-title":"Fgfr3 mutation disrupts chondrogenesis and bone ossification in zebrafish model mimicking CATSHL syndrome partially via enhanced Wnt\/beta-catenin signaling","volume":"10","author":"Sun","year":"2020","journal-title":"Theranostics"},{"issue":"6","key":"2026010813052254000_cit17","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1172\/JCI200113992","article-title":"LRP: a multifunctional scavenger and signaling receptor","volume":"108","author":"Herz","year":"2001","journal-title":"J. Clin. Invest."},{"issue":"2","key":"2026010813052254000_cit18","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1016\/j.bbrc.2006.04.109","article-title":"Possible role of LRP1, a CCN2 receptor, in chondrocytes","volume":"345","author":"Kawata","year":"2006","journal-title":"Biochem. Biophys. Res. Commun."},{"issue":"3","key":"2026010813052254000_cit19","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1002\/jcp.10371","article-title":"TNFalpha suppresses link protein and type II collagen expression in chondrocytes: Role of MEK1\/2 and NF-kappaB signaling pathways","volume":"197","author":"Seguin","year":"2003","journal-title":"J. Cell. Physiol."},{"issue":"1","key":"2026010813052254000_cit20","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.bbapap.2011.06.020","article-title":"Proteases involved in cartilage matrix degradation in osteoarthritis","volume":"1824","author":"Troeberg","year":"2012","journal-title":"Biochim. Biophys. Acta"},{"issue":"25","key":"2026010813052254000_cit21","doi-asserted-by":"crossref","first-page":"18294","DOI":"10.1074\/jbc.M701523200","article-title":"Proteolytic activities of human ADAMTS-5: comparative studies with ADAMTS-4","volume":"282","author":"Gendron","year":"2007","journal-title":"J. Biol. Chem."},{"issue":"10","key":"2026010813052254000_cit22","doi-asserted-by":"crossref","first-page":"6462","DOI":"10.1074\/jbc.M113.545376","article-title":"Low density lipoprotein receptor-related protein 1 (LRP1)-mediated endocytic clearance of a disintegrin and metalloproteinase with thrombospondin motifs-4 (ADAMTS-4): functional differences of non-catalytic domains of ADAMTS-4 and ADAMTS-5 in LRP1 binding","volume":"289","author":"Yamamoto","year":"2014","journal-title":"J. Biol. 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Res."},{"key":"2026010813052254000_cit28","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/j.bbrc.2021.07.044","article-title":"Noggin, an inhibitor of bone morphogenetic protein signaling, antagonizes TGF-beta1 in a mouse model of osteoarthritis","volume":"570","author":"Wen","year":"2021","journal-title":"Biochem. Biophys. Res. 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Care"},{"issue":"6","key":"2026010813052254000_cit32","doi-asserted-by":"crossref","first-page":"979","DOI":"10.1016\/j.bone.2007.08.034","article-title":"Polymorphisms and haplotypes of integrinalpha1 (ITGA1) are associated with bone mineral density and fracture risk in postmenopausal Koreans","volume":"41","author":"Lee","year":"2007","journal-title":"Bone"},{"issue":"9\u201310","key":"2026010813052254000_cit33","doi-asserted-by":"crossref","first-page":"546","DOI":"10.1016\/j.bbaexp.2007.06.004","article-title":"Transcriptional and epigenetic regulation of the integrin collagen receptor locus ITGA1-PELO-ITGA2","volume":"1769","author":"Cheli","year":"2007","journal-title":"Biochim. Biophys. Acta"},{"issue":"6","key":"2026010813052254000_cit34","doi-asserted-by":"crossref","first-page":"979","DOI":"10.1016\/j.bone.2007.08.034","article-title":"Polymorphisms and haplotypes of integrinalpha1 (ITGA1) are associated with bone mineral density and fracture risk in postmenopausal Koreans","volume":"41","author":"Lee","year":"2007","journal-title":"Bone"}],"updated-by":[{"DOI":"10.1039\/d3mo90016a","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2023,5,24]],"date-time":"2023-05-24T00:00:00Z","timestamp":1684886400000}}],"container-title":["Molecular Omics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/molecular-omics\/article-pdf\/19\/6\/454\/65027545\/d3mo00018d.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/molecular-omics\/article-pdf\/19\/6\/454\/65027545\/d3mo00018d.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T18:06:54Z","timestamp":1767895614000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/molecular-omics\/article\/19\/6\/454\/8277220"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4,26]]},"references-count":34,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2023,4,26]]}},"URL":"https:\/\/doi.org\/10.1039\/d3mo00018d","relation":{},"ISSN":["2515-4184"],"issn-type":[{"value":"2515-4184","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2023,7,1]]},"published":{"date-parts":[[2023,4,26]]}}}