{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T23:37:28Z","timestamp":1790293048168,"version":"4.1.0"},"reference-count":42,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T00:00:00Z","timestamp":1764115200000},"content-version":"vor","delay-in-days":608,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,3,28]],"date-time":"2024-03-28T00:00:00Z","timestamp":1711584000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["105603\/Z\/14\/Z"],"award-info":[{"award-number":["105603\/Z\/14\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["105603\/Z\/14\/Z"],"award-info":[{"award-number":["105603\/Z\/14\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["rsc.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2024,3,28]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Glycosyl-inositol-phospho-ceramides (GIPCs) or glycosylphosphatidylinositol-anchored fungal polysaccharides are major lipids in plant and fungal plasma membranes and play an important role in stress adaption. However, their analysis remains challenging due to the multiple steps involved in their extraction and purification prior to mass spectrometry analysis. To address this challenge, we report here a novel simplified method to identify GIPCs from Aspergillus fumigatus using the new Bruker MBT lipid Xtract assay. A. fumigatus reference strains and clinical isolates were cultured, harvested, heat-inactivated and suspended in double-distilled water. A fraction of this fungal preparation was then dried in a microtube, mixed with an MBT lipid Xtract matrix (Bruker Daltonik, Germany) and loaded onto a MALDI target plate. Analysis was performed using a Bruker MALDI Biotyper Sirius system in the linear negative ion mode. Mass spectra were scanned from m\/z 700 to m\/z 2\u2009000. MALDI-TOF MS analysis of cultured fungi showed a clear signature of GIPCs in Aspergillus fumigatus reference strains and clinical isolates. Here, we have demonstrated that routine MALDI-TOF in the linear negative ion mode combined with the MBT lipid Xtract is able to detect Aspergillus fumigatus GIPCs.<\/jats:p>\n                  <jats:p\/>","DOI":"10.1039\/d4mo00030g","type":"journal-article","created":{"date-parts":[[2024,3,28]],"date-time":"2024-03-28T07:41:01Z","timestamp":1711611661000},"page":"390-396","update-policy":"https:\/\/doi.org\/10.1039\/rsc_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Intact cell lipidomics using the Bruker MBT lipid Xtract assay allows the rapid detection of glycosyl-inositol-phospho-ceramides from\n                    <i>Aspergillus fumigatus<\/i>"],"prefix":"10.1093","volume":"20","author":[{"given":"Aishani","family":"Chakraborty","sequence":"first","affiliation":[{"name":"Centre for Bacterial Resistance Biology, Department of Life Sciences, Faculty of Natural Sciences, Imperial College London a , London, SW7 2AZ, UK \u00a0 g.larrouy-maumus@imperial.ac.uk"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leila","family":"Alsharqi","sequence":"additional","affiliation":[{"name":"Centre for Bacterial Resistance Biology, Department of Infectious Diseases, Faculty of Medicine, Imperial College London b , London, SW7 2AZ, UK \u00a0 d.armstrong@imperial.ac.uk"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Markus","family":"Kostrzewa","sequence":"additional","affiliation":[{"name":"Bruker Daltonik GmbH c , Bremen, 28359, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Darius","family":"Armstrong-James","sequence":"additional","affiliation":[{"name":"Centre for Bacterial Resistance Biology, Department of Infectious Diseases, Faculty of Medicine, Imperial College London b , London, SW7 2AZ, UK \u00a0 d.armstrong@imperial.ac.uk"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6614-8698","authenticated-orcid":false,"given":"Gerald","family":"Larrouy-Maumus","sequence":"additional","affiliation":[{"name":"Centre for Bacterial Resistance Biology, Department of Life Sciences, Faculty of Natural Sciences, Imperial College London a , London, SW7 2AZ, UK \u00a0 g.larrouy-maumus@imperial.ac.uk"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,3,28]]},"reference":[{"issue":"2","key":"2026010813055076500_cit1","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1128\/CMR.12.2.310","article-title":"Aspergillus fumigatus and aspergillosis","volume":"12","author":"Latge","year":"1999","journal-title":"Clin. Microbiol. Rev."},{"key":"2026010813055076500_cit2","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1146\/annurev-micro-030117-020406","article-title":"The Cell Wall of the Human Fungal Pathogen Aspergillus fumigatus: Biosynthesis, Organization, Immune Response, and Virulence","volume":"71","author":"Latge","year":"2017","journal-title":"Annu. Rev. Microbiol."},{"issue":"4","key":"2026010813055076500_cit3","doi-asserted-by":"crossref","first-page":"283","DOI":"10.3390\/jof6040283","article-title":"Galactomannan Produced by Aspergillus fumigatus: An Update on the Structure, Biosynthesis and Biological Functions of an Emblematic Fungal Biomarker","volume":"6","author":"Fontaine","year":"2020","journal-title":"J. Fungi."},{"issue":"4","key":"2026010813055076500_cit4","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1128\/mSphere.00397-19","article-title":"The Glycosylphosphatidylinositol-Anchored DFG Family Is Essential for the Insertion of Galactomannan into the beta-(1,3)-Glucan-Chitin Core of the Cell Wall of Aspergillus fumigatus","volume":"4","author":"Muszkieta","year":"2019","journal-title":"mSphere."},{"issue":"1","key":"2026010813055076500_cit5","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1093\/glycob\/cwm122","article-title":"Glycosylinositolphosphoceramides in Aspergillus fumigatus","volume":"18","author":"Simenel","year":"2008","journal-title":"Glycobiology"},{"issue":"48","key":"2026010813055076500_cit6","doi-asserted-by":"crossref","first-page":"39835","DOI":"10.1074\/jbc.M510163200","article-title":"Glycosylphosphatidylinositol-anchored fungal polysaccharide in Aspergillus fumigatus","volume":"280","author":"Costachel","year":"2005","journal-title":"J. Biol. Chem."},{"issue":"3","key":"2026010813055076500_cit7","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1093\/glycob\/cwg004","article-title":"Structures of the glycosylphosphatidylinositol membrane anchors from Aspergillus fumigatus membrane proteins","volume":"13","author":"Fontaine","year":"2003","journal-title":"Glycobiology"},{"issue":"32","key":"2026010813055076500_cit8","doi-asserted-by":"crossref","first-page":"22684","DOI":"10.1074\/jbc.M604050200","article-title":"Separation and identification of major plant sphingolipid classes from leaves","volume":"281","author":"Markham","year":"2006","journal-title":"J. Biol. Chem."},{"issue":"4","key":"2026010813055076500_cit9","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1007\/s00216-013-7130-8","article-title":"Characterization of glycosyl inositol phosphoryl ceramides from plants and fungi by mass spectrometry","volume":"406","author":"Bure","year":"2014","journal-title":"Anal. Bioanal. Chem."},{"issue":"1","key":"2026010813055076500_cit10","doi-asserted-by":"crossref","first-page":"19","DOI":"10.3390\/jof4010019","article-title":"Glycosylphosphatidylinositol Anchors from Galactomannan and GPI-Anchored Protein Are Synthesized by Distinct Pathways in Aspergillus fumigatus","volume":"4","author":"Li","year":"2018","journal-title":"J. Fungi."},{"issue":"8","key":"2026010813055076500_cit11","doi-asserted-by":"crossref","first-page":"1268","DOI":"10.1128\/EC.00109-08","article-title":"Contribution of galactofuranose to the virulence of the opportunistic pathogen Aspergillus fumigatus","volume":"7","author":"Schmalhorst","year":"2008","journal-title":"Eukaryotic Cell"},{"issue":"26","key":"2026010813055076500_cit12","doi-asserted-by":"crossref","first-page":"12147","DOI":"10.1016\/S0021-9258(18)67215-1","article-title":"Identification of a glycolipid precursor of the Trypanosoma brucei variant surface glycoprotein","volume":"261","author":"Krakow","year":"1986","journal-title":"J. Biol. Chem."},{"key":"2026010813055076500_cit13","doi-asserted-by":"crossref","first-page":"791","DOI":"10.3389\/fmicb.2015.00791","article-title":"MALDI-TOF mass spectrometry: an emerging technology for microbial identification and diagnosis","volume":"6","author":"Singhal","year":"2015","journal-title":"Front. 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Lipid Res."},{"issue":"23","key":"2026010813055076500_cit17","doi-asserted-by":"crossref","first-page":"2431","DOI":"10.1016\/j.carres.2003.07.010","article-title":"Structure of bacterial lipopolysaccharides","volume":"338","author":"Caroff","year":"2003","journal-title":"Carbohydr. Res."},{"issue":"3","key":"2026010813055076500_cit18","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1042\/bst0090191","article-title":"Bacterial lipopolysaccharide and its lipid A component: some historical and some current aspects","volume":"9","author":"Westphal","year":"1981","journal-title":"Biochem. Soc. Trans."},{"issue":"21","key":"2026010813055076500_cit19","first-page":"17","article-title":"[Chemical and biological analysis of highly purified bacterial polysaccharides]","volume":"78","author":"Westphal","year":"1953","journal-title":"Dtsch. Med. Wochenschr."},{"key":"2026010813055076500_cit20","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/S0076-6879(10)80005-9","article-title":"Microbe-associated molecular patterns in innate immunity: Extraction and chemical analysis of Gram-negative bacterial lipopolysaccharides","volume":"480","author":"De Castro","year":"2010","journal-title":"Methods Enzymol."},{"issue":"13","key":"2026010813055076500_cit21","doi-asserted-by":"crossref","first-page":"1829","DOI":"10.1002\/rcm.1994","article-title":"New conditions for matrix-assisted laser desorption\/ionization mass spectrometry of native bacterial R-type lipopolysaccharides","volume":"19","author":"Sturiale","year":"2005","journal-title":"Rapid Commun. 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Dis."},{"issue":"4","key":"2026010813055076500_cit23","doi-asserted-by":"crossref","first-page":"e1313","DOI":"10.1002\/mbo3.1313","article-title":"Discrimination of Escherichia coli, Shigella flexneri, and Shigella sonnei using lipid profiling by MALDI-TOF mass spectrometry paired with machine learning","volume":"11","author":"Pizzato","year":"2022","journal-title":"MicrobiologyOpen"},{"key":"2026010813055076500_cit24","doi-asserted-by":"crossref","first-page":"725383","DOI":"10.3389\/fmicb.2021.725383","article-title":"Detection of Colistin Resistance in Pseudomonas aeruginosa Using the MALDIxin Test on the Routine MALDI Biotyper Sirius Mass Spectrometer","volume":"12","author":"Jeannot","year":"2021","journal-title":"Front. Microbiol."},{"issue":"4","key":"2026010813055076500_cit25","doi-asserted-by":"crossref","first-page":"e1008331","DOI":"10.1371\/journal.ppat.1008331","article-title":"The clue is in the lipid A: Rapid detection of colistin resistance","volume":"16","author":"Furniss","year":"2020","journal-title":"PLoS Pathog."},{"issue":"1","key":"2026010813055076500_cit26","doi-asserted-by":"crossref","first-page":"16910","DOI":"10.1038\/s41598-018-35041-y","article-title":"Rapid detection of colistin resistance in Acinetobacter baumannii using MALDI-TOF-based lipidomics on intact bacteria","volume":"8","author":"Dortet","year":"2018","journal-title":"Sci. Rep."},{"issue":"12","key":"2026010813055076500_cit27","first-page":"3359","article-title":"Rapid detection and discrimination of chromosome- and MCR-plasmid-mediated resistance to polymyxins by MALDI-TOF MS in Escherichia coli: the MALDIxin test","volume":"73","author":"Dortet","year":"2018","journal-title":"J. Antimicrob. Chemother."},{"issue":"1","key":"2026010813055076500_cit28","doi-asserted-by":"crossref","first-page":"21536","DOI":"10.1038\/s41598-020-78401-3","article-title":"Rapid microbial identification and colistin resistance detection via MALDI-TOF MS using a novel on-target extraction of membrane lipids","volume":"10","author":"Sorensen","year":"2020","journal-title":"Sci. Rep."},{"issue":"2","key":"2026010813055076500_cit29","doi-asserted-by":"crossref","first-page":"1286","DOI":"10.1021\/acs.analchem.8b02611","article-title":"Rapid Microbial Identification and Antibiotic Resistance Detection by Mass Spectrometric Analysis of Membrane Lipids","volume":"91","author":"Liang","year":"2019","journal-title":"Anal. Chem."},{"issue":"11","key":"2026010813055076500_cit30","doi-asserted-by":"crossref","first-page":"3035","DOI":"10.1093\/jac\/dkx234","article-title":"Structural modification of LPS in colistin-resistant, KPC-producing Klebsiella pneumoniae","volume":"72","author":"Leung","year":"2017","journal-title":"J. Antimicrob. Chemother."},{"issue":"1","key":"2026010813055076500_cit31","doi-asserted-by":"crossref","first-page":"6403","DOI":"10.1038\/s41598-017-04793-4","article-title":"Identification of the ESKAPE pathogens by mass spectrometric analysis of microbial membrane glycolipids","volume":"7","author":"Leung","year":"2017","journal-title":"Sci. Rep."},{"key":"2026010813055076500_cit32","doi-asserted-by":"crossref","first-page":"1141","DOI":"10.3389\/fmicb.2020.01141","article-title":"Detection of Colistin Resistance in Salmonella enterica Using MALDIxin Test on the Routine MALDI Biotyper Sirius Mass Spectrometer","volume":"11","author":"Dortet","year":"2020","journal-title":"Front. Microbiol."},{"key":"2026010813055076500_cit33","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1128\/JCM.01427-19","article-title":"Detection of Colistin Resistance in Escherichia coli by Use of the MALDI Biotyper Sirius Mass Spectrometry System","volume":"57","author":"Furniss","year":"2019","journal-title":"J. Clin. Microbiol."},{"issue":"9","key":"2026010813055076500_cit34","doi-asserted-by":"crossref","first-page":"2544","DOI":"10.1093\/jac\/dkz241","article-title":"ISAba1-dependent overexpression of eptA in clinical strains of Acinetobacter baumannii resistant to colistin","volume":"74","author":"Potron","year":"2019","journal-title":"J. Antimicrob. Chemother."},{"issue":"6","key":"2026010813055076500_cit35","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1016\/j.cmi.2018.09.008","article-title":"How to: EUCAST recommendations on the screening procedure E.Def 10.1 for the detection of azole resistance in Aspergillus fumigatus isolates using four-well azole-containing agar plates","volume":"25","author":"Guinea","year":"2019","journal-title":"Clin. Microbiol. Infect."},{"issue":"6","key":"2026010813055076500_cit36","doi-asserted-by":"crossref","first-page":"e0218951","DOI":"10.1371\/journal.pone.0218951","article-title":"MALDI-TOF mass spectrometry on intact bacteria combined with a refined analysis framework allows accurate classification of MSSA and MRSA","volume":"14","author":"Tang","year":"2019","journal-title":"PLoS One"},{"key":"2026010813055076500_cit37","author":"Gibb","year":"2012"},{"issue":"53","key":"2026010813055076500_cit38","doi-asserted-by":"crossref","first-page":"44418","DOI":"10.1074\/jbc.M112.398321","article-title":"Biosynthesis of the fungal cell wall polysaccharide galactomannan requires intraluminal GDP-mannose","volume":"287","author":"Engel","year":"2012","journal-title":"J. Biol. Chem."},{"issue":"12","key":"2026010813055076500_cit39","doi-asserted-by":"crossref","first-page":"1423","DOI":"10.1093\/glycob\/cwv059","article-title":"Characterization of an N-acetylglucosaminyltransferase involved in Aspergillus fumigatus zwitterionic glycoinositolphosphoceramide biosynthesis","volume":"25","author":"Engel","year":"2015","journal-title":"Glycobiology"},{"issue":"8","key":"2026010813055076500_cit40","doi-asserted-by":"crossref","first-page":"1801","DOI":"10.1194\/jlr.M700149-JLR200","article-title":"Analysis of glycosylinositol phosphorylceramides expressed by the opportunistic mycopathogen Aspergillus fumigatus","volume":"48","author":"Toledo","year":"2007","journal-title":"J. Lipid Res."},{"key":"2026010813055076500_cit41","first-page":"213","article-title":"Analysis of the carbohydrate and lipid components of glycosylphosphatidylinositol structures","volume":"76","author":"Treumann","year":"1998","journal-title":"Methods Mol. Biol."},{"key":"2026010813055076500_cit42","doi-asserted-by":"crossref","first-page":"1156795","DOI":"10.3389\/fmicb.2023.1156795","article-title":"A whole cell-based Matrix-assisted laser desorption\/ionization mass spectrometry lipidomic assay for the discovery of compounds that target lipid a modifications","volume":"14","author":"Tang","year":"2023","journal-title":"Front. Microbiol."}],"container-title":["Molecular Omics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/molecular-omics\/article-pdf\/20\/6\/390\/65027365\/d4mo00030g.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/molecular-omics\/article-pdf\/20\/6\/390\/65027365\/d4mo00030g.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T18:07:27Z","timestamp":1767895647000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/molecular-omics\/article\/20\/6\/390\/8277266"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,3,28]]},"references-count":42,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2024,3,28]]}},"URL":"https:\/\/doi.org\/10.1039\/d4mo00030g","relation":{},"ISSN":["2515-4184"],"issn-type":[{"value":"2515-4184","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2024,7,1]]},"published":{"date-parts":[[2024,3,28]]}}}