{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T19:27:02Z","timestamp":1789586822448,"version":"build-2803163510"},"reference-count":66,"publisher":"Royal Society of Chemistry (RSC)","issue":"7","license":[{"start":{"date-parts":[[2025,1,22]],"date-time":"2025-01-22T00:00:00Z","timestamp":1737504000000},"content-version":"vor","delay-in-days":21,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100005155","name":"Azrieli Foundation","doi-asserted-by":"publisher","award":["Azrieli International Postdoctoral Fellowship"],"award-info":[{"award-number":["Azrieli International Postdoctoral Fellowship"]}],"id":[{"id":"10.13039\/501100005155","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["Projektnummer 490757872"],"award-info":[{"award-number":["Projektnummer 490757872"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010663","name":"H2020 European Research Council","doi-asserted-by":"publisher","award":["grant agreement No 742637, SMALLOSTERY"],"award-info":[{"award-number":["grant agreement No 742637, SMALLOSTERY"]}],"id":[{"id":"10.13039\/100010663","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003977","name":"Israel Science Foundation","doi-asserted-by":"publisher","award":["no. 1250\/19"],"award-info":[{"award-number":["no. 1250\/19"]}],"id":[{"id":"10.13039\/501100003977","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001735","name":"Weizmann Institute of Science","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001735","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["rsc.org"],"crossmark-restriction":true},"short-container-title":["Chem. Sci."],"abstract":"<jats:p>Single-molecule FRET reveals urea-induced conformational shifts in adenylate kinase, enhancing activity through promoting the open conformation and efficient substrate positioning.<\/jats:p>","DOI":"10.1039\/d4sc06819j","type":"journal-article","created":{"date-parts":[[2025,1,22]],"date-time":"2025-01-22T04:20:41Z","timestamp":1737519641000},"page":"3066-3077","update-policy":"https:\/\/doi.org\/10.1039\/rsc_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Interplay between conformational dynamics and substrate binding regulates enzymatic activity: a single-molecule FRET study"],"prefix":"10.1039","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6641-6629","authenticated-orcid":false,"given":"David","family":"Scheerer","sequence":"first","affiliation":[{"name":"Department of Chemical and Biological Physics, Weizmann Institute of Science, Rehovot 761001, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dorit","family":"Levy","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biological Physics, Weizmann Institute of Science, Rehovot 761001, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Remi","family":"Casier","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biological Physics, Weizmann Institute of Science, Rehovot 761001, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inbal","family":"Riven","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biological Physics, Weizmann Institute of Science, Rehovot 761001, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hisham","family":"Mazal","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biological Physics, Weizmann Institute of Science, Rehovot 761001, Israel"},{"name":"Max Planck Institute for the Science of Light, Erlangen 91058, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1837-9779","authenticated-orcid":false,"given":"Gilad","family":"Haran","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biological Physics, Weizmann Institute of Science, Rehovot 761001, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"292","published-online":{"date-parts":[[2025]]},"reference":[{"key":"D4SC06819J\/cit1\/1","doi-asserted-by":"crossref","first-page":"6788","DOI":"10.1021\/acs.chemrev.8b00760","volume":"119","author":"Thirumalai","year":"2019","journal-title":"Chem. Rev."},{"key":"D4SC06819J\/cit2\/1","doi-asserted-by":"crossref","first-page":"180901","DOI":"10.1063\/1.4947037","volume":"144","author":"Warshel","year":"2016","journal-title":"J. Chem. Phys."},{"key":"D4SC06819J\/cit3\/1","doi-asserted-by":"crossref","first-page":"964","DOI":"10.1038\/nature06522","volume":"450","author":"Henzler-Wildman","year":"2007","journal-title":"Nature"},{"key":"D4SC06819J\/cit4\/1","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1021\/ar5002928","volume":"48","author":"Callender","year":"2015","journal-title":"Acc. Chem. Res."},{"key":"D4SC06819J\/cit5\/1","doi-asserted-by":"crossref","first-page":"6425","DOI":"10.1021\/bi952687j","volume":"35","author":"Sinev","year":"1996","journal-title":"Biochemistry"},{"key":"D4SC06819J\/cit6\/1","doi-asserted-by":"crossref","first-page":"838","DOI":"10.1038\/nature06410","volume":"450","author":"Henzler-Wildman","year":"2007","journal-title":"Nature"},{"key":"D4SC06819J\/cit7\/1","doi-asserted-by":"crossref","first-page":"16984","DOI":"10.1073\/pnas.0906510106","volume":"106","author":"Schrank","year":"2009","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"D4SC06819J\/cit8\/1","doi-asserted-by":"crossref","first-page":"7644","DOI":"10.1038\/ncomms8644","volume":"6","author":"Kovermann","year":"2015","journal-title":"Nat. Commun."},{"key":"D4SC06819J\/cit9\/1","doi-asserted-by":"crossref","first-page":"127","DOI":"10.2152\/jmi.52.127","volume":"52","author":"Noma","year":"2005","journal-title":"J. Med. Invest."},{"key":"D4SC06819J\/cit10\/1","doi-asserted-by":"crossref","first-page":"1729","DOI":"10.3390\/ijms10041729","volume":"10","author":"Dzeja","year":"2009","journal-title":"Int. J. Mol. Sci."},{"key":"D4SC06819J\/cit11\/1","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1016\/S0022-2836(05)80250-5","volume":"213","author":"Schulz","year":"1990","journal-title":"J. Mol. Biol."},{"key":"D4SC06819J\/cit12\/1","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/0022-2836(92)90582-5","volume":"224","author":"M\u00fcller","year":"1992","journal-title":"J. Mol. Biol."},{"key":"D4SC06819J\/cit13\/1","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1016\/S0969-2126(01)00181-2","volume":"3","author":"Vonrhein","year":"1995","journal-title":"Structure"},{"key":"D4SC06819J\/cit14\/1","doi-asserted-by":"crossref","first-page":"945","DOI":"10.1038\/nsmb821","volume":"11","author":"Wolf-Watz","year":"2004","journal-title":"Nat. Struct. Mol. Biol."},{"key":"D4SC06819J\/cit15\/1","doi-asserted-by":"crossref","first-page":"18055","DOI":"10.1073\/pnas.0708600104","volume":"104","author":"Hanson","year":"2007","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"D4SC06819J\/cit16\/1","doi-asserted-by":"crossref","first-page":"3243","DOI":"10.1073\/pnas.1720448115","volume":"115","author":"Aviram","year":"2018","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"D4SC06819J\/cit17\/1","doi-asserted-by":"crossref","first-page":"e2219855120","DOI":"10.1073\/pnas.2219855120","volume":"120","author":"Scheerer","year":"2023","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"D4SC06819J\/cit18\/1","doi-asserted-by":"crossref","first-page":"3211","DOI":"10.1021\/acs.jctc.5b00268","volume":"11","author":"Lee","year":"2015","journal-title":"J. Chem. Theor. Comput."},{"key":"D4SC06819J\/cit19\/1","doi-asserted-by":"crossref","first-page":"238102","DOI":"10.1103\/PhysRevLett.122.238102","volume":"122","author":"Li","year":"2019","journal-title":"Phys. Rev. Lett."},{"key":"D4SC06819J\/cit20\/1","doi-asserted-by":"crossref","first-page":"1716","DOI":"10.1021\/acs.jctc.7b01064","volume":"14","author":"Zheng","year":"2018","journal-title":"J. Chem. Theory Comput."},{"key":"D4SC06819J\/cit21\/1","doi-asserted-by":"crossref","first-page":"1661","DOI":"10.1016\/j.jmb.2006.11.085","volume":"366","author":"Whitford","year":"2007","journal-title":"J. Mol. Biol."},{"key":"D4SC06819J\/cit22\/1","doi-asserted-by":"crossref","first-page":"8425","DOI":"10.1038\/srep08425","volume":"5","author":"Formoso","year":"2015","journal-title":"Sci. Rep."},{"key":"D4SC06819J\/cit23\/1","doi-asserted-by":"crossref","first-page":"807","DOI":"10.1016\/j.jmb.2005.07.031","volume":"352","author":"Maragakis","year":"2005","journal-title":"J. Mol. Biol."},{"key":"D4SC06819J\/cit24\/1","doi-asserted-by":"crossref","first-page":"6792","DOI":"10.1021\/acs.jpcb.2c03832","volume":"126","author":"Zhang","year":"2022","journal-title":"J. Phys. Chem. B"},{"key":"D4SC06819J\/cit25\/1","doi-asserted-by":"crossref","first-page":"8188","DOI":"10.1021\/acs.jpcb.2c05497","volume":"126","author":"Lu","year":"2022","journal-title":"J. Phys. Chem. B"},{"key":"D4SC06819J\/cit26\/1","doi-asserted-by":"crossref","first-page":"2246","DOI":"10.1021\/acs.biochem.1c00221","volume":"60","author":"Ojeda-May","year":"2021","journal-title":"Biochemistry"},{"issue":"5","key":"D4SC06819J\/cit27\/1","doi-asserted-by":"crossref","first-page":"1556","DOI":"10.1021\/acs.jcim.2c01629","volume":"63","author":"Dulko-Smith","year":"2023","journal-title":"J. Chem. Inf. Model."},{"key":"D4SC06819J\/cit28\/1","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1021\/acs.jcim.3c01618","volume":"64","author":"Nam","year":"2024","journal-title":"J. Chem. Inf. Model."},{"key":"D4SC06819J\/cit29\/1","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1006\/bbrc.1997.7301","volume":"238","author":"Zhang","year":"1997","journal-title":"Biochem. Biophys. Res."},{"key":"D4SC06819J\/cit30\/1","doi-asserted-by":"crossref","first-page":"1385","DOI":"10.1016\/j.bpj.2016.08.028","volume":"111","author":"Rogne","year":"2016","journal-title":"Biophys. J."},{"key":"D4SC06819J\/cit31\/1","doi-asserted-by":"crossref","first-page":"109658","DOI":"10.1016\/j.abb.2023.109658","author":"Rastogi","year":"2023","journal-title":"Arch. Biochem. Biophys."},{"key":"D4SC06819J\/cit32\/1","doi-asserted-by":"crossref","first-page":"2658","DOI":"10.1038\/srep02658","volume":"3","author":"Cao","year":"2013","journal-title":"Sci. Rep."},{"key":"D4SC06819J\/cit33\/1","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1002\/prot.340090105","volume":"9","author":"Liang","year":"1991","journal-title":"Proteins: Struct., Funct., Bioinf."},{"key":"D4SC06819J\/cit34\/1","doi-asserted-by":"crossref","first-page":"13065","DOI":"10.1021\/acs.jpcb.6b10726","volume":"120","author":"Pirchi","year":"2016","journal-title":"J. Phys. Chem. B"},{"key":"D4SC06819J\/cit35\/1","first-page":"100116","author":"Terterov","year":"2023","journal-title":"Biophys. Rep."},{"key":"D4SC06819J\/cit36\/1","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/S0014-5793(96)01195-7","volume":"397","author":"Sinev","year":"1996","journal-title":"FEBS Lett."},{"key":"D4SC06819J\/cit37\/1","doi-asserted-by":"crossref","first-page":"10109","DOI":"10.1038\/s41467-024-54421-9","volume":"15","author":"Maglia","year":"2024","journal-title":"Nat. Commun."},{"key":"D4SC06819J\/cit38\/1","doi-asserted-by":"crossref","first-page":"e1002555","DOI":"10.1371\/journal.pcbi.1002555","volume":"8","author":"Matsunaga","year":"2012","journal-title":"PLoS Comput. Biol."},{"key":"D4SC06819J\/cit39\/1","doi-asserted-by":"crossref","first-page":"6511","DOI":"10.1016\/S0021-9258(19)34144-4","volume":"246","author":"Lowry","year":"1971","journal-title":"J. Biol. Chem."},{"key":"D4SC06819J\/cit40\/1","doi-asserted-by":"crossref","first-page":"4391","DOI":"10.1073\/pnas.1318122111","volume":"111","author":"Reuveni","year":"2014","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"D4SC06819J\/cit41\/1","doi-asserted-by":"crossref","first-page":"3437","DOI":"10.1021\/acs.jpcb.6b09055","volume":"121","author":"Berezhkovskii","year":"2017","journal-title":"J. Phys. Chem. B"},{"key":"D4SC06819J\/cit42\/1","doi-asserted-by":"crossref","first-page":"2573","DOI":"10.1016\/j.bbapap.2013.09.009","volume":"1834","author":"Franklin","year":"2013","journal-title":"Biochim. Biophys. Acta, Proteins Proteomics"},{"key":"D4SC06819J\/cit43\/1","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/0167-4838(95)00125-E","volume":"1252","author":"Fan","year":"1995","journal-title":"Biochim. Biophys. Acta Protein Struct. Mol. Enzymol."},{"key":"D4SC06819J\/cit44\/1","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1042\/bj3150097","volume":"315","author":"Fan","year":"1996","journal-title":"Biochem. J."},{"key":"D4SC06819J\/cit45\/1","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1006\/bbrc.1999.1881","volume":"266","author":"Inui","year":"1999","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"D4SC06819J\/cit46\/1","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1042\/bj0980278","volume":"98","author":"Ferdinand","year":"1966","journal-title":"Biochem. J."},{"key":"D4SC06819J\/cit47\/1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1749-6632.1998.tb10282.x","volume":"864","author":"Tsou","year":"1998","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"D4SC06819J\/cit48\/1","doi-asserted-by":"crossref","first-page":"2845","DOI":"10.1074\/jbc.M209934200","volume":"278","author":"Inui","year":"2003","journal-title":"J. Biol. Chem."},{"key":"D4SC06819J\/cit49\/1","doi-asserted-by":"crossref","first-page":"4687","DOI":"10.1021\/acs.jpcb.3c00705","volume":"127","author":"Haran","year":"2023","journal-title":"J. Phys. Chem. B"},{"key":"D4SC06819J\/cit50\/1","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1146\/annurev-biophys-070323-022428","volume":"50","author":"Schanda","year":"2024","journal-title":"Annu. Rev. Biophys."},{"key":"D4SC06819J\/cit51\/1","doi-asserted-by":"crossref","first-page":"2748","DOI":"10.1038\/s41467-021-22968-6","volume":"12","author":"Wurm","year":"2021","journal-title":"Nat. Commun."},{"key":"D4SC06819J\/cit52\/1","doi-asserted-by":"crossref","first-page":"e2310910120","DOI":"10.1073\/pnas.2310910120","volume":"120","author":"Shukla","year":"2023","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"D4SC06819J\/cit53\/1","doi-asserted-by":"crossref","first-page":"17314","DOI":"10.1021\/jacs.9b08952","volume":"141","author":"Shi","year":"2019","journal-title":"J. Am. Chem. Soc."},{"key":"D4SC06819J\/cit54\/1","doi-asserted-by":"crossref","first-page":"1438","DOI":"10.1038\/s41467-019-09474-6","volume":"10","author":"Mazal","year":"2019","journal-title":"Nat. Commun."},{"key":"D4SC06819J\/cit55\/1","doi-asserted-by":"crossref","first-page":"11265","DOI":"10.1073\/pnas.1821447116","volume":"116","author":"Adkar","year":"2019","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"D4SC06819J\/cit56\/1","doi-asserted-by":"crossref","first-page":"12695","DOI":"10.1021\/bi00208a021","volume":"33","author":"Lin","year":"1994","journal-title":"Biochemistry"},{"key":"D4SC06819J\/cit57\/1","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1126\/science.158.3799.383","volume":"158","author":"MacMillen","year":"1967","journal-title":"Science"},{"key":"D4SC06819J\/cit58\/1","doi-asserted-by":"crossref","first-page":"553","DOI":"10.2307\/1540268","volume":"141","author":"Lutz","year":"1971","journal-title":"Biol. Bull."},{"key":"D4SC06819J\/cit59\/1","doi-asserted-by":"crossref","first-page":"2991","DOI":"10.1016\/j.bpj.2011.03.065","volume":"100","author":"Nagarajan","year":"2011","journal-title":"Biophys. J."},{"key":"D4SC06819J\/cit60\/1","doi-asserted-by":"crossref","first-page":"16562","DOI":"10.1021\/ja3032482","volume":"134","author":"\u00c5d\u00e9n","year":"2012","journal-title":"J. Am. Chem. Soc."},{"key":"D4SC06819J\/cit61\/1","doi-asserted-by":"crossref","first-page":"11324","DOI":"10.1021\/jacs.0c04924","volume":"142","author":"Crean","year":"2020","journal-title":"J. Am. Chem. Soc."},{"key":"D4SC06819J\/cit62\/1","doi-asserted-by":"crossref","first-page":"22238","DOI":"10.1074\/jbc.274.32.22238","volume":"274","author":"Sheng","year":"1999","journal-title":"J. Biol. Chem."},{"key":"D4SC06819J\/cit63\/1","doi-asserted-by":"crossref","first-page":"3508","DOI":"10.1529\/biophysj.105.064766","volume":"89","author":"M\u00fcller","year":"2005","journal-title":"Biophys. J."},{"key":"D4SC06819J\/cit64\/1","doi-asserted-by":"crossref","first-page":"2939","DOI":"10.1529\/biophysj.104.054114","volume":"88","author":"Lee","year":"2005","journal-title":"Biophys. J."},{"key":"D4SC06819J\/cit65\/1","doi-asserted-by":"crossref","first-page":"1156","DOI":"10.1039\/C3CS60233H","volume":"43","author":"Hohlbein","year":"2014","journal-title":"Chem. Soc. Rev."},{"key":"D4SC06819J\/cit66\/1","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1038\/s41592-018-0085-0","volume":"15","author":"Hellenkamp","year":"2018","journal-title":"Nat. Methods"}],"container-title":["Chemical Science"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/pubs.rsc.org\/en\/content\/articlepdf\/2025\/SC\/D4SC06819J","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T17:29:21Z","timestamp":1783013361000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.rsc.org\/sc\/article\/16\/7\/3066-3077\/870206"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025]]},"references-count":66,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2025,2,12]]}},"URL":"https:\/\/doi.org\/10.1039\/d4sc06819j","relation":{"has-review":[{"id-type":"doi","id":"10.1039\/D4SC06819J\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1039\/D4SC06819J\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1039\/D4SC06819J\/v1\/review3","asserted-by":"object"},{"id-type":"doi","id":"10.1039\/D4SC06819J\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1039\/D4SC06819J\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1039\/D4SC06819J\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1039\/D4SC06819J\/v1\/decision1","asserted-by":"object"}]},"ISSN":["2041-6520","2041-6539"],"issn-type":[{"value":"2041-6520","type":"print"},{"value":"2041-6539","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025]]}}}