{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T15:47:28Z","timestamp":1787240848662,"version":"build-2736575974"},"reference-count":45,"publisher":"Royal Society of Chemistry (RSC)","issue":"46","license":[{"start":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T00:00:00Z","timestamp":1793404800000},"content-version":"am","delay-in-days":668,"URL":"http:\/\/rsc.li\/journals-terms-of-use"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2024YFF0618100"],"award-info":[{"award-number":["2024YFF0618100"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"publisher","award":["LY23B050004"],"award-info":[{"award-number":["LY23B050004"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100022963","name":"Key Research and Development Program of Zhejiang Province","doi-asserted-by":"publisher","award":["2025C02125"],"award-info":[{"award-number":["2025C02125"]}],"id":[{"id":"10.13039\/100022963","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017599","name":"Science and Technology Program of Zhejiang Province","doi-asserted-by":"publisher","award":["2025SNJF021"],"award-info":[{"award-number":["2025SNJF021"]}],"id":[{"id":"10.13039\/501100017599","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017599","name":"Science and Technology Program of Zhejiang Province","doi-asserted-by":"publisher","award":["2024SNJF073"],"award-info":[{"award-number":["2024SNJF073"]}],"id":[{"id":"10.13039\/501100017599","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100022955","name":"Fundamental Research Funds for the Provincial Universities of Zhejiang","doi-asserted-by":"publisher","award":["2024YW55"],"award-info":[{"award-number":["2024YW55"]}],"id":[{"id":"10.13039\/100022955","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["rsc.org"],"crossmark-restriction":true},"short-container-title":["Anal. Methods"],"abstract":"<jats:p>An ultrasensitive electrochemical biosensor for parvovirus B19V DNA is presented, integrating CRISPR-Cas12a with framework nucleic acids to achieve an amplification-free detection limit of 2.19 fM.<\/jats:p>","DOI":"10.1039\/d5ay01285f","type":"journal-article","created":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T09:00:55Z","timestamp":1761901255000},"page":"9357-9364","update-policy":"https:\/\/doi.org\/10.1039\/rsc_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Ultrasensitive electrochemical detection of parvovirus B19 DNA by combining CRISPR-Cas12a and multivalent framework nucleic acids"],"prefix":"10.1039","volume":"17","author":[{"given":"Jicong","family":"Hao","sequence":"first","affiliation":[{"name":"Key Laboratory of Microbiological Metrology, Measurement & Bioproduct Quality Security, State Administration for Market Regulation, College of Life Science, China Jiliang University, Hangzhou, 310018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueyan","family":"Gong","sequence":"additional","affiliation":[{"name":"Key Laboratory of Microbiological Metrology, Measurement & Bioproduct Quality Security, State Administration for Market Regulation, College of Life Science, China Jiliang University, Hangzhou, 310018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueyuan","family":"Duan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Microbiological Metrology, Measurement & Bioproduct Quality Security, State Administration for Market Regulation, College of Life Science, China Jiliang University, Hangzhou, 310018, China"},{"name":"Air Force Medical Center, Pharmacy Department, Beijing, 100142, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4356-6368","authenticated-orcid":false,"given":"Weiwei","family":"Qin","sequence":"additional","affiliation":[{"name":"Key Laboratory of Microbiological Metrology, Measurement & Bioproduct Quality Security, State Administration for Market Regulation, College of Life Science, China Jiliang University, Hangzhou, 310018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiying","family":"Ren","sequence":"additional","affiliation":[{"name":"Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuping","family":"ShenTu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Microbiological Metrology, Measurement & Bioproduct Quality Security, State Administration for Market Regulation, College of Life Science, China Jiliang University, Hangzhou, 310018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zihong","family":"Ye","sequence":"additional","affiliation":[{"name":"Key Laboratory of Microbiological Metrology, Measurement & Bioproduct Quality Security, State Administration for Market Regulation, College of Life Science, China Jiliang University, Hangzhou, 310018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoping","family":"Yu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Microbiological Metrology, Measurement & Bioproduct Quality Security, State Administration for Market Regulation, College of Life Science, China Jiliang University, Hangzhou, 310018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"292","published-online":{"date-parts":[[2025]]},"reference":[{"key":"D5AY01285F\/cit1\/1","doi-asserted-by":"crossref","first-page":"107865","DOI":"10.1016\/j.ijid.2025.107865","volume":"154","author":"Akhtar","year":"2025","journal-title":"Int. J. Infect. Dis."},{"key":"D5AY01285F\/cit2\/1","first-page":"197","volume":"29","author":"Shabani","year":"2015","journal-title":"Med. J. Islam. Repub. Iran"},{"key":"D5AY01285F\/cit3\/1","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1016\/j.tracli.2021.12.005","volume":"29","author":"Zhong","year":"2022","journal-title":"Transfus. Clin. Biol."},{"key":"D5AY01285F\/cit4\/1","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1080\/14737159.2018.1503537","volume":"18","author":"Gallinella","year":"2018","journal-title":"Expert Rev. Mol. Diagn."},{"key":"D5AY01285F\/cit5\/1","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/S0213-005X(11)70039-8","volume":"29","author":"Mart\u00ednez","year":"2011","journal-title":"Enferm. Infecc. Microbiol. Clin."},{"key":"D5AY01285F\/cit6\/1","doi-asserted-by":"crossref","first-page":"113489","DOI":"10.1016\/j.jpba.2020.113489","volume":"190","author":"Wu","year":"2020","journal-title":"J. Pharm. Biomed. Anal."},{"key":"D5AY01285F\/cit7\/1","doi-asserted-by":"crossref","first-page":"8769","DOI":"10.1039\/c3cs60144g","volume":"42","author":"Almassian","year":"2013","journal-title":"Chem. Soc. Rev."},{"key":"D5AY01285F\/cit8\/1","doi-asserted-by":"crossref","first-page":"S33","DOI":"10.2460\/javma.24.11.0760","volume":"263","author":"Sipka","year":"2025","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"D5AY01285F\/cit9\/1","doi-asserted-by":"crossref","first-page":"214","DOI":"10.37201\/req\/004.2025","volume":"38","author":"Reina","year":"2025","journal-title":"Rev. Esp. Quimioter."},{"key":"D5AY01285F\/cit10\/1","doi-asserted-by":"crossref","first-page":"1285","DOI":"10.31557\/APJCP.2025.26.4.1285","volume":"26","author":"Rahema","year":"2025","journal-title":"Asian Pac. J. Cancer Prev."},{"key":"D5AY01285F\/cit11\/1","doi-asserted-by":"crossref","first-page":"102682","DOI":"10.1016\/j.jiph.2025.102682","volume":"18","author":"Salman","year":"2025","journal-title":"J. Infect. Public Health"},{"key":"D5AY01285F\/cit12\/1","doi-asserted-by":"crossref","first-page":"108860","DOI":"10.1016\/j.bioelechem.2024.108860","volume":"162","author":"Mao","year":"2025","journal-title":"Bioelectrochemistry"},{"key":"D5AY01285F\/cit13\/1","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1186\/s12985-023-02048-5","volume":"20","author":"Bahrulolum","year":"2023","journal-title":"Virol. J."},{"key":"D5AY01285F\/cit14\/1","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1021\/acssensors.9b02461","volume":"5","author":"Zhang","year":"2020","journal-title":"ACS Sens."},{"key":"D5AY01285F\/cit15\/1","doi-asserted-by":"crossref","first-page":"2100935","DOI":"10.1002\/smtd.202100935","volume":"5","author":"Su","year":"2021","journal-title":"Small Methods"},{"key":"D5AY01285F\/cit16\/1","doi-asserted-by":"crossref","first-page":"3098","DOI":"10.1039\/D4CC00290C","volume":"60","author":"Sun","year":"2024","journal-title":"Chem. Commun."},{"key":"D5AY01285F\/cit17\/1","doi-asserted-by":"crossref","first-page":"125663","DOI":"10.1016\/j.talanta.2024.125663","volume":"271","author":"Lei","year":"2024","journal-title":"Talanta"},{"key":"D5AY01285F\/cit18\/1","doi-asserted-by":"crossref","first-page":"130630","DOI":"10.1016\/j.snb.2021.130630","volume":"347","author":"Bu","year":"2021","journal-title":"Sens. Actuators, B"},{"key":"D5AY01285F\/cit19\/1","doi-asserted-by":"crossref","first-page":"3988","DOI":"10.1021\/acssensors.3c01338","volume":"8","author":"Li","year":"2023","journal-title":"ACS Sens."},{"key":"D5AY01285F\/cit20\/1","doi-asserted-by":"crossref","first-page":"15789","DOI":"10.1021\/acs.analchem.4c03959","volume":"96","author":"Gong","year":"2024","journal-title":"Anal. Chem."},{"key":"D5AY01285F\/cit21\/1","doi-asserted-by":"crossref","first-page":"4842","DOI":"10.3390\/ijms22094842","volume":"22","author":"Ding","year":"2021","journal-title":"Int. J. Mol. Sci."},{"key":"D5AY01285F\/cit22\/1","doi-asserted-by":"crossref","first-page":"140675","DOI":"10.1016\/j.ijbiomac.2025.140675","volume":"303","author":"Liu","year":"2025","journal-title":"Int. J. Biol. Macromol."},{"key":"D5AY01285F\/cit23\/1","doi-asserted-by":"crossref","first-page":"8377","DOI":"10.1093\/nar\/gkac578","volume":"50","author":"Rossetti","year":"2022","journal-title":"Nucleic Acids Res."},{"key":"D5AY01285F\/cit24\/1","doi-asserted-by":"crossref","first-page":"e202305536","DOI":"10.1002\/anie.202305536","volume":"62","author":"Li","year":"2023","journal-title":"Angew. Chem., Int. Ed."},{"key":"D5AY01285F\/cit25\/1","doi-asserted-by":"crossref","first-page":"7504","DOI":"10.1038\/s41467-023-43389-7","volume":"14","author":"Moon","year":"2023","journal-title":"Nat. Commun."},{"key":"D5AY01285F\/cit26\/1","doi-asserted-by":"crossref","first-page":"512","DOI":"10.1002\/cplu.201900118","volume":"84","author":"Su","year":"2019","journal-title":"ChemPlusChem"},{"key":"D5AY01285F\/cit27\/1","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.coelec.2019.01.001","volume":"14","author":"Li","year":"2019","journal-title":"Curr. Opin. Electrochem."},{"key":"D5AY01285F\/cit28\/1","doi-asserted-by":"crossref","first-page":"7131","DOI":"10.1002\/anie.201802701","volume":"57","author":"Liu","year":"2018","journal-title":"Angew. Chem., Int. Ed."},{"key":"D5AY01285F\/cit29\/1","doi-asserted-by":"crossref","first-page":"100725","DOI":"10.1016\/j.apmt.2020.100725","volume":"20","author":"Zhang","year":"2020","journal-title":"Appl. Mater. Today"},{"key":"D5AY01285F\/cit30\/1","doi-asserted-by":"crossref","first-page":"2107820","DOI":"10.1002\/adma.202107820","volume":"34","author":"Zhang","year":"2022","journal-title":"Adv. Mater."},{"key":"D5AY01285F\/cit31\/1","doi-asserted-by":"crossref","first-page":"903","DOI":"10.1021\/acssensors.8b00257","volume":"3","author":"Yang","year":"2018","journal-title":"ACS Sens."},{"key":"D5AY01285F\/cit32\/1","first-page":"345","volume":"52","author":"Lin","year":"2021","journal-title":"J. Sichuan Univ., Med. Sci. Ed."},{"key":"D5AY01285F\/cit33\/1","doi-asserted-by":"crossref","first-page":"102127","DOI":"10.1016\/j.nantod.2023.102127","volume":"54","author":"Qi","year":"2024","journal-title":"Nano Today"},{"key":"D5AY01285F\/cit34\/1","doi-asserted-by":"crossref","first-page":"2410162","DOI":"10.1002\/smll.202410162","volume":"21","author":"Yang","year":"2025","journal-title":"Small"},{"key":"D5AY01285F\/cit35\/1","doi-asserted-by":"crossref","first-page":"1130","DOI":"10.1007\/s11427-019-1621-0","volume":"63","author":"Li","year":"2020","journal-title":"Sci. China. Life Sci."},{"key":"D5AY01285F\/cit36\/1","doi-asserted-by":"crossref","first-page":"133049","DOI":"10.1016\/j.foodchem.2022.133049","volume":"389","author":"Li","year":"2022","journal-title":"Food Chem."},{"key":"D5AY01285F\/cit37\/1","doi-asserted-by":"crossref","first-page":"16453","DOI":"10.1021\/acs.analchem.4c04710","volume":"96","author":"Wei","year":"2024","journal-title":"Anal. Chem."},{"key":"D5AY01285F\/cit38\/1","doi-asserted-by":"crossref","first-page":"3883","DOI":"10.1039\/D0CC00788A","volume":"56","author":"Yang","year":"2020","journal-title":"Chem. Commun."},{"key":"D5AY01285F\/cit39\/1","doi-asserted-by":"crossref","first-page":"1796","DOI":"10.1039\/C5CC09800A","volume":"52","author":"Liu","year":"2016","journal-title":"Chem. Commun."},{"key":"D5AY01285F\/cit40\/1","unstructured":"Protein Data Bank ,  https:\/\/www.rcsb.org\/structure\/5ID6 , accessed 10 March 2025, 2025"},{"key":"D5AY01285F\/cit41\/1","doi-asserted-by":"crossref","first-page":"4670","DOI":"10.1021\/ac980037q","volume":"70","author":"Steel","year":"1998","journal-title":"Anal. Chem."},{"key":"D5AY01285F\/cit42\/1","doi-asserted-by":"crossref","first-page":"113922","DOI":"10.1016\/j.bios.2021.113922","volume":"200","author":"Han","year":"2022","journal-title":"Biosens. Bioelectron."},{"key":"D5AY01285F\/cit43\/1","doi-asserted-by":"crossref","first-page":"162465","DOI":"10.1016\/j.cej.2025.162465","volume":"512","author":"Chen","year":"2025","journal-title":"Chem. Eng. J."},{"key":"D5AY01285F\/cit44\/1","doi-asserted-by":"crossref","first-page":"136041","DOI":"10.1016\/j.ijbiomac.2024.136041","volume":"280","author":"Li","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"key":"D5AY01285F\/cit45\/1","doi-asserted-by":"crossref","first-page":"115102","DOI":"10.1016\/j.bios.2023.115102","volume":"225","author":"Zhao","year":"2023","journal-title":"Biosens. Bioelectron."}],"container-title":["Analytical Methods"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/pubs.rsc.org\/en\/content\/articlepdf\/2025\/AY\/D5AY01285F","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T05:09:21Z","timestamp":1782882561000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.rsc.org\/ay\/article\/17\/46\/9357-9364\/880817"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025]]},"references-count":45,"journal-issue":{"issue":"46","published-print":{"date-parts":[[2025,11,27]]}},"URL":"https:\/\/doi.org\/10.1039\/d5ay01285f","relation":{},"ISSN":["1759-9660","1759-9679"],"issn-type":[{"value":"1759-9660","type":"print"},{"value":"1759-9679","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025]]}}}