{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T19:26:52Z","timestamp":1788809212085,"version":"build-2803163510"},"reference-count":46,"publisher":"Royal Society of Chemistry (RSC)","issue":"47","license":[{"start":{"date-parts":[[2026,11,24]],"date-time":"2026-11-24T00:00:00Z","timestamp":1795478400000},"content-version":"am","delay-in-days":692,"URL":"http:\/\/rsc.li\/journals-terms-of-use"}],"funder":[{"DOI":"10.13039\/100010485","name":"American Rhinologic Society","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010485","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100018421","name":"Institute for Clinical and Translational Science, University of California, Irvine","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100018421","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100016547","name":"School of Medicine, University of California, Irvine","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100016547","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["rsc.org"],"crossmark-restriction":true},"short-container-title":["Anal. Methods"],"abstract":"<jats:p>We developed a new class of E-AB biosensors capable of detecting target analytes even in the absence of complete biomarker identification and outlined essential optimization strategies to enhance signal suppression performance.<\/jats:p>","DOI":"10.1039\/d5ay01335f","type":"journal-article","created":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T14:47:26Z","timestamp":1763995646000},"page":"9649-9658","update-policy":"https:\/\/doi.org\/10.1039\/rsc_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Electrochemical aptamer-based sensor for cerebrospinal fluid detection"],"prefix":"10.1039","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-5828-7027","authenticated-orcid":false,"given":"Xinlei","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering, University of California, Irvine, CA 92697, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7366-4746","authenticated-orcid":false,"given":"Mehmet","family":"Senel","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Pharmacy, Biruni University, 34010 Istanbul, Turkiye"},{"name":"Department of Pharmaceutical Sciences, University of California, Irvine, CA 92697, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junye","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, University of California, Irvine, CA 92697, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arash","family":"Abiri","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, University of California, Irvine, CA 92697, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0632-5442","authenticated-orcid":false,"given":"Andrej","family":"Lupt\u00e1k","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, University of California, Irvine, CA 92697, USA"},{"name":"Department of Chemistry, University of California, Irvine, CA 92697, USA"},{"name":"Department of Molecular Biology and Biochemistry, University of California, Irvine, CA 92617, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank P. K.","family":"Hsu","sequence":"additional","affiliation":[{"name":"Department of Neurological Surgery, University of California, Irvine, CA 92868, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward C.","family":"Kuan","sequence":"additional","affiliation":[{"name":"Department of Neurological Surgery, University of California, Irvine, CA 92868, USA"},{"name":"Department of Otolaryngology-Head and Neck Surgery, University of California, Irvine, Orange, CA 92868, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle","family":"Khine","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, University of California, Irvine, CA 92697, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"292","published-online":{"date-parts":[[2025]]},"reference":[{"key":"D5AY01335F\/cit1\/1","doi-asserted-by":"crossref","first-page":"737217","DOI":"10.3389\/fnhum.2021.737217","volume":"15","author":"Wichmann","year":"2022","journal-title":"Front. Hum. Neurosci."},{"key":"D5AY01335F\/cit2\/1","doi-asserted-by":"crossref","first-page":"R20","DOI":"10.1152\/ajpregu.1992.262.1.R20","volume":"262","author":"Nilsson","year":"1992","journal-title":"Am. J. Physiol. Regul. Integr. Comp. Physiol."},{"key":"D5AY01335F\/cit3\/1","doi-asserted-by":"crossref","first-page":"458","DOI":"10.25259\/SNI_560_2024","volume":"15","author":"Elshanawany","year":"2024","journal-title":"Surg. Neurol. Int."},{"key":"D5AY01335F\/cit4\/1","doi-asserted-by":"crossref","first-page":"1062","DOI":"10.1007\/s00268-001-0059-7","volume":"25","author":"Friedman","year":"2001","journal-title":"World J. Surg."},{"key":"D5AY01335F\/cit5\/1","doi-asserted-by":"crossref","first-page":"598","DOI":"10.3171\/jns.1996.84.4.0598","volume":"84","author":"Schievink","year":"1996","journal-title":"J. Neurosurg."},{"key":"D5AY01335F\/cit6\/1","doi-asserted-by":"crossref","first-page":"58","DOI":"10.4103\/IJCIIS.IJCIIS_8_17","volume":"7","author":"Pillai","year":"2017","journal-title":"Int. J. Crit. Illn. Inj. Sci."},{"key":"D5AY01335F\/cit7\/1","first-page":"8797807","volume":"10","author":"Zhai","year":"2021","journal-title":"Ann. Palliat. Med."},{"key":"D5AY01335F\/cit8\/1","doi-asserted-by":"crossref","first-page":"e1272","DOI":"10.1002\/lio2.1272","volume":"9","author":"Torabi","year":"2024","journal-title":"Laryngoscope Investig. Otolaryngol."},{"key":"D5AY01335F\/cit9\/1","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1002\/lary.31845","volume":"135","author":"Eide","year":"2025","journal-title":"Laryngoscope"},{"key":"D5AY01335F\/cit10\/1","doi-asserted-by":"crossref","first-page":"168","DOI":"10.5858\/arpa.2020-0112-RA","volume":"145","author":"Park","year":"2020","journal-title":"Arch. Pathol. Lab Med."},{"key":"D5AY01335F\/cit11\/1","doi-asserted-by":"crossref","first-page":"1684","DOI":"10.1021\/acsami.1c19120","volume":"14","author":"Shao","year":"2022","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"D5AY01335F\/cit12\/1","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1177\/2472630319877377","volume":"25","author":"Bradbury","year":"2020","journal-title":"SLAS Technol."},{"key":"D5AY01335F\/cit13\/1","doi-asserted-by":"crossref","first-page":"339544","DOI":"10.1016\/j.aca.2022.339544","volume":"1196","author":"Chou","year":"2022","journal-title":"Anal. Chim. Acta"},{"key":"D5AY01335F\/cit14\/1","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1126\/science.2200121","volume":"249","author":"Tuerk","year":"1990","journal-title":"Science"},{"key":"D5AY01335F\/cit15\/1","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/j.omtn.2023.03.015","volume":"32","author":"Kichkailo","year":"2023","journal-title":"Mol. Ther.-Nucleic Acids"},{"key":"D5AY01335F\/cit16\/1","volume-title":"UC Irvine","author":"Wu","year":"2020","unstructured":"Y. X.Wu ,  UC Irvine ,  2020"},{"key":"D5AY01335F\/cit17\/1","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1039\/b921253a","volume":"135","author":"White","year":"2010","journal-title":"Analyst"},{"key":"D5AY01335F\/cit18\/1","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1146\/annurev-anchem-071015-041446","volume":"9","author":"Schoukroun-Barnes","year":"2016","journal-title":"Annu. Rev. Anal. Chem."},{"key":"D5AY01335F\/cit19\/1","first-page":"103","volume":"63","author":"Abiri","year":"2024","journal-title":"Rhin"},{"key":"D5AY01335F\/cit20\/1","volume-title":"Clinical Methods: The History, Physical, and Laboratory Examinations","author":"Busher","year":"1990","unstructured":"J. T.Busher , in  Clinical Methods: The History, Physical, and Laboratory Examinations , ed.  H. K. Walker ,  W. D. Hall  and  J. W. Hurst ,  Butterworths ,  Boston , 3rd edn,  1990"},{"key":"D5AY01335F\/cit21\/1","doi-asserted-by":"crossref","first-page":"2875","DOI":"10.1038\/nprot.2007.413","volume":"2","author":"Xiao","year":"2007","journal-title":"Nat. Protoc."},{"key":"D5AY01335F\/cit22\/1","doi-asserted-by":"crossref","first-page":"2103","DOI":"10.2147\/IJN.S189127","volume":"14","author":"Das","year":"2019","journal-title":"IJN"},{"key":"D5AY01335F\/cit23\/1","doi-asserted-by":"crossref","first-page":"1097","DOI":"10.1021\/acs.analchem.4c03423","volume":"97","author":"Liu","year":"2025","journal-title":"Anal. Chem."},{"key":"D5AY01335F\/cit24\/1","doi-asserted-by":"crossref","first-page":"10513","DOI":"10.1021\/la800801v","volume":"24","author":"White","year":"2008","journal-title":"Langmuir"},{"key":"D5AY01335F\/cit25\/1","doi-asserted-by":"crossref","first-page":"20086","DOI":"10.1021\/jacs.4c04088","volume":"146","author":"Metlay","year":"2024","journal-title":"J. Am. Chem. Soc."},{"key":"D5AY01335F\/cit26\/1","doi-asserted-by":"crossref","first-page":"1883","DOI":"10.1021\/ac7018014","volume":"80","author":"Lu","year":"2008","journal-title":"Anal. Chem."},{"key":"D5AY01335F\/cit27\/1","first-page":"534","volume":"1","author":"Schrattenecker","year":"2017","journal-title":"Proceedings"},{"key":"D5AY01335F\/cit28\/1","doi-asserted-by":"crossref","first-page":"3944","DOI":"10.1021\/ac9002358","volume":"81","author":"Bogomolova","year":"2009","journal-title":"Anal. Chem."},{"key":"D5AY01335F\/cit29\/1","doi-asserted-by":"crossref","first-page":"2221","DOI":"10.1021\/ac901794w","volume":"82","author":"Wu","year":"2010","journal-title":"Anal. Chem."},{"key":"D5AY01335F\/cit30\/1","doi-asserted-by":"crossref","first-page":"7178","DOI":"10.1021\/ac101892t","volume":"82","author":"Cai","year":"2010","journal-title":"Anal. Chem."},{"key":"D5AY01335F\/cit31\/1","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.colsurfa.2015.01.063","volume":"470","author":"Cao","year":"2015","journal-title":"Colloids Surf., A"},{"key":"D5AY01335F\/cit32\/1","doi-asserted-by":"crossref","first-page":"339742","DOI":"10.1016\/j.aca.2022.339742","volume":"1207","author":"Xie","year":"2022","journal-title":"Anal. Chim. Acta"},{"key":"D5AY01335F\/cit33\/1","doi-asserted-by":"crossref","first-page":"824","DOI":"10.1177\/0194599818789075","volume":"159","author":"Kita","year":"2018","journal-title":"Otolaryngol. Head Neck Surg."},{"key":"D5AY01335F\/cit34\/1","doi-asserted-by":"crossref","first-page":"4182","DOI":"10.7150\/thno.34411","volume":"9","author":"Oh","year":"2019","journal-title":"Theranostics"},{"key":"D5AY01335F\/cit35\/1","doi-asserted-by":"crossref","first-page":"B708","DOI":"10.1149\/2.1131908jes","volume":"166","author":"Carey","year":"2019","journal-title":"J. Electrochem. Soc."},{"key":"D5AY01335F\/cit36\/1","doi-asserted-by":"crossref","first-page":"e0206478","DOI":"10.1371\/journal.pone.0206478","volume":"13","author":"Schilde","year":"2018","journal-title":"PLoS One"},{"key":"D5AY01335F\/cit37\/1","doi-asserted-by":"crossref","first-page":"1783","DOI":"10.1111\/jog.14365","volume":"46","author":"Strawa","year":"2020","journal-title":"J. Obstet. Gynaecol. Res."},{"key":"D5AY01335F\/cit38\/1","doi-asserted-by":"crossref","first-page":"127","DOI":"10.24293\/ijcpml.v27i2.1618","volume":"27","author":"Handayani","year":"2021","journal-title":"Indones. J. Clin. Pathol. Med. Lab."},{"key":"D5AY01335F\/cit39\/1","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1053\/j.ajkd.2012.11.044","volume":"62","author":"Pavkov","year":"2013","journal-title":"Am. J. Kidney Dis."},{"key":"D5AY01335F\/cit40\/1","doi-asserted-by":"crossref","first-page":"950","DOI":"10.1039\/C8RA08642G","volume":"9","author":"Zheng","year":"2019","journal-title":"RSC Adv."},{"key":"D5AY01335F\/cit41\/1","doi-asserted-by":"crossref","first-page":"452","DOI":"10.3390\/cancers15020452","volume":"15","author":"Han\u017eek","year":"2023","journal-title":"Cancers"},{"key":"D5AY01335F\/cit42\/1","doi-asserted-by":"crossref","first-page":"109650","DOI":"10.1016\/j.microc.2023.109650","volume":"196","author":"Mohamad","year":"2024","journal-title":"Microchem. J."},{"key":"D5AY01335F\/cit43\/1","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1042\/EBC20150012","volume":"60","author":"Koczula","year":"2016","journal-title":"Essays Biochem."},{"key":"D5AY01335F\/cit44\/1","doi-asserted-by":"crossref","first-page":"6201","DOI":"10.3390\/s23136201","volume":"23","author":"Bruijns","year":"2023","journal-title":"Sensors"},{"key":"D5AY01335F\/cit45\/1","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1007\/s42242-024-00301-6","volume":"7","author":"Kim","year":"2024","journal-title":"Bio-Des. Manuf."},{"key":"D5AY01335F\/cit46\/1","doi-asserted-by":"crossref","first-page":"011601","DOI":"10.1149\/2754-2726\/ad304a","volume":"3","author":"Akhlaghi","year":"2024","journal-title":"ECS Sens. Plus"}],"container-title":["Analytical Methods"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/pubs.rsc.org\/en\/content\/articlepdf\/2025\/AY\/D5AY01335F","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T02:57:19Z","timestamp":1782874639000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.rsc.org\/ay\/article\/17\/47\/9649-9658\/880839"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025]]},"references-count":46,"journal-issue":{"issue":"47","published-print":{"date-parts":[[2025,12,4]]}},"URL":"https:\/\/doi.org\/10.1039\/d5ay01335f","relation":{},"ISSN":["1759-9660","1759-9679"],"issn-type":[{"value":"1759-9660","type":"print"},{"value":"1759-9679","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025]]}}}