{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T19:39:39Z","timestamp":1785526779184,"version":"3.56.0"},"reference-count":31,"publisher":"Royal Society of Chemistry (RSC)","issue":"48","license":[{"start":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T00:00:00Z","timestamp":1779235200000},"content-version":"am","delay-in-days":504,"URL":"http:\/\/rsc.li\/journals-terms-of-use"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22374105"],"award-info":[{"award-number":["22374105"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012542","name":"Sichuan Provincial Science and Technology Support Program","doi-asserted-by":"publisher","award":["2025NSFTD0001"],"award-info":[{"award-number":["2025NSFTD0001"]}],"id":[{"id":"10.13039\/100012542","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018545","name":"Outstanding Youth Science and Technology Talents Program of Sichuan","doi-asserted-by":"publisher","award":["903"],"award-info":[{"award-number":["903"]}],"id":[{"id":"10.13039\/501100018545","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["rsc.org"],"crossmark-restriction":true},"short-container-title":["Chem. Commun."],"abstract":"<jats:p>A CRISPR-based bioassay utilizing single-nanoparticle analysis for the sensitive detection of HIV nucleic acids, with the potential to reduce the burdensome \u2018window period\u2019 in HIV detection.<\/jats:p>","DOI":"10.1039\/d5cc01716e","type":"journal-article","created":{"date-parts":[[2025,5,20]],"date-time":"2025-05-20T05:29:36Z","timestamp":1747718976000},"page":"8759-8762","update-policy":"https:\/\/doi.org\/10.1039\/rsc_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Single nanoparticle analysis-based CRISPR\/Cas12 bioassay for amplification-free HIV detection"],"prefix":"10.1039","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-5852-493X","authenticated-orcid":false,"given":"Chengchao","family":"Zhang","sequence":"first","affiliation":[{"name":"Analytical & Testing Center, Sichuan University, Chengdu 610064, P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanlin","family":"Chen","sequence":"additional","affiliation":[{"name":"Faculty of Science, National University of Singapore, 119077, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao","family":"Chen","sequence":"additional","affiliation":[{"name":"Analytical & Testing Center, Sichuan University, Chengdu 610064, P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xu","family":"Lin","sequence":"additional","affiliation":[{"name":"Key Laboratory of Green Chemistry and Technology of Ministry of Education, College of Chemistry, Sichuan University, 29 Wangjiang Road, Chengdu 610064, P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7312-5996","authenticated-orcid":false,"given":"Zili","family":"Huang","sequence":"additional","affiliation":[{"name":"Analytical & Testing Center, Sichuan University, Chengdu 610064, P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4441-2176","authenticated-orcid":false,"given":"Lichun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Green Chemistry and Technology of Ministry of Education, College of Chemistry, Sichuan University, 29 Wangjiang Road, Chengdu 610064, P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9928-5373","authenticated-orcid":false,"given":"Rui","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Green Chemistry and Technology of Ministry of Education, College of Chemistry, Sichuan University, 29 Wangjiang Road, Chengdu 610064, P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7104-2414","authenticated-orcid":false,"given":"Yi","family":"Lv","sequence":"additional","affiliation":[{"name":"Analytical & Testing Center, Sichuan University, Chengdu 610064, P. R. China"},{"name":"Key Laboratory of Green Chemistry and Technology of Ministry of Education, College of Chemistry, Sichuan University, 29 Wangjiang Road, Chengdu 610064, P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"292","published-online":{"date-parts":[[2025]]},"reference":[{"key":"D5CC01716E\/cit1\/1","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1016\/j.bios.2017.08.042","volume":"100","author":"Diao","year":"2018","journal-title":"Biosens. Bioelectron."},{"key":"D5CC01716E\/cit2\/1","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1128\/JCM.42.1.21-29.2004","volume":"42","author":"Sickinger","year":"2004","journal-title":"J. Clin. Microbiol."},{"key":"D5CC01716E\/cit3\/1","doi-asserted-by":"crossref","first-page":"151376","DOI":"10.1016\/j.cej.2024.151376","volume":"490","author":"Cui","year":"2024","journal-title":"Chem. Eng. J."},{"key":"D5CC01716E\/cit4\/1","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1016\/S1473-3099(07)70135-4","volume":"7","author":"Hoffmann","year":"2007","journal-title":"Lancet Infect. Dis."},{"key":"D5CC01716E\/cit5\/1","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1001\/jama.2021.23487","volume":"327","author":"Tuddenham","year":"2022","journal-title":"JAMA"},{"key":"D5CC01716E\/cit6\/1","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1039\/C6LC01239F","volume":"17","author":"Mauk","year":"2017","journal-title":"Lab Chip"},{"key":"D5CC01716E\/cit7\/1","doi-asserted-by":"crossref","first-page":"112442","DOI":"10.1016\/j.bios.2020.112442","volume":"166","author":"Soares","year":"2020","journal-title":"Biosens. Bioelectron."},{"key":"D5CC01716E\/cit8\/1","year":"2024","unstructured":"WHO,  2024"},{"key":"D5CC01716E\/cit9\/1","doi-asserted-by":"crossref","first-page":"113662","DOI":"10.1016\/j.bios.2021.113662","volume":"195","author":"Liao","year":"2022","journal-title":"Biosens. Bioelectron."},{"key":"D5CC01716E\/cit10\/1","doi-asserted-by":"crossref","first-page":"13373","DOI":"10.1021\/acs.analchem.8b03052","volume":"90","author":"Zou","year":"2018","journal-title":"Anal. Chem."},{"key":"D5CC01716E\/cit11\/1","doi-asserted-by":"crossref","first-page":"117431","DOI":"10.1016\/j.trac.2023.117431","volume":"170","author":"Zhang","year":"2024","journal-title":"TrAC, Trends Anal. Chem."},{"key":"D5CC01716E\/cit12\/1","doi-asserted-by":"crossref","first-page":"38931","DOI":"10.1021\/acsami.1c09000","volume":"13","author":"Chai","year":"2021","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"D5CC01716E\/cit13\/1","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1039\/D2CS00594H","volume":"52","author":"Li","year":"2023","journal-title":"Chem. Soc. Rev."},{"key":"D5CC01716E\/cit14\/1","doi-asserted-by":"crossref","first-page":"999","DOI":"10.3390\/cells11060999","volume":"11","author":"Jamehdor","year":"2022","journal-title":"Cells"},{"key":"D5CC01716E\/cit15\/1","doi-asserted-by":"crossref","first-page":"109427","DOI":"10.1016\/j.cclet.2023.109427","volume":"35","author":"Liu","year":"2024","journal-title":"Chin. Chem. Lett."},{"key":"D5CC01716E\/cit16\/1","doi-asserted-by":"crossref","first-page":"eadd8643","DOI":"10.1126\/science.add8643","volume":"379","author":"Wang","year":"2023","journal-title":"Science"},{"key":"D5CC01716E\/cit17\/1","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1126\/science.aar6245","volume":"360","author":"Chen","year":"2018","journal-title":"Science"},{"key":"D5CC01716E\/cit18\/1","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1126\/science.aaq0179","volume":"360","author":"Gootenberg","year":"2018","journal-title":"Science"},{"key":"D5CC01716E\/cit19\/1","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1021\/acs.accounts.5b00509","volume":"49","author":"Liu","year":"2016","journal-title":"Acc. Chem. Res."},{"key":"D5CC01716E\/cit20\/1","doi-asserted-by":"crossref","first-page":"16196","DOI":"10.1021\/acs.analchem.2c03727","volume":"94","author":"Wen","year":"2022","journal-title":"Anal. Chem."},{"key":"D5CC01716E\/cit21\/1","doi-asserted-by":"crossref","first-page":"1267","DOI":"10.1016\/j.cclet.2021.07.049","volume":"33","author":"Wang","year":"2022","journal-title":"Chin. Chem. Lett."},{"key":"D5CC01716E\/cit22\/1","doi-asserted-by":"crossref","first-page":"1093","DOI":"10.1021\/acschemneuro.0c00816","volume":"12","author":"Jin","year":"2021","journal-title":"ACS Chem. Neurosci."},{"key":"D5CC01716E\/cit23\/1","doi-asserted-by":"crossref","first-page":"427","DOI":"10.46770\/AS.2023.285","volume":"44","author":"Chen","year":"2023","journal-title":"At. Spectrosc."},{"key":"D5CC01716E\/cit24\/1","doi-asserted-by":"crossref","first-page":"4952","DOI":"10.1021\/acs.analchem.0c05444","volume":"93","author":"Yin","year":"2021","journal-title":"Anal. Chem."},{"key":"D5CC01716E\/cit25\/1","doi-asserted-by":"crossref","first-page":"10665","DOI":"10.1039\/C9CC04988F","volume":"55","author":"Wang","year":"2019","journal-title":"Chem. Commun."},{"key":"D5CC01716E\/cit26\/1","doi-asserted-by":"crossref","first-page":"13075","DOI":"10.1039\/C7CC08052B","volume":"53","author":"Sun","year":"2017","journal-title":"Chem. Commun."},{"key":"D5CC01716E\/cit27\/1","doi-asserted-by":"crossref","first-page":"2330","DOI":"10.1021\/ac103265z","volume":"83","author":"Liu","year":"2011","journal-title":"Anal. Chem."},{"key":"D5CC01716E\/cit28\/1","doi-asserted-by":"crossref","first-page":"4904","DOI":"10.1021\/jacs.4c13706","volume":"147","author":"Huang","year":"2025","journal-title":"J. Am. Chem. Soc."},{"key":"D5CC01716E\/cit29\/1","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1007\/s41664-022-00232-0","volume":"6","author":"Bi","year":"2022","journal-title":"J. Anal. Test"},{"key":"D5CC01716E\/cit30\/1","doi-asserted-by":"crossref","first-page":"115229","DOI":"10.1016\/j.bios.2023.115229","volume":"229","author":"Zhan","year":"2023","journal-title":"Biosens. Bioelectron."},{"key":"D5CC01716E\/cit31\/1","doi-asserted-by":"crossref","first-page":"811","DOI":"10.1021\/acs.analchem.4c05385","volume":"97","author":"Xu","year":"2025","journal-title":"Anal. Chem."}],"container-title":["Chemical Communications"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/pubs.rsc.org\/en\/content\/articlepdf\/2025\/CC\/D5CC01716E","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T21:32:32Z","timestamp":1782941552000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.rsc.org\/cc\/article\/61\/48\/8759-8762\/882480"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025]]},"references-count":31,"journal-issue":{"issue":"48","published-print":{"date-parts":[[2025,6,10]]}},"URL":"https:\/\/doi.org\/10.1039\/d5cc01716e","relation":{},"ISSN":["1359-7345","1364-548X"],"issn-type":[{"value":"1359-7345","type":"print"},{"value":"1364-548X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025]]}}}