{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T21:08:30Z","timestamp":1791839310880,"version":"4.3.4"},"reference-count":41,"publisher":"Royal Society of Chemistry (RSC)","issue":"40","license":[{"start":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T00:00:00Z","timestamp":1758672000000},"content-version":"vor","delay-in-days":266,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100007945","name":"Steirische Wirtschaftsf\u00f6rderungsgesellschaft","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007945","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011035","name":"Standortagentur Tirol","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100011035","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004955","name":"\u00d6sterreichische Forschungsf\u00f6rderungsgesellschaft","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004955","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100018774","name":"Bundesministerium f\u00fcr Klimaschutz, Umwelt, Energie, Mobilit\u00e4t, Innovation und Technologie","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100018774","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100015053","name":"Amt der N\u00d6 Landesregierung","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100015053","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["rsc.org"],"crossmark-restriction":true},"short-container-title":["Green Chem."],"abstract":"<jats:p>\n                    We show that combining pathway and process engineering enables synthetic autotrophic\n                    <jats:italic>K. phaffii<\/jats:italic>\n                    to produce \u223c12 g L\n                    <jats:sup>\u22121<\/jats:sup>\n                    itaconic acid from CO\n                    <jats:sub>2<\/jats:sub>\n                    , highlighting the role of interplay between native and heterologous metabolic pathways and process design.\n                  <\/jats:p>","DOI":"10.1039\/d5gc03149d","type":"journal-article","created":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T11:44:52Z","timestamp":1758714292000},"page":"12715-12726","update-policy":"https:\/\/doi.org\/10.1039\/rsc_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Synthetic autotrophic yeast enables high itaconic acid production from CO\n                    <sub>2<\/sub>\n                    <i>via<\/i>\n                    integrated pathway and process design"],"prefix":"10.1039","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7157-2039","authenticated-orcid":false,"given":"\u00d6zge","family":"Ata","sequence":"first","affiliation":[{"name":"BOKU University, Vienna, Institute of Microbiology and Microbial Biotechnology, Department of Biotechnology and Food Science, 1190 Vienna, Austria"},{"name":"Austrian Centre of Industrial Biotechnology, Vienna, 1190, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9097-230X","authenticated-orcid":false,"given":"Lisa","family":"Lutz","sequence":"additional","affiliation":[{"name":"BOKU University, Vienna, Institute of Microbiology and Microbial Biotechnology, Department of Biotechnology and Food Science, 1190 Vienna, Austria"},{"name":"Austrian Centre of Industrial Biotechnology, Vienna, 1190, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0676-6002","authenticated-orcid":false,"given":"Michael","family":"Baumschabl","sequence":"additional","affiliation":[{"name":"BOKU University, Vienna, Institute of Microbiology and Microbial Biotechnology, Department of Biotechnology and Food Science, 1190 Vienna, Austria"},{"name":"Austrian Centre of Industrial Biotechnology, Vienna, 1190, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0907-4167","authenticated-orcid":false,"given":"Diethard","family":"Mattanovich","sequence":"additional","affiliation":[{"name":"BOKU University, Vienna, Institute of Microbiology and Microbial Biotechnology, Department of Biotechnology and Food Science, 1190 Vienna, Austria"},{"name":"Austrian Centre of Industrial Biotechnology, Vienna, 1190, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"292","published-online":{"date-parts":[[2025]]},"reference":[{"key":"D5GC03149D\/cit1\/1","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1016\/j.copbio.2019.10.002","volume":"62","author":"Cotton","year":"2020","journal-title":"Curr. Opin. Biotechnol"},{"key":"D5GC03149D\/cit2\/1","doi-asserted-by":"crossref","first-page":"6978","DOI":"10.1038\/s41467-023-42790-6","volume":"14","author":"Bachleitner","year":"2023","journal-title":"Nat. Commun."},{"key":"D5GC03149D\/cit3\/1","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1016\/j.synbio.2024.03.003","volume":"9","author":"Baumschabl","year":"2024","journal-title":"Synth. Syst. Biotechnol."},{"key":"D5GC03149D\/cit4\/1","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1186\/s13068-024-02549-7","volume":"17","author":"Weiler","year":"2024","journal-title":"Biotechnol. Biofuels Bioprod."},{"key":"D5GC03149D\/cit5\/1","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1186\/s12934-024-02398-x","volume":"23","author":"Jang","year":"2024","journal-title":"Microb. Cell Fact."},{"key":"D5GC03149D\/cit6\/1","doi-asserted-by":"crossref","first-page":"1879","DOI":"10.1002\/anie.201711302","volume":"57","author":"Krieg","year":"2018","journal-title":"Angew. Chem., Int. Ed."},{"key":"D5GC03149D\/cit7\/1","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1186\/s40643-023-00655-2","volume":"10","author":"Chen","year":"2023","journal-title":"Bioresour. Bioprocess."},{"key":"D5GC03149D\/cit8\/1","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1016\/j.jbiosc.2021.08.004","volume":"132","author":"Subagyo","year":"2021","journal-title":"J. Biosci. Bioeng."},{"key":"D5GC03149D\/cit9\/1","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1038\/s41587-021-01195-w","volume":"40","author":"Liew","year":"2022","journal-title":"Nat. Biotechnol."},{"key":"D5GC03149D\/cit10\/1","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/j.biortech.2015.04.043","volume":"190","author":"Phillips","year":"2015","journal-title":"Bioresour. Technol."},{"key":"D5GC03149D\/cit11\/1","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.fuel.2016.03.048","volume":"178","author":"Abubackar","year":"2016","journal-title":"Fuel"},{"key":"D5GC03149D\/cit12\/1","doi-asserted-by":"crossref","first-page":"647853","DOI":"10.3389\/fbioe.2021.647853","volume":"9","author":"Hermann","year":"2021","journal-title":"Front. Bioeng. Biotechnol."},{"key":"D5GC03149D\/cit13\/1","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1186\/1754-6834-7-99","volume":"7","author":"Angermayr","year":"2014","journal-title":"Biotechnol. Biofuels"},{"key":"D5GC03149D\/cit14\/1","doi-asserted-by":"crossref","first-page":"1447","DOI":"10.1007\/s00253-022-11770-z","volume":"106","author":"Mook","year":"2022","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"D5GC03149D\/cit15\/1","doi-asserted-by":"crossref","first-page":"139668","DOI":"10.1016\/j.cej.2022.139668","volume":"452","author":"Jin","year":"2023","journal-title":"Chem. Eng. J."},{"key":"D5GC03149D\/cit16\/1","doi-asserted-by":"crossref","first-page":"e2211827119","DOI":"10.1073\/pnas.2211827119","volume":"119","author":"Baumschabl","year":"2022","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"D5GC03149D\/cit17\/1","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1186\/s13068-021-02042-5","volume":"14","author":"Li","year":"2021","journal-title":"Biotechnol. Biofuels"},{"key":"D5GC03149D\/cit18\/1","author":"Verified Market Research","unstructured":"Verified Market Research ,  https:\/\/www.verifiedmarketresearch.com\/product\/itaconic-acid-market\/"},{"key":"D5GC03149D\/cit19\/1","doi-asserted-by":"crossref","first-page":"3574","DOI":"10.3390\/polym13203574","volume":"13","author":"Teleky","year":"2021","journal-title":"Polymers"},{"key":"D5GC03149D\/cit20\/1","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1007\/s002530100685","volume":"56","author":"Willke","year":"2001","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"D5GC03149D\/cit21\/1","doi-asserted-by":"crossref","first-page":"23","DOI":"10.3389\/fmicb.2013.00023","volume":"4","author":"Steiger","year":"2013","journal-title":"Front. Microbiol."},{"key":"D5GC03149D\/cit22\/1","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1038\/s41587-019-0363-0","volume":"38","author":"Gassler","year":"2020","journal-title":"Nat. Biotechnol."},{"key":"D5GC03149D\/cit23\/1","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/S1389-1723(02)80112-8","volume":"94","author":"Dwiart","year":"2002","journal-title":"J. Biosci. Bioeng."},{"key":"D5GC03149D\/cit24\/1","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1186\/s13068-024-02541-1","volume":"17","author":"Severinsen","year":"2024","journal-title":"Biotechnol. Biofuels Bioprod."},{"key":"D5GC03149D\/cit25\/1","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1007\/BF00398720","volume":"161","author":"Jordan","year":"1984","journal-title":"Planta"},{"key":"D5GC03149D\/cit26\/1","doi-asserted-by":"publisher","DOI":"10.1101\/2025.05.21.655372","author":"Baumschabl","year":"2025","unstructured":"M.Baumschabl ,  L.Lutz ,  M.Jecmenica ,  \u00d6.Ata  and  D.Mattanovich ,  2025 , preprint,  10.1101\/2025.05.21.655372"},{"key":"D5GC03149D\/cit27\/1","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/j.plaphy.2008.01.001","volume":"46","author":"Andersson","year":"2008","journal-title":"Plant Physiol. Biochem."},{"key":"D5GC03149D\/cit28\/1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/femsml\/uqad046","volume":"5","author":"Baumschabl","year":"2024","journal-title":"MicroLife"},{"key":"D5GC03149D\/cit29\/1","doi-asserted-by":"crossref","first-page":"1659","DOI":"10.1111\/j.1365-3040.2006.01550.x","volume":"29","author":"Yamori","year":"2006","journal-title":"Plant, Cell Environ."},{"key":"D5GC03149D\/cit30\/1","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/j.copbio.2019.09.014","volume":"62","author":"Wierckx","year":"2020","journal-title":"Curr. Opin. Biotechnol"},{"key":"D5GC03149D\/cit31\/1","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1016\/j.ymben.2021.11.007","volume":"69","author":"Gassler","year":"2022","journal-title":"Metab. Eng."},{"key":"D5GC03149D\/cit32\/1","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1186\/1475-2859-8-65","volume":"8","author":"Carnicer","year":"2009","journal-title":"Microb. Cell Fact."},{"key":"D5GC03149D\/cit33\/1","doi-asserted-by":"crossref","first-page":"4911","DOI":"10.1038\/s41467-018-07430-4","author":"Ata","year":"2018","journal-title":"Nat. Commun."},{"key":"D5GC03149D\/cit34\/1","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.procbio.2017.04.003","author":"Kim","year":"2017","journal-title":"Process Biochem."},{"key":"D5GC03149D\/cit35\/1","doi-asserted-by":"crossref","first-page":"foab009","DOI":"10.1093\/femsyr\/foab009","volume":"21","author":"Zavec","year":"2021","journal-title":"FEMS Yeast Res."},{"key":"D5GC03149D\/cit36\/1","doi-asserted-by":"crossref","first-page":"7754","DOI":"10.1038\/s41467-023-43610-7","volume":"14","author":"Mitic","year":"2023","journal-title":"Nat. Commun."},{"key":"D5GC03149D\/cit37\/1","doi-asserted-by":"crossref","first-page":"3901","DOI":"10.1007\/s00253-018-8895-7","author":"Kuenz","year":"2018","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"D5GC03149D\/cit38\/1","doi-asserted-by":"crossref","first-page":"4063","DOI":"10.1007\/s00253-017-8192-x","author":"Krull","year":"2017","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"D5GC03149D\/cit39\/1","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1186\/s13068-019-1605-6","author":"Hosseinpour Tehrani","year":"2019","journal-title":"Biotechnol. Biofuels"},{"key":"D5GC03149D\/cit40\/1","first-page":"211","volume-title":"Methods in Molecular Biology","volume":"1923","author":"Gassler","year":"2019","unstructured":"T.Gassler ,  L.Heistinger ,  D.Mattanovich ,  B.Gasser  and  R.Prielhofer , in  Methods in Molecular Biology ,  Humana Press Inc. ,  2019 , vol.  1923 , pp.  211\u2013225"},{"key":"D5GC03149D\/cit41\/1","doi-asserted-by":"crossref","first-page":"e45","DOI":"10.1093\/nar\/29.9.e45","volume":"29","author":"Pfaffl","year":"2001","journal-title":"Nucleic Acids Res."}],"container-title":["Green Chemistry"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/pubs.rsc.org\/en\/content\/articlepdf\/2025\/GC\/D5GC03149D","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T04:11:18Z","timestamp":1782879078000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.rsc.org\/gc\/article\/27\/40\/12715-12726\/894034"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025]]},"references-count":41,"journal-issue":{"issue":"40","published-print":{"date-parts":[[2025,10,13]]}},"URL":"https:\/\/doi.org\/10.1039\/d5gc03149d","relation":{"has-review":[{"id-type":"doi","id":"10.1039\/D5GC03149D\/v2\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1039\/D5GC03149D\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1039\/D5GC03149D\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1039\/D5GC03149D\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1039\/D5GC03149D\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1039\/D5GC03149D\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1039\/D5GC03149D\/v1\/decision1","asserted-by":"object"}]},"ISSN":["1463-9262","1463-9270"],"issn-type":[{"value":"1463-9262","type":"print"},{"value":"1463-9270","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025]]}}}