{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T22:09:02Z","timestamp":1787004542019,"version":"build-2736575974"},"reference-count":62,"publisher":"Royal Society of Chemistry (RSC)","issue":"23","license":[{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"vor","delay-in-days":273,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100002347","name":"Bundesministerium f\u00fcr Bildung und Forschung","doi-asserted-by":"publisher","award":["13XP5098"],"award-info":[{"award-number":["13XP5098"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001663","name":"Volkswagen Foundation","doi-asserted-by":"publisher","award":["92771"],"award-info":[{"award-number":["92771"]}],"id":[{"id":"10.13039\/501100001663","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003042","name":"Else Kr\u00f6ner-Fresenius-Stiftung","doi-asserted-by":"publisher","award":["VirChip"],"award-info":[{"award-number":["VirChip"]}],"id":[{"id":"10.13039\/501100003042","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["rsc.org"],"crossmark-restriction":true},"short-container-title":["Lab Chip"],"abstract":"<jats:p>Recent viral outbreaks have shown the need for reliable diagnostic platforms to rapidly detect various viral and bacterial pathogens at the point-of-care.<\/jats:p>","DOI":"10.1039\/d5lc00509d","type":"journal-article","created":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T11:01:48Z","timestamp":1759316508000},"page":"6324-6334","update-policy":"https:\/\/doi.org\/10.1039\/rsc_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Multiplexed detection of respiratory viral pathogens by isothermal amplification on an autonomously loaded chip at the point-of-care"],"prefix":"10.1039","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8154-0783","authenticated-orcid":false,"given":"Beatrise","family":"Berzina","sequence":"first","affiliation":[{"name":"Leibniz Institute of Polymer Research Dresden, Division Polymer Biomaterials Science, Hohe Stra\u00dfe 6, 01069 Dresden, Germany"},{"name":"TU Dresden, Center for Regenerative Therapies Dresden, Cluster of Excellence Physics of Life, and Faculty of Chemistry and Food Chemistry, Fetscherstra\u00dfe 105, 01307 Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3898-6333","authenticated-orcid":false,"given":"Krishna","family":"Gupta","sequence":"additional","affiliation":[{"name":"Leibniz Institute of Polymer Research Dresden, Division Polymer Biomaterials Science, Hohe Stra\u00dfe 6, 01069 Dresden, Germany"},{"name":"TU Dresden, Center for Regenerative Therapies Dresden, Cluster of Excellence Physics of Life, and Faculty of Chemistry and Food Chemistry, Fetscherstra\u00dfe 105, 01307 Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rayan","family":"Suliman","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology and Virology, Faculty of Medicine, Technische Universit\u00e4t Dresden, Fetscherstra\u00dfe 74, 01307 Dresden, Germany"},{"name":"Institute for Clinical Chemistry and Laboratory Medicine, University Hospital Carl Gustav Carus, Technische Universit\u00e4t Dresden, Fetscherstra\u00dfe 74, 01307 Dresden, Germany"},{"name":"Institute for Clinical Chemistry and Laboratory Medicine, St\u00e4dtisches Klinikum Dresden, Friedrichstr. 41, 01067, Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Mirtschink","sequence":"additional","affiliation":[{"name":"Institute for Clinical Chemistry and Laboratory Medicine, University Hospital Carl Gustav Carus, Technische Universit\u00e4t Dresden, Fetscherstra\u00dfe 74, 01307 Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1648-6618","authenticated-orcid":false,"given":"Alexander","family":"Dalpke","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology and Virology, Faculty of Medicine, Technische Universit\u00e4t Dresden, Fetscherstra\u00dfe 74, 01307 Dresden, Germany"},{"name":"Department of Infectious Diseases, Medical Microbiology and Hygiene, Heidelberg University, Medical Faculty Heidelberg, Im Neuenheimer Feld 324, 69120 Heidelberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0189-3448","authenticated-orcid":false,"given":"Carsten","family":"Werner","sequence":"additional","affiliation":[{"name":"Leibniz Institute of Polymer Research Dresden, Division Polymer Biomaterials Science, Hohe Stra\u00dfe 6, 01069 Dresden, Germany"},{"name":"TU Dresden, Center for Regenerative Therapies Dresden, Cluster of Excellence Physics of Life, and Faculty of Chemistry and Food Chemistry, Fetscherstra\u00dfe 105, 01307 Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2989-9879","authenticated-orcid":false,"given":"Elisha","family":"Krieg","sequence":"additional","affiliation":[{"name":"Leibniz Institute of Polymer Research Dresden, Division Polymer Biomaterials Science, Hohe Stra\u00dfe 6, 01069 Dresden, Germany"},{"name":"TU Dresden, Center for Regenerative Therapies Dresden, Cluster of Excellence Physics of Life, and Faculty of Chemistry and Food Chemistry, Fetscherstra\u00dfe 105, 01307 Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0178-1788","authenticated-orcid":false,"given":"Lars David","family":"Renner","sequence":"additional","affiliation":[{"name":"Leibniz Institute of Polymer Research Dresden, Division Polymer Biomaterials Science, Hohe Stra\u00dfe 6, 01069 Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"292","published-online":{"date-parts":[[2025]]},"reference":[{"key":"D5LC00509D\/cit1\/1","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1038\/s41586-024-08054-z","volume":"637","author":"Peacock","year":"2025","journal-title":"Nature"},{"key":"D5LC00509D\/cit2\/1","unstructured":"CDC Division of Viral Diseases. 2019-Novel Coronavirus (2019-nCoV) Real-Time RT-PCR Diagnostic Panel:Instructions for Use. CDC 2019-Novel Coronavirus (2019-nCoV) Real-Time RT-PCR Diagnostic Panel. 2023;16\u201328"},{"issue":"3","key":"D5LC00509D\/cit3\/1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.2807\/1560-7917.ES.2020.25.3.2000045","volume":"25","author":"Corman","year":"2020","journal-title":"Eurosurveillance"},{"key":"D5LC00509D\/cit4\/1","first-page":"1","volume":"2","author":"Oliveira","year":"2021","journal-title":"Front. Sens."},{"issue":"10","key":"D5LC00509D\/cit5\/1","doi-asserted-by":"crossref","first-page":"2986","DOI":"10.1038\/s41596-019-0210-2","volume":"14","author":"Kellner","year":"2019","journal-title":"Nat. Protoc."},{"issue":"546","key":"D5LC00509D\/cit6\/1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1126\/scitranslmed.abc1931","volume":"12","author":"Weissleder","year":"2020","journal-title":"Sci. Transl. Med."},{"issue":"16","key":"D5LC00509D\/cit7\/1","doi-asserted-by":"crossref","first-page":"365","DOI":"10.15585\/mmwr.mm7316a2","volume":"73","author":"Smith-Jeffcoat","year":"2024","journal-title":"MMWR Morb. Mortal. Wkly. Rep."},{"issue":"1","key":"D5LC00509D\/cit8\/1","doi-asserted-by":"crossref","first-page":"21913","DOI":"10.1038\/s41598-023-48892-x","volume":"13","author":"Katzenschlager","year":"2023","journal-title":"Sci. Rep."},{"issue":"7","key":"D5LC00509D\/cit9\/1","doi-asserted-by":"crossref","first-page":"975","DOI":"10.7326\/M23-0385","volume":"176","author":"Soni","year":"2023","journal-title":"Ann. Intern. Med."},{"issue":"44","key":"D5LC00509D\/cit10\/1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.2807\/1560-7917.ES.2021.26.44.2100441","volume":"26","author":"Scheiblauer","year":"2021","journal-title":"Eurosurveillance"},{"issue":"8","key":"D5LC00509D\/cit11\/1","doi-asserted-by":"crossref","first-page":"2201174","DOI":"10.1002\/adem.202201174","volume":"25","author":"Atceken","year":"2023","journal-title":"Adv. Eng. Mater."},{"key":"D5LC00509D\/cit12\/1","doi-asserted-by":"crossref","first-page":"116160","DOI":"10.1016\/j.trac.2020.116160","volume":"135","author":"Manmana","year":"2021","journal-title":"TrAC, Trends Anal. Chem."},{"issue":"8","key":"D5LC00509D\/cit13\/1","doi-asserted-by":"crossref","first-page":"957","DOI":"10.1038\/s41551-022-00907-0","volume":"6","author":"Zhang","year":"2022","journal-title":"Nat. Biomed. Eng."},{"issue":"4","key":"D5LC00509D\/cit14\/1","doi-asserted-by":"crossref","first-page":"e02449-16","DOI":"10.1128\/AEM.02449-16","volume":"83","author":"Renner","year":"2017","journal-title":"Appl. Environ. Microbiol."},{"issue":"1","key":"D5LC00509D\/cit15\/1","doi-asserted-by":"crossref","first-page":"1347","DOI":"10.1038\/s41598-017-00758-9","volume":"7","author":"Magro","year":"2017","journal-title":"Sci. Rep."},{"issue":"12","key":"D5LC00509D\/cit16\/1","doi-asserted-by":"crossref","first-page":"E63","DOI":"10.1093\/nar\/28.12.e63","volume":"28","author":"Notomi","year":"2000","journal-title":"Nucleic Acids Res."},{"key":"D5LC00509D\/cit17\/1","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.jviromet.2019.02.010","volume":"267","author":"Takayama","year":"2019","journal-title":"J. Virol. Methods"},{"issue":"7","key":"D5LC00509D\/cit18\/1","doi-asserted-by":"crossref","first-page":"e204","DOI":"10.1371\/journal.pbio.0040204","volume":"4","author":"Piepenburg","year":"2006","journal-title":"PLoS Biol."},{"issue":"4","key":"D5LC00509D\/cit19\/1","doi-asserted-by":"crossref","first-page":"602","DOI":"10.1002\/jmv.20498","volume":"77","author":"Keightley","year":"2005","journal-title":"J. Med. Virol."},{"key":"D5LC00509D\/cit20\/1","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1038\/350091a0","volume":"350","author":"Compton","year":"1991","journal-title":"Nature"},{"issue":"4","key":"D5LC00509D\/cit21\/1","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1007\/s13206-022-00080-1","volume":"16","author":"Lee","year":"2022","journal-title":"BioChip J."},{"issue":"10","key":"D5LC00509D\/cit22\/1","doi-asserted-by":"crossref","first-page":"4641","DOI":"10.1073\/pnas.92.10.4641","volume":"92","author":"Fire","year":"1995","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"D5LC00509D\/cit23\/1","doi-asserted-by":"crossref","first-page":"113207","DOI":"10.1016\/j.bios.2021.113207","volume":"183","author":"Azhar","year":"2021","journal-title":"Biosens. Bioelectron."},{"issue":"1","key":"D5LC00509D\/cit24\/1","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1186\/s12967-021-02741-5","volume":"19","author":"Sun","year":"2021","journal-title":"J. Transl. Med."},{"issue":"1","key":"D5LC00509D\/cit25\/1","doi-asserted-by":"crossref","first-page":"ysy018","DOI":"10.1093\/synbio\/ysy018","volume":"3","author":"Ma","year":"2018","journal-title":"Synth. Biol."},{"issue":"4","key":"D5LC00509D\/cit26\/1","doi-asserted-by":"crossref","first-page":"925","DOI":"10.1016\/j.cell.2014.10.002","volume":"159","author":"Green","year":"2014","journal-title":"Cell"},{"issue":"12","key":"D5LC00509D\/cit27\/1","doi-asserted-by":"crossref","first-page":"1451","DOI":"10.1038\/s41587-020-0571-7","volume":"38","author":"Jung","year":"2020","journal-title":"Nat. Biotechnol."},{"issue":"13","key":"D5LC00509D\/cit28\/1","doi-asserted-by":"crossref","first-page":"5820","DOI":"10.1021\/jacs.1c11706","volume":"144","author":"Patino Diaz","year":"2022","journal-title":"J. Am. Chem. Soc."},{"issue":"17","key":"D5LC00509D\/cit29\/1","doi-asserted-by":"crossref","first-page":"e80","DOI":"10.1093\/nar\/gkae680","volume":"52","author":"Gupta","year":"2024","journal-title":"Nucleic Acids Res."},{"issue":"3","key":"D5LC00509D\/cit30\/1","doi-asserted-by":"crossref","first-page":"730","DOI":"10.1021\/acssensors.1c02079","volume":"7","author":"Dong","year":"2022","journal-title":"ACS Sens."},{"key":"D5LC00509D\/cit31\/1","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.eclinm.2019.02.008","volume":"8","author":"Dou","year":"2019","journal-title":"EClinicalMedicine"},{"issue":"1","key":"D5LC00509D\/cit32\/1","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1128\/JCM.43.1.427-430.2005","volume":"43","author":"Poon","year":"2005","journal-title":"J. Clin. Microbiol."},{"issue":"9","key":"D5LC00509D\/cit33\/1","doi-asserted-by":"crossref","first-page":"1634","DOI":"10.1039\/D0LC01156H","volume":"21","author":"Song","year":"2021","journal-title":"Lab\nChip"},{"issue":"8","key":"D5LC00509D\/cit34\/1","doi-asserted-by":"crossref","first-page":"2826","DOI":"10.3390\/ijms21082826","volume":"21","author":"Lu","year":"2020","journal-title":"Int. J. Mol. Sci."},{"issue":"37","key":"D5LC00509D\/cit35\/1","doi-asserted-by":"crossref","first-page":"22727","DOI":"10.1073\/pnas.2014739117","volume":"117","author":"Ganguli","year":"2020","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"7","key":"D5LC00509D\/cit36\/1","doi-asserted-by":"crossref","first-page":"1297","DOI":"10.1039\/D2LC00021K","volume":"22","author":"Lim","year":"2022","journal-title":"Lab Chip"},{"issue":"15","key":"D5LC00509D\/cit37\/1","doi-asserted-by":"crossref","first-page":"2932","DOI":"10.1039\/D1LC00266J","volume":"21","author":"Soares","year":"2021","journal-title":"Lab Chip"},{"issue":"2","key":"D5LC00509D\/cit38\/1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/jcm.01785-21","volume":"60","author":"Savela","year":"2022","journal-title":"J. Clin. Microbiol."},{"key":"D5LC00509D\/cit39\/1","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1016\/j.aca.2020.09.013","volume":"1135","author":"Morbioli","year":"2020","journal-title":"Anal. Chim. Acta"},{"issue":"1","key":"D5LC00509D\/cit40\/1","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1146\/annurev.matsci.28.1.153","volume":"28","author":"Xia","year":"1998","journal-title":"Annu. Rev. Mater. Sci."},{"issue":"6","key":"D5LC00509D\/cit41\/1","doi-asserted-by":"crossref","first-page":"1052","DOI":"10.1039\/C2LC20887C","volume":"12","author":"Cira","year":"2012","journal-title":"Lab Chip"},{"issue":"3","key":"D5LC00509D\/cit42\/1","doi-asserted-by":"crossref","first-page":"178","DOI":"10.2144\/btn-2020-0078","volume":"69","author":"Zhang","year":"2020","journal-title":"BioTechniques"},{"issue":"16","key":"D5LC00509D\/cit43\/1","doi-asserted-by":"crossref","first-page":"4685","DOI":"10.1007\/s00216-022-04090-8","volume":"414","author":"Jang","year":"2022","journal-title":"Anal. Bioanal. Chem."},{"key":"D5LC00509D\/cit44\/1","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/nmeth.2019","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nat. Methods"},{"issue":"3","key":"D5LC00509D\/cit45\/1","first-page":"147","volume":"21","author":"Puhach","year":"2023","journal-title":"Nat. Rev. Microbiol."},{"issue":"37","key":"D5LC00509D\/cit46\/1","doi-asserted-by":"crossref","first-page":"22727","DOI":"10.1073\/pnas.2014739117","volume":"117","author":"Ganguli","year":"2020","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"4","key":"D5LC00509D\/cit47\/1","doi-asserted-by":"crossref","first-page":"950","DOI":"10.1111\/1751-7915.13586","volume":"13","author":"Huang","year":"2020","journal-title":"Microb. Biotechnol."},{"key":"D5LC00509D\/cit48\/1","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.jviromet.2017.02.013","volume":"244","author":"Carter","year":"2017","journal-title":"J. Virol. Methods"},{"issue":"3","key":"D5LC00509D\/cit49\/1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.pntd.0003578","volume":"9","author":"Hayashida","year":"2015","journal-title":"PLoS Neglected Trop. Dis."},{"issue":"21","key":"D5LC00509D\/cit50\/1","doi-asserted-by":"crossref","first-page":"6875","DOI":"10.1039\/D0AN01098G","volume":"145","author":"Kumar","year":"2020","journal-title":"Analyst"},{"key":"D5LC00509D\/cit51\/1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fmicb.2019.00001","volume":"10","author":"Quyen","year":"2019","journal-title":"Front. Microbiol."},{"key":"D5LC00509D\/cit52\/1","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/j.mimet.2015.11.004","volume":"119","author":"Seyrig","year":"2015","journal-title":"J. Microbiol. Methods"},{"issue":"39","key":"D5LC00509D\/cit53\/1","doi-asserted-by":"crossref","first-page":"24450","DOI":"10.1073\/pnas.2011221117","volume":"117","author":"Rabe","year":"2020","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"1","key":"D5LC00509D\/cit54\/1","doi-asserted-by":"crossref","first-page":"5921","DOI":"10.1038\/s41467-020-19097-x","volume":"11","author":"Arizti-Sanz","year":"2020","journal-title":"Nat. Commun."},{"issue":"2","key":"D5LC00509D\/cit55\/1","first-page":"1","volume":"11","author":"Cui","year":"2016","journal-title":"PLoS One"},{"issue":"1","key":"D5LC00509D\/cit56\/1","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1016\/j.jviromet.2011.11.012","volume":"179","author":"Do","year":"2012","journal-title":"J. Virol. Methods"},{"issue":"1","key":"D5LC00509D\/cit57\/1","doi-asserted-by":"crossref","first-page":"S45","DOI":"10.1093\/infdis\/jiac163","volume":"226","author":"McGinley","year":"2022","journal-title":"J. Infect. Dis."},{"issue":"7","key":"D5LC00509D\/cit58\/1","doi-asserted-by":"crossref","first-page":"996","DOI":"10.1093\/infdis\/jir494","volume":"204","author":"El Saleeby","year":"2011","journal-title":"J. Infect. Dis."},{"issue":"4","key":"D5LC00509D\/cit59\/1","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1007\/s10544-023-00676-w","volume":"25","author":"Mytzka","year":"2023","journal-title":"Biomed. Microdevices"},{"issue":"1","key":"D5LC00509D\/cit60\/1","doi-asserted-by":"crossref","first-page":"478","DOI":"10.1016\/j.snb.2010.07.021","volume":"150","author":"Sasaki","year":"2010","journal-title":"Sens. Actuators, B"},{"issue":"21","key":"D5LC00509D\/cit61\/1","doi-asserted-by":"crossref","first-page":"11219","DOI":"10.1021\/acs.analchem.7b02478","volume":"89","author":"Chen","year":"2017","journal-title":"Anal. Chem."},{"issue":"17","key":"D5LC00509D\/cit62\/1","doi-asserted-by":"crossref","first-page":"3838","DOI":"10.1039\/D2AN00438K","volume":"147","author":"Jankelow","year":"2022","journal-title":"Analyst"}],"container-title":["Lab on a Chip"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/pubs.rsc.org\/en\/content\/articlepdf\/2025\/LC\/D5LC00509D","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T21:26:07Z","timestamp":1782941167000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.rsc.org\/lc\/article\/25\/23\/6324-6334\/895358"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025]]},"references-count":62,"journal-issue":{"issue":"23","published-print":{"date-parts":[[2025,11,18]]}},"URL":"https:\/\/doi.org\/10.1039\/d5lc00509d","relation":{"has-review":[{"id-type":"doi","id":"10.1039\/D5LC00509D\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1039\/D5LC00509D\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1039\/D5LC00509D\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1039\/D5LC00509D\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1039\/D5LC00509D\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1039\/D5LC00509D\/v1\/decision1","asserted-by":"object"}]},"ISSN":["1473-0197","1473-0189"],"issn-type":[{"value":"1473-0197","type":"print"},{"value":"1473-0189","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025]]}}}