{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T19:26:56Z","timestamp":1788809216799,"version":"build-2803163510"},"reference-count":42,"publisher":"Royal Society of Chemistry (RSC)","issue":"3","license":[{"start":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T00:00:00Z","timestamp":1763424000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100009994","name":"Excellent Youth Foundation of Jiangsu Scientific Committee","doi-asserted-by":"publisher","award":["BK20220060"],"award-info":[{"award-number":["BK20220060"]}],"id":[{"id":"10.13039\/501100009994","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009994","name":"Excellent Youth Foundation of Jiangsu Scientific Committee","doi-asserted-by":"publisher","award":["JSSCTD202348"],"award-info":[{"award-number":["JSSCTD202348"]}],"id":[{"id":"10.13039\/501100009994","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32171452"],"award-info":[{"award-number":["32171452"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82373456"],"award-info":[{"award-number":["82373456"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002949","name":"Government of Jiangsu Province","doi-asserted-by":"publisher","award":["SJCX24_2025"],"award-info":[{"award-number":["SJCX24_2025"]}],"id":[{"id":"10.13039\/501100002949","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005054","name":"Nantong University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2024YFA1613800"],"award-info":[{"award-number":["2024YFA1613800"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009156","name":"Nantong Municipal Science and Technology Bureau","doi-asserted-by":"publisher","award":["MS2024023"],"award-info":[{"award-number":["MS2024023"]}],"id":[{"id":"10.13039\/501100009156","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["rsc.org"],"crossmark-restriction":true},"short-container-title":["Chem. Sci."],"abstract":"<jats:p>This study developed an ultrasensitive ratiometric fluorescent sensor based on the integration of Cas13a and FLAPs, enabling amplification-free detection of HBV and HCV.<\/jats:p>","DOI":"10.1039\/d5sc05526a","type":"journal-article","created":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T00:40:52Z","timestamp":1763426452000},"page":"1656-1665","update-policy":"https:\/\/doi.org\/10.1039\/rsc_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Enhanced detection of HBV and HCV using Cas13a-FLAP and FGoAI platforms"],"prefix":"10.1039","volume":"17","author":[{"given":"Xijuan","family":"Gu","sequence":"first","affiliation":[{"name":"Jiangsu Key Laboratory of Advanced Medical Analysis and Public Health, School of Public Health, Nantong University, Nantong, Jiangsu 226019, P. R. China"},{"name":"Xinglin College, Nantong University, Qidong, Jiangsu 226236, P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianyi","family":"Wang","sequence":"additional","affiliation":[{"name":"Affiliated hospital of Nantong University, Nantong University, Nantong, Jiangsu 226019, P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingwei","family":"Wu","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Advanced Medical Analysis and Public Health, School of Public Health, Nantong University, Nantong, Jiangsu 226019, P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiayun","family":"Guan","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Advanced Medical Analysis and Public Health, School of Public Health, Nantong University, Nantong, Jiangsu 226019, P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoxia","family":"Kang","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Advanced Medical Analysis and Public Health, School of Public Health, Nantong University, Nantong, Jiangsu 226019, P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjun","family":"Ming","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Advanced Medical Analysis and Public Health, School of Public Health, Nantong University, Nantong, Jiangsu 226019, P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yidan","family":"Zhu","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Advanced Medical Analysis and Public Health, School of Public Health, Nantong University, Nantong, Jiangsu 226019, P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-1884-489X","authenticated-orcid":false,"given":"Qian","family":"Xu","sequence":"additional","affiliation":[{"name":"School of Artificial Intelligence and Computer Science, Nantong University, Nantong, Jiangsu 226019, P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2819-8592","authenticated-orcid":false,"given":"Yuling","family":"Qin","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Advanced Medical Analysis and Public Health, School of Public Health, Nantong University, Nantong, Jiangsu 226019, P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4118-7970","authenticated-orcid":false,"given":"Li","family":"Wu","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Advanced Medical Analysis and Public Health, School of Public Health, Nantong University, Nantong, Jiangsu 226019, P. R. China"},{"name":"Affiliated hospital of Nantong University, Nantong University, Nantong, Jiangsu 226019, P. R. China"},{"name":"School of Life Sciences, Nantong University, Nantong, Jiangsu 226019, P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"292","published-online":{"date-parts":[[2026]]},"reference":[{"key":"D5SC05526A\/cit1\/1","first-page":"100792","volume":"36","author":"Thomadakis","year":"2024","journal-title":"Lancet Reg. Health-Eu."},{"key":"D5SC05526A\/cit2\/1","first-page":"332","volume":"8","author":"Cui","year":"2023","journal-title":"Lancet Gastroenterol."},{"key":"D5SC05526A\/cit3\/1","doi-asserted-by":"crossref","first-page":"2041","DOI":"10.1056\/NEJMra1810477","volume":"380","author":"Thomas","year":"2019","journal-title":"N. Engl. J. Med."},{"key":"D5SC05526A\/cit4\/1","doi-asserted-by":"crossref","first-page":"1517","DOI":"10.3390\/jpm12091517","volume":"12","author":"An","year":"2022","journal-title":"J. Pers. Med."},{"key":"D5SC05526A\/cit5\/1","doi-asserted-by":"crossref","first-page":"e2219170","DOI":"10.1001\/jamanetworkopen.2022.19170","volume":"5","author":"Ly","year":"2022","journal-title":"JAMA Netw. Open"},{"key":"D5SC05526A\/cit6\/1","doi-asserted-by":"crossref","first-page":"e2142282","DOI":"10.1001\/jamanetworkopen.2021.42282","volume":"5","author":"Migdal","year":"2022","journal-title":"JAMA Netw. Open"},{"key":"D5SC05526A\/cit7\/1","doi-asserted-by":"crossref","first-page":"909","DOI":"10.1186\/s12879-024-09786-z","volume":"24","author":"Ye","year":"2024","journal-title":"BMC Infect. Dis."},{"key":"D5SC05526A\/cit8\/1","doi-asserted-by":"crossref","first-page":"1015","DOI":"10.1111\/liv.15548","volume":"43","author":"Soriano","year":"2023","journal-title":"Liver Int."},{"key":"D5SC05526A\/cit9\/1","doi-asserted-by":"crossref","first-page":"113155","DOI":"10.1016\/j.bios.2021.113155","volume":"181","author":"Ga\u0148ov\u00e1","year":"2021","journal-title":"Biosens. Bioelectron."},{"key":"D5SC05526A\/cit10\/1","doi-asserted-by":"crossref","first-page":"10304","DOI":"10.1021\/acs.analchem.2c02470","volume":"94","author":"Dong","year":"2022","journal-title":"Anal. Chem."},{"key":"D5SC05526A\/cit11\/1","doi-asserted-by":"crossref","first-page":"7899","DOI":"10.1021\/acsnano.1c02981","volume":"15","author":"Valera","year":"2021","journal-title":"ACS Nano"},{"key":"D5SC05526A\/cit12\/1","doi-asserted-by":"crossref","first-page":"7797","DOI":"10.1021\/acs.analchem.0c05170","volume":"93","author":"Ganguli","year":"2021","journal-title":"Anal. Chem."},{"key":"D5SC05526A\/cit13\/1","doi-asserted-by":"crossref","first-page":"e2307959","DOI":"10.1002\/smll.202307959","volume":"20","author":"Lim","year":"2024","journal-title":"Small"},{"key":"D5SC05526A\/cit14\/1","doi-asserted-by":"crossref","first-page":"116400","DOI":"10.1016\/j.bios.2024.116400","volume":"259","author":"Zeng","year":"2024","journal-title":"Biosens. Bioelectron."},{"key":"D5SC05526A\/cit15\/1","doi-asserted-by":"crossref","first-page":"1818","DOI":"10.1038\/s41467-024-46001-8","volume":"15","author":"Deng","year":"2024","journal-title":"Nat. Commun."},{"key":"D5SC05526A\/cit16\/1","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1126\/science.aam9321","volume":"356","author":"Gootenberg","year":"2017","journal-title":"Science"},{"key":"D5SC05526A\/cit17\/1","doi-asserted-by":"crossref","first-page":"116449","DOI":"10.1016\/j.bios.2024.116449","volume":"261","author":"Gu","year":"2024","journal-title":"Biosens. Bioelectron."},{"key":"D5SC05526A\/cit18\/1","doi-asserted-by":"crossref","first-page":"33505","DOI":"10.1021\/acsnano.4c10823","volume":"18","author":"Zhao","year":"2024","journal-title":"ACS Nano"},{"key":"D5SC05526A\/cit19\/1","doi-asserted-by":"crossref","first-page":"870","DOI":"10.1038\/s41587-020-0513-4","volume":"38","author":"Broughton","year":"2020","journal-title":"Nat. Biotechnol."},{"key":"D5SC05526A\/cit20\/1","doi-asserted-by":"crossref","first-page":"1166","DOI":"10.1038\/s41467-025-56516-3","volume":"16","author":"Cheng","year":"2025","journal-title":"Nat. Commun."},{"key":"D5SC05526A\/cit21\/1","doi-asserted-by":"crossref","first-page":"885","DOI":"10.1038\/s41589-023-01534-9","volume":"20","author":"Tong","year":"2024","journal-title":"Nat. Chem. Biol."},{"key":"D5SC05526A\/cit22\/1","first-page":"115929","volume":"247","author":"Hui","year":"2023","journal-title":"Biosens. Bioelectron."},{"key":"D5SC05526A\/cit23\/1","doi-asserted-by":"crossref","first-page":"116268","DOI":"10.1016\/j.bios.2024.116268","volume":"257","author":"Wang","year":"2024","journal-title":"Biosens. Bioelectron."},{"key":"D5SC05526A\/cit24\/1","doi-asserted-by":"crossref","first-page":"10927","DOI":"10.1021\/acs.analchem.4c00962","volume":"96","author":"Cao","year":"2024","journal-title":"Anal. Chem."},{"key":"D5SC05526A\/cit25\/1","doi-asserted-by":"crossref","first-page":"8819834","DOI":"10.1155\/2024\/8819834","volume":"2024","author":"Shahni","year":"2024","journal-title":"J. Nucleic Acids"},{"key":"D5SC05526A\/cit26\/1","doi-asserted-by":"crossref","first-page":"4842","DOI":"10.3390\/ijms22094842","volume":"22","author":"Ding","year":"2021","journal-title":"Int. J. Mol. Sci."},{"key":"D5SC05526A\/cit27\/1","doi-asserted-by":"crossref","first-page":"1524","DOI":"10.3390\/diagnostics12071524","volume":"12","author":"Kham-Kjing","year":"2022","journal-title":"Diagnostics"},{"key":"D5SC05526A\/cit28\/1","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1007\/s12072-022-10311-0","volume":"16","author":"Zhang","year":"2022","journal-title":"Hepatol. Int."},{"key":"D5SC05526A\/cit29\/1","doi-asserted-by":"crossref","first-page":"e202203826","DOI":"10.1002\/anie.202203826","volume":"61","author":"Li","year":"2022","journal-title":"Angew. Chem. Int. Edit"},{"key":"D5SC05526A\/cit30\/1","doi-asserted-by":"crossref","first-page":"2046","DOI":"10.1039\/D3CC05300H","volume":"60","author":"Feng","year":"2024","journal-title":"Chem. Commun."},{"key":"D5SC05526A\/cit31\/1","doi-asserted-by":"crossref","first-page":"1287","DOI":"10.1038\/s41587-019-0249-1","volume":"37","author":"Chen","year":"2019","journal-title":"Nat. Biotechnol."},{"key":"D5SC05526A\/cit32\/1","doi-asserted-by":"crossref","first-page":"eabc7802","DOI":"10.1126\/sciadv.abc7802","volume":"7","author":"Shi","year":"2021","journal-title":"Sci. Adv."},{"key":"D5SC05526A\/cit33\/1","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1007\/s00604-025-07284-w","volume":"192","author":"Lai","year":"2025","journal-title":"Microchim. Acta"},{"key":"D5SC05526A\/cit34\/1","doi-asserted-by":"crossref","first-page":"116628","DOI":"10.1016\/j.bios.2024.116628","volume":"264","author":"Kang","year":"2024","journal-title":"Biosens. Bioelectron."},{"key":"D5SC05526A\/cit35\/1","doi-asserted-by":"crossref","first-page":"18096","DOI":"10.1021\/acs.analchem.4c03974","volume":"96","author":"Li","year":"2024","journal-title":"Anal. Chem."},{"key":"D5SC05526A\/cit36\/1","doi-asserted-by":"crossref","first-page":"18411","DOI":"10.1039\/D4SC05226A","volume":"15","author":"Gu","year":"2024","journal-title":"Chem. Sci."},{"key":"D5SC05526A\/cit37\/1","doi-asserted-by":"crossref","first-page":"5597","DOI":"10.1038\/s41467-025-60917-9","volume":"16","author":"Lin","year":"2025","journal-title":"Nat. Commun."},{"key":"D5SC05526A\/cit38\/1","doi-asserted-by":"crossref","first-page":"1168","DOI":"10.1038\/s41551-020-00617-5","volume":"4","author":"Woo","year":"2020","journal-title":"Nat. Biomed. Eng."},{"key":"D5SC05526A\/cit39\/1","doi-asserted-by":"crossref","first-page":"114071","DOI":"10.1016\/j.bios.2022.114071","volume":"204","author":"Park","year":"2022","journal-title":"Biosens. Bioelectron."},{"key":"D5SC05526A\/cit40\/1","first-page":"976","volume":"95","author":"Endoh","year":"2023","journal-title":"Anal. Chem."},{"key":"D5SC05526A\/cit41\/1","doi-asserted-by":"crossref","first-page":"e67","DOI":"10.1093\/nar\/gkae551","volume":"52","author":"Zheng","year":"2024","journal-title":"Nucleic Acids Res."},{"key":"D5SC05526A\/cit42\/1","doi-asserted-by":"crossref","first-page":"4729","DOI":"10.1039\/D2LC00857B","volume":"22","author":"Qin","year":"2022","journal-title":"Lab Chip"}],"container-title":["Chemical Science"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/pubs.rsc.org\/en\/content\/articlepdf\/2026\/SC\/D5SC05526A","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T17:24:31Z","timestamp":1782926671000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.rsc.org\/sc\/article\/17\/3\/1656-1665\/911009"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"references-count":42,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,1,21]]}},"URL":"https:\/\/doi.org\/10.1039\/d5sc05526a","relation":{},"ISSN":["2041-6520","2041-6539"],"issn-type":[{"value":"2041-6520","type":"print"},{"value":"2041-6539","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026]]}}}