{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T19:36:35Z","timestamp":1782934595544,"version":"3.54.5"},"reference-count":23,"publisher":"Royal Society of Chemistry (RSC)","issue":"20","license":[{"start":{"date-parts":[[2027,5,6]],"date-time":"2027-05-06T00:00:00Z","timestamp":1809561600000},"content-version":"am","delay-in-days":490,"URL":"http:\/\/rsc.li\/journals-terms-of-use"}],"content-domain":{"domain":["rsc.org"],"crossmark-restriction":true},"short-container-title":["Anal. Methods"],"abstract":"<jats:p>MicroRNAs (miRNAs) have emerged as promising biomarkers for early diagnosis and management of degenerative disc disease (DDD); however, their low abundance and high sequence homology pose significant challenges for clinical detection.<\/jats:p>","DOI":"10.1039\/d6ay00578k","type":"journal-article","created":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T15:45:24Z","timestamp":1778082324000},"page":"4154-4160","update-policy":"https:\/\/doi.org\/10.1039\/rsc_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Simple yet sensitive MicroRNA detection using allosteric probe-initiated triple amplification and Cas13a\/crRNA-based amplification reaction"],"prefix":"10.1039","volume":"18","author":[{"given":"He","family":"Chen","sequence":"first","affiliation":[{"name":"Wenzhou TCM Hospital of Zhejiang Chinese Medical University, Orthopedics and Traumatology, Wenzhou City, Zhejiang Province, 325000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-6928-1830","authenticated-orcid":false,"given":"Chaonan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Wenzhou TCM Hospital of Zhejiang Chinese Medical University, Orthopedics and Traumatology, Wenzhou City, Zhejiang Province, 325000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"292","published-online":{"date-parts":[[2026]]},"reference":[{"issue":"11","key":"D6AY00578K\/cit1\/1","doi-asserted-by":"crossref","first-page":"1810","DOI":"10.3390\/jpm12111810","volume":"12","author":"Scarcia","year":"2022","journal-title":"J. Pers. Med."},{"issue":"10","key":"D6AY00578K\/cit2\/1","doi-asserted-by":"crossref","first-page":"1213","DOI":"10.1007\/s00256-012-1446-x","volume":"41","author":"Charran","year":"2012","journal-title":"Skelet. Radiol."},{"issue":"6","key":"D6AY00578K\/cit3\/1","doi-asserted-by":"crossref","first-page":"421","DOI":"10.5455\/medarh.2019.73.421-424","volume":"73","author":"Kos","year":"2019","journal-title":"Med. Arch."},{"issue":"9","key":"D6AY00578K\/cit4\/1","doi-asserted-by":"crossref","first-page":"E499","DOI":"10.1097\/BRS.0000000000003322","volume":"45","author":"Divi","year":"2020","journal-title":"Spine"},{"issue":"1","key":"D6AY00578K\/cit5\/1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/bib\/bbs075","volume":"15","author":"Liu","year":"2014","journal-title":"Briefings Bioinf."},{"issue":"11","key":"D6AY00578K\/cit6\/1","doi-asserted-by":"crossref","first-page":"853","DOI":"10.1016\/j.tips.2019.09.007","volume":"40","author":"Lu","year":"2019","journal-title":"Trends Pharmacol. Sci."},{"key":"D6AY00578K\/cit7\/1","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1111\/j.1749-6632.2010.05866.x","volume":"1215","author":"Mukhopadhyay","year":"2011","journal-title":"Ann. N. Y. Acad. Sci."},{"issue":"4","key":"D6AY00578K\/cit8\/1","doi-asserted-by":"crossref","first-page":"1117","DOI":"10.1007\/s00216-008-2570-2","volume":"394","author":"Li","year":"2009","journal-title":"Anal. Bioanal. Chem."},{"issue":"8","key":"D6AY00578K\/cit9\/1","doi-asserted-by":"crossref","first-page":"2226","DOI":"10.1039\/C4OB02104E","volume":"13","author":"Tian","year":"2015","journal-title":"Org. Biomol. Chem."},{"issue":"2","key":"D6AY00578K\/cit10\/1","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1038\/nprot.2007.528","volume":"3","author":"V\u00e1rallyay","year":"2008","journal-title":"Nat. Protoc."},{"issue":"32","key":"D6AY00578K\/cit11\/1","doi-asserted-by":"crossref","first-page":"5498","DOI":"10.1002\/anie.201001375","volume":"49","author":"Jia","year":"2010","journal-title":"Angew Chem. Int. Ed. Engl."},{"key":"D6AY00578K\/cit12\/1","first-page":"7107","author":"Juan","year":"2018","journal-title":"Anal. Chem."},{"issue":"15","key":"D6AY00578K\/cit13\/1","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1093\/nar\/gkx588","volume":"45","author":"Peter","year":"2017","journal-title":"Nucleic Acids Res."},{"issue":"Pt 2","key":"D6AY00578K\/cit14\/1","doi-asserted-by":"crossref","first-page":"125130","DOI":"10.1016\/j.talanta.2023.125130","volume":"266","author":"Long","year":"2024","journal-title":"Talanta"},{"key":"D6AY00578K\/cit15\/1","doi-asserted-by":"crossref","first-page":"116695","DOI":"10.1016\/j.bios.2024.116695","volume":"266","author":"Huang","year":"2024","journal-title":"Biosens. Bioelectron."},{"issue":"10","key":"D6AY00578K\/cit16\/1","doi-asserted-by":"crossref","first-page":"3324","DOI":"10.1039\/c3cs60439j","volume":"43","author":"Ali","year":"2014","journal-title":"Chem. Soc. Rev."},{"issue":"34","key":"D6AY00578K\/cit17\/1","doi-asserted-by":"crossref","first-page":"6330","DOI":"10.1002\/anie.200705982","volume":"47","author":"Zhao","year":"2008","journal-title":"Angew Chem. Int. Ed. Engl."},{"issue":"1","key":"D6AY00578K\/cit18\/1","doi-asserted-by":"crossref","first-page":"5012","DOI":"10.1038\/s41467-018-07324-5","volume":"9","author":"Zhou","year":"2018","journal-title":"Nat. Commun."},{"issue":"2020","key":"D6AY00578K\/cit19\/1","doi-asserted-by":"crossref","first-page":"105239","DOI":"10.1016\/j.microc.2020.105239","volume":"158","author":"Zhang","year":"2020","journal-title":"Microchem. J."},{"issue":"16","key":"D6AY00578K\/cit20\/1","doi-asserted-by":"crossref","first-page":"e202203412","DOI":"10.1002\/chem.202203412","volume":"29","author":"Zhang","year":"2023","journal-title":"Chemistry"},{"key":"D6AY00578K\/cit21\/1","doi-asserted-by":"crossref","first-page":"124582","DOI":"10.1016\/j.talanta.2023.124582","volume":"260","author":"Zhao","year":"2023","journal-title":"Talanta"},{"issue":"7","key":"D6AY00578K\/cit22\/1","doi-asserted-by":"crossref","first-page":"1655","DOI":"10.3390\/cells10071655","volume":"10","author":"Granados-Riveron","year":"2021","journal-title":"Cells"},{"key":"D6AY00578K\/cit23\/1","doi-asserted-by":"crossref","first-page":"1060947","DOI":"10.3389\/fmicb.2022.1060947","volume":"13","author":"Zhao","year":"2022","journal-title":"Front. Microbiol."}],"container-title":["Analytical Methods"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/pubs.rsc.org\/en\/content\/articlepdf\/2026\/AY\/D6AY00578K","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T17:47:43Z","timestamp":1782928063000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.rsc.org\/ay\/article\/18\/20\/4154-4160\/1225190"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"references-count":23,"journal-issue":{"issue":"20","published-print":{"date-parts":[[2026,5,28]]}},"URL":"https:\/\/doi.org\/10.1039\/d6ay00578k","relation":{},"ISSN":["1759-9660","1759-9679"],"issn-type":[{"value":"1759-9660","type":"print"},{"value":"1759-9679","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026]]}}}